Abstract:
:Borrelia burgdorferi, the Lyme disease pathogen, dramatically alters its protein profile when it is transmitted between ticks and mammals. Several differentially expressed proteins have been shown to be critical for the enzootic cycle of B. burgdorferi. In this study, we demonstrated that expression of the surface lipoprotein-encoding gene bba07 is induced by an elevated temperature and a reduced pH during in vitro cultivation, as well as during nymphal tick feeding. Expression of bba07 is regulated by the Rrp2-RpoN-RpoS pathway, a central regulatory network that is activated during nymphal feeding. By generating a bba07 mutant of an infectious strain of B. burgdorferi, we demonstrated that although BBA07-deficient spirochetes were capable of infecting mice via needle inoculation and surviving in ticks, they were defective in infection of mammals via tick transmission. Complementation of the bba07 mutant with a wild-type copy of bba07 partially restored the transmission defect of the bba07 mutant. Based on these findings, we concluded that the surface lipoprotein BBA07 is produced during tick feeding and facilitates optimal transmission of B. burgdorferi from the tick vector to a mammalian host.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Xu H,He M,He JJ,Yang XFdoi
10.1128/IAI.00372-10subject
Has Abstractpub_date
2010-07-01 00:00:00pages
2910-8issue
7eissn
0019-9567issn
1098-5522pii
IAI.00372-10journal_volume
78pub_type
杂志文章abstract::Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections. These bacteria undertake a multistage infection cycle involving invasion of and proliferation within urinary tract epithelial cells, leading to the rupture of the host cell and dispersal of the bacteria, some of which have a highly...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00884-19
更新日期:2020-08-19 00:00:00
abstract::Immunologically competent mice and mice with defined immunological deficiencies were infected with Plasmodium yoelii. Splenomegaly, enhanced phagocytosis, and anemia were most marked in infected mice having intact thymic tissue. Whereas the spleens of infected nude mice increased minimally in size, the relative blood ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.728-731.1978
更新日期:1978-06-01 00:00:00
abstract::Mice (BALB/cJ, C3H/HeN, and C3H/HeJ) primed with actinomycin D became highly susceptible to lethal intoxication with staphylococcal enterotoxin B (SEB). The mice underwent toxicosis and toxic shock and died. Actinomycin D-primed C3H/HeN and C3H/HeJ mice showed equal sensitivity to SEB, suggesting that bacterial lipopo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.10.4626-4631.1994
更新日期:1994-10-01 00:00:00
abstract::Several immunological, hematological, and pathological responses associated with Marek's disease were determined. Four-week-old Marek's disease-infected and control chickens were injected with Salmonella pullorum antigen. About one-half of all infected chickens tested were unresponsive to antigenic challenge. Antibody...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.567-574.1971
更新日期:1971-11-01 00:00:00
abstract::Escherichia coli is a major cause of enteric/diarrheal diseases, urinary tract infections, and sepsis. E. coli K1 is the leading gram-negative organism causing neonatal meningitis, but the microbial basis of E. coli K1 meningitis is incompletely understood. Here we employed comparative genomic hybridization to investi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2196-2206.2006
更新日期:2006-04-01 00:00:00
abstract::Streptococcus pneumoniae cell wall and pneumolysin are important contributors to pneumococcal pathogenicity in some animal models. To further explore these factors in middle ear inflammation caused by pneumococci, penicillin-induced inflammatory acceleration was studied by using three closely related pneumococcal stra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1140-1145.1996
更新日期:1996-04-01 00:00:00
abstract::Q fever has been associated with granulomatous changes in clinical biopsy material obtained from liver and bone marrow. Local reactions to skin testing have been described in previously sensitized humans, but histological studies of such reactions have not been reported. We note that delayed hypersensitivity reactions...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.388-393.1983
更新日期:1983-01-01 00:00:00
