Abstract:
:A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich medium anaerobically.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tang YP,Malamy MHdoi
10.1128/iai.68.1.415-419.2000keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
415-9issue
1eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3380-3387.1990
更新日期:1990-10-01 00:00:00
abstract::Haemophilus influenzae type b (Hib) is a significant pathogen for young children, and three Hib vaccines (named PRP-OMPC, HbOC, and PRP-T) are currently available for young children. Extensive studies of anti-Hib polysaccharide (PS) antibodies (Abs) have shown that the V regions of Abs against the Hib PS comprise a VH...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.11.4219-4223.1995
更新日期:1995-11-01 00:00:00
abstract::Mice were infected with Chlamydia trachomatis (L2/434) and at various intervals after infection their lymphoid cells were tested directly for cytotoxic activity against Chlamydia-infected L929 cells. In a modified cell-mediated lympholysis assay, splenic, lymph node, and peritoneal exudate cells did not significantly ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1271-1274.1983
更新日期:1983-03-01 00:00:00
abstract::It is currently accepted that malaria-parasitized red blood cells (pRBC) are eliminated, like senescent erythrocytes, phagocytically by macrophages in the red pulp of the spleen. Here, however, we show that self-healing Plasmodium chabaudi malaria activates spleen closure in C57BL/6 mice. Confocal laser scanning micro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6390-6398.2005
更新日期:2005-10-01 00:00:00
abstract::Athymic BALB/c nude mice and euthymic BALB/c mice were infected with Rickettsia akari by the intraperitoneal route. The rickettsialpox infection was terminated in euthymic mice with only two intraperitoneal injections of the antibiotic oxytetracycline, whereas prolonged treatment was necessary to terminate the infecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::The primary antibody response of BALB/c splenocytes to sheep erythrocytes in vitro was suppressed by infection with Friend leukemia virus (FLV), with the response capacity decreasing with increasing duration of infection. The acquisition of normal antibody responses was amplified by macrophage-produced antibody respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1260-1264.1983
更新日期:1983-03-01 00:00:00
abstract::Serine proteases and serine protease homologs form the second largest gene family in the Drosophila melanogaster genome. Certain genes in the Jonah multigene family encoding serine proteases have been implicated in the fly antiviral immune response. Here, we report the involvement of Jonah66Ci in the Drosophila immune...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-19
更新日期:2019-08-21 00:00:00
abstract::Immunoglobulin class-specific antibodies were measured by a solid-phase radioimmunoassay in serum, bronchoalveolar washings (BAW), lung cell lysates, and spleen cell lysates in germfree mice after intranasal (i.n.) and intraperitoneal (i.p.) primary and secondary 10(5), 10(4), and 10(3) mean tissue culture infective d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.3.521-527.1977
更新日期:1977-09-01 00:00:00
abstract::The adherence and aggregation properties of 46 human oral Streptococcus salivarius isolates were examined. A total of 41% of the isolates aggregated with whole human saliva, 50% aggregated with human erythrocytes, and 85% adhered to human buccal epithelial cells. Strains that aggregated with saliva and erythrocytes us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Decay-accelerating factor (DAF), a complement regulatory protein, also serves as a receptor for Dr adhesin-bearing Escherichia coli. The repeat three of DAF was shown to be important in Dr adhesin binding and complement regulation. However, Dr adhesins do not bind to red blood cells with the rare polymorphism of DAF, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4485-4493.2002
更新日期:2002-08-01 00:00:00
abstract::Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incubation of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2115-2120.2006
更新日期:2006-04-01 00:00:00
abstract::An immunological assay utilizing double-diffusion principles was developed for identification of Pseudomonas aeruginosa mutants deficient in toxin A. Mutations were chemically induced, and mutants were isolated from P. aeruginosa strain PA103. Quantitative assays, both enzymatic and immunological, indicated that five ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::The immune mechanism by which hamsters acquire resistance to infection with Treponema pertenue, the causative agent of frambesia, or yaws, has not been elucidated. Serum or cells (spleen or lymph node) obtained from hamsters resistant to frambesial infection were transferred to normal syngenic recipients, who are subs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.430-435.1978
更新日期:1978-08-01 00:00:00
abstract::Plasmodium falciparum's ability to invade erythrocytes is essential for its survival within the human host. Immune mechanisms that impair this ability are therefore expected to contribute to immunity against the parasite. Plasma of humans who are naturally exposed to malaria has been shown to have growth-inhibitory ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06190-11
更新日期:2012-05-01 00:00:00
abstract::Peroxidase-conjugated antibodies were found to be as sensitive as those conjugated to fluorescein-isothiocyanate (FITC) for identification of selected enterovirus types by the indirect technique. Peroxidase conjugates, however, were found to give fewer nonspecific reactions. The reasons for the higher specificity of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.220-226.1974
