Histoplasma capsulatum fails to trigger release of superoxide from macrophages.


:The yeast form of the dimorphic fungus Histoplasma capsulatum survives within macrophages after phagocytosis. To do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. Using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form of various H. capsulatum strains. Doses of zymosan as low as 20 particles per macrophage elicited superoxide, whereas H. capsulatum failed to induce superoxide even at 160 yeast cells per macrophage. This phenomenon was observed with two virulent strains of H. capsulatum (G217B and G186A) and with an avirulent variant of G186A. Over a 15- to 150-min observation period, zymosan stimulated increasing reduction of ferricytochrome c, but H. capsulatum did not. When added concurrently with zymosan, H. capsulatum had no effect on superoxide production. Therefore, H. capsulatum was unable either to inactivate the oxygen radical or inhibit host cell superoxide response to other competent stimuli. Enzymatically generated superoxide reduced ferricytochrome c even in the presence of H. capsulatum, again implying that the organism does not readily inactivate superoxide. This experiment also demonstrated that the yeast did not interfere with the assay used. Thus, rather than inhibiting superoxide generation or inactivating the anion, H. capsulatum yeast cells appear to avoid the toxic effects of superoxide by failing to trigger its release.


Infect Immun


Infection and immunity


Eissenberg LG,Goldman WE




Has Abstract


1987-01-01 00:00:00












  • Two-dimensional gel electrophoresis and immunoblotting of Campylobacter outer membrane proteins.

    abstract::We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flage...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Dunn BE,Blaser MJ,Snyder EL

    更新日期:1987-07-01 00:00:00

  • Chemical and biological properties of a peptidoglycan isolated from Treponema pallidum kazan.

    abstract::A peptidoglycan layer of Treponema pallidum kazan was isolated by solubilization of whole cells with 1% warm sodium dodecyl sulfate and subsequent digestion of an insoluble residue with proteases. Electron microscopy revealed that the peptidoglycan was isolated as a single-layered sacculus of less than 5 nm in thickne...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Umemoto T,Ota T,Sagawa H,Kato K,Takada H,Tsujimoto M,Kawasaki A,Ogawa T,Harada K,Kotani S

    更新日期:1981-02-01 00:00:00

  • Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model.

    abstract::Wild-type isolates of Shigella flexneri bind the dye Congo red from solid media, thus producing red (Crb+) colonies. Mutants which fail to bind the dye produce white colonies (Crb-) and are avirulent in a variety of systems. In S. flexneri the ability to bind Congo red correlates with the ability to bind hemin and pro...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Daskaleros PA,Payne SM

    更新日期:1987-06-01 00:00:00

  • A fadD mutant of Vibrio cholerae is impaired in the production of virulence factors and membrane localization of the virulence regulatory protein TcpP.

    abstract::In the enteric pathogen Vibrio cholerae, expression of the major virulence factors is controlled by the hierarchical expression of several regulatory proteins comprising the ToxR regulon. In this study, we demonstrate that disruption of the fadD gene encoding a long-chain fatty acyl coenzyme A ligase has marked effect...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ray S,Chatterjee E,Chatterjee A,Paul K,Chowdhury R

    更新日期:2011-01-01 00:00:00

  • A recombinant Rickettsia conorii vaccine protects guinea pigs from experimental boutonneuse fever and Rocky Mountain spotted fever.

    abstract::There are no vaccines against boutonneuse fever and Rocky Mountain spotted fever. Previous studies have identified a Rickettsia rickettsii surface protein as a vaccine candidate and shown that an antigenically related protein is present in R. conorii, which causes boutonneuse fever. The gene encoding the R. rickettsii...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Vishwanath S,McDonald GA,Watkins NG

    更新日期:1990-03-01 00:00:00

  • Cinematographic observations of growth cycles of Chlamydia trachomatis in primary cultures of human amniotic cells.

    abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Neeper ID,Patton DL,Kuo CC

    更新日期:1990-07-01 00:00:00

  • Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection.

