Abstract:
:The method of hemagglutination inhibition was used to investigate the antigenic diversity of lipopolysaccharide (LPS) from Neisseria meningitidis and to develop a serotyping systems based on this antigen. The system uses outer membrane complex prepared by a simple extraction procedure to inhibit homologous hemagglutination reactions involving sheep erythrocytes sensitized with purified LPS and rabbit antiserum raised to whole meningococci. Antisera with specificity for eight different LPS determinants were used as typing sera to serotype a cross section of 67 meningococcal strains. Only two strains (both group A) were not typable with the eight sera, and most strains had more than one type. Comparison of LPS type and bactericidal serotype suggests that the LPS and protein serotypes are independent serological markers.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Mandrell RE,Zollinger WDdoi
10.1128/IAI.16.2.471-475.1977subject
Has Abstractpub_date
1977-05-01 00:00:00pages
471-5issue
2eissn
0019-9567issn
1098-5522journal_volume
16pub_type
杂志文章abstract::Mapping vectors containing antibiotic resistance markers inserted adjacent to or within different cloned genes from Streptococcus mutans were used to determine the relative positions of these genes on the chromosome. The gtfA, ftf, and scrB genes were inserted into streptococcal mapping vector pVA891 adjacent to an Em...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.805-809.1989
更新日期:1989-03-01 00:00:00
abstract::Peptidoglycan from a Staphylococcus epidermidis strain, isolated from a patient with septicemia, was preincubated with human serum. This mixture was then investigated for its potency to induce tumor necrosis factor (TNF) secretion by human blood monocytes. TNF was measured in the supernatants by using a bioassay and/o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3837-3843.1994
更新日期:1994-09-01 00:00:00
abstract::The production of interleukin-6 (IL-6) and its possible relationship to host resistance and inflammatory response to Pneumocystis carinii infection were examined in mice with severe combined immunodeficiency (SCID mice). IL-6 activity was detected in the serum and lungs of P. carinii-infected mice but not in mice free...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.97-102.1993
更新日期:1993-01-01 00:00:00
abstract::Although there appears to be little if any role for specific antibodies in protection against intracellular bacteria, such as the model pathogen F. tularensis live vaccine strain (LVS), the role of B cells themselves in primary and secondary infection with such bacteria has not been examined directly. We show here tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6002-6007.1999
更新日期:1999-11-01 00:00:00
abstract::Subunits of a proline-rich coccidioidal antigen (Ag2/PRA) of Coccidioides immitis were analyzed by comparison as vaccines in mice. The optimal dose of plasmid vaccine encoding full-length Ag2/PRA was determined to be between 10 and 100 microg. Mice vaccinated with plasmids encoding amino acids (aa) 1 to 106 were as pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3330-3335.2002
更新日期:2002-07-01 00:00:00
abstract::A total of five bacteriological samples from the periodontal pockets of two sisters with localized juvenile periodontitis and their mother with advanced periodontitis was studied. Gram-negative anaerobic rods were predominant in the samples. Bacteroides intermedius and Bacteroides loescheii were the most predominant s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.118-121.1984
更新日期:1984-07-01 00:00:00
abstract::The levels of protection found in vaccine studies of murine visceral leishmaniasis are significantly lower than for cutaneous leishmaniasis; whether this is due to the high-challenge murine model employed and/or is a consequence of differences required in tissue-specific local immune responses is not understood. Conse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.401-410.2003
更新日期:2003-01-01 00:00:00
abstract::Twenty monoclonal antibodies have been prepared to the erythrocytes from CBA/Ca mice infected with the rodent malaria Plasmodium chabaudi. By immunofluorescence, 15 of these antibodies recognized parasite antigens expressed only during the development of mature trophozoites to schizonts and merozoites, 2 recognized pa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.94-102.1982
更新日期:1982-10-01 00:00:00
abstract::Borrelia burgdorferi resembles gram-negative bacteria in having both cellular and outer membranes. We previously showed that a lipopolysaccharide (LPS)-like material could be extracted from B. burgdorferi with phenol-chloroform-petroleum ether (PCP). The PCP extract of B. burgdorferi exhibited biological activity in s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.4995-5003.1992
更新日期:1992-12-01 00:00:00
abstract::The gene expression and cytokine release of the proinflammatory cytokines interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) after infection of human epithelial cells (HEp-2 cells) and polymorphonuclear granulocytes (PMNs) were investigated by using isogenic pairs of Listeria monocytogen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2545-2552.1993
更新日期:1993-06-01 00:00:00
abstract::Protective immunity against pulmonary tuberculosis (TB) is characterized by the formation in the lungs of granulomas consisting of macrophages and activated T cells producing tumor necrosis factor alpha and gamma interferon, both required for the activation of the phagocytes. In 90% of immunocompetent humans, this res...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7099-7108.2003
更新日期:2003-12-01 00:00:00
abstract::By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi geno...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2047-2053.1988
更新日期:1988-08-01 00:00:00
abstract::Escherichia coli containing the K1 capsule is the leading cause of gram-negative meningitis, but the pathogenesis of this disease is not completely understood. Recent microarray experiments in which we compared the gene expression profile of E. coli K1 associated with human brain microvascular endothelial cells (HBMEC...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01543-06
