Abstract:
:The actions of polymyxin B, rabbit polymorphonuclear lysosome extracts, 14 polycationic peptides (including defensin NP-2, cecropin P1, lactoferricin B, and active peptides from cationic protein 18 and bactenecin), EDTA, and Tris on Brucella spp. were studied, with other gram-negative bacteria as controls. Brucella spp. were comparatively resistant to all of the agents listed above and bound less polymyxin B, and their outer membranes (OMs) were neither morphologically altered nor permeabilized to lysozyme by polymyxin B concentrations, although both effects were observed for controls. EDTA and peptides increased or accelerated the partition of the hydrophobic probe N-phenyl-naphthylamine into Escherichia coli and Haemophilus influenzae OMs but had no effect on Brucella OMs. Since Brucella and H. influenzae OMs are permeable to hydrophobic compounds (G. Martínez de Tejada and I. Moriyón, J. Bacteriol. 175:5273-5275, 1993), the results show that such unusual permeability is not necessarily related to resistance to polycations. Although rough (R) B. abortus and B. ovis were more resistant than the controls were, there were qualitative and quantitative differences with smooth (S) brucellae; this may explain known host range and virulence differences. Brucella S-lipopolysaccharides (LPSs) had reduced affinities for polycations, and insertion of Brucella and Salmonella montevideo S-LPSs into the OM of a Brucella R-LPS mutant increased and decreased, respectively, its resistance to cationic peptides. The results show that the core lipid A of Brucella LPS plays a major role in polycation resistance and that O-chain density also contributes significantly. It is proposed that the features described above contribute to Brucella resistance to the oxygen-independent systems of phagocytes.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Martínez de Tejada G,Pizarro-Cerdá J,Moreno E,Moriyón Idoi
10.1128/IAI.63.8.3054-3061.1995subject
Has Abstractpub_date
1995-08-01 00:00:00pages
3054-61issue
8eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::Small-colony variants (SCVs) are slow-growing variants of human bacterial pathogens. They are associated with chronic persistent infections, and their biochemical identification and antimicrobial treatment are impaired by altered metabolic properties. To contribute to the understanding of SCV-mediated infections, we a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2254-2262.2004
更新日期:2004-04-01 00:00:00
abstract::Raising the magnesium level from 0.4 to 1.5 mM in a medium containing only amino acids (2.08%), salts, and vitamins increased enterotoxin B production by Staphylococcus aureus S-6 by about 80%. The level of phosphates in the medium was lowered to one-tenth (to 2.87 mM) the original amount without adversely affecting g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.158-160.1978
更新日期:1978-04-01 00:00:00
abstract::We examined the way the major outer membrane protein OmpA of Salmonella enterica serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs) against this protein. Interaction between OmpA and these MAbs is competitively inhibited with several-hundredfold dilution...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3937-3946.2003
更新日期:2003-07-01 00:00:00
abstract::Five monoclonal antibodies (MAbs) were partially characterized and tested for their ability to inhibit penetration of Madin-Darby bovine kidney (MDBK) cells by sporozoites of Eimeria bovis. By indirect fluorescent-antibody assays, all MAbs reacted with acetone-fixed sporozoites, but only two MAbs, EbS9 (immunoglobulin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2538-2543.1988
更新日期:1988-10-01 00:00:00
abstract::Human multidrug efflux transporters are known for their ability to extrude antibiotics and toxic compounds out of cells, yet accumulating data indicate they have additional functions in diverse physiological processes not related to drug efflux. Here, we show that the human multidrug transporter P-glycoprotein (P-gp) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00380-15
更新日期:2015-06-01 00:00:00
abstract::Levels of the serum opsonin mannan-binding lectin (MBL) were directly correlated with the probability of developing visceral leishmaniasis. Monocytes infected with MBL-opsonized Leishmania chagasi promastigotes secreted higher levels of tumor necrosis factor alpha and interleukin-6 than cells infected with nonopsonize...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5212-5215.2001
更新日期:2001-08-01 00:00:00
abstract::Athymic BALB/c nude mice and euthymic BALB/c mice were infected with Rickettsia akari by the intraperitoneal route. The rickettsialpox infection was terminated in euthymic mice with only two intraperitoneal injections of the antibiotic oxytetracycline, whereas prolonged treatment was necessary to terminate the infecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Mice injected intravenously with a commercially available extract of Coxiella burnetii prepared for use as the antigen in the complement fixation diagnostic test for Q fever were subsequently resistant to infection with Babesia microti, Babesia rodhaini, and Plasmodium vinckei petteri. The parasites appeared to die in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.319-325.1979
更新日期:1979-05-01 00:00:00
abstract::In the mouse model of Lyme borreliosis, the host immune response during infection with Borrelia burgdorferi results in the remission of carditis and arthritis, as well as global reduction of spirochete numbers in tissues, without elimination of infection. These events were recapitulated by passive transfer of immune s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00469-06
更新日期:2006-08-01 00:00:00
abstract::In this study, the role of the hepatobiliary system in the early pathogenesis of Salmonella enteritidis infection was investigated in a rat model. Intravenous (i.v.) challenge with lipopolysaccharide (LPS) has previously been shown to enhance the translocation of normal gut flora. We first confirmed that LPS can simil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.1-5.2000
更新日期:2000-01-01 00:00:00
