Roles of autolysin and pneumolysin in middle ear inflammation caused by a type 3 Streptococcus pneumoniae strain in the chinchilla otitis media model.


:Streptococcus pneumoniae cell wall and pneumolysin are important contributors to pneumococcal pathogenicity in some animal models. To further explore these factors in middle ear inflammation caused by pneumococci, penicillin-induced inflammatory acceleration was studied by using three closely related pneumococcal strains: a wild-type 3 strain (WT3), its pneumolysin-negative derivative (P-1), and into autolysin-negative derivative (A-1). Both middle ears of chinchillas were inoculated with one of the three pneumococcal strains. During the first 12 h, all three strains grew in vivo at the same rate, and all three strains induced similar inflammatory cell responses in middle ear fluid (MEF). Procaine penicillin G was given as 12 h to one-half of the animals in each group, and all treated chinchillas had sterile MEF at 24 h. Penicillin significantly accelerated MEF inflammatory cell influx into WT3-and P-1-infected ears at 18 and 24 h in comparison with the rate for penicillin-treated A-1-infected ears. Inflammatory cell influx was slightly, but not significantly, greater after treatment of WT3 infection than after treatment of P-1 infection. Interleukin (IL)-1beta and IL-6, but not IL-8, concentrations in MEF at 24 h reflected the penicillin effect on MEF inflammatory cells; however, differences between treatment groups were not significant. Results suggest that pneumococcal otitis media pathogenesis is triggered principally by the inflammatory effects of intact and lytic cell wall products in the middle ear, with at most a modes additional pneumolysin effect. Investigation strategies that limit the release of these products or neutralize them warrant further investigation.


Infect Immun


Infection and immunity


Sato K,Quartey MK,Liebeler CL,Le CT,Giebink GS




Has Abstract


1996-04-01 00:00:00












  • Characterization of antigens from the yeast phase of Histoplasma capsulatum.

    abstract::Preliminary studies established methods for obtaining maximum yields of viable cells. Liquid shaken cultures of Histoplasma capsulatum provided a maximum of 4 x 10(8) to 5 x 10(8) cells/ml regardless of the inoculum size. Under optimum conditions, cells were viable (90 to 95%) for 168 to 240 hr. Generation times range...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Garcia JP,Howard DH

    更新日期:1971-08-01 00:00:00

  • Vibrio cholerae expresses cell surface antigens during intestinal infection which are not expressed during in vitro culture.

    abstract::Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env) proteins that were not found on vibrios after in vitro culture in various ordinary liquid media. At the same time, several of t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jonson G,Svennerholm AM,Holmgren J

    更新日期:1989-06-01 00:00:00

  • Cellular source of interferons in the circulation of mice with delayed hypersensitivity.

    abstract::The cellular origins of type I and type II interferons released into the circulation of mice with delayed hypersensitivity were investigated. We determined the effect of treatment with various immunosuppressive agents, including cyclophosphamide, cycloheximide, antithymocyte serum, and whole-body X-irradiation, on the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sonnenfeld G,Salvin SB,Youngner JS

    更新日期:1977-11-01 00:00:00

  • The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori.

    abstract::The NikR protein is a nickel-dependent regulatory protein which is a member of the ribbon-helix-helix family of transcriptional regulators. The gastric pathogen Helicobacter pylori expresses a NikR ortholog, which was previously shown to mediate regulation of metal metabolism and urease expression, but the mechanism g...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ernst FD,Kuipers EJ,Heijens A,Sarwari R,Stoof J,Penn CW,Kusters JG,van Vliet AH

    更新日期:2005-11-01 00:00:00

  • Gonococcal nitric oxide reductase is encoded by a single gene, norB, which is required for anaerobic growth and is induced by nitric oxide.

    abstract::The gene encoding a nitric oxide reductase has been identified in Neisseria gonorrhoeae. The norB gene product shares significant identity with the nitric oxide reductases in Ralstonia eutropha and Synechocystis sp. and, like those organisms, the gonococcus lacks a norC homolog. The gonococcal norB gene was found to b...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Householder TC,Fozo EM,Cardinale JA,Clark VL

    更新日期:2000-09-01 00:00:00

  • Trypanosoma rhodesiense infection in congenitally athymic (nude) mice.

