Recognition of the galactose- or N-acetylgalactosamine-binding lectin of Entamoeba histolytica by human immune sera.

Abstract:

:Cure of amebic liver abscess is associated with resistance to recurrent invasive amebiasis and the development of a humoral and cell-mediated immune response. We determined whether human immune sera contain blocking antibody for the 170-kilodalton (kDa) galactose or N-acetylgalactosamine (Gal/GalNAc)-binding lectin of Entamoeba histolytica. By Western blot (immunoblot) of whole amebae subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all eight immune sera studied here prominently recognized a 170-kDa amebic protein. Western blot of the purified Gal/GalNAc lectin with pooled human immune sera (PHIS) confirmed that the 170-kDa band was the adherence lectin. Immunoprecipitation of [35S]methionine-metabolically-labeled amebae with the antilectin monoclonal antibody H8-5 and with PHIS demonstrated that the 170-kDa lectin was the major antigen recognized by PHIS. The in vitro adherence of E. histolytica trophozoites to CHO cells at 4 degrees C was inhibited by prior exposure of amebae to greater than or equal to 1.0% PHIS. The humoral response to the Gal/GalNAc-binding lectin of the parasite may contribute to the development of protective immunity against invasive amebiasis.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Petri WA Jr,Joyce MP,Broman J,Smith RD,Murphy CF,Ravdin JI

doi

10.1128/IAI.55.10.2327-2331.1987

subject

Has Abstract

pub_date

1987-10-01 00:00:00

pages

2327-31

issue

10

eissn

0019-9567

issn

1098-5522

journal_volume

55

pub_type

杂志文章
  • Murine neonates infected with Yersinia enterocolitica develop rapid and robust proinflammatory responses in intestinal lymphoid tissues.

    abstract::Neonatal animals are generally very susceptible to infection with bacterial pathogens. However, we recently reported that neonatal mice are highly resistant to orogastric infection with Yersinia enterocolitica. Here, we show that proinflammatory responses greatly exceeding those in adults arise very rapidly in the mes...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01489-13

    authors: Siefker DT,Echeverry A,Brambilla R,Fukata M,Schesser K,Adkins B

    更新日期:2014-02-01 00:00:00

  • Cell-associated levan of Actinomyces viscosus.

    abstract::Actinomyces viscosus ATCC 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. Levan was not detectable on the surface of glucose-grown A. viscosus, but after a brief incubation of these cells with 5% sucrose, they were en...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.19.2.711-719.1978

    authors: Warner TN,Miller CH

    更新日期:1978-02-01 00:00:00

  • Interstrain conservation of babesial RAP-1 surface-exposed B-cell epitopes despite rap-1 genomic polymorphism.

    abstract::Members of the babesial rhoptry-associated protein 1 (RAP-1) family express surface-exposed B-cell epitopes and are candidate antigens for vaccine development. The relationship between rap-1 genomic polymorphism and surface-exposed B-cell epitope expression was analyzed by comparison of biological clones of Mexico str...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.8.3576-3579.1994

    authors: Suarez CE,McElwain TF,Echaide I,Torioni de Echaide S,Palmer GH

    更新日期:1994-08-01 00:00:00

  • Identification and characterization of a novel genomic island integrated at selC in locus of enterocyte effacement-negative, Shiga toxin-producing Escherichia coli.

    abstract::The selC tRNA gene is a common site for the insertion of pathogenicity islands in a variety of bacterial enteric pathogens. We demonstrate here that Escherichia coli that produces Shiga toxin 2d and does not harbor the locus of enterocyte effacement (LEE) contains, instead, a novel genomic island. In one representativ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.11.6863-6873.2001

    authors: Schmidt H,Zhang WL,Hemmrich U,Jelacic S,Brunder W,Tarr PI,Dobrindt U,Hacker J,Karch H

    更新日期:2001-11-01 00:00:00

  • The flagella of an atypical enteropathogenic Escherichia coli strain are required for efficient interaction with and stimulation of interleukin-8 production by enterocytes in vitro.

    abstract::The ability of some typical enteropathogenic Escherichia coli (EPEC) strains to adhere to, invade, and increase interleukin-8 (IL-8) production in intestinal epithelial cells in vitro has been demonstrated. However, few studies regarding these aspects have been performed with atypical EPEC (aEPEC) strains, which are e...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00177-09

    authors: Sampaio SC,Gomes TA,Pichon C,du Merle L,Guadagnini S,Abe CM,Sampaio JL,Le Bouguénec C

