Abstract:
:We have previously shown that infection by Trypanosoma cruzi, a parasitic protozoan, is reduced by injection of CD40 ligand (CD40L)-transfected 3T3 fibroblasts (D. Chaussabel, F. Jacobs, J. de Jonge, M. de Veerman, Y. Carlier, K. Thielemans, M. Goldman, and B. Vray, Infect. Immun. 67:1929-1934, 1999). This prompted us to transfect T. cruzi with the murine CD40L gene and to study the consequences of this transfection on the course of infection. For this, epimastigotes (Y strain) were electroporated with the pTEX vector alone or the pTEX-CD40L construct, and transfected cells were selected for their resistance to Geneticin G418. Then strain Y-, pTEX-, and pTEX-CD40L-transfected epimastigotes were transformed by metacyclogenesis into mammalian infective forms called Y, YpTEX, and YpTEX-CD40L trypomastigotes. Transfection of the CD40L gene and expression of the CD40L protein were assessed by reverse transcription-PCR and Western blot analysis. The three strains of parasites were infective in vitro for mouse peritoneal macrophages. When organisms were inoculated into mice, a very low level of parasitemia and no mortality were seen with the YpTEX-CD40L strain compared to the Y and YpTEX strains. Furthermore, the proliferative capacity and the secretion of gamma interferon were both preserved in spleen cells (SCs) from YpTEX-CD40L-infected mice but not with SCs from Y- and YpTEX-infected mice. These results suggest that the CD40L produced by transfected T. cruzi is involved in the modulation of an antiparasite immune response. Moreover, mice surviving YpTEX-CD40L infection resisted a challenge infection with the wild-type strain. Taken together, our data demonstrate the feasibility of generating a T. cruzi strain expressing a bioactive host costimulatory molecule that counteracts the immunodeficiency induced by the parasite during infection and enhances protective immunity against a challenge infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Chamekh M,Vercruysse V,Habib M,Lorent M,Goldman M,Allaoui A,Vray Bdoi
10.1128/IAI.73.10.6552-6561.2005keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
6552-61issue
10eissn
0019-9567issn
1098-5522pii
73/10/6552journal_volume
73pub_type
杂志文章abstract::An extracellular cytolysin produced by Vibrio metschnikovii was purified by acid precipitation, phenyl-Sepharose CL-4B chromatography, and rechromatography on a phenyl-Sepharose CL-4B column and high-performance liquid chromatography on a Mono Q (anion-exchange) column. The purified cytolysin had a molecular weight of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.954-960.1988
更新日期:1988-04-01 00:00:00
abstract::Pneumolysin is an important virulence factor of Streptococcus pneumoniae, interacting with the membranes of host cells to elicit a multitude of inflammatory responses. We used cDNA microarrays to identify genes which are responsive to S. pneumoniae in a pneumolysin-dependent and -independent fashion. The THP-1 human m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2087-2094.2003
更新日期:2003-04-01 00:00:00
abstract::We have cloned and sequenced the d15 gene from two strains of Haemophilus influenzae type b (Hib) and two strains of nontypeable H. influenzae (NTHI). The nucleotide and deduced protein sequences of d15 are highly conserved, with only a small variable region identified near the carboxyl terminus of the protein. Analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3701-3707.1997
更新日期:1997-09-01 00:00:00
abstract::B-cell-deficient C57B1/6J mice (suppressed from birth with goat anti-mu) and controls (treated from birth with normal goat serum) were infected with Trypanosoma rhodesiense. There was a significant (P less than 0.01) decrease in duration of survival of the mu-suppressed mice compared with that of controls. Whereas bot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.434-438.1977
更新日期:1977-11-01 00:00:00
abstract::Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella abortus (BCSP31). Recombinant S. choleraesuis chi 3781 stably maintained the pBA31-R7 plasmid and continued to express t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.610-618.1993
更新日期:1993-02-01 00:00:00
abstract::Iron is limiting in the human host, and bacterial pathogens respond to this environment by regulating gene expression through the ferric uptake regulator protein (Fur). In vitro studies have demonstrated that Neisseria gonorrhoeae controls the expression of several critical genes through an iron- and Fur-mediated mech...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4281-4287.2005
更新日期:2005-07-01 00:00:00
