Abstract:
:Lipopolysaccharide (LPS) binding protein (LBP) is an acute-phase protein that enhances the responsiveness of immune cells to LPS by virtue of its capacity to transfer LPS to CD14. To determine the role of LBP in the innate immune response to peritonitis, LBP gene-deficient (LBP(-/-)) and normal wild-type mice were intraperitoneally infected with Escherichia coli, the most common causative pathogen of this disease. LBP was detected at low concentrations in peritoneal fluid of healthy wild-type mice, and the local LBP levels increased rapidly upon induction of peritonitis. LBP(-/-) mice were highly susceptible to E. coli peritonitis, as indicated by accelerated mortality, earlier bacterial dissemination to the blood, impaired bacterial clearance in the peritoneal cavity, and more severe remote organ damage. LBP(-/-) mice displayed diminished early tumor necrosis factor alpha, interleukin-6, cytokine-induced neutrophil chemoattractant, and macrophage inflammatory protein 2 production and attenuated recruitment of polymorphonuclear leukocytes to the site of infection, indicating that acute inflammation was promoted by LBP. Locally produced LBP is an essential component of an effective innate immune response to E. coli peritonitis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Knapp S,de Vos AF,Florquin S,Golenbock DT,van der Poll Tdoi
10.1128/iai.71.12.6747-6753.2003keywords:
subject
Has Abstractpub_date
2003-12-01 00:00:00pages
6747-53issue
12eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::Helicobacter pylori mutants devoid of urease activity fail to colonize the gastric mucosa of mice; however, the effect of decreased levels of urease on colonization has not been examined. The nixA gene, required for full urease activity, encodes a cytoplasmic membrane nickel transporter that imports nickel ions and le...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.685-691.2002
更新日期:2002-02-01 00:00:00
abstract::The type-specific antigens of group B type Ic (old designation type Ii) streptococci were extracted, purified, and characterized by serological and chemical methods. The Ia antigen, shared by types Ia and Ic, is a polysaccharide composed of 69% galactose and 25% glucosamine (i.e., 31% N-acetyl-glucosamine). However, t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.596-604.1971
更新日期:1971-11-01 00:00:00
abstract::In the present study, we have investigated the evolution and impact on virulence of a 350-kb genomic duplication present in the most recently evolved members of the Mycobacterium tuberculosis East Asian lineage. In a mouse model of infection, comparing HN878 subclones HN878-27 (no duplication) and HN878-45 (with the 3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01791-14
更新日期:2014-07-01 00:00:00
abstract::Iron is limiting in the human host, and bacterial pathogens respond to this environment by regulating gene expression through the ferric uptake regulator protein (Fur). In vitro studies have demonstrated that Neisseria gonorrhoeae controls the expression of several critical genes through an iron- and Fur-mediated mech...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4281-4287.2005
更新日期:2005-07-01 00:00:00
abstract::Borrelia burgdorferi, the Lyme disease pathogen, dramatically alters its protein profile when it is transmitted between ticks and mammals. Several differentially expressed proteins have been shown to be critical for the enzootic cycle of B. burgdorferi. In this study, we demonstrated that expression of the surface lip...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00372-10
更新日期:2010-07-01 00:00:00
abstract::Patients with chronic mucocutaneous candidiasis (CMC) are selectively unable to clear the yeast Candida, which results in persistent debilitating infections affecting the skin, nails, and mucous membranes. The underlying defect is unknown. Recent animal studies highlighted the importance of type 1 cytokines in protect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5690-5699.2003
更新日期:2003-10-01 00:00:00
abstract::Autoclaving cell wall of Streptococcus mutans Ingbritt for 15 min under the Rantz and Randall conditions released one-tenth of the total cell wall carbohydrate, whereas two-thirds was extracted after autoclaving for 180 min. The extract contained the serotype c-specific antigen but lacked the lipoteichoic acid compone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.842-851.1978
更新日期:1978-12-01 00:00:00