    abstract::Cutaneous leishmaniasis (CL) caused by Leishmania braziliensis is characterized by a strong Th1 response that leads to skin lesion development. In areas where L. braziliensis transmission is endemic, up to 15% of healthy subjects have tested positive for delayed-type hypersensitivity to soluble leishmania antigen (SLA...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cardoso TM,Machado Á,Costa DL,Carvalho LP,Queiroz A,Machado P,Scott P,Carvalho EM,Bacellar O

    更新日期:2015-03-01 00:00:00

  • Detection in antisera of antibodies that cross-react with herpes simplex virus type 1 glycoprotein gC.

    abstract::Herpes simplex virus type 1 (HSV-1) glycoprotein gC was purified by affinity chromatography with an immunosorbent column containing monoclonal antibody to HSV-1 gC, and its reactivity with rabbit antisera was measured by enzyme-linked immunosorbent assay and sodium dodecyl sulfate-polyacrylamide gel electrophoretic an...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zweig M,Heilman CJ Jr,Bladen SV,Showalter SD,Hampar B

    更新日期:1983-08-01 00:00:00

  • Identification of an Autorepressing Two-Component Signaling System That Modulates Virulence in Streptococcus suis Serotype 2.

    abstract::Streptococcus suis is one of the most important pathogens affecting the swine industry and is also an emerging zoonotic agent for humans. Two-component signaling systems (TCSs) play important roles in the adaptation of pathogenic bacteria to host environments. In this study, we identified a novel TCS, named TCS09HKRR,...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zhong X,Zhang Y,Zhu Y,Dong W,Ma J,Pan Z,Yao H

    更新日期:2019-08-21 00:00:00

  • Adjuvant activity of naturally occurring monophosphoryl lipopolysaccharide preparations from mucosa-associated bacteria.

    abstract::Natural heterogeneity in the structure of the lipid A portion of lipopolysaccharide (LPS) produces differential effects on the innate immune response. Gram-negative bacterial species produce LPS structures that differ from the classic endotoxic LPS structures. These differences include hypoacylation and hypophosphoryl...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chilton PM,Hadel DM,To TT,Mitchell TC,Darveau RP

    更新日期:2013-09-01 00:00:00

  • Resolution of secondary Chlamydia trachomatis genital tract infection in immune mice with depletion of both CD4+ and CD8+ T cells.

    abstract::The essential role of T cells in the resolution of primary murine Chlamydia trachomatis genital tract infection is inarguable; however, much less is known about the mechanisms that confer resistance to reinfection. We previously established that CD4+ T cells and B cells contribute importantly to resistance to reinfect...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Morrison SG,Morrison RP

    更新日期:2001-04-01 00:00:00

  • The oligomerization domain of C4-binding protein (C4bp) acts as an adjuvant, and the fusion protein comprised of the 19-kilodalton merozoite surface protein 1 fused with the murine C4bp domain protects mice against malaria.

    abstract::Highly purified protein antigens are usually poor immunogens; in practice, adjuvants are needed to obtain satisfactory immune responses. Plasmodium yoelii 19-kDa merozoite surface protein 1 (MSP1(19)) is a weak antigen, but mice vaccinated with this antigen in strong adjuvants can survive an otherwise lethal parasite ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ogun SA,Dumon-Seignovert L,Marchand JB,Holder AA,Hill F

    更新日期:2008-08-01 00:00:00

  • Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis.

    abstract::Using a PCR-based strategy and degenerate oligonucleotides, we isolated a Legionella pneumophila gene that showed high sequence similarity to members of the fliI gene family. An insertion mutation that disrupted the fliI open reading frame was recombined onto the L. pneumophila chromosome and analyzed for its effects ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Merriam JJ,Mathur R,Maxfield-Boumil R,Isberg RR

    更新日期:1997-06-01 00:00:00

  • Immune response to plasmid- and chromosome-encoded Yersinia antigens.

    abstract::The immune response of humans and mice to temperature-specific, plasmid- or chromosome-encoded proteins of yersinia pestis and Yersinia enterocolitica was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. Extracts from Y. pestis and Y. enterocolitica strains with and witho...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mazza G,Karu AE,Kingsbury DT

