Isolation of Bacteroides fragilis mutants with in vivo growth defects by using Tn4400', a modified Tn4400 transposition system, and a new screening method.

Abstract:

:A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich medium anaerobically.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Tang YP,Malamy MH

doi

10.1128/iai.68.1.415-419.2000

keywords:

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

415-9

issue

1

eissn

0019-9567

issn

1098-5522

journal_volume

68

pub_type

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