abstract::Although the periodontal pathogen Porphyromonas gingivalis must withstand high levels of nitrosative stress while in the oral cavity, the mechanisms of nitrosative stress defense are not well understood in this organism. Previously we showed that the transcriptional regulator HcpR plays a significant role in defense, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00572-18
更新日期:2019-03-25 00:00:00
abstract::Macrophage-dependent killing of facultative intracellular bacteria was markedly impaired by overt erythrocytic Plasmodium yoelii or Plasmodium berghei infection of mice. P. yoelii infection was capable of ablating not only the macrophage microbicidal capacity of "normal" animals but also the bactericidal capacities of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.396-407.1981
更新日期:1981-01-01 00:00:00
abstract::Although excystation is crucial to the initiation of infection by Giardia lamblia, little is known about the regulation of this important process. We have been able to reliably induce excystation in vitro by mimicking cyst passage through the stomach and upper small intestine by the exposure of in vitro-derived cysts ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2151-2157.1996
更新日期:1996-06-01 00:00:00
abstract::The intraerythrocytic apicomplexan Babesia microti, the primary causative agent of human babesiosis, is a major public health concern in the United States and elsewhere. Apicomplexans utilize a multiprotein complex that includes a type I membrane protein called apical membrane antigen 1 (AMA1) to invade host cells. We...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00168-15
更新日期:2015-10-01 00:00:00
abstract::A set of strain- and size-variant highly immunogenic membrane surface protein antigens of Mycoplasma bovis, which has been identified by a monoclonal antibody, is shown in this report to make up a family of antigenically and structurally related lipid-modified proteins, designated Vsps (variable surface proteins). By ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.5075-5084.1994
更新日期:1994-11-01 00:00:00
abstract::The character of the antibody response in the rabbit to Salmonella typhimurium somatic (O) antigen was similar to the response to each of several serotypes of Shigella flexneri O antigens, namely a predominance of production of immunoglobulin M (IgM) antibody. Lipopolysaccharide protein (LPSP) and lipopolysaccharide (...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1970-03-01 00:00:00
abstract::Mice orally immunized with Salmonella dublin EL23, a nonreverting, aromatic-dependent, histidine-requiring mutant transformed with a plasmid which carries a gene that codes for production of the B subunit of the heat-labile toxin (LT-B) of enterotoxigenic Escherichia coli, or with purified LT-B alone were compared for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.1076-1083.1995
更新日期:1995-03-01 00:00:00
abstract::Colony counts and light microscopy were used to assess the capacity of Candida albicans to colonize, infect the alimentary tract, and cause disseminated disease in athymic (nu/nu), euthymic (nu/+), beige (bg/bg), black (bg/+), beige athymic (bg/bg nu/nu), or beige euthymic (bg/bg nu/+) germfree mice. The alimentary tr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1093-1100.1990
更新日期:1990-04-01 00:00:00
abstract::Haemophilus ducreyi, the causative agent of chancroid, produces a lipooligosaccharide (LOS) which terminates in N-acetyllactosamine. This glycoform can be further extended by the addition of a single sialic acid residue to the terminal galactose moiety. H. ducreyi does not synthesize sialic acid, which must be acquire...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6727-6735.2005
更新日期:2005-10-01 00:00:00
abstract::Streptococcus iniae causes meningoencephalitis and death in commercial fish species and has recently been identified as an emerging human pathogen producing fulminant soft tissue infection. As identified by pulsed-field gel electrophoresis (PFGE), strains causing disease in either fish or humans belong to a single clo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.1994-2000.2001
更新日期:2001-04-01 00:00:00
abstract::Comparisons of the course of coccidioidomycosis in two strains of inbred mice established that BALB/c mice are significantly more susceptible to pulmonary infection with Coccidioides immitis than are DBA/2 mice. The susceptibility of BALB/c mice does not reside in their inability to mount a delayed-type hypersensitivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.13-17.1988
更新日期:1988-01-01 00:00:00
abstract::Science has always been a competitive undertaking. Despite recognition of the benefits of cooperation and team science, reduced availability of funding and jobs has made science more competitive than ever. Here we consider the benefits of competition in providing incentives to scientists and the adverse effects of com...