    更新日期:1985-06-01 00:00:00

  • The large clostridial toxins from Clostridium sordellii and C. difficile repress glucocorticoid receptor activity.

    abstract::We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difficile. This was demonstrated using a transient transfection assay...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tait AS,Dalton M,Geny B,D'Agnillo F,Popoff MR,Sternberg EM

    更新日期:2007-08-01 00:00:00

  • In vitro synthesis of anti-mycobacterial antibodies in biopsies from skin lesions of leprosy patients.

    abstract::To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The re...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lai A Fat RF,Chan Pin Jin J,van Furth R,Harboe M

    更新日期:1980-02-01 00:00:00

  • Monoclonal antibodies reactive with all strains of Haemophilus ducreyi.

    abstract::We have isolated plasma cell hybridomas which secrete monoclonal antibodies directed against Haemophilus ducreyi. Two of these monoclonal antibodies recognize all strains of H. ducreyi tested to date and are capable of detecting the presence of H. ducreyi in skin lesions produced by this pathogen in experimental anima...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hansen EJ,Loftus TA

    更新日期:1984-04-01 00:00:00

  • Strain variation in antiphagocytic activity of capsular polysaccharides from Cryptococcus neoformans serotype A.

    abstract::Strains of Cryptococcus neoformans vary in resistance to phagocytosis in vitro. The binding of isolated capsular polysaccharide (CPS) to a capsule-free mutant of C. neoformans confers resistance to phagocytosis. The importance of capsule composition to differences among strains in susceptibility to phagocytosis was ev...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Small JM,Mitchell TG

    更新日期:1989-12-01 00:00:00

  • Isolation and partial characterization of culture-derived soluble Babesia bovis antigens.

    abstract::Immunochemical analyses of soluble antigens derived from microaerophilous stationary phase cultures of Babesia bovis demonstrated that at least three parasite antigens were released in vitro. These antigens have molecular weights within the range of 37,000 to 40,000, fast electrophoretic mobility in the albumin and al...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: James MA,Levy MG,Ristic M

    更新日期:1981-01-01 00:00:00

  • Trypanosoma rhodesiense infection in congenitally athymic (nude) mice.

    abstract::Athymic nude mice (nu/nu), heterozygous litter mates (nu/+), and thymic reconstituted homozygous animals (nu/nu TxR) were infected with Trypanosoma rhodesiense. A reduced parasitemia and prolonged survival were observed in the nu/nu animals after infection and cure or after immunization with irradiated organisms. Thes...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Campbell GH,Esser KM,Phillips SM

    更新日期:1978-06-01 00:00:00

  • A potassium thiocyanate extract vaccine prepared from Pasteurella multocida 3:A protects rabbits against homologous challenge.

    abstract::Potassium thiocyanate extracts of a virulent Pasteurella multocida 3:A rabbit isolate were prepared and used as a vaccine in rabbits. The extract contained protein, carbohydrate, hyaluronic acid, lipopolysaccharide, DNA, and RNA. The protein and lipopolysaccharide profiles of the extract were similar to those of the P...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lu YS,Pakes SP,Massey L,Stefanu C

    更新日期:1987-12-01 00:00:00

  • Role of complement in host defense against pneumococcal otitis media.

    abstract::Strategies to limit complement deposition on Streptococcus pneumoniae are established as virulence features for invasive disease, but their role in respiratory tract infection requires further analysis. We evaluated complement C3 protein deposition on discordant S. pneumoniae isolates of the same serotype (6A) and the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sabharwal V,Ram S,Figueira M,Park IH,Pelton SI

    更新日期:2009-03-01 00:00:00

  • Expression of 65- and 67-kilodalton heat-regulated proteins and a 70-kilodalton heat shock cognate protein of Leishmania donovani in macrophages.