abstract::An effective vaccine for Vibrio cholerae is not yet available for use in the developing world, where the burden of cholera disease is highest. Characterizing the proteins that are expressed by V. cholerae in the human host environment may provide insight into the pathogenesis of cholera and assist with the development...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-08
更新日期:2008-09-01 00:00:00
abstract::The purpose of this study was to localize antigenic regions on the flagellin protein of Campylobacter jejuni 81116. This strain has two flagellin genes, flaA and flaB, which are 95% identical; only flaA seems to be expressed in motile C. jejuni 81116 cells. Fragments of flaA and flaB were cloned in the bacterial expre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.1100-1105.1991
更新日期:1991-03-01 00:00:00
abstract::Mucosa-associated invariant T (MAIT) cells are an innate-like T cell subset in mammals that recognize microbial vitamin B metabolites presented by the evolutionarily conserved major histocompatibility complex class I (MHC I)-related molecule, MR1. Emerging data suggest that MAIT cells may be an attractive target for v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00524-20
更新日期:2020-12-15 00:00:00
abstract::Mouse peritoneal macrophages were infected with varying numbers of Nocardia asteroides 10905, and the fate of the ingested organisms was determined by viable plate count (VPC), light microscopy (LM), immunofluorescent microscopy (IM), and electron microscopy (EM). The results obtained with these methods differed. VPC ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.576-590.1974
更新日期:1974-03-01 00:00:00
abstract::The gastric spirillum Helicobacter felis, originally isolated from the cat stomach, colonizes the stomachs of germfree rats. Studies were designed to examine the pathological and serological responses of germfree rats inoculated orally with H. felis. At 2 weeks postinoculation, the gastric mucosa of germfree rats had ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.785-791.1991
更新日期:1991-03-01 00:00:00
abstract::We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and Palo-Alto(FUP)1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::C-reactive protein (CRP) has long been known to appear in the sera of individuals with inflammatory processes, but its role in host defense against bacterial infection is unclear. We have recently demonstrated that CRP in the presence of the classical complement pathway markedly enhances the opsonization of Streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.987-992.1982
更新日期:1982-09-01 00:00:00
abstract::The pathogenicities of three proteases from Serratia marcescens, two proteases from Pseudomonas aeruginosa, and one thermolysin from Bacillus stearothermophilus were examined. All proteases tested caused acute liquefactive necrosis of the cornea and descemetocele formation in guinea pig eyes after intrastromal injecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2509-2517.1987
更新日期:1987-10-01 00:00:00
abstract::A murine monoclonal antibody was raised against the B95-8 strain of Epstein-Barr virus (EBV), which was isolated from a case of mononucleosis after blood transfusion (Hoffman et al. Proc. Natl. Acad. Sci. U.S.A. 77:2979, 1980). We provide evidence that neutralization of immortalization by this monoclonal antibody is v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1028-1031.1982
更新日期:1982-09-01 00:00:00
abstract::Bartonella henselae is a recently recognized pathogenic bacterium associated with cat scratch disease, bacillary angiomatosis, and bacillary peliosis. This study describes the cloning, sequencing, and characterization of an antigenic autotransporter gene from B. henselae. A cloned 6.0-kb BclI-EcoRI DNA fragment expres...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-07
更新日期:2007-11-01 00:00:00
abstract::A zone of extracellular digestion of the mucin layer around Candida albicans blastoconidia was observed by transmission electron microscopy in the jejunum of mice inoculated intragastrically (G. T. Cole, K. R. Seshan, L. M. Pope, and R. J. Yancey, J. Med. Vet. Mycol. 26:173-185, 1988). This observation prompted the hy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4514-4519.1996
更新日期:1996-11-01 00:00:00
abstract::Exogenous ammonium ions (NH(4) (+)) and amine compounds had a profound influence on the antibacterial activity of the myeloperoxidase-hydrogen peroxide-chloride system against Escherichia coli. The rate of killing increased in the presence of NH(4) (+) and certain guanidino compounds and decreased in the presence of a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.110-116.1979