    abstract::Athymic nude mice (nu/nu), heterozygous litter mates (nu/+), and thymic reconstituted homozygous animals (nu/nu TxR) were infected with Trypanosoma rhodesiense. A reduced parasitemia and prolonged survival were observed in the nu/nu animals after infection and cure or after immunization with irradiated organisms. Thes...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Campbell GH,Esser KM,Phillips SM

    更新日期:1978-06-01 00:00:00

  • Antitoxic immunity in experimental cholera: observations with purified antigens and the rat foot edema model.

    abstract::The recently introduced choleragen-induced rat foot edema model has been employed as a bioassay for evaluating the immunogenicity of three purified preparations containing cholera exo-enterotoxin antigen, choleragen, choleragenoid, and Formalin-treated choleragen (formagen). The results indicated that choleragen evoke...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Finkelstein RA,Hollingsworth RC

    更新日期:1970-05-01 00:00:00

  • A siderophore production mutant of Bordetella bronchiseptica cannot use lactoferrin as an iron source.

    abstract::Bordetella bronchiseptica secreted a hydroxamate siderophore when grown in Fe-depleted medium. A Tn5lac insertion mutant of B. bronchiseptica, DBB22, did not produce this hydroxamate siderophore and was incapable of using lactoferrin as an Fe source. Our data suggest that B. bronchiseptica uses a siderophore for remov...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Foster LA,Dyer DW

    更新日期:1993-06-01 00:00:00

  • Gram-positive cell walls stimulate synthesis of tumor necrosis factor alpha and interleukin-6 by human monocytes.

    abstract::Purified cell walls representing a wide variety in teichoic acid and peptidoglycan structure prepared from eight different gram-positive bacterial species induced the production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 from human monocytes in the presence of 10% plasma or serum. Significant amounts...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Heumann D,Barras C,Severin A,Glauser MP,Tomasz A

    更新日期:1994-07-01 00:00:00

  • Rickettsia rickettsii infection of cultured human endothelial cells induces heme oxygenase 1 expression.

    abstract::Existing evidence suggests that oxidative insults and antioxidant defense mechanisms play a critical role in the host cell response during infection of endothelial cells by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever. Heme oxygenase (HO), a rate-limiting enzyme in the pathway for heme ca...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rydkina E,Sahni A,Silverman DJ,Sahni SK

    更新日期:2002-08-01 00:00:00

  • Critical Role of Zinc in a New Murine Model of Enterotoxigenic Escherichia coli Diarrhea.

    abstract::Enterotoxigenic Escherichia coli (ETEC) is a major cause of traveler's diarrhea as well as of endemic diarrhea and stunting in children in developing areas. However, a small-mammal model has been badly needed to better understand and assess mechanisms, vaccines, and interventions. We report a murine model of ETEC diar...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bolick DT,Medeiros PHQS,Ledwaba SE,Lima AAM,Nataro JP,Barry EM,Guerrant RL

    更新日期:2018-06-21 00:00:00

  • Antigens of Bordetella pertussis V. Separation of Agglutinogen 1 and Mouse-Protective Antigen.

    abstract::Agglutinogen 1 of Bordetella pertussis strain 353/Z (serotype 1) was separated from protective antigen and histamine-sensitizing factor by starch-block electrophoresis. Most of the agglutinogen 1 migrated towards the cathode in starch-block electrophoresis, although some remained near the origin. Fractions containing ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ross RF,Munoz J

    更新日期:1971-02-01 00:00:00

  • Peptidoglycan and lipoteichoic acid from Staphylococcus aureus induce tumor necrosis factor alpha, interleukin 6 (IL-6), and IL-10 production in both T cells and monocytes in a human whole blood model.

    abstract::We have examined the ability of peptidoglycan (PepG) and lipoteichoic acid (LTA) isolated from Staphylococcus aureus to induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and IL-10 in whole human blood and identified the cellular origins of these cytokines. Both PepG and LTA induced t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Wang JE,Jørgensen PF,Almlöf M,Thiemermann C,Foster SJ,Aasen AO,Solberg R

    更新日期:2000-07-01 00:00:00

  • Cloning of the gene encoding the major outer membrane protein of Haemophilus influenzae type b.

    abstract::The major outer membrane protein (P2) of Haemophilus influenzae type b (Hib) with an apparent molecular weight of 37,000 to 40,000 has been previously shown to function as a porin and also as a target for antibodies protective against experimental Hib disease. The gene encoding the Hib P2 protein was cloned by using a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hansen EJ,Gonzales FR,Chamberlain NR,Norgard MV,Miller EE,Cope LD,Pelzel SE,Gaddy B,Clausell A

