Abstract:
:The gene expression and cytokine release of the proinflammatory cytokines interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) after infection of human epithelial cells (HEp-2 cells) and polymorphonuclear granulocytes (PMNs) were investigated by using isogenic pairs of Listeria monocytogenes and Yersinia enterocolitica strains. By polymerase chain reaction-assisted mRNA amplification and RNA dot blot analysis, we showed that PMNs and HEp-2 cells expressed enhanced levels of mRNA encoding IL-1 beta, IL-6, and TNF-alpha after bacterial infection. Concomitant with the enhanced mRNA level, an increased secretion rate of IL-1 beta, IL-6, and TNF-alpha from PMNs as assessed by enzyme-linked immunosorbent assay was observed. HEp-2 cells after infection also released IL-6 and TNF-alpha into the cell supernatant, while no IL-1 beta release was detected. Cellular coincubation experiments were carried out with Transwell chambers. Our studies revealed that the coculture of PMNs and HEp-2 cells led to an increased IL-1 beta and IL-6 release. In contrast, after infection with the invasive bacteria, reduced levels of TNF-alpha were measured. Our data show that PMNs secrete the proinflammatory cytokines IL-1 beta, IL-6, and TNF-alpha within some hours after infection with L. monocytogenes and Y. enterocolitica and that cellular interactions with epithelial cells alone via soluble mediators influence the net amount of released proinflammatory cytokines.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Arnold R,Scheffer J,König B,König Wdoi
10.1128/IAI.61.6.2545-2552.1993subject
Has Abstractpub_date
1993-06-01 00:00:00pages
2545-52issue
6eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Binding of the complement-downregulating protein factor H (fH) to the surface of the meningococcus is important for survival of the organism in human serum. The meningococcal vaccine candidate factor H binding protein (fHbp) is an important ligand for human fH. While some fHbp-specific monoclonal antibodies (MAbs) blo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05182-11
更新日期:2011-09-01 00:00:00
abstract::Specific pathogen-free guinea pigs were infected via the respiratory route with viable, attenuated Mycobacterium tuberculosis H37Ra and maintained on purified isocaloric diets. The control diet contained 30% protein (ovalbumin) and 50 ppm of added zinc (50 micrograms/g), the low protein diet contained 10% protein and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.793-799.1983
更新日期:1983-02-01 00:00:00
abstract::The Salmonella dublin virulence plasmid mediates systemic infection in mice and cattle. Here, we analyze the interaction between wild-type and plasmid-cured Salmonella strains with phagocytes in vitro and in vivo. The intracellular recovery of S. dublin from murine peritoneal and bovine alveolar macrophages cultured i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3385-3393.1996
更新日期:1996-08-01 00:00:00
abstract::Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of bacterial lipopolysaccharide (LPS) and lipoprotein, respectively, were compared for their mitogenic activities on splenocytes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1382-1384.1988
更新日期:1988-05-01 00:00:00
abstract::Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, has been shown to inhibit phagocytosis of both itself and secondary targets in vitro. Immunodepletion of LspA proteins from H. ducreyi culture supernatant fluid abolished this inhibitory effect, indicating that the LspA proteins ar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.7808-7816.2005
更新日期:2005-12-01 00:00:00
abstract::One- to three-day-old guinea pigs were inoculated intranasally with the chlamydial agent of guinea pig inclusion conjunctivitis. Physical signs of infection included a marked increase in respiration rate on days 5 to 10 of infection and radiographic evidence of pneumonia on day 6. When animals were killed at various t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.153-158.1985
更新日期:1985-04-01 00:00:00
abstract::Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1391-1396.1974
更新日期:1974-12-01 00:00:00
abstract::To determine the ultrastructural distribution and immunological accessibility of proteins II (P.IIs) on the surfaces of whole gonococci, anti-P.II gold probes were developed and used in electron microscopic studies of viable P.II-expressing variants of Neisseria gonorrhoeae FA1090. Anti-P.II probes clearly marked the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.1003-1006.1988
