An early effect of the S component of staphylococcal leukocidin on methylation of phospholipid in various leukocytes.

Abstract:

:On incubation of rabbit polymorphonuclear leukocytes with the S component of staphylococcal leukocidin at 37 degrees C, the 3H-labeled methyl group of S-adenosyl[methyl-3H]methionine was rapidly incorporated into phospholipid. Subsequently, the methylated phosphatidylcholine was degraded by activated phospholipase A2. Complete blockage of the methylation of phospholipid by a mixture of erythro-9-[2-hydroxy-3-nonyl]adenine, adenosine, and L-homocysteine thiolactone markedly inhibited the activation of phospholipase A2 by the S component. It also inhibited the binding of 125I-labeled F component to the cells, but not that of the labeled S component. These results suggest that methylation of phospholipid in the cell membranes by the S component results in activation of phospholipase A2, which induces the binding of the F component to the cells.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Noda M,Hirayama T,Matsuda F,Kato I

doi

10.1128/IAI.50.1.142-145.1985

subject

Has Abstract

pub_date

1985-10-01 00:00:00

pages

142-5

issue

1

eissn

0019-9567

issn

1098-5522

journal_volume

50

pub_type

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