    更新日期:1988-10-01 00:00:00

  • Antigen capture of Porphyromonas gingivalis by human macrophages is enhanced but killing and antigen presentation are reduced by endotoxin tolerance.

    abstract::The innate and the adaptive arms of the mucosal immune system must be coordinated to facilitate the control of pathogenic invasion while maintaining immune homeostasis. Toll-like receptors, able to activate the cell to produce bactericidal and inflammatory cytokines but also able to upregulate antigen (Ag)-presenting ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Muthukuru M,Cutler CW

    更新日期:2008-02-01 00:00:00

  • Ferredoxin is involved in secretion of cytotoxic necrotizing factor 1 across the cytoplasmic membrane in Escherichia coli K1.

    abstract::We previously showed that cytotoxic necrotizing factor 1 (CNF1) contributes to Escherichia coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and interacts with the receptor on the surface of HBMEC. CNF1 is the cytoplasmic protein, and it remains incompletely understood how CNF1 is secreted across...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yu H,Kim KS

    更新日期:2010-02-01 00:00:00

  • Virulence factors determine attachment and ingestion of nonopsonized and opsonized Bordetella pertussis by human monocytes.

    abstract::In the present study, the role of virulence factors in and the effect of opsonization on the interactions between Bordetella pertussis and human monocytes were investigated. The methods used facilitated the distinction between attachment and ingestion of bacteria by monocytes. Nonopsonized virulent B. pertussis cells ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hazenbos WL,van den Berg BM,van't Wout JW,Mooi FR,van Furth R

    更新日期:1994-11-01 00:00:00

  • Human monocytes promote Th1 and Th17 responses to Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae is a leading cause of bacterial pneumonia, meningitis, and sepsis in children. Human immunity to pneumococcal infections has been assumed to depend on anticapsular antibodies. However, recent findings from murine models suggest that alternative mechanisms, dependent on T helper cells, are also...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Olliver M,Hiew J,Mellroth P,Henriques-Normark B,Bergman P

    更新日期:2011-10-01 00:00:00

  • Hepatitis B surface antigen-specific cell-mediated immune responses in human chronic hepatitis B surface antigen carriers.

    abstract::The presence of hepatitis B surface antigen (HBsAg) and antibody (anti-HBs), hepatitis B e antigen (HBeAg) and antibody (anti-HBe), the nature of T-cell function, and specific cell-mediated immunity to HBsAg were determined and evaluated serially in groups of subjects with chronic HBsAg carrier states and in seronegat...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Beutner KR,Tiku ML,Ogra PL

    更新日期:1978-08-01 00:00:00

  • Efficacy of the 26-kilodalton outer membrane protein and two P5 fimbrin-derived immunogens to induce clearance of nontypeable Haemophilus influenzae from the rat middle ear and lungs as well as from the chinchilla middle ear and nasopharynx.

    abstract::The rat middle ear and lung clearance model has been used to show that the nontypeable Haemophilus influenzae 26-kDa outer membrane protein OMP26 is highly efficacious as a mucosal immunogen, inducing significantly enhanced clearance in immunized rats upon direct challenge of these two anatomic sites. Similarly, the c...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kyd JM,Cripps AW,Novotny LA,Bakaletz LO

    更新日期:2003-08-01 00:00:00

  • Requirements for assembly of PtlH with the pertussis toxin transporter apparatus of Bordetella pertussis.

    abstract::PtlH is an essential component of the Ptl system, the type IV transporter responsible for secretion of pertussis toxin (PT) across the outer membrane of Bordetella pertussis. The nine Ptl proteins are believed to interact to form a membrane-spanning apparatus through which the toxin is secreted. In this study, we moni...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Verma A,Burns DL

    更新日期:2007-05-01 00:00:00

  • Nontypeable Haemophilus influenzae gene expression induced in vivo in a chinchilla model of otitis media.

    abstract::The gram-negative bacterium nontypeable Haemophilus influenzae (NTHI) is the predominant pathogen in chronic otitis media with effusion and, with Streptococcus pneumoniae and Moraxella catarrhalis, is a causative agent of acute otitis media. To identify potential virulence determinants, bacterial gene expression was m...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mason KM,Munson RS Jr,Bakaletz LO

