Histoplasma capsulatum fails to trigger release of superoxide from macrophages.


:The yeast form of the dimorphic fungus Histoplasma capsulatum survives within macrophages after phagocytosis. To do so, it must avoid, inhibit, or resist a variety of toxic oxygen metabolites. Using ferricytochrome c reduction to assay superoxide release, we examined the response of mouse macrophages to the yeast form of various H. capsulatum strains. Doses of zymosan as low as 20 particles per macrophage elicited superoxide, whereas H. capsulatum failed to induce superoxide even at 160 yeast cells per macrophage. This phenomenon was observed with two virulent strains of H. capsulatum (G217B and G186A) and with an avirulent variant of G186A. Over a 15- to 150-min observation period, zymosan stimulated increasing reduction of ferricytochrome c, but H. capsulatum did not. When added concurrently with zymosan, H. capsulatum had no effect on superoxide production. Therefore, H. capsulatum was unable either to inactivate the oxygen radical or inhibit host cell superoxide response to other competent stimuli. Enzymatically generated superoxide reduced ferricytochrome c even in the presence of H. capsulatum, again implying that the organism does not readily inactivate superoxide. This experiment also demonstrated that the yeast did not interfere with the assay used. Thus, rather than inhibiting superoxide generation or inactivating the anion, H. capsulatum yeast cells appear to avoid the toxic effects of superoxide by failing to trigger its release.


Infect Immun


Infection and immunity


Eissenberg LG,Goldman WE




Has Abstract


1987-01-01 00:00:00












  • Natural variation within the principal adhesion domain of the Plasmodium vivax duffy binding protein.

    abstract::The blood-stage development of malaria parasites is initiated by the invasion of merozoites into susceptible erythrocytes. Specific receptor-ligand interactions must occur for the merozoites to first attach to and then invade erythrocytes. Because the invasion process is essential for the parasite's survival and the m...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tsuboi T,Kappe SH,al-Yaman F,Prickett MD,Alpers M,Adams JH

    更新日期:1994-12-01 00:00:00

  • Susceptibility to Plasmodium yoelii preerythrocytic infection in BALB/c substrains is determined at the point of hepatocyte invasion.

    abstract::After transmission by Anopheles mosquitoes, Plasmodium sporozoites travel to the liver, infect hepatocytes, and rapidly develop as intrahepatocytic liver stages (LS). Rodent models of malaria exhibit large differences in the magnitude of liver infection, both between parasite species and between strains of mice. This ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kaushansky A,Austin LS,Mikolajczak SA,Lo FY,Miller JL,Douglass AN,Arang N,Vaughan AM,Gardner MJ,Kappe SH

    更新日期:2015-01-01 00:00:00

  • Roles of endogenous gamma interferon and macrophage microbicidal mechanisms in host response to chemotherapy in experimental visceral leishmaniasis.

    abstract::In experimental visceral leishmaniasis, in which the tissue macrophage is the target, in vivo responsiveness to conventional chemotherapy (pentavalent antimony [Sb]) requires a T-cell-dependent mechanism. To determine if this mechanism involves gamma interferon (IFN-gamma)-induced activation and/or specific IFN-gamma-...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Murray HW,Delph-Etienne S

    更新日期:2000-01-01 00:00:00

  • Mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae remains a major causative agent of serious human diseases. The worldwide increase of antibiotic resistant strains revealed the importance of horizontal gene transfer in this pathogen, a scenario that results in the modulation of the species-specific gene pool. We investigated genomic variation...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hakenbeck R,Balmelle N,Weber B,Gardès C,Keck W,de Saizieu A

    更新日期:2001-04-01 00:00:00

  • Immunogenicity and protective immunity induced by synthetic peptides associated with putative immunodominant regions of Streptococcus mutans glucan-binding protein B.

    abstract::Glucan-binding protein B (GbpB) from Streptococcus mutans has been shown to induce protective immunity to dental caries in experimental models. Having recently sequenced the gbpB gene, our objective in this study was to identify immunogenic regions within the GbpB sequence for use in subunit vaccines. Potential region...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Smith DJ,King WF,Barnes LA,Peacock Z,Taubman MA

    更新日期:2003-03-01 00:00:00

  • Comparison of haemophilus influenzae type b interaction with respiratory mucosa organ cultures maintained with an air interface or immersed in medium.

