Abstract:
:A synthetic peptide whose sequence was derived from the circumsporozoite protein of Plasmodium knowlesi coupled to bovine gamma globulin has been shown to be immunogenic when administered with Freund complete adjuvant. The present experiments were designed to test the immunogenicity of the peptide when attached to a tetanus toxoid carrier and administered with alum or murabutide, both acceptable clinical adjuvants. In both cases, the use of an adjuvant increased the levels of circulating anti-peptide antibodies over those observed when no adjuvant was used. However, when the antisera were tested for reactivity with the native protein, animals of the group receiving the conjugate associated with murabutide always had titers greatly exceeding those observed in animals that received the conjugate with alum. Moreover, the sera of the murabutide-treated group were shown to be more active in eliciting shedding of the circumsporozoite protein than were sera of animals of the Freund complete adjuvant-treated group. The use of tetanus toxoid in secondary immunizations could be eliminated when the mice primed with peptide-tetanus toxoid and murabutide were boosted with a polymer of the peptide. The results indicate that the synthetic malarial peptide-tetanus toxoid conjugate is capable of stimulating high levels of biologically active antibodies only when administered with murabutide.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Clough ER,Audibert FM,Barnwell JW,Schlesinger DH,Arnon R,Chedid LAdoi
10.1128/IAI.48.3.839-842.1985subject
Has Abstractpub_date
1985-06-01 00:00:00pages
839-42issue
3eissn
0019-9567issn
1098-5522journal_volume
48pub_type
杂志文章abstract::We have cloned chromosomal genes mediating the aerobactin iron transport system from the enteroinvasive strain Escherichia coli 978-77. The physical map of the region spanning the siderophore biosynthesis genes and the upstream portion of the receptor gene in strain 978-77-derived clones was identical to the correspon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.357-364.1991
更新日期:1991-01-01 00:00:00
abstract::The survival of Staphylococcus aureus in the lungs of mice was studied under various conditions. Doses of 10(7) to 10(9) washed staphylococci were quantitatively introduced into the lungs after intratracheal inoculation in mice under either ether or sodium pentobarbital anesthesia. Mice were sacrificed at intervals, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.1.114-123.1978
更新日期:1978-07-01 00:00:00
abstract::Immunoprecipitation of radiolabeled antigens with bovine antisera indicated that many Babesia bigemina and Babesia bovis merozoite proteins contain isolate-common epitopes, while at least 16 B. bigemina and 8 B. bovis proteins contain species-cross-reactive epitopes. One immunogenic, isolate-common, and species-specif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1658-1660.1988
更新日期:1988-06-01 00:00:00
abstract::Bacteria exhibit extensive genetic heterogeneity within species. In many cases, these differences account for virulence properties unique to specific strains. Several such loci have been discovered in the genome of the type b serotype of Haemophilus influenzae, a human pathogen able to cause meningitis, pneumonia, and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1098-1108.2003
更新日期:2003-03-01 00:00:00
abstract::Nasopharyngeal colonization represents the initial interaction between Haemophilus influenzae and its human host. Factors that influence bacterial carriage likely affect transmission and incidence of infection. Therefore, we investigated host factors involved in limiting H. influenzae colonization in BALB/c mice, as c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01564-08
更新日期:2009-08-01 00:00:00
abstract::Mice infected with Trypanosoma musculi developed hyperplasia of the spleen, lymph nodes, and liver; in contrast, their thymuses displayed transient involution. All organs returned to normal in a month or less. There was modest anemia, lasting until the parasites were cleared from the bloodstream, followed by a rapid i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.1008-1017.1981
更新日期:1981-12-01 00:00:00
abstract::An experimental model of coxsackievirus B3 infection in newborn mice was utilized to examine the protective role of antiviral antibodies and an interferon inducer, polyinosinic acid-polycytidylic acid [poly(I:C)]. Subcutaneous administration to the infected mice of specific antiviral antibodies resulted in significant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.720-727.1982
