Abstract:
:Actinomyces viscosus ATCC 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. Levan was not detectable on the surface of glucose-grown A. viscosus, but after a brief incubation of these cells with 5% sucrose, they were encapsulated with tenaciously adhering levan. The levan layer constituted between 0.02 and 0.03% of the cell dry weight. In contrast, sucrose-grown A. viscosus cells possessed a low level of cell-associated levan, which was only moderately increased by incubation in sucrose and which partially existed as a loose slime rather than a tenacious capsule.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Warner TN,Miller CHdoi
10.1128/IAI.19.2.711-719.1978subject
Has Abstractpub_date
1978-02-01 00:00:00pages
711-9issue
2eissn
0019-9567issn
1098-5522journal_volume
19pub_type
杂志文章abstract::Escherichia coli heat-stable enterotoxin (ST) binds to receptors on rat intestinal cells and brush border membranes (BBM). We devised experiments to examine the reversibility of ST binding. We found that both 125I-labeled ST and native ST were spontaneously dissociable from the BBM receptor. Radiolabeled ST bound to B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.329-334.1987
更新日期:1987-02-01 00:00:00
abstract::Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chroma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.982-986.1976
更新日期:1976-03-01 00:00:00
abstract::Pasteurella (Mannheimia) haemolytica leukotoxin (Lkt) causes cell type- and species-specific effects in ruminant leukocytes. Recent studies indicate that P. haemolytica Lkt binds to bovine CD18, the common subunit of all beta2 integrins. We designed experiments with the following objectives: to identify which member o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.72-79.2000
更新日期:2000-01-01 00:00:00
abstract::Burkholderia cepacia is an opportunistic pathogen that causes severe systemic infections in patients with chronic granulomatous disease (CGD) or with cystic fibrosis (CF), but its mechanisms of virulence are poorly understood. We developed a murine model of systemic infection in wild-type (WT) and gamma interferon kno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4027-4032.1999
更新日期:1999-08-01 00:00:00
abstract::A recombinant 23-kDa protein (rBPI23) derived from human bactericidal/permeability-increasing protein (BPI) possesses potent endotoxin-neutralizing abilities in vitro and in vivo. Binding of rBPI23 to those endotoxins (lipopolysaccharides [LPSs]) encountered clinically would be a prerequisite for efficacy in decreasin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3564-3567.1994
更新日期:1994-08-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). A murine UTI model has revealed an infection cascade whereby UPEC undergoes cycles of invasion of the bladder epithelium, intracellular proliferation in polysaccharide-containing biofilm-like masses called intracellular bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00925-09
更新日期:2010-03-01 00:00:00
abstract::Arthritis is a frequent and major complication of infection with Borrelia burgdorferi sensu stricto. The antigens responsible for the induction of arthritis are unknown. Here we provide direct evidence that a major surface protein, outer surface protein A (OspA), can induce arthritis. Hamsters were vaccinated with 30,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.658-663.2000
更新日期:2000-02-01 00:00:00
abstract::The adenylate cyclase-hemolysin of Bordetella pertussis consists of a cell-invasive N-terminal adenylate cyclase domain linked to a C-terminal RTX hemolysin containing extensive glycine-rich repeats. The toxin is an essential virulence factor required in the initial stages of infection. Adenylate cyclase-hemolysin was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3309-3315.1995
更新日期:1995-09-01 00:00:00
abstract::Histoplasma capsulatum induces a cell-mediated immune response in the lungs and lymphoid organs of mammals. In this study, we analyzed the progression of the cytokine and inflammatory reactions in the lungs of mice infected intranasally with H. capsulatum. We measured cytokine mRNA levels and determined the inflammato...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1473-1481.1998
更新日期:1998-04-01 00:00:00
abstract::Pertussis toxin is secreted from Bordetella pertussis with the assistance of the Ptl transport system, a member of the type IV family of macromolecular transporters. The S1 subunit and the B oligomer combine to form the holotoxin prior to export from the bacterial cell, although the site of assembly is not known. To b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1193-1201.2002
更新日期:2002-03-01 00:00:00
abstract::The two published sequences of the pertussis toxin operon differ in 3 bp in the S1 subunit gene. In this report, we provide evidence that Bordetella pertussis strains are able to produce active pertussis toxin only when they contain one of the two possible nucleotide sequences. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.1177-1179.1991
更新日期:1991-03-01 00:00:00
abstract::Synthetic peptides and murine monoclonal antibodies were used to map cross-reactive chlamydial epitopes. A species-specific epitope in the central region of variable sequence region 4 abuts the amino-terminal end of a B-serogroup-specific or F/G-serogroup-specific epitope, which in turn abuts known serovar-specific ep...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2839-2841.1996
更新日期:1996-07-01 00:00:00
abstract::We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We mea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.971-977.1981
更新日期:1981-03-01 00:00:00
abstract::The antigenic relationship of glucosyltransferases (GTF) produced by different serotypes of Streptococcus mutans was studied by using a functional inhibition assay. Rat, rabbit, or hamster immune fluids, directed to cell-associated or supernatant-derived GTF, were tested against ammonium sulfate-precipitated culture s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.91-103.1977