    abstract::Haemophilus influenzae type b infection of immersed and air interface organ cultures for 24 h caused significant epithelial damage. Bacterial association with mucus, damaged epithelium, and unciliated cells was significantly higher in air interface than immersed organ cultures, and total bacterial association was 55.8...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jackson AD,Cole PJ,Wilson R

    更新日期:1996-06-01 00:00:00

  • Characterization of virulence plasmid diversity among Clostridium perfringens type B isolates.

    abstract::The important veterinary pathogen Clostridium perfringens type B is unique for producing the two most lethal C. perfringens toxins, i.e., epsilon-toxin and beta-toxin. Our recent study (K. Miyamoto, J. Li, S. Sayeed, S. Akimoto, and B. A. McClane, J. Bacteriol. 190:7178-7188, 2008) showed that most, if not all, type B...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sayeed S,Li J,McClane BA

    更新日期:2010-01-01 00:00:00

  • Identification of functional domains of Bordetella dermonecrotizing toxin.

    abstract::Bordetella dermonecrotizing toxin (DNT) stimulates the assembly of actin stress fibers and focal adhesions by deamidating Gln63 of the small GTPase Rho. To clarify the functional and structural organization of DNT, we cloned and sequenced the DNT gene and examined the functions of various DNT mutants. Our analyses of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kashimoto T,Katahira J,Cornejo WR,Masuda M,Fukuoh A,Matsuzawa T,Ohnishi T,Horiguchi Y

    更新日期:1999-08-01 00:00:00

  • Gonococcal opacity protein promotes bacterial entry-associated rearrangements of the epithelial cell actin cytoskeleton.

    abstract::Neisseria gonorrhoeae enters cultured human mucosal cells following binding of a distinct gonococcal opacity (Opa) outer membrane protein to cell surface proteoglycan receptors. We examined the route of internalization that is activated by Opa-expressing gonococci (strain VP1). Microscopy of infected Chang epithelial ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Grassmé HU,Ireland RM,van Putten JP

    更新日期:1996-05-01 00:00:00

  • In vitro transformation of mouse bone-marrow-derived (B) lymphocytes induced by the lipid component of endotoxin.

    abstract::An analysis of which component of lipopolysaccharide, the lipid or the polysaccharide, is mitogenic for mouse B-lymphocytes has been performed. A purified glycolipid derived from a rough mutant of Salmonella minnesota (R595) that does not contain any o-polysaccharide at all is more mitogenic than an intact lipopolysac...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rosenstreich DL,Nowotny A,Chused T,Mergenhagen SE

    更新日期:1973-09-01 00:00:00

  • Siderophore production by cystic fibrosis isolates of Burkholderia cepacia.

    abstract::Sixty-one Burkholderia cepacia isolates from patients with cystic fibrosis (CF) and four plant isolates were screened for production of the siderophores salicylic acid (SA), pyochelin, cepabactin, and ornibactins and fingerprinted by a PCR-based randomly amplified polymorphic DNA (RAPD) method. Of the 24 RAPD types de...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Darling P,Chan M,Cox AD,Sokol PA

    更新日期:1998-02-01 00:00:00

  • Bactericidal activity of alveolar and peritoneal macrophages exposed in vitro to three strains of Pasteurella multocida.

    abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Collins FM,Niederbuhl CJ,Campbell SG

    更新日期:1983-02-01 00:00:00

  • Direct activation of human monocyte-derived macrophages by a bacterial glycoprotein extract inhibits the intracellular multiplication of virulent Legionella pneumophila serogroup 1.

    abstract::Intracellular multiplication of virulent Legionella pneumophila serogroup 1 was inhibited by human monocyte-derived macrophages activated by a glycoprotein extract of Klebsiella pneumoniae, RU 41.740. Macrophage cultures were infected with L. pneumophila in the presence of immune antibodies on day 7 of culture. Extrac...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rajagopalan P,Dournon E,Vildé JL,Pocidalo JJ

    更新日期:1987-09-01 00:00:00

  • Intestinal and serum antibody responses in mice after oral immunization with Salmonella, Escherichia coli, and Salmonella-Escherichia coli hybrid strains.