    更新日期:2009-10-01 00:00:00

  • Molecular characterization of the Escherichia coli asymptomatic bacteriuria strain 83972: the taming of a pathogen.

    abstract::Escherichia coli 83972 is a clinical asymptomatia bacteriuric isolate that is able to colonize the human urinary bladder without inducing an immune response. Here we demonstrate that one of the mechanisms by which this strain has become attenuated is through the mutation of its genes encoding type 1 and P fimbriae. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.74.1.781-785.2006

    authors: Klemm P,Roos V,Ulett GC,Svanborg C,Schembri MA

    更新日期:2006-01-01 00:00:00

  • PD-1 deficiency enhances humoral immunity of malaria infection treatment vaccine.

    abstract::Malaria infection treatment vaccine (ITV) is a promising strategy to induce homologous and heterologous protective immunity against the blood stage of the parasite. However, the underlying mechanism of protection remains largely unknown. Here, we found that a malaria-specific antibody (Ab) could mediate the protective...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02621-14

    authors: Liu T,Lu X,Zhao C,Fu X,Zhao T,Xu W

    更新日期:2015-05-01 00:00:00

  • A second merozoite surface protein (MSP-4) of Plasmodium falciparum that contains an epidermal growth factor-like domain.

    abstract::Merozoite surface proteins of Plasmodium falciparum play a critical role in the invasion of human erythrocytes by the malaria parasite. Here we describe the identification of a novel protein with a molecular mass of 40 kDa that is found on the merozoite surface of P. falciparum. We call this protein merozoite surface ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.11.4460-4467.1997

    authors: Marshall VM,Silva A,Foley M,Cranmer S,Wang L,McColl DJ,Kemp DJ,Coppel RL

    更新日期:1997-11-01 00:00:00

  • In vitro lymphocyte responses of coccidioidin skin test-positive and -negative persons to coccidioidin, spherulin, and a coccidioides cell wall antigen.

    abstract::The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived e...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.15.3.751-755.1977

    authors: Cox RA,Brummer E,Lecara G

    更新日期:1977-03-01 00:00:00

  • Cell-mediated immunity to Herpes simplex in humans: lymphocyte cytotoxicity measured by 51-Cr release from infected cells.

    abstract::We assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. Paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. This was demons...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.2.355-359.1975

    authors: Russell AS,Percy JS,Kovithavongs T

    更新日期:1975-02-01 00:00:00

  • Concomitant loss of conformation and superantigenic activity of staphylococcal enterotoxin B deletion mutant proteins.

    abstract::The T-cell-stimulating activity of staphylococcal enterotoxin B (SEB) is an important factor in the pathogenesis of certain staphylococcal diseases. To investigate the immunologically active domains of the SEB molecule, we have produced truncated fragments of recombinant SEB by C-terminal and N-terminal deletions. The...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.6.2445-2452.1993

    authors: Metzroth B,Marx T,Linnig M,Fleischer B

    更新日期:1993-06-01 00:00:00

  • Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Characterization of the Experimental System.

    abstract::The macrophage migration inhibition test was applied to the study of delayed hypersensitivity in mice vaccinated intravenously with oil-treated cell walls of Mycobacterium bovis BCG. Migration inhibition of peritoneal exudate cells from sensitized mice was demonstrated directly upon incubation of the cells with purifi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.1.6.587-594.1970

    authors: Yamamoto K,Anacker RL

    更新日期:1970-06-01 00:00:00

  • Lymphokine-induced mycobacteriostatic activity in mouse pleural macrophages.

    abstract::We have developed an in vitro assay to study the intramacrophage replication of Mycobacterium tuberculosis or Mycobacterium bovis BCG. In this assay, extracellular mycobacterial replication was avoided by using a small infecting inoculum and a new method for efficiently removing nonphagocytocytized bacilli. The abilit...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.2.786-793.1982

    authors: Zlotnik A,Crowle AJ

    更新日期:1982-08-01 00:00:00

  • Studies of the rickettsial plaque assay technique.

    abstract::A plaque assay system for pathogenic rickettsiae, which utilizes primary chick embryo tissue cultures, is described. It proved to be a highly reproducible measure of infectiousness for Rickettsia rickettsi and R. typhi, which were employed in most studies; as well as for R. canada, R. prowazeki, R. sibirica, R. akari,...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.5.715-722.1972

    authors: Wike DA,Tallent G,Peacock MG,Ormsbee RA

    更新日期:1972-05-01 00:00:00

  • Ehrlichia chaffeensis and Anaplasma phagocytophilum lack genes for lipid A biosynthesis and incorporate cholesterol for their survival.

