Abstract:
:Nonspecific cross-reacting antigen (NCA) is a well-characterized membrane glycoprotein on granulocytes, macrophages, and lung epithelium. Structural studies at the protein and genomic levels have revealed that NCA is a member of the immunoglobulin supergene family, and hybridization studies showed that the transcript level of NCA is induced by treatment with gamma interferon. These studies, as well as the expression of NCA on granulocytes, suggest a role for NCA in immune response. For a first step in studying this possible role, we have examined the binding of two glycoforms of NCA designated NCA-50 (Mr, 50,000) and TEX-75 (Mr, 75,000). Here we report the results from binding assays which demonstrate carbohydrate-mediated binding of Escherichia coli expressing type 1 fimbriae and of isolated type 1 fimbriae to NCA-50. TEX-75 did not bind to the purified fimbriae but bound slightly to the bacterial strain. Inhibition studies showed that the binding to NCA-50 involved interaction of mannose moieties on NCA-50 and lectins on the fimbriae. The binding of NCA-50 to bacterial fimbriae was confirmed by electron microscopy studies, using immunolabeling techniques. In addition, we show that the surface expression of NCA-50 (and presumably of other NCA species) on isolated polymorphonuclear leukocytes is increased following activation with the bacterial peptide formylmethionyl-leucyl-phenylalanine, consistent with a role for NCA in immune response.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Sauter SL,Rutherfurd SM,Wagener C,Shively JE,Hefta SAdoi
10.1128/IAI.59.7.2485-2493.1991subject
Has Abstractpub_date
1991-07-01 00:00:00pages
2485-93issue
7eissn
0019-9567issn
1098-5522journal_volume
59pub_type
杂志文章abstract::T-cell-mediated immunity is an important determinant in protection against primary infection with Coccidioides immitis, a dimorphic fungal pathogen that causes the disease coccidioidomycosis. To determine if interleukin-12 (IL-12) gene therapy could potentiate host response against C. immitis, we constructed a single-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2996-3001.1999
更新日期:1999-06-01 00:00:00
abstract::The regulation of innate immune responses to pathogens occurs through the interaction of Toll-like receptors (TLRs) with pathogen-associated molecular patterns and the activation of several signaling pathways whose contribution to the overall innate immune response to pathogens is poorly understood. We demonstrate a m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00492-07
更新日期:2007-10-01 00:00:00
abstract::The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (deterg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1825-1828.1988
更新日期:1988-07-01 00:00:00
abstract::Analysis of the partition in a two-polymer phase system of Salmonella typhimurium R mutants with different susceptibility to phagocytosis distinguished between the mutants. Close to 80% of the R mutants least resistant to phagocytosis accumulated in the dextran-rich phase, whereas more phagocytosis-resistant mutants s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.1.36-41.1973
更新日期:1973-07-01 00:00:00
abstract::Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5384-5387.1993
更新日期:1993-12-01 00:00:00
abstract::In order to construct an effector strain for the replacement therapy of dental caries, we wished to combine the properties of low-level acid production and high-level colonization potential in a strain of Streptococcus mutans. To this end, we made a deletion in the lactate dehydrogenase (LDH) gene cloned from the bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.60-64.1994
更新日期:1994-01-01 00:00:00
abstract::The importance of bacterial phospholipases during serum-mediated killing of Escherichia coli was examined by using wild-type DR+ DS+ and an isogenic phospholipase-deficient mutant DR- DS-. No difference in serum sensitivity was observed when the parental DR+ DS+ and mutant DR- DS- strains were exposed to various conce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.183-188.1977
更新日期:1977-10-01 00:00:00
abstract::The role of receptor binding in the toxicity, immunogenicity, and adjuvanticity of the heat-labile enterotoxin of Escherichia coli (LT) was examined by comparing native LT and LT(G33D), a B-subunit receptor binding mutant, with respect to the ability to bind to galactose and to GM1, toxicity on mouse Y-1 adrenal tumor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.4943-4950.1997