    abstract::After oral feeding of mice with avirulent Salmonella, Escherichia coli, or hybrid strains, only certain bacterial strains were able to multiply and persist within the small intestinal Peyer's patches. After oral vaccination alone, or oral priming and subsequent parenteral boosting, antibody class and titers were detec...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hohmann A,Schmidt G,Rowley D

    更新日期:1979-07-01 00:00:00

  • Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells.

    abstract::We previously reported that infection by Fusobacterium nucleatum strongly induced the expression of both human beta-defensin 2 (HBD-2) and HBD-3 by gingival epithelial cells. The aim of this study was to characterize the pattern recognition receptors (PRRs) and regulatory mechanisms involved in the induction of HBD-2 ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ji S,Shin JE,Kim YS,Oh JE,Min BM,Choi Y

    更新日期:2009-03-01 00:00:00

  • Effect of pH on the production of the Kanagawa hemolysin by Vibrio parahaemolyticus.

    abstract::Production of the Kanagawa hemolysin by patient strains of Vibrio parahaemolyticus was found to respond to the pH rather than to the type of carbohydrate present in the growth medium. Regardless of the carbohydrate present, hemolysin production in peptone broth cultures occurred only when the pH was between 6.5 and 5....

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cherwonogrodzky JW,Clark AG

    更新日期:1981-10-01 00:00:00

  • Endotoxin-induced tumor necrosis factor alpha synthesis in murine embryo fibroblasts.

    abstract::Murine embryo fibroblasts (MEF) were found to secrete tumor necrosis factor (TNF) in response to stimulation with endotoxin. Endotoxin-induced TNF production by MEF was inhibited by cycloheximide. However, reversal of the effect of this inhibitor on protein synthesis results in TNF being secreted in amounts equivalent...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Havell EA,Rogerson BJ

    更新日期:1993-05-01 00:00:00

  • Role of antiviral antibodies in resistance against coxsackievirus B3 infection: interaction between preexisting antibodies and an interferon inducer.

    abstract::An experimental model of coxsackievirus B3 infection in newborn mice was utilized to examine the protective role of antiviral antibodies and an interferon inducer, polyinosinic acid-polycytidylic acid [poly(I:C)]. Subcutaneous administration to the infected mice of specific antiviral antibodies resulted in significant...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cho CT,Feng KK,McCarthy VP,Lenahan MF

    更新日期:1982-08-01 00:00:00

  • Biofilm formation by Moraxella catarrhalis in vitro: roles of the UspA1 adhesin and the Hag hemagglutinin.

    abstract::Mutant analysis was used to identify Moraxella catarrhalis gene products necessary for biofilm development in a crystal violet-based assay involving 24-well tissue culture plates. The wild-type M. catarrhalis strains that formed the most extensive biofilms in this system proved to be refractory to transposon mutagenes...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Pearson MM,Laurence CA,Guinn SE,Hansen EJ

    更新日期:2006-03-01 00:00:00

  • Cloning of Syrian hamster (Mesocricetus auratus) cytokine cDNAs and analysis of cytokine mRNA expression in experimental visceral leishmaniasis.

    abstract::The Syrian golden hamster (Mesocricetus auratus) is uniquely susceptible to a variety of intracellular pathogens and is an excellent model for a number of human infectious diseases. The molecular basis for this high level of susceptibility is unknown, and immunological studies related to this model have been limited b...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Melby PC,Tryon VV,Chandrasekar B,Freeman GL

    更新日期:1998-05-01 00:00:00

  • Experimental scrub typhus immunogens: gamma-irradiated and formalinized rickettsiae.

    abstract::Scrub typhus immunogens were prepared by exposing infected yolk sac suspensions of Rickettsia tsutsugamushi to various doses of gamma radiation. Mouse lethality was abolished at doses greater than 200 krads, whereas immunogenicity of the suspensions, as shown by mouse protection tests, was diminished relatively little...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Eisenberg GH Jr,Osterman JV

    更新日期:1977-01-01 00:00:00

  • New fimbrial gene cluster of S-fimbrial adhesin family.

    abstract::Fimbrial adhesins that mediate attachment to host cells are produced by most virulent Escherichia coli isolates. These virulence factors play an important role in the initial stages of bacterial colonization and also in determination of the host and tissue specificity. Isolates belonging to serotype O78 are known to c...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Babai R,Stern BE,Hacker J,Ron EZ

    更新日期:2000-10-01 00:00:00

  • Inactivation of suppressor T-cell activity by nontoxic monophosphoryl lipid A.

