Deletion of Interleukin-4 Receptor Alpha-Responsive Keratinocytes in BALB/c Mice Does Not Alter Susceptibility to Cutaneous Leishmaniasis.


:The skin microenvironment at the site of infection plays a role in the early events that determine protective T helper 1/type 1 immune responses during cutaneous leishmaniasis (CL) infection. During CL in nonhealing BALB/c mice, early interleukin-4 (IL-4) can instruct dendritic cells for protective Th1 immunity. Additionally, keratinocytes, which are the principal cell type in the skin epidermis, have been shown to secrete IL-4 early after Leishmania major infection. Here, we investigated whether IL-4/IL-13 signaling via the common IL-4 receptor alpha chain (IL-4Rα) on keratinocytes contributes to susceptibility during experimental CL. To address this, keratinocyte-specific IL-4Rα-deficient (KRT14cre IL-4Rα-/lox) mice on a BALB/c genetic background were generated by gene targeting and site-specific recombination (Cre/loxP) under the control of the keratinocyte-specific krt14 locus. Following high-dose infection with L. major IL-81 and LV39 promastigotes subcutaneously in the footpad, footpad swelling, parasite burden, IFN-γ/IL-4/IL-13 cytokine production, and type 1 and type 2 antibody responses were similar between KRT14cre IL-4Rα-/lox and littermate control IL-4Rα-/lox BALB/c mice. An intradermal infection with low-dose L. major IL-81 and LV39 promastigotes in the ear showed results in infected KRT14cre IL-4Rα-/lox BALB/c mice similar to those of littermate control IL-4Rα-/lox BALB/c mice, with the exception of a significant decrease observed in parasite burden only at the site of LV39 infection in the ear. Collectively, our results show that autocrine and paracrine signaling of IL-4/IL-13 through the IL-4Rα chain on keratinocytes does not influence the establishment of a nonhealing Th2 immune response in BALB/c mice during L. major infection.


Infect Immun


Infection and immunity


Govender M,Hurdayal R,Martinez-Salazar B,Gqada K,Pillay S,Gcanga L,Passelli K,Nieuwenhuizen NE,Tacchini-Cottier F,Guler R,Brombacher F




Has Abstract


2018-11-20 00:00:00












  • Enzymatic degradation of H2O2 by Leptospira.

    abstract::The enzymes responsible for reducing H2O2 were surveyed in 49 strains of Leptospira by using semiquantitative assays for catalase and peroxidase. The survey revealed a differential distribution of catalase and peroxidase activities between the two leptospiral complexes. The pathogenic Leptospira interrogans strains ga...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Corin RE,Boggs E,Cox CD

    更新日期:1978-12-01 00:00:00

  • Characterization of an anticryptococcal protein isolated from human serum.

    abstract::Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sridhar S,Ahluwalia M,Brummer E,Stevens DA

    更新日期:2000-06-01 00:00:00

  • Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin.

    abstract::The in vitro effects of pneumolysin, a sulfhydryl-activated toxin produced by Streptococcus pneumoniae, on various functions of human polymorphonuclear leukocytes (PMNLs) was investigated. Treatment of PMNLs with highly purified toxin significantly inhibited respiratory burst (in response to stimulation), ability to k...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Paton JC,Ferrante A

    更新日期:1983-09-01 00:00:00

  • GNA33 of Neisseria meningitidis is a lipoprotein required for cell separation, membrane architecture, and virulence.

    abstract::GNA33 is a membrane-bound lipoprotein with murein hydrolase activity that is present in all Neisseria species and well conserved in different meningococcal isolates. The protein shows 33% identity to a lytic transglycolase (MltA) from Escherichia coli and has been shown to be involved in the degradation of both insolu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Adu-Bobie J,Lupetti P,Brunelli B,Granoff D,Norais N,Ferrari G,Grandi G,Rappuoli R,Pizza M

    更新日期:2004-04-01 00:00:00

  • Designing therapies against experimental visceral leishmaniasis by modulating the membrane fluidity of antigen-presenting cells.

    abstract::The membrane fluidity of antigen-presenting cells (APCs) has a significant bearing on T-cell-stimulating ability and is dependent on the cholesterol content of the membrane. The relationship, if any, between membrane fluidity and defective cell-mediated immunity in visceral leishmaniasis has been investigated. Systemi...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Banerjee S,Ghosh J,Sen S,Guha R,Dhar R,Ghosh M,Datta S,Raychaudhury B,Naskar K,Haldar AK,Lal CS,Pandey K,Das VN,Das P,Roy S