更新日期:1988-04-01 00:00:00
abstract::Lipopolysaccharide (LPS) structure impacts the bactericidal action of cationic peptides, such as polymyxin B (PMB), and sensitivity to killing by normal human serum (NHS). Cultivation of different subspecies strains of Yersinia pestis isolated from unrelated geographic origins at various temperatures (mammals, 37 degr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7324-7331.2005
更新日期:2005-11-01 00:00:00
abstract::The rfb region from Vibrio cholerae O139 strain MO45 was cloned from cosmid gene banks established in Escherichia coli HB101, using an immunoblot assay for screening of the correct clones. Immunoblot analysis of lipopolysaccharide (LPS) preparations revealed the presence of two types of positive clones: (i) those expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3565-3570.1996
更新日期:1996-09-01 00:00:00
abstract::It has been shown previously that serum from chronically infected patients with cystic fibrosis inhibits the phagocytosis of Pseudomonas aeruginosa by both normal and cystic fibrosis alveolar macrophages. In the present study, the ability of peripheral monocytes and polymorphonuclear leukocytes from normal volunteers ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.802-805.1982
更新日期:1982-11-01 00:00:00
abstract::Vitamin E protects nonimmunized and immunized mice against fatal Diplococcus pneumoniae type I (DpI) infection. A dietary supplementation of 180 mg of dl-alpha-tocopheryl acetate per kg of diet increased survival of nonimmunized mice from 20 to 80% when challenged with 20 organisms, and of mice immunized with 0.5 ng o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1292-1295.1974
更新日期:1974-12-01 00:00:00
abstract::The cytoskeletal lesions associated with enteropathogenic Escherichia coli adhering to cultured HeLa epithelial cells were examined by immunofluorescence microscopy. The microfilament-associated proteins actin, alpha-actinin, talin, and ezrin were localized with adherent enteropathogenic E. coli, whereas tropomyosin, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2541-2543.1992
更新日期:1992-06-01 00:00:00
abstract::Protective immunity against tuberculosis is considered to be essentially cell mediated, and an important role for CD8(+) T lymphocytes has been suggested by several studies of murine and human infections. The present work, using an experimental model of infection with Mycobacterium bovis in cattle, showed that live M....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1034-1044.1999
更新日期:1999-03-01 00:00:00
abstract::Dialyzable Lawrence-type transfer factor was prepared from the spleen cells of CF1 mice inoculated with Coccidioides immitis- and Candida albicans-killed vaccines and with live Mycobacterium tuberculosis vaccine (BCG). These preparations were shown to transfer antigen-specific cell-mediated immunity to naive mice, as ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.258-262.1977
更新日期:1977-04-01 00:00:00
abstract::Type II heat-labile enterotoxin (LT-II) from Escherichia coli 41 was purified and compared with prototype LT-II encoded by genes from E. coli SA53. Both toxins were oligomeric proteins consisting of polypeptides A (Mr, 28,000) and B (Mr, 11,800). The A polypeptides were cleaved by trypsin into fragments A1 (Mr, 21,000...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.529-536.1986
更新日期:1986-11-01 00:00:00
abstract::Infections caused by organisms of the Mycobacterium avium complex occur in approximately 50 to 60% of patients with AIDS. M. avium is an intracellular pathogen that survives and multiplies within mononuclear phagocytes. In this study, we investigated the uptake of M. avium grown within macrophages (intracellular growt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1916-1925.1997
更新日期:1997-05-01 00:00:00
abstract::Immunologically competent mice and mice with defined immunological deficiencies were infected with Plasmodium yoelii. Splenomegaly, enhanced phagocytosis, and anemia were most marked in infected mice having intact thymic tissue. Whereas the spleens of infected nude mice increased minimally in size, the relative blood ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.728-731.1978
更新日期:1978-06-01 00:00:00
abstract::The surface antigen makeup of metacyclic trypomastigote forms of strain G of Trypanosoma cruzi, which produce a subpatent infection in mice, differed from those of the virulent strains Y and CL. A 100,000-molecular-weight protein, barely detectable on the Y or CL cell surface, appeared as the main surface antigen of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.836-839.1983