    abstract::Ehrlichia chaffeensis and Anaplasma phagocytophilum are agents of human monocytic and granulocytic ehrlichioses, respectively. They are extremely sensitive to mechanical stress and are pleomorphic gram-negative bacteria. Membrane incorporation of cholesterol from the eukaryotic host is known to be essential for other ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.9.5324-5331.2003

    authors: Lin M,Rikihisa Y

    更新日期:2003-09-01 00:00:00

  • Characterization of a linear epitope on Chlamydia trachomatis serovar L2 DnaK-like protein.

    abstract::A cytoplasmic 75-kDa immunogen from Chlamydia trachomatis serovar L2 has previously been characterized as being similar to the Escherichia coli heat shock protein DnaK. We have localized a linear epitope for one monoclonal antibody specific for C. trachomatis DnaK. By use of a recombinant DNA technique, the epitope wa...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.5.2051-2057.1994

    authors: Birkelund S,Larsen B,Holm A,Lundemose AG,Christiansen G

    更新日期:1994-05-01 00:00:00

  • Apple flavonoid phloretin inhibits Escherichia coli O157:H7 biofilm formation and ameliorates colon inflammation in rats.

    abstract::Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents, while commensal biofilms often fortify the host's immune system. Hence, controlling biofilm formation of both pathogenic bacteria and commensal bacteria is important in bacterium-related diseases. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.05580-11

    authors: Lee JH,Regmi SC,Kim JA,Cho MH,Yun H,Lee CS,Lee J

    更新日期:2011-12-01 00:00:00

  • Roles of OspA, OspB, and flagellin in protective immunity to Lyme borreliosis in laboratory mice.

    abstract::Vaccination with recombinant outer surface protein A (OspA) has been shown to protect mice from infection with Borrelia burgdorferi, the Lyme disease agent. To determine whether antibodies to B. burgdorferi proteins other than OspA are involved in protective immunity, antibodies to OspA were removed from protective an...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.2.657-661.1992

    authors: Fikrig E,Barthold SW,Marcantonio N,Deponte K,Kantor FS,Flavell RA

    更新日期:1992-02-01 00:00:00

  • Infection-immunity in tularemia: specificity of cellular immunity.

    abstract::The relationship between hypersensitivity and cellular resistance to infection with facultative intracellular parasites was studied in mice by using infection-immunity in tularemia as a model system. Delayed hypersensitivity to antigenic fractions of Francisella tularensis was first detected 6 to 7 days after immuniza...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.3.311-318.1972

    authors: Claflin JL,Larson CL

    更新日期:1972-03-01 00:00:00

  • Assay, characterization, and localization of an enterotoxin produced by Salmonella.

    abstract::An enterotoxic factor isolated from cultures of Salmonella yielded reproducible results in the suckling mouse model in contrast to other animal models. The enterotoxin appears to possess properties similar to both the heat-stable and heat-labile enterotoxins of Escherichia coli. Preliminary results indicate that the t...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.1.14-22.1975

    authors: Koupal LR,Deibel RH

    更新日期:1975-01-01 00:00:00

  • Temperature-sensitive mutant of coxsackievirus B3 establishes resistance in neonatal mice that protects them during adolescence against coxsackievirus B3-induced myocarditis.

    abstract::Inoculation of neonatal CD-1 mice by multiple routes with an amyocarditic temperature-sensitive (ts) mutant (ts 1) derived from a myocarditic parent variant of coxsackievirus B3 (CVB3(m)) resulted in approximately half of the neonates surviving to adolescence. Challenge of the ts 1 survivors with CVB3(m) did not induc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.39.2.851-864.1983

    authors: Gauntt CJ,Paque RE,Trousdale MD,Gudvangen RJ,Barr DT,Lipotich GJ,Nealon TJ,Duffey PS

    更新日期:1983-02-01 00:00:00

  • Neisseria lactamica and Neisseria meningitidis share lipooligosaccharide epitopes but lack common capsular and class 1, 2, and 3 protein epitopes.

    abstract::Neisseria lactamica, a common human pharyngeal commensal, contributes to acquired immunity to Neisseria meningitidis. To define the surface antigens shared between these two species, we used monoclonal antibodies (MAbs) to study 35 N. lactamica strains isolated in various parts of the world for cross-reactivity with m...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.2.602-608.1989

    authors: Kim JJ,Mandrell RE,Griffiss JM

    更新日期:1989-02-01 00:00:00

  • Effects of local immunization with glucosyltransferase on colonization of hamsters by Streptococcus mutans.

