Abstract:
:In mice infected with virulent blood (trypomastigote) forms of Trypanosoma cruzi, complement depletion with cobra venom factor caused a marked exacerbation of the disease evidenced by significantly increased parasitemia levels and early mortality as compared with those of untreated infected animals. The effect was greater in mice receiving cobra venom factor on day 7 postinfection, i.e., at the time when the parasites had had time to localize and multiply in the tissues and appeared in the circulation in appreciable numbers. The possibility that complement participates in host defense against T. cruzi infection through a mechanism involving immune lysis was explored in vitro. T. cruzi trypomastigotes were found to undergo immune lysis in sera of patients with chronic Chagas' disease, in sera of immunized mice, and in solutions containing both immune mouse gamma globulin and a source of active complement. This phenomenon failed to take place either in the absence of complement or after complement inactivation by heat or utilizing complement inactivators. The lytic capacity of heated sera was restored by the addition of active complement to the system. During the immune lysis of T. cruzi blood forms, complement was activated in human sera via both the classical and the alternate pathways. In mouse sera, activation followed at least the alternate pathway.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Budzko DB,Pizzimenti MC,Kierszenbaum Fdoi
10.1128/IAI.11.1.86-91.1975subject
Has Abstractpub_date
1975-01-01 00:00:00pages
86-91issue
1eissn
0019-9567issn
1098-5522journal_volume
11pub_type
杂志文章abstract::C4BP (C4b-binding protein) is a high-molecular-weight plasma protein that inhibits the classical pathway of complement activation. Recent experiments have demonstrated that C4BP binds to many strains of the gram-positive bacterium Streptococcus pyogenes, a major respiratory tract pathogen. Binding to S. pyogenes was s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3638-3643.1997
更新日期:1997-09-01 00:00:00
abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.670-674.1983
更新日期:1983-05-01 00:00:00
abstract::Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05851-11
更新日期:2012-02-01 00:00:00
abstract::Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1391-1396.1974
更新日期:1974-12-01 00:00:00
abstract::In order to further understand Epstein-Barr virus (EBV)-lymphocyte interactions, we investigated a chain of events including: (i) EBV binding to human lymphocyte subpopulations; (ii) the earliest appearance of EBV-determined nuclear antigen (EBNA) in the lymphocytes after EBV infection; and (iii) establishment of cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.2.303-310.1976
更新日期:1976-02-01 00:00:00
abstract::A dialyzable factor(s) in human serum is known to stimulate gonococcal oxygen consumption. Its effect on other human pathogens was investigated. A 10% serum solution increased peak O2 consumption for Escherichia coli and Staphylococcus aureus to 157% (P less than 0.05) and 199% (P less than 0.02), respectively, of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.657-663.1986
更新日期:1986-06-01 00:00:00
abstract::Blastomyces dermatitidis is a thermally induced dimorphic fungus capable of causing lung and systemic infections in immunocompetent animal hosts. With the publication of genomic sequences from three different strains of B. dermatitidis and the development of RNA interference as a gene-silencing tool, it has become pos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01328-09
更新日期:2010-06-01 00:00:00
abstract::A nonclonal population of Borrelia burgdorferi N40 (passage 3) that survived protective immunity following challenge inoculation of outer surface protein (Osp) A- or B-hyperimmunized mice were characterized for the molecular basis of evasion of immunity. Two of six B. burgdorferi isolates, cultured from OspA-immunized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1658-1662.1995
更新日期:1995-05-01 00:00:00
abstract::Neisseria gonorrhoeae, the etiologic agent of gonorrhea, is frequently asymptomatic in women, often leading to chronic infections. One factor contributing to this may be biofilm formation. N. gonorrhoeae can form biofilms on glass and plastic surfaces. There is also evidence that biofilm formation may occur during nat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00036-09
更新日期:2009-09-01 00:00:00
abstract::Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.511-517.1984
更新日期:1984-08-01 00:00:00
abstract::Vero cytotoxin-producing Escherichia coli (VTEC) strains have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. Since adherence of enteric pathogens to epithelial surfaces is often a prerequisite for the subsequent delivery of bacterial enterotox...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.756-761.1988
更新日期:1988-04-01 00:00:00
abstract::Pseudomonas aeruginosa utilizes a number of distinct pathways to secrete proteins that play various roles during infection. These include the type II secretion system, which is responsible for the secretion of the majority of exoproducts into the surrounding environment, including toxins and degradative enzymes. In co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1695-1705.2005
更新日期:2005-03-01 00:00:00
abstract::Previous studies have reported that human vascular endothelial cells lack the membrane-bound lipopolysaccharide (LPS) receptor, CD14 (mCD14). By optimizing assay conditions, including the selection of anti-CD14 monoclonal antibody, we now demonstrate that human umbilical vein endothelial cells (HUVEC) express CD14 on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.479-485.2001
更新日期:2001-01-01 00:00:00
abstract::Two glucosyltransferase genes from Streptococcus mutans GS-5, gtfB and gtfC, have been previously isolated and sequenced in this laboratory. In the present communication a third gtf gene, gtfD, was isolated and characterized. Isolation of the gene involved a novel procedure utilizing the integration plasmid pVA891. A ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.2079-2085.1989
更新日期:1989-07-01 00:00:00