更新日期:1983-05-01 00:00:00
abstract::The cellular source of interleukin-6 (IL-6) during infection of mice with Listeria monocytogenes was investigated both in vitro and in vivo. Peritoneal cells taken at intervals from infected mice and cultured in vitro without added stimulus produced high titers of IL-6 peaking 2 days postinfection in a time course sim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2626-2631.1993
更新日期:1993-06-01 00:00:00
abstract::The within-host dynamics of an infection with the malaria parasite Plasmodium falciparum are the result of a complex interplay between the host immune system and parasite. Continual variation of the P. falciparum erythrocyte membrane protein (PfEMP1) antigens displayed on the surface of infected red blood cells enable...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00958-13
更新日期:2014-01-01 00:00:00
abstract::Cells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucella-specific antibodies. To determine the effect of antibody opsonization on the ability of Brucella to establi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3702-3713.2005
更新日期:2005-06-01 00:00:00
abstract::Antisera were raised in rabbits with acid-treated Re mutant bacteria from Salmonella minnesota and Escherichia coli and tested in a passive hemolysis assay with di- and monophosphorylated free lipid A of E. coli (LipA-Ac and LipA-HCl, respectively) coated onto sheep erythrocytes. Depending on the acid used to prepare ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.776-781.1985
更新日期:1985-06-01 00:00:00
abstract::In the present study, the immunodepressive effects of canine distemper virus (CDV) infection of dogs on two parameters of lymphocyte function, namely phytomitogen-induced cellular proliferation and skin allograft rejection, were investigated. Infection of susceptible gnotobiotic dogs with virulent R252-CDV resulted in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1069-1078.1975
更新日期:1975-05-01 00:00:00
abstract::Some clinical strains of Vibrio cholerae non-O1 produce an extracellular factor that evokes a rapid and dramatic cytotoxic response which manifests as cell rounding of Chinese hamster ovary (CHO) and HeLa cells without accompanying membrane damage. This study was performed to establish the identity of the non-membrane...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3101-3108.1996
更新日期:1996-08-01 00:00:00
abstract::Vaccination has been proposed for the prevention of disease due to enterohemorrhagic Escherichia coli (EHEC), but the immune response following human infection, including the choice of potential antigens, has not been well characterized. To study this, sera were obtained from five pediatric patients with acute diarrhe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5090-5095.2000
更新日期:2000-09-01 00:00:00
abstract::We describe here a Sprague-Dawley rat model for chronic osteomyelitis. Staphylococcus aureus and sodium morrhuate were implanted by either microdrilling or direct needle injection into the tibiae of rats. Of 107 rats, 87 (81%) developed osteomyelitis when a high-speed drill was used for implantation, and 27 (51%) of 5...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.581-586.1985
更新日期:1985-03-01 00:00:00
abstract::Yersinia pestis KIM5 was found to be cytotoxic for the IC21 and P388D1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from C57BL/6 mice. Affected cells phagocytosed KIM5 inefficiently, became spherical, detached readily from culture dishes, and retained 51Cr poorly. The cytotoxic effect wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.788-794.1986
更新日期:1986-03-01 00:00:00
abstract::Pseudomonas aeruginosa exotoxin A (PA), a potent protein synthesis inhibitor, was found to be a weak T-cell mitogen for murine splenocytes. Maximal stimulation of [3H]thymidine incorporation was obtained with 10 to 100 ng of toxin per ml following a 4-day induction. PA was also shown to be a polyclonal activator of cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.213-218.1988
更新日期:1988-01-01 00:00:00
abstract::Aspergillus fumigatus produces conidia that are highly dispersable and resistant to degradation. We have sought to analyze these properties by studying the rodlets which form the outer spore coat protein. Degenerate primers based on hydrophobins in other fungi were applied to genomic DNA from A. fumigatus in PCR. A pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4389-4395.1994
更新日期:1994-10-01 00:00:00