abstract::A major obstacle to studying the functions of particular gene products in the mouse-tick infectious cycle of Borrelia burgdorferi has been an inability to knock out genes in pathogenic strains. Here, we investigated conditions for site-directed mutagenesis in B31 MI, the low-passage-number, infectious B. burgdorferi s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2139-2150.2002
更新日期:2002-04-01 00:00:00
abstract::In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 microg of Vi and induced a greater-than-eightfold rise in immunoglobulin G (IgG) anti-Vi in all of the vaccinees tested. In this investigati...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.72.11.6586-6588.2004
更新日期:2004-11-01 00:00:00
abstract::Previous studies in our laboratory have indicated that naturally resistant, inbred DBA/2J mice mount a greater serum antibody response to Pseudomonas aeruginosa 19660 than susceptible C57BL/6J mice. However, the specificity of the antibody produced was not known. The present study examines the specificity and kinetics...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1984-1990.1991
更新日期:1991-06-01 00:00:00
abstract::Chlamydial inclusions were demonstrated by indirect immunofluorescence (IF) with antiserum to the chlamydial group antigen when McCoy cell monolayers infected with either Chlamydia trachomatis or Chlamydia psittaci were fixed in formaldehyde or paraformaldehyde, provided the monolayer was not allowed to dry. If these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.561-570.1981
更新日期:1981-11-01 00:00:00
abstract::Complement receptor type 3 (CR3) was initially described as an opsonic receptor. Subsequently, CR3-mediated lectin-sugar recognition mechanisms have been shown to play a major role in the nonopsonic phagocytosis of several pathogens, among them Mycobacterium tuberculosis. Little is known about the binding and signal t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4736-4745.2000
更新日期:2000-08-01 00:00:00
abstract::Antibodies against the Plasmodium falciparum P0 ribosomal phosphoprotein (PfP0) have been detected exclusively but extensively in malaria-immune persons. Polyclonal rabbit and mice sera were raised against two recombinant polypeptides of P. falciparum P0 protein, PfP0N and PfP0C, covering amino acids 17 to 61 and the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4312-4318.2000
更新日期:2000-07-01 00:00:00
abstract::Clostridium perfringens enterotoxin (CPE), a single polypeptide of 319 amino acids, has a unique multistep mechanism of action. In the first step, CPE binds to claudin proteins and/or a 50-kDa eukaryotic membrane protein receptor, forming a small ( approximately 90-kDa) complex. This small complex apparently then asso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.5634-5641.1999
更新日期:1999-11-01 00:00:00
abstract::Ehrlichia risticii incubated with mouse peritoneal macrophages elicited with thioglycolate broth survived and replicated, thereby allowing examination of the effects of several immunopotentiating agents. Treatment of the macrophages with recombinant murine gamma interferon (rMuIFN-gamma) in vitro at 1 day before or 3 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3418-3423.1991
更新日期:1991-10-01 00:00:00
abstract::Cord factor (trehalose 6,6'-dimycolate[TDM] ), a mixture of 6,6'-diesters of alpha, alpha-D-trehalose with natural mycolic acids, has been described as having immunoregulatory and antitumor activities in vivo, although the relevant mechanisms of action remain unelucidated. In this work, we measured the effects of TDM ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.531-535.1984
更新日期:1984-02-01 00:00:00
abstract::Genetic diversity and relationships among 109 isolates of Neisseria meningitidis obtained from throat cultures of healthy individuals in Norway in 1984 were assessed by analyzing electrophoretically demonstrable allelic variation at 15 enzyme-encoding chromosomal genes. Seventy-eight distinctive electrophoretic types ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2060-2068.1988
更新日期:1988-08-01 00:00:00
abstract::Bactericidal activity and binding of a 57,000-dalton cationic antimicrobial neutrophil granule protein (CAP57) are determined by the presence on bacteria of O-antigen polysaccharide chains and the availability of negatively charged groups in the lipid A region, the inner core region, or both regions of lipopolysacchar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1589-1592.1988
更新日期:1988-06-01 00:00:00