更新日期:1974-07-01 00:00:00
abstract::Infection of L cells with Chlamydia psittaci inhibited the burst of deoxyribonucleic acid synthesis that occurs when the host cells are released from a double thymidine block. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.363-364.1971
更新日期:1971-02-01 00:00:00
abstract::Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability to inhibit the binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding to the repeating unit of the Pn14PS, a hexasaccharide mimic, and an octasaccharide frag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5107-5114.2002
更新日期:2002-09-01 00:00:00
abstract::Colony counts and light microscopy were used to assess the capacity of Candida albicans to colonize, infect the alimentary tract, and cause disseminated disease in athymic (nu/nu), euthymic (nu/+), beige (bg/bg), black (bg/+), beige athymic (bg/bg nu/nu), or beige euthymic (bg/bg nu/+) germfree mice. The alimentary tr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1093-1100.1990
更新日期:1990-04-01 00:00:00
abstract::Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.209-215.1982
更新日期:1982-07-01 00:00:00
abstract::A series of experiments was conducted to study the influence of embryonal bursectomy (EBX) on the early and late pathogenesis of Marek's disease (MD). The early lytic infection in the lymphoid organs normally associated with oncogenic MD virus infection in intact chickens was not seen in EBX chickens. Therefore, the d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.199-207.1981
更新日期:1981-01-01 00:00:00
abstract::The action of trehalose-6, 6'-dimycolate (cord factor) of Mycobacterium tuberculosis to induce site II-specific inhibition of mitochondrial oxidative phosphorylation and to stimulate adenosine triphosphatase activity was neutralized by its specific antibody. The activity was restored after dissociation of cord factor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.277-279.1974
更新日期:1974-07-01 00:00:00
abstract::The protozoan parasite Entamoeba histolytica causes amebic dysentery and amebic liver abscess, diseases associated with significant morbidity and mortality worldwide. E. histolytica infection appears to involve the initial attachment of amebic trophozoites to intestinal epithelial cells, followed by lysis of these cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1631-1639.1997
更新日期:1997-05-01 00:00:00
abstract::The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a signi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7170-7172.2003
更新日期:2003-12-01 00:00:00
abstract::We determined the diphtheria toxin phenotype of specially selected isolates of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (C. ovis). All produced proteins similar in size and immunological structure to diphtheria toxin. As with diphtheria toxin, they exhibited ADP-ribosylating activity, and their ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1114-1116.1984
更新日期:1984-03-01 00:00:00
abstract::Toll-like receptor (TLR)-based signaling pathways in the host may be modulated by pathogens during the course of infection. We describe a novel immunomodulatory mechanism in which Aspergillus fumigatus conidia induce attenuation of TLR2- and TLR4-mediated interleukin (IL)-6 and IL-1beta proinflammatory responses in hu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01455-08
更新日期:2009-05-01 00:00:00
abstract::Diverse pathogens have evolved to survive and replicate in the endosomes or phagosomes of the host cells and establish persistent infection. Ehrlichiae are Gram-negative, intracellular bacteria that are transmitted by ticks. Ehrlichiae reside in the endosomes of the host phagocytic or endothelial cells and establish p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02101-14
更新日期:2014-10-01 00:00:00
abstract::To study the specific role of transmembrane tumor necrosis factor (tmTNF) in protective and pathological responses against the gastrointestinal helminth Trichinella spiralis, we compared the immune responses of TNF-alpha/lymphotoxin alpha (LTalpha)(-/-) mice expressing noncleavable transgenic tmTNF to those of TNF-alp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01461-08
更新日期:2009-09-01 00:00:00
abstract::Cell-mediated immune (CMI) responses and tumor necrosis factor alpha (TNF-alpha) have been shown to be essential in acquired protection against Cryptococcus neoformans. Induction of a protective anticryptococcal CMI response includes increases in dendritic cells (DC) and activated CD4(+) T cells in draining lymph node...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.1.68-74.2003
更新日期:2003-01-01 00:00:00
abstract::Chlamydia trachomatis is a strict human pathogen producing infections that cause medically important chronic inflammatory diseases, such as blinding trachoma and tubal factor infertility. Isolates exist as serotypes that fall into distinct biologic and pathological groups corresponding to differences in infection tiss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7063-7072.2004
更新日期:2004-12-01 00:00:00
abstract::Haemophilus ducreyi is resistant to killing by normal serum antibody and complement. We discovered an H. ducreyi outer membrane protein required for expression of serum resistance and termed it DsrA (for "ducreyi serum resistance A"). The dsrA locus was cloned, sequenced, and mutagenized. An isogenic mutant (FX517) of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1608-1619.2000
更新日期:2000-03-01 00:00:00