更新日期:1979-07-01 00:00:00
abstract::In order to evaluate during experimental Trypanosoma brucei infections the potential role of tumor necrosis factor alpha (TNF-alpha) in the host-parasite interrelationship, C57BL/6 TNF-alpha knockout mice (TNF-alpha-/-) as well as C57BL/6 wild-type mice were infected with pleomorphic T. brucei AnTat 1.1 E parasites. I...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.3128-3132.1999
更新日期:1999-06-01 00:00:00
abstract::The hydrolysis of urea by ureases of oral bacteria in dental plaque can cause a considerable increase in plaque pH, which can inhibit the development of dental caries. There is also indirect evidence that urea metabolism may promote the formation of calculus and that ammonia release from urea could exacerbate periodon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6670-6676.2000
更新日期:2000-12-01 00:00:00
abstract::Cells of Yersinia pestis strain EV76 are known to cease growth after a shift from 26 to 37 degrees C in neutral Ca2+-deficient medium; this effect is potentiated by Mg2+. With 2.5 mM Mg2+ and no added Ca2+, restriction was relaxed at elevated pH at which maximum cell yields occurred in the presence of 20 mM exogenous ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.953-959.1982
更新日期:1982-12-01 00:00:00
abstract::The effect of infection with low-virulence, tissue culture-propagated strains of reticuloendotheliosis virus (REV) on protective vaccinal immunity against Marek's disease (MD) lymphomas was investigated. Vaccinated chickens inoculated at hatching with greater than 10(4) focus-forming units of REV and challenged with M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.90-98.1979
更新日期:1979-10-01 00:00:00
abstract::Nasopharyngeal colonization represents the initial interaction between Haemophilus influenzae and its human host. Factors that influence bacterial carriage likely affect transmission and incidence of infection. Therefore, we investigated host factors involved in limiting H. influenzae colonization in BALB/c mice, as c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01564-08
更新日期:2009-08-01 00:00:00
abstract::Recruitment of neutrophils to lung tissue and airspaces is a hallmark of inflammatory events following inhalation of endotoxins. We studied the role of different lymphocyte subsets in this inflammation, which is assumed to primarily involve the innate immune system. Inhalation of aerosolized Escherichia coli lipopolys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6962-6969.2000
更新日期:2000-12-01 00:00:00
abstract::The involvement of tumor necrosis factor (TNF) in resistance to Toxoplasma gondii infection was examined by means of experiments in which mice were treated with anti-TNF antibodies prior to infection with ME49, a low-virulence Toxoplasma strain. In (BALB/cBy x C57BL/6J)F1 (CB6F1) mice, which are highly resistant to in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.1979-1983.1992
更新日期:1992-05-01 00:00:00
abstract::Previous studies have noted that the prevalence of certain gonococcal colony types is influenced by the physiological state of the human host. Consequently eight different gonococcal strains were passed on clear typing medium, and opaque (Op) and transparent (Tr) variants of heavily pilated (P++) bacteria were selecte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.359-364.1978
更新日期:1978-11-01 00:00:00
abstract::The antigenic relationship of six different strains of Paracoccidioides brasiliensis was investigated by the agar-gel immunodiffusion test. Soluble antigens prepared from the two morphological phases of the fungus were diffused against human sera obtained from eight patients with paracoccidioidomycosis (South American...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.3.268-273.1970
更新日期:1970-09-01 00:00:00
abstract::Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2285-2288.1994
更新日期:1994-06-01 00:00:00
abstract::Previous studies have demonstrated antigenic differences among the pili expressed by various strains of Haemophilus influenzae type b (Hib). In order to understand the molecular basis for these differences, the structural gene for pilin was cloned from Hib strain Eagan (p+) and the nucleotide sequence was compared to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1991-1996.1991
更新日期:1991-06-01 00:00:00