    更新日期:2009-06-01 00:00:00

  • Division of single host cells after infection with chlamydiae.

    abstract::Mouse fibroblasts (L cells) were infected in suspension with Chlamydia psittaci (6BC) and then plated out on a solid substrate at a density of 80 cells per cm2 so that the effect of chlamydial infection on the division of single host cells and their progeny could be determined. Uninfected L cells multiplied with a mea...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Horoschak KD,Moulder JW

    更新日期:1978-01-01 00:00:00

  • The kinetoplastid membrane protein 11 of Leishmania donovani and African trypanosomes is a potent stimulator of T-lymphocyte proliferation.

    abstract::Kinetoplastid membrane protein 11 (KMP-11) from Leishmania donovani is an abundant 11-kDa surface membrane glycoprotein. Lymph node cells from mice of six different H-2 haplotypes immunized with KMP-11 or with L. donovani promastigotes were stimulated to proliferate in vitro KMP-11. Primed purified T cells required an...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tolson DL,Jardim A,Schnur LF,Stebeck C,Tuckey C,Beecroft RP,Teh HS,Olafson RW,Pearson TW

    更新日期:1994-11-01 00:00:00

  • Genetic analysis of the 9.5-kilobase virulence plasmid of Yersinia pestis.

    abstract::The 9.5-kilobase plasmid of Yersinia pestis determines plasminogen activator, coagulase, pesticin, and pesticin immunity activities. We have mapped and cloned the loci encoding these activities and demonstrated that both plasminogen activator and coagulase were determined by the same gene, designated pla. The primary ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sodeinde OA,Goguen JD

    更新日期:1988-10-01 00:00:00

  • ActA is required for crossing of the fetoplacental barrier by Listeria monocytogenes.

    abstract::The facultative intracellular bacterial pathogen Listeria monocytogenes induces severe fetal infection during pregnancy. Little is known about the molecular mechanisms allowing the maternofetal transmission of bacteria. In this work, we studied fetoplacental invasion by infecting mice with various mutants lacking viru...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Le Monnier A,Autret N,Join-Lambert OF,Jaubert F,Charbit A,Berche P,Kayal S

    更新日期:2007-02-01 00:00:00

  • Role of viremia in the suppression of T-cell function during murine cytomegalovirus infection.

    abstract::The suppression of T-cell deoxyribonucleic acid (DNA) synthesis by serum from mice acutely with murine cytomegalovirus (MCMV) was investigated. Spleen cells from uninfected mice were exposed to concanavalin A in the presence of serum taken from mice at various times after infection with MCMV. The capacity of the serum...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Booss J,Wheelock EF

    更新日期:1977-08-01 00:00:00

  • Influenza virus population dynamics in the respiratory tract of experimentally infected mice.

    abstract::Virus population dynamics in the lungs, trachea, and nasopharynx of Swiss-ICR mice were studied after respiratory challenge with mouse-adapted preparations of strain A2/Aichi/2/68 influenza virus. Markedly higher doses of virus were required to produce infection with nasopharyngeal challenge than with bronchoalveolar ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Larson EW,Dominik JW,Rowberg AH,Higbee GA

    更新日期:1976-02-01 00:00:00

  • Chronic infection with Trypanosoma musculi in congenitally athymic nude mice.

    abstract::Trypanosoma musculi produces a chronic infection with a consistently elevated parasitemia in nude mice. Thymic reconstitution of nude mice restores immunity to the infection. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rank RG,Roberts DW,Weidanz WP

    更新日期:1977-05-01 00:00:00

  • Virulence factors determine attachment and ingestion of nonopsonized and opsonized Bordetella pertussis by human monocytes.

    abstract::In the present study, the role of virulence factors in and the effect of opsonization on the interactions between Bordetella pertussis and human monocytes were investigated. The methods used facilitated the distinction between attachment and ingestion of bacteria by monocytes. Nonopsonized virulent B. pertussis cells ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hazenbos WL,van den Berg BM,van't Wout JW,Mooi FR,van Furth R

    更新日期:1994-11-01 00:00:00

  • Borrelia burgdorferi RNA induces type I and III interferons via Toll-like receptor 7 and contributes to production of NF-κB-dependent cytokines.