更新日期:1982-08-01 00:00:00
abstract::Mutant cultures that lacked the ability to produce filamentous hemagglutinin were obtained by UV irradiation of Bordetella pertussis strain 18-323. Various biological activities of these mutants were compared with those of the parent culture. It was found that the leukocytosis-promoting, histamine-sensitizing, and mou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1018-1023.1982
更新日期:1982-03-01 00:00:00
abstract::Streptococcus mutans produces several enzymes which metabolize sucrose. Three glucosyltransferase genes (gtfB, gtfC, and gtfD) and a single fructosyltransferase gene (ftf) encode enzymes which are important in formation of exopolysaccharides. Mutants of S. mutans V403 carrying single and multiple mutations of the gtfB...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2316-2323.1991
更新日期:1991-07-01 00:00:00
abstract::Rabbit lymphoid cells from spleen, peripheral blood, and peritoneal cavity lacked killer (K)-cell activity against cell lines of rabbit and human origin, including virus-infected human tumor cells. This lack of activity was not affected by antibody concentration, source of antibodies, effector/target cell ratio, or le...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.489-496.1982
更新日期:1982-02-01 00:00:00
abstract::The role of a plasmid in the virulence activity of an enteropathogenic Escherichia coli (EPEC) strain belonging to serotype 0111:NM was examined. EPEC strain B171, which is resistant to chloramphenicol, streptomycin, sulfathiazole, and tetracycline, harbors a 54-megadalton plasmid, pYR111, and exhibits localized adher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2052-2056.1987
更新日期:1987-09-01 00:00:00
abstract::In the present study, the immunodepressive effects of canine distemper virus (CDV) infection of dogs on two parameters of lymphocyte function, namely phytomitogen-induced cellular proliferation and skin allograft rejection, were investigated. Infection of susceptible gnotobiotic dogs with virulent R252-CDV resulted in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1069-1078.1975
更新日期:1975-05-01 00:00:00
abstract::Lipopolysaccharide (LPS) binding protein (LBP) is an acute-phase protein that enhances the responsiveness of immune cells to LPS by virtue of its capacity to transfer LPS to CD14. To determine the role of LBP in the innate immune response to peritonitis, LBP gene-deficient (LBP(-/-)) and normal wild-type mice were int...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.6747-6753.2003
更新日期:2003-12-01 00:00:00
abstract::Fibrosis is a significant complication of intestinal disorders associated with microbial dysbiosis and pathobiont expansion, notably Crohn's disease (CD). Mechanisms that favor fibrosis are not well understood, and therapeutic strategies are limited. Here we demonstrate that colitis-susceptible Il10-deficient mice dev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00587-19
更新日期:2019-10-18 00:00:00
abstract::Murine bone marrow-derived macrophages were infected with a panel of virulent isolates of Mycobacterium tuberculosis including laboratory strains Erdman and H37Rv and various clinical isolates in order to determine the sensitivity of each of these strains to the antimycobacterial activities of macrophage-generated rea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1189-1195.1997
更新日期:1997-04-01 00:00:00
abstract::Passive transfer of antibody may be useful for preexposure prophylaxis against biological agents used as weapons of terror, such as Bacillus anthracis. Studies were performed to evaluate the ability of anthrax antiprotective antigen (anti-PA) and antilethal factor (anti-LF) neutralizing monoclonal antibodies (mAbs) to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00529-07
更新日期:2007-11-01 00:00:00
abstract::Actinlike material was obtained from disrupted Pseudomonas aeruginosa cells by a modification of the method of Hancock and Nikaido (J. Bacteriol. 136:381-390) for isolating outer membrane vesicles. Pelleted membranes were dissolved in Laemmli sample buffer and electrophoresed in parallel lanes with purified rabbit ske...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.483-487.1985
更新日期:1985-11-01 00:00:00
abstract::Oophorectomized, estrogen-treated rats were immunized by the intravaginal or intranasal route with a mannoprotein extract (MP) or secreted aspartyl proteinases (Sap) of Candida albicans, with or without cholera toxin as a mucosal adjuvant. Both routes of immunization were equally effective in (i) inducing anti-MP and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2725-2729.2002