    abstract::Experiments were performed to study the effect of antibody to Streptococcus mutans glucosyltransferase (GTF) on the implantation of these organisms in hamsters. Salivary (immunoglobulin A) and serum (immunoglobulin G) antibodies to GTF and GTF-inhibiting activity were elicited by injection of GTF in Freund complete ad...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.2.656-661.1982

    authors: Smith DJ,Taubman MA,Ebersole JL

    更新日期:1982-08-01 00:00:00

  • Inbred strains derived from feral mice reveal new pathogenic mechanisms of experimental leishmaniasis due to Leishmania major.

    abstract::Two inbred mouse strains, derived from feral founders, are susceptible to experimental leishmaniasis due to Leishmania major and support a disease of a severity intermediate between those observed in strains C57BL/6 and BALB/c. Mice of the MAI strain develop a severe, nonhealing, but nonfatal disease with no resistanc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.8.4603-4611.2004

    authors: Babay BE,Louzir H,Kebaïer C,Boubaker S,Dellagi K,Cazenave PA

    更新日期:2004-08-01 00:00:00

  • Identification and functional characterization of chicken toll-like receptor 5 reveals a fundamental role in the biology of infection with Salmonella enterica serovar typhimurium.

    abstract::Toll-like receptors (TLRs) are a major component of the pattern recognition receptor repertoire that detect invading microorganisms and direct the vertebrate immune system to eliminate infection. In chickens, the differential biology of Salmonella serovars (systemic versus gut-restricted localization) correlates with ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.4.2344-2350.2005

    authors: Iqbal M,Philbin VJ,Withanage GS,Wigley P,Beal RK,Goodchild MJ,Barrow P,McConnell I,Maskell DJ,Young J,Bumstead N,Boyd Y,Smith AL

    更新日期:2005-04-01 00:00:00

  • Estimation of growth parameters for some oral bacteria grown in continuous culture under glucose-limiting conditions.

    abstract::The coexistence of bacteria in natural environments can often be explained in terms of competition for a growth-limiting substrate(s), and the outcome of such competition depends upon relevant growth parameters such as substrate affinity and yield. Dental plaque bacteria are frequently carbon and energy limited. Growt...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.52.3.897-901.1986

    authors: Rogers AH,de Jong MH,Zilm PS,van der Hoeven JS

    更新日期:1986-06-01 00:00:00

  • Characterization of porcine intestinal receptors for the K88ac fimbrial adhesin of Escherichia coli as mucin-type sialoglycoproteins.

    abstract::We have previously identified two K88ac adhesion receptors (210 and 240 kDa) which are present in membrane preparations from adhesive but not nonadhesive porcine intestinal brush border cells; these adhesin receptors are postulated to be important determinants of the susceptibility of pigs to K88ac+ enterotoxigenic Es...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.12.5404-5410.1994

    authors: Erickson AK,Baker DR,Bosworth BT,Casey TA,Benfield DA,Francis DH

    更新日期:1994-12-01 00:00:00

  • Adhesion-dependent activation of mucosal interleukin-6 production.

    abstract::Mucosal exposure to Escherichia coli elicits an inflammatory response in the urinary tract. Interleukin-6 (IL-6) is secreted into the urine, and polymorphonuclear leukocytes (PMNLs) are recruited to the site of infection. This study analyzed the ability of mucosally administered bacterial components to activate IL-6 a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.12.4357-4362.1991

    authors: Linder H,Engberg I,Hoschützky H,Mattsby-Baltzer I,Svanborg C

    更新日期:1991-12-01 00:00:00

  • Analysis of cells of the mononuclear phagocyte series in experimental mycobacterial granulomas by monoclonal antibodies.

    abstract::Two distinct types of granulomas were produced in the draining lymph nodes by immunizing guinea pigs with Mycobacterium bovis BCG or Mycobacterium leprae, as reported earlier (Narayanan et al., J. Pathol. 134:253-265, 1981). In the BCG-induced granuloma there is successful containment, killing, and degradation of the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.39.1.344-352.1983

    authors: Mathew RC,Katayama I,Gupta SK,Curtis J,Turk JL

    更新日期:1983-01-01 00:00:00

  • Alveolar bone loss in rats after immunization with Actinomyces viscosus.

    abstract::We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We mea...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.31.3.971-977.1981

    authors: Burckhardt JJ,Gaegauf-Zollinger R,Schmid R,Guggenheim B

    更新日期:1981-03-01 00:00:00