更新日期:1997-12-01 00:00:00
abstract::A ruthenium red-staining capsule was observed on two pathogenic strains, but not on one nonpathogenic strain of Mycoplasma gallisepticum. The capsule appeared to mediate cytadsorption of mycoplasmas to the chicken tracheal epithelium without evidence of membrane fusion. No relationship was seen between the presence of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.2.830-833.1982
更新日期:1982-05-01 00:00:00
abstract::We have performed microarray hybridization studies on 40 clinical isolates from 12 common serovars within Salmonella enterica subspecies I to identify the conserved chromosomal gene pool. We were able to separate the core invariant portion of the genome by a novel mathematical approach using a decision tree based on g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.7894-7905.2005
更新日期:2005-12-01 00:00:00
abstract::CD8+ T cells are essential for protection against mycobacteria, as is clearly demonstrated by the fatal outcome of experimental infection of beta-2 microglobulin knockout mice. However, the mechanisms and antigens (Ags) leading to CD8+ T-cell activation and regulation have been poorly characterized. Here we show that,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.676-684.1997
更新日期:1997-02-01 00:00:00
abstract::To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of ML...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3724-3729.1993
更新日期:1993-09-01 00:00:00
abstract::We compared a cryptococcal culture filtrate antigen referred to as CneF with chemically defined cryptococcal antigen fractions isolated by Cherniak and co-workers by using double immunodiffusion gels, polyacrylamide gel electrophoresis, immunoblots, and footpad reactivity of immunized mice. The three previously descri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.2.424-431.1988
更新日期:1988-02-01 00:00:00
abstract::We measured the microbicidal peptides MCP-1 and MCP-2 in rabbit alveolar macrophages (AM), comparing rabbits pretreated with complete Freund adjuvant with untreated control animals. Levels of MCP-1 increased from 4.7 +/- 0.6 microgram/10(7) resident AM to 13.2 +/- 0.1 microgram/10(7) complete Freund adjuvant-elicited ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.775-778.1981
更新日期:1981-09-01 00:00:00
abstract::Streptococcus agalactiae (group B Streptococcus or GBS) is a common colonizer of the gastrointestinal and genital tracts and an important cause of invasive infections in newborn infants and in adults with predisposing chronic conditions or advanced age. Attachment to epithelial surfaces at mucosal sites is a critical ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00699-12
更新日期:2012-11-01 00:00:00
abstract::At least 10 of 11 demonstrable antigens in unheated culture filtrates of 12 vaccine strains of BCG were shared as determined in a reference antigen-antibody immunoelectrophoresis system. Filtrates from each of the BCG strains gave equivalent skin test reactions in homologously and heterologously sensitized guinea pigs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.5.777-780.1973
更新日期:1973-05-01 00:00:00
abstract::Kinetoplastid membrane protein 11 (KMP-11) from Leishmania donovani is an abundant 11-kDa surface membrane glycoprotein. Lymph node cells from mice of six different H-2 haplotypes immunized with KMP-11 or with L. donovani promastigotes were stimulated to proliferate in vitro KMP-11. Primed purified T cells required an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4893-4899.1994
更新日期:1994-11-01 00:00:00
abstract::Hemolytic-uremic syndrome (HUS) results from infection by Shiga toxin (Stx)-producing Escherichia coli and is the most common cause of acute renal failure in children. We have developed a mouse model of HUS by administering endotoxin-free Stx2 in multiple doses over 7 to 8 days. At sacrifice, moribund animals demonstr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00592-08
更新日期:2008-10-01 00:00:00
abstract::Intra-abdominal polymicrobial infections cause significant morbidity and mortality. An experimental mouse model of Candida albicans-Staphylococcus aureus intra-abdominal infection (IAI) results in 100% mortality by 48 to 72 h postinoculation, while monomicrobial infections are avirulent. Mortality is associated with r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-15
更新日期:2015-10-19 00:00:00