abstract::Although recent evidence suggests that fibronectin may be involved in the attachment of treponemes to mammalian cells, its possible role in promoting phagocytosis of Treponema pallidum has not been investigated. In the present study, we examined the antibody-independent interactions of fibronectin, C1q, and human poly...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.456-464.1986
更新日期:1986-11-01 00:00:00
abstract::Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1529-1534.1995
更新日期:1995-04-01 00:00:00
abstract::The binding of (125)I-labeled S component to rabbit polymorphonuclear leukocytes was found to be concentration dependent and saturable at 37 degrees C. Scatchard analysis of the binding curve gave a straight line, indicating that S component binds to a single population of sites. The dissociation constant, K(D), deriv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.362-367.1981
更新日期:1981-11-01 00:00:00
abstract::Lipid compositions of 40 patient isolates of Mycobacterium tuberculosis derived from Madras, Burma, Rangoon, and East Africa were studied, and two major populations of tubercle bacilli were distinguished. Nearly all of the strains previously designated as attenuated for the guinea pig (D. A. Mitchison) are characteriz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.150-158.1974
更新日期:1974-01-01 00:00:00
abstract::A main feature in the pathogenesis of bacterial endocarditis is the activation of the coagulation system via the extrinsic pathway, resulting in the formation of infected endocardial vegetations. Earlier studies gave indirect evidence that monocytes play an important role in the procoagulant response during the course...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.448-451.1996
更新日期:1996-02-01 00:00:00
abstract::Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). A murine UTI model has revealed an infection cascade whereby UPEC undergoes cycles of invasion of the bladder epithelium, intracellular proliferation in polysaccharide-containing biofilm-like masses called intracellular bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00925-09
更新日期:2010-03-01 00:00:00
abstract::Protective immunity in mice to the infective third-stage larvae (L3) of Strongyloides stercoralis was shown to be dependent on immunoglobulin M (IgM), complement activation, and granulocytes. The objectives of the present study were to determine whether IgG was also a protective antibody isotype and to define the spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.6835-6843.2003
更新日期:2003-12-01 00:00:00
abstract::Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.170-177.1983
更新日期:1983-10-01 00:00:00
abstract::In a previous study we showed that the involvement of EP4 subtype of the prostaglandin E (PGE) receptor is crucial for lipopolysaccharide (LPS)-induced osteoclast formation in vitro. The present study was undertaken to test whether EP4 is actually associated with LPS-induced bone resorption in vivo. In wild-type (WT) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6819-6825.2000
更新日期:2000-12-01 00:00:00
abstract::Peptidoglycans of Leptotrichia buccalis ATCC 14201 and ATCC 19616 were isolated by extraction with sodium dodecyl sulfate and subsequent digestion of the sodium dodecyl sulfate-insoluble residue with proteases and alpha-amylase. Cell wall fractions obtained by sodium dodecyl sulfate extraction and protease digestion w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.132-136.1983
更新日期:1983-01-01 00:00:00
abstract::The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1424-1431.1999
更新日期:1999-03-01 00:00:00
abstract::The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.751-755.1977
更新日期:1977-03-01 00:00:00
abstract::Mouse fibroblasts (L cells) infected with the 6BC strain of Chlamydia psittaci released potassium ion (K(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. When the multiplicity of infection was 500 to 1,000 50% infectious units (ID(50)) per L cell, loss of intracell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.827-832.1978
更新日期:1978-03-01 00:00:00
abstract::Antigenic competition between bovine gamma globulin (BGG) and endotoxin from a smooth strain (S-ET) and a rough (R-ET) heptoseless mutant strain of Salmonella minnesota was studied in mice. Both endotoxins acted as adjuvants for enhancing the antibody response to BGG. However, other work showed that the R-ET had minim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.2.308-317.1971
更新日期:1971-02-01 00:00:00
abstract::Metastatic infection after hematogenous dissemination of Candida species is presumably dependent on the fungus traversing the vascular endothelium. An in vitro model of the earliest events of metastatic Candida infection was developed with whole vascular strips. Freshly obtained porcine blood vessels were secured in a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.374-384.1983
更新日期:1983-10-01 00:00:00
abstract::Eight chlamydial isolates of ovine origin were tested in a plaque reduction system using homologous and heterologous rooster antisera. The eight isolates could be separated into two separate immunotypes. Type 1 included isolates associated with ovine abortion and one agent recovered from the feces of an apparently nor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.92-94.1974
更新日期:1974-01-01 00:00:00
abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.1106-1108.1976
更新日期:1976-10-01 00:00:00
abstract::The ocular surface is continuously exposed to potential pathogens, including free-living amoebae. Acanthamoeba species are among the most ubiquitous amoebae, yet Acanthamoeba keratitis is remarkably rare. The pathogenesis of Acanthamoeba keratitis is a complex, sequential process. Here we show that Acanthamoeba kerati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.1061-1068.2005
更新日期:2005-02-01 00:00:00
abstract::Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2285-2288.1994
更新日期:1994-06-01 00:00:00
abstract::Conditions for the release of streptococcal type 12 M protein from whole cells by cyanogen bromide are described; they demonstrated that methionine is not essential to the structural arrangements which account for some of its immunological and biological properties. The released M protein was separated from other prot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.546-555.1978
更新日期:1978-08-01 00:00:00