    abstract::Treatment with nontoxic monophosphoryl lipid A (MPL), which was derived from a polysaccharide-deficient, heptoseless Re mutant of Salmonella typhimurium, was found to inactivate suppressor T-cell activity, as evidenced by a decrease in the degree of low-dose immunological paralysis expressed and an increase in the mag...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Baker PJ,Hiernaux JR,Fauntleroy MB,Prescott B,Cantrell JL,Rudbach JA

    更新日期:1988-05-01 00:00:00

  • Characterization of 20K fimbria, a new adhesin of septicemic and diarrhea-associated Escherichia coli strains, that belongs to a family of adhesins with N-acetyl-D-glucosamine recognition.

    abstract::Bovine septicemic Escherichia coli 31A agglutinates bovine, rabbit, and human erythrocytes and adheres in vitro to the brush border of bovine or ovine intestinal epithelial cells and to the human colon carcinoma Caco-2 cell line. The adhesion and hemagglutination of E. coli 31A are mediated by a chromosome-encoded fim...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bertin Y,Girardeau JP,Darfeuille-Michaud A,Contrepois M

    更新日期:1996-01-01 00:00:00

  • Combined effects of blood and temperature shift on Borrelia burgdorferi gene expression as determined by whole genome DNA array.

    abstract::Borrelia burgdorferi undergoes differential gene expression during transmission from its tick vector to a vertebrate host. The addition of blood to a spirochete culture at 35 degrees C for 48 h had a dramatic effect on gene expression of this organism. Utilizing B. burgdorferi whole genome DNA arrays, we compared the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tokarz R,Anderton JM,Katona LI,Benach JL

    更新日期:2004-09-01 00:00:00

  • Targeting improves the efficacy of a DNA vaccine against Corynebacterium pseudotuberculosis in sheep.

    abstract::A large-scale DNA vaccination trial was performed with sheep to investigate whether an antigen targeted by CTLA-4 enhanced and accelerated the humoral immune response. Vaccination with genetically detoxified phospholipase D (DeltaPLD) has been shown to be effective, at least partially, against Corynebacterium pseudotu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chaplin PJ,De Rose R,Boyle JS,McWaters P,Kelly J,Tennent JM,Lew AM,Scheerlinck JP

    更新日期:1999-12-01 00:00:00

  • Factors affecting filamentation in Candida albicans: changes in respiratory activity of Candida albicans during filamentation.

    abstract::Glucose metabolism and respiration of Candida albicans were compared under conditions which permitted either maximal filamentous or maximal yeast growth. Changes in metabolism were monitored by comparing the quantities of ethanol produced, CO2 evolved, and oxygen consumed. Filamenting cultures produced more ethanol an...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Land GA,McDonald WC,Stjernholm RL,Friedman L

    更新日期:1975-07-01 00:00:00

  • Replication of vesicular stomatitis virus in murine spleen cells: enrichment of the virus-replicating lymphocytes and analysis of replication restriction.

    abstract::Vesicular stomatitis virus (Indiana strain) will only grow in T lymphocytes which have been stimulated to undergo cell division. Evidence is presented that a considerable enrichment of the vesicular stomatitis virus-replicating T cells may be accomplished in the mouse spleen by passing the spleen cells over glass wool...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Webb DR,Munshi S,Banerjee AK

    更新日期:1981-04-01 00:00:00

  • The sigma B regulon influences internalization of Staphylococcus aureus by osteoblasts.

    abstract::Individual strains of Staphylococcus aureus have different capacities to become internalized by osteoblasts. Here we report that the levels of sigma(B) expressed by S. aureus correlate with the capacity of this bacterium to be internalized by osteoblasts. However, sigma(B) is not essential for internalization and does...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nair SP,Bischoff M,Senn MM,Berger-Bächi B

    更新日期:2003-07-01 00:00:00

  • Innate resistance of mice to experimental infection with Naegleria fowleri.

    abstract::The mouse system provides an excellent model for studying host resistance to Naegleria fowleri, the agent of primary amoebic meningoencephalitis. Innate resistance to infection with N. fowleri was examined with respect to infecting dose and the age, sex, and strain of mice. Intravenous inoculation with 10(7) amoebae p...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Haggerty RM,John DT

    更新日期:1978-04-01 00:00:00