Abstract:
:The majority of strains of Actinobacillus actinomycetemcomitans isolated from patients with periodontal diseases secrete a leukotoxin that destroys human myeloid cells within minutes but has no effect on viability of peripheral blood lymphocytes in culture for 1.5 h. However, since this organism persists in the gingival crevice and thus may continuously release toxin over extended periods of time, we assessed the viability of T cells cultured with leukotoxin (0 to 250 ng/ml) for up to 2 days. Although the total numbers of cells recovered from cultures with or without leukotoxin were equivalent, leukotoxin killed up to 70% of the T cells in a time- and concentration-dependent manner. Cell death was associated with uptake of propidium iodide, release of 51Cr from the cytoplasm, and morphological evidence of damage to the plasma membrane and apoptosis. Leukotoxin also induced increased cleavage of chromosomal DNA into nucleosome-sized fragments, suggesting activation of an endogenous nuclease in the T cells. These data suggest that leukotoxin kills T cells by pathways resembling necrosis and programmed cell death. Leukotoxin-induced lymphotoxicity may represent a critical mechanism by which A. actinomycetemcomitans suppresses the host local immune response and contributes to the pathogenesis of diseases involving this microorganisms.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Mangan DF,Taichman NS,Lally ET,Wahl SMdoi
10.1128/IAI.59.9.3267-3272.1991subject
Has Abstractpub_date
1991-09-01 00:00:00pages
3267-72issue
9eissn
0019-9567issn
1098-5522journal_volume
59pub_type
杂志文章abstract::Antibody responses against proteins located on the surface or in the apical organelles of merozoites are presumed to be important components of naturally acquired protective immune responses against the malaria parasite Plasmodium falciparum. However, many merozoite antigens are highly polymorphic, and antibodies indu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.422-430.2005
更新日期:2005-01-01 00:00:00
abstract::Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of Trichomonas vaginalis. This two-dimensional substrate-SDS-PAGE resolved a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.157-162.1990
更新日期:1990-01-01 00:00:00
abstract::The attachment of radioisotope-labeled Mycoplasma pneumoniae to hamster tracheal rings in organ culture was examined by radioautography and liquid scintillation counting. Radioautographs of individual rings exposed for 8 h to (3H) thymidine-labeled virulent M. pneumoniae revealed a dense extracellular collection of em...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.959-966.1976
更新日期:1976-03-01 00:00:00
abstract::Assembly of adherent biofilms is the key mechanism involved in Staphylococcus epidermidis virulence during device-associated infections. Aside from polysaccharide intercellular adhesin (PIA), the accumulation-associated protein Aap and the extracellular matrix binding protein Embp act as intercellular adhesins, mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01142-10
更新日期:2011-06-01 00:00:00
abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.1106-1108.1976
更新日期:1976-10-01 00:00:00
abstract::A nonhuman primate model for malaria vaccine development allowing reliable, stringent sporozoite challenge and evaluation of both cellular and antibody responses is needed. We therefore constructed a multicomponent, multistage DNA vaccine for the simian malaria species Plasmodium knowlesi including two preerythrocytic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5565-5572.2001
更新日期:2001-09-01 00:00:00
abstract::Haemophilus ducreyi expresses 2 OmpA homologs, designated MOMP and OmpA2, whose genes are arranged in tandem on the chromosome. Northern blot analysis indicated that momp and ompA2 are transcribed independently. Sequences of the momp open reading frame (ORF) lacking the transcriptional start site were amplified by PCR...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2602-2607.2000
更新日期:2000-05-01 00:00:00
abstract::A synthetic medium capable of supporting growth of Vibrio parahaemolyticus is described. Growth yields and generation times were comparable to growth in a complex medium, although Kanagawa hemolysin was undetectable in the synthetic medium. Upon the addition of single amino acids to this synthetic medium, only D-trypt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
更新日期:1982-07-01 00:00:00
abstract::In meningococcal septic shock, the dominant inducer of inflammation is lipopolysaccharide (LPS) in the outer membrane of Neisseria meningitidis, while interleukin-10 (IL-10) is the principal anti-inflammatory cytokine. We have used microarrays and Ingenuity Pathway Analysis to study the global effects of IL-10 on gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00386-12
更新日期:2012-11-01 00:00:00
abstract::Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5384-5387.1993
更新日期:1993-12-01 00:00:00
abstract::Vero cytotoxin-producing Escherichia coli (VTEC) strains have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. Since adherence of enteric pathogens to epithelial surfaces is often a prerequisite for the subsequent delivery of bacterial enterotox...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
abstract::Endocarditis isolates of Enterococcus faecalis produced biofilm significantly more often than nonendocarditis isolates, and 39% of 79 versus 6% of 84 isolates produced strong biofilm (P < 0.0001). esp was not required, but its presence was associated with higher amounts of biofilm (P < 0.001). Mutants disrupted in dlt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3658-3663.2004
更新日期:2004-06-01 00:00:00
abstract::The hemolytic and sphingomyelinase C activities of supernatants of cultures of Leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. Both activities focused primarily at pH 8.1. Considered in conjunction with other circumstantial evidence, the results led to the concl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.262-264.1986
更新日期:1986-10-01 00:00:00
abstract::A simple and convenient method was developed for the preparation of Streptococcus pneumoniae type 14 polysaccharide (Pn14PS)-tetanus toxoid (TT) conjugate vaccines, using terminally linked Pn14PS fragments of different lengths. Native Pn14PS was simultaneously depolymerized and activated for conjugation by partial N-d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2441-2446.1998
更新日期:1998-06-01 00:00:00