更新日期:2007-06-01 00:00:00
abstract::Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05048-11
更新日期:2011-09-01 00:00:00
abstract::Autoclaving cell wall of Streptococcus mutans Ingbritt for 15 min under the Rantz and Randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. The extract contained the serotype c-specific antigen but lacked the lipoteichoic acid compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.842-851.1978
更新日期:1978-12-01 00:00:00
abstract::In contrast to infection of superficial tissues, Streptococcus pyogenes infection of deeper tissue can be associated with a significantly diminished inflammatory response, suggesting that this bacterium has the ability to both promote and suppress inflammation. To examine this, we analyzed the behavior of an S. pyogen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00420-09
更新日期:2009-11-01 00:00:00
abstract::Burkholderia pseudomallei is a CDC tier 1 select agent that causes melioidosis, a severe disease in humans and animals. Persistent infections are common, and there is currently no vaccine available. Lipopolysaccharide (LPS) is a potential vaccine candidate. B. pseudomallei expresses three serologically distinct LPS ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02785-14
更新日期:2015-05-01 00:00:00
abstract::T cells are important in systemic anticryptococcal defenses, but a role in controlling an initial pulmonary infection has not been demonstrated. A murine model with intratracheal inoculation was developed to study the acquisition and expression of pulmonary T cell-mediated immunity against Cryptococcus neoformans. Inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1423-1433.1991
更新日期:1991-04-01 00:00:00
abstract::Once in the cytoplasm of mammalian cells, Shigella flexneri expresses a motile phenotype caused by polar directional assembly of actin. This process depends on accumulation of IcsA (VirG), a 120-kDa protein with ATPase activity, at the pole of the bacterium opposite to that at which ongoing septation occurs. IcsA is a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.511-517.1996
更新日期:1996-02-01 00:00:00
abstract::Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. To circumvent this problem, we injected CpG DNA ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00700-09
更新日期:2009-12-01 00:00:00
abstract::Haemophilus influenzae is a commensal and opportunistic pathogen of the human airways. A number of surface molecules contribute to colonization of the airways by H. influenzae, such as adhesins, including structures found in the lipooligosaccharide (LOS). A human bronchiolar xenograft model was employed to investigate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4661-4668.2002
更新日期:2002-08-01 00:00:00
abstract::Hamster (Mesocricetus auratus) neutrophil granules contain at least four microbicidal peptides belonging to the defensin family. These compounds were purified from granule acid extracts by reverse-phase chromatography and termed HaNP-1 to -4 (hamster neutrophil peptide). HaNP-1 and HaNP-3 revealed the most bactericida...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4444-4449.1996
更新日期:1996-11-01 00:00:00
abstract::Bovine mastitis continues to be the most detrimental factor for profitable dairying. Recent research conducted within our laboratory has identified a genetic marker in the CXCR2 gene associated with mastitis susceptibility. The objective of the present study was to evaluate the migratory ability of neutrophils from co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4955-4959.2005
更新日期:2005-08-01 00:00:00
abstract::Experimental infections of mice with the African trypanosome Trypanosoma brucei lead to a profound state of T-cell unresponsiveness in the lymph node cell (LNC) compartment. This suppression is mediated by macrophage-like cells which inhibit interleukin 2 (IL-2) secretion and down-regulate IL-2 receptor expression (M....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.1937-1943.1996
更新日期:1996-06-01 00:00:00
abstract::The mouse system provides an excellent model for studying host resistance to Naegleria fowleri, the agent of primary amoebic meningoencephalitis. Innate resistance to infection with N. fowleri was examined with respect to infecting dose and the age, sex, and strain of mice. Intravenous inoculation with 10(7) amoebae p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.73-77.1978
更新日期:1978-04-01 00:00:00
abstract::Filarial infection is initiated by mosquito-derived third-stage larvae (L3) deposited on the skin that transit through the epidermis, which contains Langerhans cells (LC) and keratinocytes (KC), among other cells. This earliest interaction between L3 and the LC likely conditions the priming of the immune system to the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01301-12
更新日期:2013-05-01 00:00:00
abstract::The yields of exotoxin A in Pseudomonas aeruginosa cultures were influenced by the concentration of iron in the culture media. When the iron concentration of the culture media was increased from 0.05 to 1.5 microgram/ml, there was at least a 90% decrease in exotoxin A (measured both by enzymatic activity and by mouse ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.785-791.1978
更新日期:1978-03-01 00:00:00
abstract::Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1420-1425.1996
更新日期:1996-04-01 00:00:00
abstract::The generation of leukotrienes (LTC4, LTD4, LTE4, and LTB4; 12-epi-LTB4 isomer) from human granulocytes by thiol-activated toxins (streptolysin O, alveolysin from Bacillus alvei, and theta toxin from Clostridium perfringens) is described. The release occurs under noncytolytic conditions. Although LTB4 is the major com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.844-851.1985
更新日期:1985-12-01 00:00:00
abstract::Monoclonal antibodies (MAbs) were raised against native and denatured alpha-hemolysin (HlyA) of Escherichia coli. Binding of the MAbs to native, denatured, and erythrocyte-complexed active wild-type hemolysin and mutant derivatives was tested. All 15 MAbs analyzed bound to native hemolysin, even when the toxin was com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.5.1846-1852.1991
更新日期:1991-05-01 00:00:00