更新日期:2002-05-01 00:00:00
abstract::Polymorphonuclear leukocytes (PMN) from patients with chronic granulomatous disease (CGD) fail to produce microbicidal concentrations of reactive oxygen species (ROS) due to mutations in NOX2. Patients with CGD suffer from severe, life-threatening infections and inflammatory complications. Granulibacter bethesdensis i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00778-15
更新日期:2015-11-01 00:00:00
abstract::Infection by Shigella flexneri is characterized by infiltration of neutrophils in the intestinal mucosa and by a strong inflammatory reaction. Although neutrophils are constitutively programmed to die by apoptosis, we show that isolated human neutrophils undergo necrosis 2 h after infection with virulent S. flexneri s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1289-1296.2000
更新日期:2000-03-01 00:00:00
abstract::Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were interme...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00885-08
更新日期:2008-12-01 00:00:00
abstract::Random minitransposon mutagenesis was used to identify genes involved in the survival of Bordetella bronchiseptica within eukaryotic cells. One of the mutants which exhibited a reduced ability to survive intracellularly harbored a minitransposon insertion in a locus (ris) which displays a high degree of homology to tw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.4640-4650.1998
更新日期:1998-10-01 00:00:00
abstract::Subtilase cytotoxin (SubAB), which is produced by certain strains of Shiga-toxigenic Escherichia coli (STEC), causes the 78-kDa glucose-regulated protein (GRP78/BiP) cleavage, followed by induction of endoplasmic reticulum (ER) stress, leading to caspase-dependent apoptosis via mitochondrial membrane damage by Bax/Bak...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01020-10
更新日期:2011-02-01 00:00:00
abstract::Nitration of protein A with tetranitromethane incompletely blocks its Fc-binding ability for immunoglobulin G when measured by more sensitive methods of immunoprecipitation and when tested in the enzyme-linked immunosorbent assay. In addition, binding of protein A and blockage by nitration is heterogeneous and depends...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.659-662.1978
更新日期:1978-08-01 00:00:00
abstract::Neisseria gonorrhoeae is the causative agent of the sexually transmitted disease gonorrhea, and infection with this organism is typically associated with an intense inflammatory response. In many individuals, however, the infection is asymptomatic and can progress to serious secondary complications. The type IV pili o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.442-448.2006
更新日期:2006-01-01 00:00:00
abstract::Potential vaccine components for protection against atrophic rhinitis in pigs were developed. This was achieved by deletion mutagenesis of the gene encoding the Pasteurella multocida toxin. Four purified toxin derivatives lacking different and widely separated regions in the amino acid sequence were characterized by a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1387-1393.1991
更新日期:1991-04-01 00:00:00
abstract::A recombinant 23-kDa protein (rBPI23) derived from human bactericidal/permeability-increasing protein (BPI) possesses potent endotoxin-neutralizing abilities in vitro and in vivo. Binding of rBPI23 to those endotoxins (lipopolysaccharides [LPSs]) encountered clinically would be a prerequisite for efficacy in decreasin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3564-3567.1994
更新日期:1994-08-01 00:00:00
abstract::Three monoclonal antibodies (MAbs), all of the immunoglobulin G1 subclass, were raised against Cryptococcus neoformans by using the technique of cyclophosphamide ablation of B-cell responses against shared epitopes of the cross-reactive fungus Trichosporon beigelii. MAb 3C2 was reactive against the encapsulated and no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.143-149.1992
更新日期:1992-01-01 00:00:00
abstract::The in vitro effects of Pseudomonas aeruginosa elastase (P. aeruginosa E) and of human leukocyte elastase on human airway lysozyme (HAL) were investigated. P. aeruginosa E inactivated and cleaved HAL, whereas human leukocyte elastase had no effect. Total inactivation of HAL by P. aeruginosa E was observed after 120 mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.2.555-560.1985
更新日期:1985-02-01 00:00:00
abstract::Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3787-3791.2000
更新日期:2000-06-01 00:00:00