    abstract::Borrelia burgdorferi elicits a potent cytokine response through activation of multiple signaling receptors on innate immune cells. Spirochetal lipoproteins initiate expression of NF-κB-dependent cytokines primarily via TLR2, whereas type I interferon (IFN) production is induced through the endosomal receptors TLR7 and...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Love AC,Schwartz I,Petzke MM

    更新日期:2014-06-01 00:00:00

  • Magnetic resonance imaging of pulmonary lesions in guinea pigs infected with Mycobacterium tuberculosis.

    abstract::We utilized magnetic resonance imaging to visualize lesions in the lungs of guinea pigs infected by low-dose aerosol exposure to Mycobacterium tuberculosis. Lesions were prominent in such images, and colorized three-dimensional reconstructions of images revealed a very uniform distribution in the lungs. Lesion numbers...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kraft SL,Dailey D,Kovach M,Stasiak KL,Bennett J,McFarland CT,McMurray DN,Izzo AA,Orme IM,Basaraba RJ

    更新日期:2004-10-01 00:00:00

  • The acylated form of protein D of Haemophilus influenzae is more immunogenic than the nonacylated form and elicits an adjuvant effect when it is used as a carrier conjugated to polyribosyl ribitol phosphate.

    abstract::The nonacylated form of protein D (PDm) of Haemophilus influenzae has been shown to induce the production of antibodies that are bactericidal to homologous and heterologous nontypeable H. influenzae (NTHi) strains. In this study, immunization of rats with lipoprotein D (LPD) induced higher levels of anti-protein D imm...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Akkoyunlu M,Melhus A,Capiau C,van Opstal O,Forsgren A

    更新日期:1997-12-01 00:00:00

  • The Pneumococcal Surface Proteins PspA and PspC Sequester Host C4-Binding Protein To Inactivate Complement C4b on the Bacterial Surface.

    abstract::Complement is a critical component of antimicrobial immunity. Various complement regulatory proteins prevent host cells from being attacked. Many pathogens have acquired the ability to sequester complement regulators from host plasma to evade complement attack. We describe here how Streptococcus pneumoniae adopts a st...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Haleem KS,Ali YM,Yesilkaya H,Kohler T,Hammerschmidt S,Andrew PW,Schwaeble WJ,Lynch NJ

    更新日期:2018-12-19 00:00:00

  • Treponema denticola outer membrane inhibits calcium flux in gingival fibroblasts.

    abstract::Treponema denticola is a cultivable oral spirochete which perturbs the cytoskeleton in cultured cells of oral origin, but intracellular signalling pathways by which it affects actin assembly are largely unknown. As the outer membrane (OM) of Treponema denticola disrupts actin-dependent processes that normally require ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ko KS,Glogauer M,McCulloch CA,Ellen RP

    更新日期:1998-02-01 00:00:00

  • Ontogeny of the phytohemagglutinin response of fetal piglet thymic and splenic lymphocytes.

    abstract::Lymphocytes derived from 72- to 90-day-old fetal piglet thymuses respond in vitro to phytohemagglutinin quantitatively greater than do spleen cell lymphocytes. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rodey GE,Day N,Holmes-Gray B,Good RA

    更新日期:1972-03-01 00:00:00

  • Polyclonal B cell activation in hamsters infected with parasites of the genus Leishmania.

    abstract::Mesocricetus auratus (golden hamsters) infected with leishmania developed characteristic B cell immune responses that depended on the infecting species of leishmania. Thus, hamsters infected with viscerotropic leishmania (Leishmania donovani) developed antileishmania antibodies and hypergammaglobulinemia due to polycl...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Campos-Neto A,Bunn-Moreno MM

    更新日期:1982-12-01 00:00:00

  • Fusobacterium nucleatum increases collagenase 3 production and migration of epithelial cells.

    abstract::Fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. Initiation and outcome of infective diseases depend critically on the host cell signaling system altered by th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Uitto VJ,Baillie D,Wu Q,Gendron R,Grenier D,Putnins EE,Kanervo A,Firth JD

    更新日期:2005-02-01 00:00:00

  • Vaccination of chickens with strain CVL30, a genetically defined Salmonella enteritidis aroA live oral vaccine candidate.

