Abstract:
:Fonsecaea pedrosoi is a fungal pathogen that produces melanin. The functions of melanin and its possible influence in the protective immunological response during infection by F. pedrosoi are not known. In this work, treatment of F. pedrosoi mycelia with proteases and glycosidases followed by a denaturing agent and hot concentrated acid left a black residue. Scanning electron microscopy demonstrated that this processed melanized residue resembled very closely the intact mycelium in shape and size. Melanin particles were also isolated from culture fluids of conidia or sclerotic forms of F. pedrosoi. Secreted melanins were reactive with sera from infected human patients, suggesting that F. pedrosoi synthesizes melanin in vivo. The antibodies against melanin were purified from patients' sera and analyzed by indirect immunofluorescence. They reacted with sclerotic cells from patients' lesions as well as with sclerotic bodies cultivated in vitro, conidia, mycelia, and digested residues. Treatment of F. pedrosoi with purified antibodies against melanin inhibited fungal growth in vitro. The interaction of F. pedrosoi with phagocytes in the presence of melanin resulted in higher levels of fungal internalization and destruction by host cells, which was accompanied by greater degrees of oxidative burst. Taken together, these results indicate that melanin from F. pedrosoi is an immunologically active fungal structure that activates humoral and cellular responses that could help the control of chromoblastomycosis by host defenses.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Alviano DS,Franzen AJ,Travassos LR,Holandino C,Rozental S,Ejzemberg R,Alviano CS,Rodrigues MLdoi
10.1128/iai.72.1.229-237.2004keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
229-37issue
1eissn
0019-9567issn
1098-5522journal_volume
72pub_type
杂志文章abstract::We have previously reported that a wild strain of Francisella tularensis is much less efficiently killed by human polymorphonuclear leukocytes than is an attenuated strain. In the present study, the killing of the attenuated strain was found to be strictly oxygen dependent. The wild and the attenuated strains both ind...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.730-734.1984
更新日期:1984-02-01 00:00:00
abstract::We previously showed the requirement of both T cells and gamma interferon (IFN-gamma)-producing non-T cells for the genetic resistance of BALB/c mice to the development of toxoplasmic encephalitis (TE). In order to define the role of IFN-gamma production and the perforin-mediated cytotoxicity of T cells in this resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4432-4438.2004
更新日期:2004-08-01 00:00:00
abstract::Inflammatory mediators orchestrate the host immune and metabolic response to acute bacterial infections and mediate the events leading to septic shock. Tumor necrosis factor (TNF) has long been identified as one of the proximal mediators of endotoxin action. Recent studies have implicated peroxisome proliferator-activ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3488-3493.1999
更新日期:1999-07-01 00:00:00
abstract::The endothelium is a specific target for Bartonella henselae, and endothelial cell infection represents an important step in the pathogenesis of cat scratch disease and bacillary angiomatosis. Mechanisms of Bartonella-endothelial cell interaction as well as signaling pathways involved in target cell activation were an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5088-5097.2001
更新日期:2001-08-01 00:00:00
abstract::The primary antibody response of BALB/c splenocytes to sheep erythrocytes in vitro was suppressed by infection with Friend leukemia virus (FLV), with the response capacity decreasing with increasing duration of infection. The acquisition of normal antibody responses was amplified by macrophage-produced antibody respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1260-1264.1983
更新日期:1983-03-01 00:00:00
abstract::In previous work (Jones, Infect, Immun. 30:78-89, 1980) a major cytoplasmic antigen of Candida albicans was identified. In both humans and experimental animals, this antigen is released from C. albicans during the course of an invasive C. albicans infection and elicits a specific antibody response. In this study, we u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.469-477.1981
更新日期:1981-11-01 00:00:00
abstract::The influence of bile acids on adenosine 3',5'-phosphate-induced intestinal secretion was studied in mice. Bile flow was stopped by ligation of the common bile duct, and secretion was induced in ligated loops of the small intestine. The decrease of bile led to inhibition of hypersecretion after challenge with heat-lab...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.391-395.1982
更新日期:1982-02-01 00:00:00
abstract::Purification of the envelope antigen of Yersinia pestis EV with passive hemagglutination activity is described. The purification procedure consisted of pancreatin digestion, chromatography on human erythrocyte stroma set on Celite, and rechromatography on Sephadex G-200. Chemical, physical, and biological properties o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.798-803.1978
更新日期:1978-12-01 00:00:00
abstract::Toxin preparations were obtained by growing Clostridium difficile VPI strain 10463 in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM-100 membrane filter. Two toxic activities, designated toxins A and B, were se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.1032-1040.1982
更新日期:1982-03-01 00:00:00
abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2182-2191.2003
更新日期:2003-04-01 00:00:00
abstract::Comparative studies on the virulence of two strains of Pseudomonas aeruginosa isolated from otic pus and mink pneumonic lesion, respectively, were made by means of intranasal inoculation in mink. The minimal lethal dose of the former (strain NC-5, highly virulent for mice) was 10(10), and that of the latter (strain No...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.16-20.1974
更新日期:1974-07-01 00:00:00
abstract::Nonviable Mycobacterium tuberculosis strain Jamaica suspended in oil-droplet emulsions was used to enhance resistance of mice against encephalomyocarditis virus (EMCV). The mycobacteria-injected mice were significantly resistant to 50,000 50% lethal doses of EMCV. Similar concentrations of virus in plasma of normal an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.740-745.1978
更新日期:1978-12-01 00:00:00