abstract::Transplacental infection of mouse fetuses with Venezuelan equine encephalomyelitis was produced by intraperitoneal injection of dams at various stages of gestation with 10(3) suckling mouse intracerebral median lethal doses of an attenuated strain (TC-83). Virus inoculation, at times ranging from 6 days prior to matin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.339-343.1972
更新日期:1972-09-01 00:00:00
abstract::Complement is a critical component of antimicrobial immunity. Various complement regulatory proteins prevent host cells from being attacked. Many pathogens have acquired the ability to sequester complement regulators from host plasma to evade complement attack. We describe here how Streptococcus pneumoniae adopts a st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00742-18
更新日期:2018-12-19 00:00:00
abstract::Encapsulated Staphylococcus aureus strains are more virulent than unencapsulated staphylococci, and this phenomenon has been associated with decreased opsonization of encapsulated bacteria by normal human serum. Peptidoglycan, a major cell wall component of S. aureus, has been shown to promote opsonization of this bac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1159-1163.1979
更新日期:1979-12-01 00:00:00
abstract::The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a signi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7170-7172.2003
更新日期:2003-12-01 00:00:00
abstract::A 95-kDa protein was isolated from Staphylococcus saprophyticus 7108 grown on dialysis membranes placed on the surface of brain heart infusion agar. Strain CCM883 did not produce this protein. Ultrathin sections revealed the presence of very thin, tuftlike, 50- to 75-nm-long structures on the surface of strain 7108, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.1055-1060.1992
更新日期:1992-03-01 00:00:00
abstract::Mycoplasma gallisepticum is an avian respiratory and reproductive tract pathogen that has a significant economic impact on the poultry industry worldwide. Although membrane proteins of Mycoplasma spp. are thought to play crucial roles in host interactions, very few have had their biochemical function defined. In this ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-19
更新日期:2019-08-21 00:00:00
abstract::Of 51 patients with herpes simplex labialis, 50 had detectable interferon (IFN) in samples of lesion vesicle fluid. The median titer of vesicle fluid IFN was 8,200 U. and the range of values was 400 to 63,600 U. The amount of vesicle fluid IFN was correlated with lesion age (r = 0.32, P = 0.024) and vesicle fluid viru...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.3.907-910.1982
更新日期:1982-06-01 00:00:00
abstract::To define the role of nontypeable Haemophilus influenzae (NTHI) lipooligosaccharide (LOS) in the induction of proinflammatory cytokine gene expression during otitis media, we compared the abilities of formalin-killed NTHI strain 2019 and its LOS htrB and rfaD mutants to stimulate human middle ear epithelial (HMEE) cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3678-3684.2001
更新日期:2001-06-01 00:00:00
abstract::Legionella pneumophila is the predominant cause of Legionnaires' disease in the United States and Europe, while Legionella longbeachae is the common cause of the disease in Western Australia. Although clinical manifestations by both intracellular pathogens are very similar, recent studies have shown that phagosome bio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00025-07
更新日期:2007-04-01 00:00:00
abstract::Vitamin E protects nonimmunized and immunized mice against fatal Diplococcus pneumoniae type I (DpI) infection. A dietary supplementation of 180 mg of dl-alpha-tocopheryl acetate per kg of diet increased survival of nonimmunized mice from 20 to 80% when challenged with 20 organisms, and of mice immunized with 0.5 ng o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1292-1295.1974
更新日期:1974-12-01 00:00:00
abstract::Trehalose 6,6'-dimycolate (TDM) plays important roles in the development of granulomatous inflammation during infection with Mycobacterium spp., Rhodococcus spp., etc. To reveal the augmenting effect of TDM on vascular endothelial growth factor (VEGF) production and neovascularization, we investigated murine granuloma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2043-2052.2000
更新日期:2000-04-01 00:00:00
abstract::The role of macrophages in protecting mice from murine cytomegalovirus (MCMV) was studied in Swiss, CBA/J, and C57BL/6J mice. CBA/J mice were more resistant to virus than were C57BL/6J mice at all ages tested. Prior treatment of adult Swiss mice with 60 mg of silica, a dose selectively toxic to macrophages, increased ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1383-1390.1974
更新日期:1974-12-01 00:00:00
abstract::Male ICR Swiss mice (2 to 3 months old) were fed Candida albicans in their drinking water for 3 days, followed by no treatment, antibiotics in their drinking water (daily), or immunosuppressants given by intraperitoneal injection (two to three times weekly) over a 3- to 4-week period. The organs of animals were proces...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1558-1563.1987
更新日期:1987-07-01 00:00:00
abstract::Cells infected by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, display unusual intracellular morphological changes characterized by dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope. These changes are consistent with those that might be expected to occur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3110-3115.1988
更新日期:1988-12-01 00:00:00
abstract::Nitric oxide (NO) produced by inducible NO synthase (iNOS) mediates hypotension in endotoxemia. In this study, NO induction by a toxin-producing Streptococcus pyogenes isolate, H250, and by recombinant streptococcal pyrogenic exotoxin A (rSPEA) has been examined, both in vitro and in vivo. Streptococcal supernatants, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1767-1772.1997
更新日期:1997-05-01 00:00:00
abstract::Cryptococcus neoformans is an opportunistic fungal pathogen that primarily causes disease in immunocompromised individuals. Dendritic cells (DCs) can phagocytose C. neoformans, present cryptococcal antigen, and kill C. neoformans. However, early events following C. neoformans phagocytosis by DCs are not well defined. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00660-08
更新日期:2008-10-01 00:00:00