    abstract::Newly hatched chicks were vaccinated orally with a genetically defined Salmonella enteritidis aroA candidate, strain CVL30. In chickens immunized with 10(5) or 10(9) CFU and challenged by the intravenous route with 10(8) CFU of S. enteritidis 109 Nalr at 8 weeks old, there were similar reductions in colonization of th...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cooper GL,Venables LM,Woodward MJ,Hormaeche CE

    更新日期:1994-11-01 00:00:00

  • Differential regulation of in vitro humoral and cellular immune responsiveness in Brugia pahangi-infected jirds.

    abstract::Patent filarial infection is associated with the downregulation of parasite-specific immune reactivity. In the present study, the relationship between in vitro parasite antigen-specific humoral and cellular immune responsiveness was investigated in Brugia pahangi-infected jirds and in jirds immunized with soluble anti...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Prier RC,Lammie PJ

    更新日期:1988-12-01 00:00:00

  • Immunochemical analysis of streptococcal group A, B, and C carbohydrates, with emphasis on group A.

    abstract::Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis,...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fung JC,Wicher K,McCarty M

    更新日期:1982-07-01 00:00:00

  • Identification and characterization of an autolysin-encoding gene of Streptococcus mutans.

    abstract::We identified a gene (atlA) encoding autolytic activity from Streptococcus mutans Xc. The AtlA protein predicted to be encoded by atlA is composed of 979 amino acids with a molecular weight of 107,279 and has a conserved beta-1,4-N-acetylmuramidase (lysozyme) domain in the C-terminal portion. Sodium dodecyl sulfate ex...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shibata Y,Kawada M,Nakano Y,Toyoshima K,Yamashita Y

    更新日期:2005-06-01 00:00:00

  • Lack of immune responses to Mycobacterium tuberculosis DosR regulon proteins following Mycobacterium bovis BCG vaccination.

    abstract::Mycobacterium bovis BCG is widely used as a vaccine against tuberculosis (TB), despite its variable protective efficacy. Relatively little is known about the immune response profiles following BCG vaccination in relation to protection against TB. Here we tested whether BCG vaccination results in immune responses to Do...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lin MY,Geluk A,Smith SG,Stewart AL,Friggen AH,Franken KL,Verduyn MJ,van Meijgaarden KE,Voskuil MI,Dockrell HM,Huygen K,Ottenhoff TH,Klein MR

    更新日期:2007-07-01 00:00:00

  • A Subset of Protective γ9δ2 T Cells Is Activated by Novel Mycobacterial Glycolipid Components.

    abstract::γ9δ2 T cells provide a natural bridge between innate and adaptive immunity, rapidly and potently respond to pathogen infection in mucosal tissues, and are prominently induced by both tuberculosis (TB) infection and bacillus Calmette Guérin (BCG) vaccination. Mycobacterium-expanded γ9δ2 T cells represent only a subset ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Xia M,Hesser DC,De P,Sakala IG,Spencer CT,Kirkwood JS,Abate G,Chatterjee D,Dobos KM,Hoft DF

    更新日期:2016-08-19 00:00:00

  • Choriogonadotropin-like antigen in an anaerobic bacterium, Eubacterium lentum, isolated from a rectal tumor.

    abstract::Using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive mater...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Acevedo HF,Slifkin M,Pouchet-Melvin GR,Campbell-Acevedo EA

    更新日期:1979-06-01 00:00:00

  • Immunoglobulin A1 protease production by Haemophilus influenzae and Streptococcus pneumoniae.

    abstract::Bacterial strains of Haemophilus species and Streptococcus pneumoniae were examined for synthesis of the enzyme immunoglobulin A1 (IgA1) protease. Of 36 H. influenzae strains examined, 35 produced IgA1 protease; strains included all six capsular types, unencapsulated variants of types b and d, and untypable H. influen...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Male CJ

    更新日期:1979-10-01 00:00:00

  • Bacteroides fragilis: a possible cause of acute diarrheal disease in newborn lambs.

    abstract::Bacteroides fragilis with enterotoxin-like activity (BFEL) was isolated from the feces of 24- to 48-h-old lambs with acute diarrheal disease on three different sheep ranches in the Northern Rocky Mountain region of the United States. The lamb intestinal loop test was used to enrich for the bacterium before its initial...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Myers LL,Firehammer BD,Shoop DS,Border MM

    更新日期:1984-05-01 00:00:00