abstract::The effect of human-specific antibody on surface membrane antigens of trypomastigotes of Trypanosoma cruzi was studied in vitro by using immunofluorescence methods. Immune sera induced aggregation of surface antigens in trypomastigotes to form polar cell caps. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.567-569.1978
更新日期:1978-05-01 00:00:00
abstract::Survival of macrophage microbicidal activity is a prerequisite for invasive disease caused by the enteric pathogen Salmonella enterica serovar Typhimurium. Flavohemoglobins, such as those of Escherichia coli, Salmonella, and yeast, play vital roles in protection of these microorganisms in vitro from nitric oxide (NO) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4399-4405.2002
更新日期:2002-08-01 00:00:00
abstract::Bordetella bronchiseptica is a Gram-negative bacterium that infects and causes disease in a wide variety of animals. B. bronchiseptica also infects humans, thereby demonstrating zoonotic transmission. An extensive characterization of human B. bronchiseptica isolates is needed to better understand the distinct genetic ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01453-13
更新日期:2014-04-01 00:00:00
abstract::Inflammatory cytokines play an important role in human immune responses to malarial disease. However, the role of these mediators in disease pathogenesis, and the relationship between host protection and injury remains unclear. A total of 248 cases of severe Plasmodium falciparum malaria among children aged 3 months t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5630-5637.2004
更新日期:2004-10-01 00:00:00
abstract::Five monoclonal antibodies (MAbs) were partially characterized and tested for their ability to inhibit penetration of Madin-Darby bovine kidney (MDBK) cells by sporozoites of Eimeria bovis. By indirect fluorescent-antibody assays, all MAbs reacted with acetone-fixed sporozoites, but only two MAbs, EbS9 (immunoglobulin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2538-2543.1988
更新日期:1988-10-01 00:00:00
abstract::Chromosomal DNA from a uropathogenic strain of Escherichia coli was partially digested with the restriction enzyme EcoRI. The partial digests were ligated into a cosmid containing an ampicillin-resistant determinant and packaged into lambda phage particles. An ampicillin-resistant transductant of E. coli HB101 was fou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.739-744.1982
更新日期:1982-11-01 00:00:00
abstract::Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
abstract::The extracellular cysteine protease from Streptococcus pyogenes is a virulence factor that plays a significant role in host-pathogen interaction. Streptococcal protease is expressed as an inactive 40-kDa precursor that is autocatalytically converted into a 28-kDa mature (active) enzyme. Replacement of the single cyste...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4326-4333.1999
更新日期:1999-09-01 00:00:00
abstract::Yersinia pestis causes a rapid, lethal disease referred to as plague. Y. pestis actively inhibits the innate immune system to generate a noninflammatory environment during early stages of infection to promote colonization. The ability of Y. pestis to create this early noninflammatory environment is in part due to the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00909-19
更新日期:2020-02-20 00:00:00
abstract::In this study, a novel recombinant attenuated Yersinia pseudotuberculosis PB1+ strain (χ10069) engineered with ΔyopK ΔyopJ Δasd triple mutations was used to deliver a Y. pestis fusion protein, YopE amino acid 1 to 138-LcrV (YopENt138-LcrV), to Swiss Webster mice as a protective antigen against infections by yersiniae....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00296-19
更新日期:2019-09-19 00:00:00
abstract::Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env) proteins that were not found on vibrios after in vitro culture in various ordinary liquid media. At the same time, several of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1809-1815.1989
更新日期:1989-06-01 00:00:00
abstract::Streptococcus iniae causes meningoencephalitis and death in commercial fish species and has recently been identified as an emerging human pathogen producing fulminant soft tissue infection. As identified by pulsed-field gel electrophoresis (PFGE), strains causing disease in either fish or humans belong to a single clo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.1994-2000.2001
更新日期:2001-04-01 00:00:00
abstract::Sterile sonic-treated materials of pneumococci grown in media containing N-acetylmannosamine as the carbohydrate source were lethal for mice. Purification of the toxic factor led to its identification as the enzyme neuraminidase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.115-117.1970
更新日期:1970-07-01 00:00:00
abstract::Mast cell-deficient W/Wv mice and their mast cell-sufficient littermates were given infections of Trichinella spiralis. W/Wv mice were slower than their littermates to expel adult T. spiralis. Repair of the mast cell deficiency of W/Wv mice by bone marrow grafting was accompanied by accelerated expulsion of T. spirali...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.445-447.1983
更新日期:1983-07-01 00:00:00
abstract::Naturally occurring antibodies for Vibrio fetus with whole-cell and O antigen specificities were detected in sera of mature cattle by means of the agglutination and indirect immunofluorescence reactions. O antibodies occurred in each of 21 sera examined, and whole-cell antibodies, in lower concentrations, occurred in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.728-733.1972
更新日期:1972-05-01 00:00:00
abstract::An immunosuppressed rat model of Pneumocystis carinii pneumonia is described that utilizes simple, noninvasive intratracheal (i.t.) inoculation of cryopreserved parasites and results in development of severe P. carinii pneumonia within 5 weeks. This is an improvement over the most commonly used models of P. carinii pn...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1589-1597.1992
更新日期:1992-04-01 00:00:00
abstract::The precise role of Leishmania glycoconjugate molecules including phosphoglycans (PGs) and lipophosphoglycan (LPG) on host cellular responses is still poorly defined. Here, we investigated the interaction of Leishmania major LPG2 null mutant (lpg2(-)), which lacks both PGs and LPG, with dendritic cells (DCs) and the s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01447-08
更新日期:2009-08-01 00:00:00