abstract::Thirteen Habs-serotype strains could be classified into 11 groups depending on the characteristic patterns of 2-amino sugar composition; strains of serotypes 2, 5, and 7 had the same pattern, and each other serotype strain had its own distinctive pattern. In our classification, fucosamine and quinovosamine were of imp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.692-697.1977
更新日期:1977-03-01 00:00:00
abstract::Evidence showing that neutrophils play a protective role in the host response to infection by different intracellular parasites has been published in the past few years. We assessed this issue with regard to the infection of mice with Mycobacterium tuberculosis. We found a chronic recruitment of neutrophils to the inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.577-583.2000
更新日期:2000-02-01 00:00:00
abstract::The cytopathic effect (CPE) of Escherichia coli producing cytotoxic necrotizing factor type 1 (CNF1) was investigated by using a human epithelial cell (HeLa) model of infection with CNF1-producing E. coli BM2-1. This strain was shown to bind loosely, but massively, to HeLa cells. A 4-h interaction between bacteria and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1694-1705.1996
更新日期:1996-05-01 00:00:00
abstract::This study was undertaken to ascertain the relationship between complement-derived chemotactic factors and complement component 1 inactivator (C1INA) enhancement of neutrophil chemotaxis. Studies were also designed to determine whether the C1s- reactive or binding site on C1INA was functional in altering chemotactic r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::The innate and the adaptive arms of the mucosal immune system must be coordinated to facilitate the control of pathogenic invasion while maintaining immune homeostasis. Toll-like receptors, able to activate the cell to produce bactericidal and inflammatory cytokines but also able to upregulate antigen (Ag)-presenting ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00100-07
更新日期:2008-02-01 00:00:00
abstract::Twenty monoclonal antibodies have been prepared to the erythrocytes from CBA/Ca mice infected with the rodent malaria Plasmodium chabaudi. By immunofluorescence, 15 of these antibodies recognized parasite antigens expressed only during the development of mature trophozoites to schizonts and merozoites, 2 recognized pa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.94-102.1982
更新日期:1982-10-01 00:00:00
abstract::Previous studies have suggested that the Pseudomonas aeruginosa PAK pilus adhesin moiety resides in an epithelial cell-binding domain located in the C-terminal region of the PAK pilin structural protein. Synthetic peptides Ac17red (a synthetic peptide with a sequence identical to that of PAK pilin residues 128 to 144,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3720-3726.1989
更新日期:1989-12-01 00:00:00
abstract::The pH 6 antigen (Psa) of Yersinia pestis consists of fimbriae with adhesive properties of potential importance for the pathogenesis of plague, including pneumonic plague. The Psa fimbriae mediate bacterial binding to human alveolar epithelial cells. The Psa fimbriae bound mostly to one component present in the total ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01153-06
更新日期:2007-03-01 00:00:00
abstract::Mice immunized subcutaneously with heat-killed Escherichia coli were protected from pyelonephritis produced by the intravenous route, but there was little or no protection from ascending infection. No significant protection from ascending or hematogenous pyelonephritis was demonstrated when immunization was accomplish...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.330-334.1972
更新日期:1972-09-01 00:00:00
abstract::Bacterial infection with group B Streptococcus (GBS) represents a prominent threat to neonates and fetuses in the Western world, causing severe organ damage and even death. To improve current therapeutic strategies and to investigate new approaches, an appropriate in vivo model to study the immune response of a human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01235-12
更新日期:2013-05-01 00:00:00
abstract::Most Helicobacter pylori strains express the BabA adhesin, which binds to ABO/Leb blood group antigens on gastric mucin and epithelial cells and is found more commonly in strains that cause peptic ulcers or gastric cancer, rather than asymptomatic infection. We and others have previously reported that in mice, gerbils...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-17
更新日期:2017-05-23 00:00:00