Abstract:
:Alveolar bone destruction is a characteristic feature of periodontitis. Treponema denticola is known to be involved in periodontitis. To elucidate the role of T. denticola in alveolar bone destruction in periodontitis, the effects of lipooligosaccharide (LOS) from T. denticola on osteoclast formation and on expression of osteoclast differentiation factor (ODF) and osteoprotegerin (OPG) mRNAs were examined in a coculture system by using mouse calvaria and bone marrow cells. In addition, the effect of T. denticola LOS on expression of matrix metalloproteinases (MMPs), which are involved in bone resorption, was estimated in mouse calvaria-derived osteoblastic cells. When the mouse calvaria and bone marrow cells were challenged with LOS (0.1 to 10 micro g/ml) for 4 days, the number of tartrate-resistant acid phosphatase-positive multinucleated cells increased in a dose-dependent manner. The expression of ODF mRNA increased, while OPG mRNA expression decreased. Polymyxin B changed the effect of LOS (10 micro g/ml) on ODF and OPG mRNA expression to the control level. LOS (10 micro g/ml) stimulated prostaglandin E(2) (PGE(2)) production in the cocultures. Adding indomethacin, an inhibitor of prostaglandin synthesis, resulted in a reduction in the number of osteoclasts induced by LOS and eliminated the effect of T. denticola LOS on ODF and OPG mRNA expression. T. denticola LOS increased the levels of mRNAs encoding MMP-3, -8, -9, -10, -13, and -14. Expression of one of these mRNAs, MMP-9 mRNA, was significantly induced by T. denticola LOS. These findings suggest that LOS from T. denticola stimulates osteoclastogenesis and MMP expression. Up-regulation of ODF and down-regulation of OPG by a PGE(2)-dependent mechanism were involved in the osteoclastogenesis induced by T. denticola LOS.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Choi BK,Lee HJ,Kang JH,Jeong GJ,Min CK,Yoo YJdoi
10.1128/iai.71.1.226-233.2003keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
226-33issue
1eissn
0019-9567issn
1098-5522journal_volume
71pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1981-05-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1982-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1993-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1314-1321.1992
更新日期:1992-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-06-22 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00
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更新日期:2014-06-01 00:00:00
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更新日期:2002-02-01 00:00:00
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更新日期:2000-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1996-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1981-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00