journal_title:Infection and immunity
pub_type: 社论
doi:10.1128/IAI.02939-14
更新日期:2015-04-01 00:00:00
abstract::Previous studies have shown that tumor necrosis factor alpha (TNF-alpha) plays a pathophysiologic role in sepsis induced in rat pups by group B streptococci (GBS). In this model, TNF-alpha is also partially responsible for the induction of interleukin-6 (IL-6). The present study was undertaken to investigate the role ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4997-5002.1994
更新日期:1994-11-01 00:00:00
abstract::Vibrio vulnificus causes rapidly progressing septicemia with an extremely high mortality rate (≥50%), even with aggressive antibiotic treatment. The bacteria secrete multifunctional autoprocessing repeats-in-toxin (MARTX) toxins, which are involved in the pathogenesis of Gram-negative Vibrio species. Recently, we repo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02130-14
更新日期:2014-11-01 00:00:00
abstract::Mice immunized intravenously 10 days earlier (but not those immunized 2 months earlier) with an attenuated Salmonella typhimurium SL3261 aroA live vaccine and tested for delayed-type hypersensitivity by injection of crude Salmonella extracts in the footpad can die within 24 to 48 h of an unexplained allergic reaction....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.6.2285-2288.1994
更新日期:1994-06-01 00:00:00
abstract::The serum antibody response to proteins encoded by the virulence-associated plasmid of Shigella flexneri was determined in monkeys challenged with virulent S. flexneri serotype 2a. With water-extractable antigen in an enzyme-linked immunosorbent assay, a significant increase in antibody titer against proteins from a p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.57-63.1986
更新日期:1986-07-01 00:00:00
abstract::Obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. Anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01418-09
更新日期:2010-05-01 00:00:00
abstract::In the present study, the roles of interleukin 12 (IL-12) and IL-18 and their possible interaction during superantigen-induced responses were studied by injection of staphylococcal enterotoxin B (SEB) into mice. These data suggest that the role of IL-12 in SEB-induced responses is limited to sustaining gamma interfero...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5949-5952.2001
更新日期:2001-09-01 00:00:00
abstract::Antimicrobial peptides, such as defensins or cathelicidins, are effector substances of the innate immune system and are thought to have antimicrobial properties that contribute to host defense. The evidence that vertebrate antimicrobial peptides contribute to innate immunity in vivo is based on their expression patter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6084-6089.1999
更新日期:1999-11-01 00:00:00
abstract::Encapsulated Streptococcus pneumoniae of serotypes 2, 9N, 14, 21, and 23F and an unencapsulated variant of type 2 pneumococci were efficiently phagocytosed by both aerobically and anaerobically incubated human leukocytes. In the presence of O2, the pneumococci rapidly lost their viability, whereas during anaerobiosis,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.277-281.1985
更新日期:1985-01-01 00:00:00
abstract::We identified nonhemolytic mutants of Listeria monocytogenes that were severely deficient in their ability to invade mammalian nonprofessional phagocytes. These mutants were generated spontaneously or by means of transposon Tn916 mutagenesis. In terms of their extracellular proteins, the noninvasive mutants were defic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.3988-3995.1990
更新日期:1990-12-01 00:00:00
abstract::The effects of passive administration of neutralizing monoclonal antibodies (MAB) to respiratory syncytial virus glycoproteins (GP90 and VP70) was evaluated in cotton rats challenged with respiratory syncytial virus. Animals injected with MAB to VP70 had lower mean viral titers in lung tissues than did controls (log10...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.756-758.1984
更新日期:1984-02-01 00:00:00
abstract::Mice were inoculated with Legionella pneumophila via an intratracheal route to establish an experimental model of infection. Lung lavage fluid obtained from infected mice contained a cytolytic factor identified as tumor necrosis factor (TNF). Peak levels of TNF were produced at about 24 h postinfection and rapidly dec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.433-437.1987
更新日期:1987-02-01 00:00:00
abstract::Pathogens produce virulence factors that interact directly with host molecules, but in many cases the host targets are unknown. The genetic and molecular identification of these orphan targets is often not feasible with mammalian experimental models. However, a substantial number of known targets are molecules and pat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6271-6275.2001
更新日期:2001-10-01 00:00:00