abstract::In order to approximate and adhere to mucosal epithelial cells, Candida must traverse the overlying mucus layer. Interactions of Candida species with mucin and human buccal epithelial cells (BECs) were thus investigated in vitro. Binding of the Candida species to purified small intestinal mucin showed a close correlat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3172-3179.2000
更新日期:2000-06-01 00:00:00
abstract::Tuberculosis continues to ravage humanity, killing 2 million people yearly. Most cases occur in areas of the world to which the disease is endemic, where almost everyone is vaccinated early in life with Mycobacterium bovis BCG, the currently available vaccine against tuberculosis. Thus, while more-potent vaccines are ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4676-4683.2005
更新日期:2005-08-01 00:00:00
abstract::Alveolar bone destruction is a characteristic feature of periodontitis. Treponema denticola is known to be involved in periodontitis. To elucidate the role of T. denticola in alveolar bone destruction in periodontitis, the effects of lipooligosaccharide (LOS) from T. denticola on osteoclast formation and on expression...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.226-233.2003
更新日期:2003-01-01 00:00:00
abstract::The effect of the secondary infection with Toxoplasma gondii on antibody responses to unrelated antigens was examined in mice. A reinfection with 5 X 10(3) organisms did not affect either primary anti-sheep erythrocyte antibody responses, or anti-dinitrophenol antibody responses to dinitrophenylated keyhole limpet hem...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.686-689.1985
更新日期:1985-06-01 00:00:00
abstract::We have investigated the ability of two species of streptococci isolated from the human oral cavity (Streptococcus mutans NCTC 10449 and Streptococcus sobrinus SL-1) to metabolize N-acetylglucosamine (GlcNAc), a naturally occurring amino sugar present in saliva and human glycoproteins, when provided as the sole fermen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.295-302.1993
更新日期:1993-01-01 00:00:00
abstract::Brucella abortus injected into CBA mice replicated primarily in the spleen and liver, reaching a peak bacterial count in both organs about 7 days postinfection. The organism was eliminated from the liver but declined to a chronic phase in the spleen. The infection caused hepatosplenomegaly. An influx of macrophages in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1465-1472.1992
更新日期:1992-04-01 00:00:00
abstract::Vibrio parahaemolyticus is a gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. Almost all of the clinical V. parahaemolyticus isolates exhibit beta-type hemolysis on Wagatsuma agar, known as the Kanagawa phenomenon (KP). KP is induced by the thermostable dire...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01535-07
更新日期:2008-03-01 00:00:00
abstract::The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1424-1431.1999
更新日期:1999-03-01 00:00:00
abstract::Bacterial tyrosine kinases and their cognate protein tyrosine phosphatases are best known for regulating the biosynthesis of polysaccharides. Moreover, their roles in the stress response, DNA metabolism, cell division, and virulence have also been documented. The aim of this study was to investigate the pathogenicity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02713-14
更新日期:2015-02-01 00:00:00
abstract::We studied the antigenicity of a wild-type flagellate and motile (F+M+) Campylobacter jejuni strain (81116) and two daughter mutants, one flagellate and immotile (F+M-) and one aflagellate and immotile (F-M-). By sodium dodecyl sulfate-polyacrylamide gel electrophoresis of acid-extracted surface proteins, a 63-kilodal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::CXCL16 is a recently discovered multifaceted chemokine that has been shown not only to recruit activated T lymphocytes but also to play a direct role in the binding and phagocytosis of bacteria by professional antigen-presenting cells. In this study, we investigated the role of CXCL16 in vivo in the regulation of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01065-06
更新日期:2006-12-01 00:00:00
abstract::In order to plan for the wide-scale introduction of meningococcal C conjugate (MCC) vaccine for United Kingdom children up to 18 years old, phase II trials were undertaken to investigate whether there was any interaction between MCC vaccines conjugated to tetanus toxoid (TT) or a derivative of diphtheria toxin (CRM(19...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/iai.70.9.4946-4954.2002
更新日期:2002-09-01 00:00:00