更新日期:1977-01-01 00:00:00
abstract::Coagulation and inflammation are interconnected, suggesting that coagulation plays a key role in the inflammatory response to pathogens. A phenome-wide association study (PheWAS) was used to identify clinical phenotypes of patients with a polymorphism in coagulation factor X. Patients with this single nucleotide polym...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00031-19
更新日期:2019-04-23 00:00:00
abstract::Phospholipase A2 and lysophospholipase activities were detected in the culture supernatant fluids of a virulent strain of Vibrio vulnificus. The phospholipase A2 was inactivated by heating at 56 degrees C for 30 min, had an apparent molecular weight of greater than or equal to 80,000 (estimated by gel filtration with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.458-463.1984
更新日期:1984-08-01 00:00:00
abstract::Mice given Mycobacterium tuberculosis bacilli via the respiratory route succumbed much sooner to infection than mice given 1,000 times more bacilli via the intravenous route. Vaccination provided increased protection to an M. tuberculosis challenge infection; however, mice infected via the respiratory route remained m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::The protozoan parasite Giardia duodenalis (Giardia lamblia) is one of the most commonly found intestinal pathogens in mammals, including humans. In the current study, a Giardia muris-mouse model was used to analyze cytokine transcription patterns and histological changes in intestinal tissue at different time points d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01536-14
更新日期:2014-08-01 00:00:00
abstract::We report the cloning and expression of Ac-GST-1, a novel glutathione S-transferase from the adult hookworm Ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. The predicted Ac-GST-1 open reading frame contains 207 amino acids (mass, 24 kDa) and exhibited up to 65% amino acid ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6903-6911.2005
更新日期:2005-10-01 00:00:00
abstract::Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as solid-phase ant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.215-220.1994
更新日期:1994-01-01 00:00:00
abstract::Recent studies revealed that acetylation is a widely used protein modification in prokaryotic organisms. The major protein acetylation acetyltransferase YfiQ and the sirtuin-like deacetylase CobB have been found to be involved in basic physiological processes, such as primary metabolism, chemotaxis, and stress respons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-18
更新日期:2018-05-22 00:00:00
abstract::Lipopolysaccharide (LPS) has recently been shown to facilitate macrophage foam cell formation and has been suggested to be a proatherogenic factor. The mechanism of LPS induced cholesterol accumulation, however, is unclear. In this report, using the macrophage-like RAW 264.7 cell line, we provide experimental evidence...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2995-3003.2002
更新日期:2002-06-01 00:00:00
abstract::Recent studies have shown that probiotics are beneficial in T-cell-mediated inflammatory diseases. The molecular mechanism by which probiotics work remains elusive, but accumulating evidence indicates that probiotics can modulate immune cell responses. Since T cells express receptors for bacterial products or componen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01172-06
更新日期:2007-04-01 00:00:00
abstract::Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.511-517.1984
更新日期:1984-08-01 00:00:00
abstract::We used inhibitors of receptor-mediated endocytosis to study the mechanisms of infectivity, especially the uptake mechanism, of Chlamydia trachomatis for cultured cells. The effect of methylamine and monodansylcadaverine on the different stages of the chlamydial growth cycle in McCoy cells was examined. There was a do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.534-541.1983
更新日期:1983-05-01 00:00:00
abstract::Monoclonal antibodies to the capsular polysaccharide of Cryptococcus neoformans produce distinct capsular reactions and have biological activities that are determined by serotype specificity. In the present study, polyclonal rabbit anticapsular antibodies were cross-absorbed to produce serotype specificities similar t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.1189-1191.2001
更新日期:2001-02-01 00:00:00
abstract::Intra-abdominal polymicrobial infections cause significant morbidity and mortality. An experimental mouse model of Candida albicans-Staphylococcus aureus intra-abdominal infection (IAI) results in 100% mortality by 48 to 72 h postinoculation, while monomicrobial infections are avirulent. Mortality is associated with r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-15
更新日期:2015-10-19 00:00:00
abstract::A receptor moiety and blood group substance recognized by the O75X adhesin was studied. Well-defined erythrocytes representing different blood group systems and bacterial derivatives carrying plasmid pBJN406 encoding the adhesin were used in a direct hemagglutination assay. We showed that Dr blood group antigen, a com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1057-1060.1988
更新日期:1988-05-01 00:00:00
abstract::Ribosomal subunits from Mycobacterium smegmatis were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. These s(20.w) values for the subunits are 48.7S and 28.1S. The molecular weight of the 49S subunit is about 1.65 x 10(6), and that of the 28S subunit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.489-496.1974
更新日期:1974-03-01 00:00:00
abstract::Broth culture supernatants from Tox+ Helicobacter pylori strains induce vacuolation of HeLa cells in vitro and contain VacA in concentrations that are higher than those found in supernatants from Tox- H. pylori strains. To investigate the basis for this phenomenon, we analyzed the transcription of the vacuolating cyto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3088-3094.1998
更新日期:1998-07-01 00:00:00