    abstract::Heat shock protein (HSP) expression was examined in murine bone marrow-derived macrophages infected with stationary-phase promastigotes of Leishmania donovani. Immunoblotting performed with a rabbit polyclonal antiserum raised against HSP60 from Heliothis virescens (moth) revealed the de novo appearance of 65- and 67-...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rey-Ladino JA,Reiner NE

    更新日期:1993-08-01 00:00:00

  • Immune response and dental caries incidence in Streptococcus faecalis-monoassociated Harvard caries-resistant and caries-susceptible rats.

    abstract::Harvard caries-resistant rats (CR) monoassociated with cariogenic Streptococcus faecalis (SF) for 150 days developed early high levels of specific SF serum agglutinins, moderate levels of salivary antibody, and little or no caries activity. Harvard caries-susceptible (CS) SF-monoassociated rats developed serum antibod...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Peri BA,Wagner M

    更新日期:1977-06-01 00:00:00

  • Efficacy of enteric-coated protease in preventing attachment of enterotoxigenic Escherichia coli and diarrheal disease in the RITARD model.

    abstract::In this study, we report on a novel approach based on modification of the intestinal surface to prevent diarrhea caused by enterotoxigenic Escherichia coli (ETEC). The removable intestinal tie adult rabbit diarrhea (RITARD) model was used to test the efficacy of an enteric-coated protease preparation (Detach; Enzacor ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mynott TL,Chandler DS,Luke RK

    更新日期:1991-10-01 00:00:00

  • Humoral antibody response against Bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal patients.

    abstract::An antigen recognized by monoclonal antibody specific for Bacteroides gingivalis was purified in the presence of 0.5% (wt/vol) beta-octyl-glucoside by immunoadsorbent column chromatography. The purified antigen was homogeneous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Miyoshi T,Hanazawa S,Hirose K,Saitoh K,Amano S,Ohmori Y,Kitano S

    更新日期:1986-08-01 00:00:00

  • Redox balance via lactate dehydrogenase is important for multiple stress resistance and virulence in Enterococcus faecalis.

    abstract::Enterococcus faecalis is a highly stress resistant opportunistic pathogen. The intrinsic ruggedness of this bacterium is supposed to be the basis of its capacity to colonize the hostile environments of hospitals and to cause several kinds of infections. We show in this work that general resistance to very different en...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rana NF,Sauvageot N,Laplace JM,Bao Y,Nes I,Rincé A,Posteraro B,Sanguinetti M,Hartke A

    更新日期:2013-08-01 00:00:00

  • Bacterial Stimulation of Toll-Like Receptor 4 Drives Macrophages To Hemophagocytose.

    abstract::During acute infection with bacteria, viruses or parasites, a fraction of macrophages engulf large numbers of red and white blood cells, a process called hemophagocytosis. Hemophagocytes persist into the chronic stage of infection and have an anti-inflammatory phenotype. Salmonella enterica serovar Typhimurium infecti...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McDonald EM,Pilonieta MC,Nick HJ,Detweiler CS

    更新日期:2015-10-12 00:00:00

  • Plasmodium chabaudi-infected erythrocytes adhere to CD36 and bind to microvascular endothelial cells in an organ-specific way.

    abstract::Adherence of erythrocytes infected with Plasmodium falciparum to microvascular endothelial cells (sequestration) is considered to play an important role in parasite virulence and pathogenesis. However, the real importance of sequestration for infection and disease has never been fully assessed. The absence of an appro...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mota MM,Jarra W,Hirst E,Patnaik PK,Holder AA

    更新日期:2000-07-01 00:00:00

  • Pathogenesis of rinderpest virus infection in rabbits. II. Effect of rinderpest virus on the immune functions of rabbits.

    abstract::Rinderpest virus infection was shown to induce marked suppression of both humoral antibody response and cell-mediated immunity in rabbits. The virus exhibited a suppressive effect on primary antibody response as indicated by a decrease in numbers of plaque-forming cells (immunoglobulin [Ig]M) and hemagglutinating anti...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yamanouchi K,Fukuda A,Kobune F,Yoshikawa Y,Chino F

    更新日期:1974-02-01 00:00:00