Abstract:
:We previously demonstrated that plasmid-deficient Chlamydia muridarum retains the ability to infect the murine genital tract but does not elicit oviduct pathology because it fails to activate Toll-like receptor 2 (TLR2). We derived a plasmid-cured derivative of the human genital isolate Chlamydia trachomatis D/UW-3/Cx, strain CTD153, which also fails to activate TLR2, indicating this virulence phenotype is associated with plasmid loss in both C. trachomatis and C. muridarum. As observed with plasmid-deficient C. muridarum, CTD153 displayed impaired accumulation of glycogen within inclusions. Transcriptional profiling of the plasmid-deficient strains by using custom microarrays identified a conserved group of chromosomal loci, the expression of which was similarly controlled in plasmid-deficient C. muridarum strains CM972 and CM3.1 and plasmid-deficient C. trachomatis CTD153. However, although expression of glycogen synthase, encoded by glgA, was greatly reduced in CTD153, it was unaltered in plasmid-deficient C. muridarum strains. Thus, additional plasmid-associated factors are required for glycogen accumulation by this chlamydial species. Furthermore, in C. trachomatis, glgA and other plasmid-responsive chromosomal loci (PRCLs) were transcriptionally responsive to glucose limitation, indicating that additional regulatory elements may be involved in the coordinated expression of these candidate virulence effectors. Glucose-limited C. trachomatis displayed reduced TLR2 stimulation in an in vitro assay. During human chlamydial infection, glucose limitation may decrease chlamydial virulence through its effects on plasmid-responsive chromosomal genes.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
O'Connell CM,AbdelRahman YM,Green E,Darville HK,Saira K,Smith B,Darville T,Scurlock AM,Meyer CR,Belland RJdoi
10.1128/IAI.01118-10subject
Has Abstractpub_date
2011-03-01 00:00:00pages
1044-56issue
3eissn
0019-9567issn
1098-5522pii
IAI.01118-10journal_volume
79pub_type
杂志文章abstract::One-day-old chicks were infected orally with two strains of weakly hemolytic spirochetes isolated from a human and a pig with intestinal spirochetosis. These spirochetosis both colonized birds, attached end-on to their cecal enterocytes, induced watery diarrhea, and significantly depressed growth rates. Cultures of Se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3705-3710.1995
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abstract::The human pathogen enterohemorrhagic Escherichia coli (EHEC) O157:H7 has two histidine sensor kinases, QseC and QseE, which respond to the mammalian adrenergic hormones epinephrine and norepinephrine by increasing their autophosphorylation. Although QseC and QseE are present in nonpathogenic strains of E. coli, EHEC e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05921-11
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5908-5914.2005
更新日期:2005-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.86-91.1975
更新日期:1975-01-01 00:00:00
abstract::Schistosoma mansoni eggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.01335-13
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.4185-4187.1995
更新日期:1995-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2327-2331.1987
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.767-773.1997
更新日期:1997-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.1065-1073.1991
更新日期:1991-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.84-90.1977
更新日期:1977-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1384-1390.1990
更新日期:1990-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1774-1778.1987
更新日期:1987-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1943-1946.2001
更新日期:2001-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5107-5112.1998
更新日期:1998-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5015-5021.1996
更新日期:1996-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.102-106.1981
更新日期:1981-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2609-2614.1991
更新日期:1991-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1182-1186.1975
更新日期:1975-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6582-6590.2003
更新日期:2003-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00570-17
更新日期:2017-10-18 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2284-2288.1993
更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5483-5486.2004
更新日期:2004-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.378-381.1978
更新日期:1978-11-01 00:00:00
abstract::Brucella abortus endotoxin preparations, containing approximately 5 to 6% protein, induce strong immune and adjuvant immunoglobulin G (IgG) responses as compared with Escherichia coli endotoxin preparations, with equivalent amounts of protein, which induce responses in which IgM antibody predominates. Using an enzyme-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.728-734.1986
更新日期:1986-12-01 00:00:00
abstract::The absolute requirement for elemental iron and the porphyrin nucleus for growth of Haemophilus influenzae led us to investigate the role of iron and hemin in regulation of expression of the H. influenzae transferrin receptor. H. influenzae type b strain H1689 was grown in brain heart infusion broth supplemented with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4033-4037.1993
更新日期:1993-10-01 00:00:00
abstract::Serine proteases and serine protease homologs form the second largest gene family in the Drosophila melanogaster genome. Certain genes in the Jonah multigene family encoding serine proteases have been implicated in the fly antiviral immune response. Here, we report the involvement of Jonah66Ci in the Drosophila immune...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-19
更新日期:2019-08-21 00:00:00
abstract::Sublethal infection of mice with recombinant Listeria monocytogenes expressing a model epitope in either secreted or nonsecreted form results in similar CD8(+) T-cell priming. Since nonsecreted bacterial proteins have no obvious access to the endogenous major histocompatibility complex (MHC) class I presentation pathw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2196-2204.2000
更新日期:2000-04-01 00:00:00
abstract::Phospholipase A2 and lysophospholipase activities were detected in the culture supernatant fluids of a virulent strain of Vibrio vulnificus. The phospholipase A2 was inactivated by heating at 56 degrees C for 30 min, had an apparent molecular weight of greater than or equal to 80,000 (estimated by gel filtration with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.458-463.1984
更新日期:1984-08-01 00:00:00