    更新日期:2003-06-01 00:00:00

  • Identification of abundantly expressed novel and conserved genes from the infective larval stage of Toxocara canis by an expressed sequence tag strategy.

    abstract::Larvae of Toxocara canis, a nematode parasite of dogs, infect humans, causing visceral and ocular larva migrans. In noncanid hosts, larvae neither grow nor differentiate but endure in a state of arrested development. Reasoning that parasite protein production is orientated to immune evasion, we undertook a random sequ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tetteh KK,Loukas A,Tripp C,Maizels RM

    更新日期:1999-09-01 00:00:00

  • Intracellular growth and cytotoxicity of Mycobacterium haemophilum in a human epithelial cell line (Hec-1-B).

    abstract::We developed an in vitro model to study the temperature-regulated cytotoxicity and intracellular growth of Mycobacterium haemophilum in cultured human epithelial and endothelial cells. M. haemophilum associated with human epithelial and endothelial cells at similar rates when incubated at 33 and 37 degrees C, but only...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fischer LJ,Quinn FD,White EH,King CH

    更新日期:1996-01-01 00:00:00

  • Ultrastructural study of adherence to and penetration of cultured cells by two invasive Escherichia coli strains isolated from infants with enteritis.

    abstract::The adherence of invasive Escherichia coli strains 444-3 and 469-3 to human erythrocytes and to cultured HeLa and HEp-2 cells has been examined by electron microscopy. Bacteria elaborating type 1 fimbriae, glycocalyces , and nonfimbrial mannose-resistant hemagglutinins specific for human erythrocytes were identified i...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Knutton S,Williams PH,Lloyd DR,Candy DC,McNeish AS

    更新日期:1984-06-01 00:00:00

  • Binding of colloidal gold-labeled salivary proline-rich proteins to Actinomyces viscosus type 1 fimbriae.

    abstract::Salivary proline-rich proteins (PRPs), which were purified from parotid saliva, were adsorbed onto 15-nm-diameter gold particles to visualize specific binding of the salivary molecules to Actinomyces viscosus type 1 fimbriae. Negatively stained preparations incubated with PRP-gold conjugates but not bovine serum album...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Leung KP,Nesbitt WE,Fischlschweiger W,Hay DI,Clark WB

    更新日期:1990-06-01 00:00:00

  • Regulatory T Cells Contribute to Resistance against Lyme Arthritis.

    abstract::The symptoms of Lyme disease are caused by inflammation induced by species of the Borrelia burgdorferisensu lato complex. The various presentations of Lyme disease in the population suggest that differences exist in the intensity and regulation of the host response to the spirochete. Previous work has described correl...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Siebers EM,Liedhegner ES,Lawlor MW,Schell RF,Nardelli DT

    更新日期:2020-10-19 00:00:00

  • Differential role of CbpA and PspA in modulation of in vitro CXC chemokine responses of respiratory epithelial cells to infection with Streptococcus pneumoniae.

    abstract::Respiratory epithelial cells play an active part in the host response to respiratory pathogens, such as Streptococcus pneumoniae, by releasing chemokines responsible for neutrophil recruitment. In order to investigate the role of specific pneumococcal virulence factors in eliciting CXC chemokine responses, type II pne...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Graham RM,Paton JC

    更新日期:2006-12-01 00:00:00

  • Protection against colibacillosis in neonatal piglets by immunization of dams with procholeragenoid.

    abstract::Protection against colibacillosis in neonatal piglets was obtained by immunization of pregnant dams with procholeragenoid. Procholeragenoid is a stable high-molecular-weight aggregate of cholera toxin formed during the heating of cholera toxin. Procholeragenoid retained approximately 1% of the toxicity of native toxin...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fürer E,Cryz SJ Jr,Dorner F,Nicolet J,Wanner M,Germanier R

    更新日期:1982-03-01 00:00:00

  • Type I interferons promote severe disease in a mouse model of lethal ehrlichiosis.

    abstract::Human monocytic ehrlichiosis (HME) is caused by a tick-borne obligate intracellular pathogen of the order Rickettsiales. HME disease can range from mild to a fatal, toxic shock-like syndrome, yet the mechanisms regulating pathogenesis are not well understood. We define a central role for type I interferons (alpha inte...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zhang Y,Thai V,McCabe A,Jones M,MacNamara KC

    更新日期:2014-04-01 00:00:00