Different sugar compositions of lipopolysaccharides isolated from phase I and pure phase II cells of Coxiella burnetii.

Abstract:

:The compositions of lipopolysaccharides I and II, isolated from Coxiella burnetii phase I and pure phase II cells, respectively, showed drastic and clear-cut differences. Lipopolysaccharide II exhibited a comparatively simple composition. It contained, in addition to glucosamine (from lipid A) and a 2-keto-3-deoxyoctulosonic acid-related, thiobarbituric acid-positive substance, only two aldoses in high amounts, namely D-mannose and D-glycero-D-mannoheptose. Lipopolysaccharide I contained the same components, but it also contained at least eight other constituents including neutral sugars, e.g., an unusual branched sugar (3-C-methyl-6-deoxy-hexose) and unusual aminohexoses. Some components were characterized by a very high retention time in gas-liquid chromatography. The similarity of the phase variation in C. burnetii to the well-known smooth-to-rough mutation found in Enterobacteriaceae is discussed. Cells of phase I correspond to enterobacterial smooth forms, whereas cells of phase II might be compared to the rough forms.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Schramek S,Mayer H

doi

10.1128/IAI.38.1.53-57.1982

subject

Has Abstract

pub_date

1982-10-01 00:00:00

pages

53-7

issue

1

eissn

0019-9567

issn

1098-5522

journal_volume

38

pub_type

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