abstract::Escherichia coli F-18, isolated from the feces of a healthy human in 1977, is an excellent colonizer of the streptomycin-treated mouse large intestine and displays normal motility and chemotaxis ability. A chemotaxis-defective derivative of E. coli F-18, E, coli F-18 CheA-, and a nonflagellated derivative, E. coli F-1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2957-2961.1990
更新日期:1990-09-01 00:00:00
abstract::Staphylococcus aureus is one of the pathogens most frequently isolated in device-related infections. S. aureus is equipped with surface-associated proteins promoting specific binding to matrix molecules. Clumping factor A (ClfA, encoded by clfA) mediates adhesion to fibrinogen. Whereas the contribution of ClfA to path...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2758-2762.2002
更新日期:2002-06-01 00:00:00
abstract::The lipid A portion has been identified as the active center responsible for lipopolysaccharide (LPS)-induced macrophage activation. However, we found that Salmonella (Salmonella enterica serovars Abortusequi, Minnesota, and Typhimurium) lipid A is inactive in human macrophages, despite its LPS being highly active. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6043-6047.2002
更新日期:2002-11-01 00:00:00
abstract::Active immunization of mice with high doses of heat-killed and autoclaved vaccine of unencapsulated strains of Staphylococcus aureus, which was grown in brain heart infusion media, protected against challenge with the Smith diffuse strain of Staphylococcus aureus. These organisms were capable of absorbing the protecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.939-942.1975
更新日期:1975-11-01 00:00:00
abstract::Our previous studies demonstrated that Mycobacterium bovis bacillus Calmette-Guérin (BCG) can directly interact with human NK cells and induce the proliferation, gamma interferon production, and cytotoxic activity of such cells without the need for accessory cells. Thus, the aim of the present study was to identify th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00870-07
更新日期:2008-04-01 00:00:00
abstract::Toxoplasma gondii infection in pregnant women may result in abortion or in fetal teratogenesis; however, the underlying mechanisms are still unclear. In this paper, based on a murine model, we showed that maternal infection with RH strain T. gondii tachyzoites induced elevated production of reactive oxygen species (RO...
journal_title:Infection and immunity
pub_type: 杂志文章,收录出版
doi:10.1128/IAI.06295-11
更新日期:2012-06-01 00:00:00
abstract::Trichomonas vaginalis infected with a double-stranded RNA virus undergoes phenotypic variation on the basis of surface versus cytoplasmic expression of the immunogenic protein P270. Examination of batch cultures by flow cytofluorometry with monoclonal antibody (MAb) to P270 yields both fluorescent and nonfluorescent t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.4298-4302.1999
更新日期:1999-08-01 00:00:00
abstract::Current efforts to develop plague vaccines focus on LcrV, a polypeptide that resides at the tip of type III secretion needles. LcrV-specific antibodies block Yersinia pestis type III injection of Yop effectors into host immune cells, thereby enabling phagocytes to kill the invading pathogen. Earlier work reported that...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05637-11
更新日期:2012-04-01 00:00:00
abstract::BCG, the attenuated strain of Mycobacterium bovis, has been widely used as a vaccine against tuberculosis and is thus an important candidate as a live carrier for multiple antigens. With the aim of developing a recombinant BCG (rBCG) vaccine against diphtheria, pertussis, and tetanus (DPT), we analyzed the potential o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.2.869-874.2001
更新日期:2001-02-01 00:00:00
abstract::Macrophage-dependent killing of facultative intracellular bacteria was markedly impaired by overt erythrocytic Plasmodium yoelii or Plasmodium berghei infection of mice. P. yoelii infection was capable of ablating not only the macrophage microbicidal capacity of "normal" animals but also the bactericidal capacities of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.396-407.1981
更新日期:1981-01-01 00:00:00
abstract::A T antigen preparation free of trypsin was obtained by application of CNBr-Sepharose linked to pure trypsin. Purification on an ion exchange chromatography column results in an electrophoretically homogeneous preparation of T protein. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.993-994.1976
更新日期:1976-03-01 00:00:00
abstract::Studies on the innate immune response against microbial infections in Drosophila melanogaster involve mutant strains and their reference strains that act as experimental controls. We used five standard D. melanogaster laboratory reference strains (Oregon R, w1118, Canton-S, Cinnabar Brown, and Yellow White [YW]) and i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02318-14
更新日期:2014-10-01 00:00:00
abstract::Tritium-labeled, water-soluble components of Streptococcus pyogenes type M6 absorbed to cardiac tissue in vitro. Tissue binding was time dependent, saturable, and reversible. Chromatography of the crude bacterial extract on Bio-Gel P-300 indicated a molecular weight greater than 300,000 for the heart-binding component...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.335-342.1982
更新日期:1982-01-01 00:00:00
abstract::Sera from certain animal species contain a substance(s) which binds hepatitis B surface antigen. The hepatitis B binding substance found in animals is not antibody, but appears to be a glycoprotein which reacted with antigen-coated beads and produced a "false positive" test for antibody. This glycoprotein could be sel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.879-885.1978
更新日期:1978-09-01 00:00:00
abstract::Cryptosporidial infections in severe combined immune deficient (SCID) mice produce a chronic disease state which in the later stages leads to extraintestinal involvement and hepatic dysfunction. To further characterize the infection dynamics in this model and monitor the changes in the hepatic system, a dose titration...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1691-1695.1994
更新日期:1994-05-01 00:00:00
abstract::We previously showed the requirement of both T cells and gamma interferon (IFN-gamma)-producing non-T cells for the genetic resistance of BALB/c mice to the development of toxoplasmic encephalitis (TE). In order to define the role of IFN-gamma production and the perforin-mediated cytotoxicity of T cells in this resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4432-4438.2004
更新日期:2004-08-01 00:00:00