Abstract:
:During the process of phagocytosis, human leukocytes emit a burst of luminescence which can be measured in a liquid scintillation spectrometer. The enzyme superoxide dismutase, which removes superoxide anions (O(2.)), inhibited this chemiluminescence by 70% at a concentration of 100 mug/ml. The enzyme did not inhibit phagocytosis. These results support other studies indicating that O(2.) is elaborated by phagocytizing leukocytes. They also indicate that O(2.) plays a major role in phagocytosis-associated chemiluminescence, though not necessarily as the luminescing agent. Catalase and benzoate inhibited the chemiluminescence of phagocytosis to a slight extent, suggesting that hydrogen peroxide and hydroxyl radical, respectively, might also be involved in this phenomenon. The relationship between the mediators of chemiluminescence and those responsible for phagocytic bactericidal activity remains to be defined.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Webb LS,Keele BB Jr,Johnston RB Jrsubject
Has Abstractpub_date
1974-06-01 00:00:00pages
1051-6issue
6eissn
0019-9567issn
1098-5522journal_volume
9pub_type
杂志文章abstract::Depletion of dendritic cells (DC) in secondary lymphoid organs is a hallmark of sepsis-induced immune dysfunction. In this setting, we investigated if Toll-like receptor (TLR)-dependent signaling might modulate the maturation process and the survival of DC. Using a model of sublethal polymicrobial sepsis induced by ce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00238-09
更新日期:2009-12-01 00:00:00
abstract::During persistence of nonencapsulated Haemophilus influenzae in the respiratory tracts of patients with chronic bronchitis, the major outer membrane proteins (MOMPs) P2 and P5 show antigenic drift. The hypothesis that appearance of antigenic variants is the consequence of antibody-dependent selection was tested in a r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.980-986.1996
更新日期:1996-03-01 00:00:00
abstract::Trypanosoma brucei subsp. brucei and T. congolense incubated in homologous antibody at 0 degrees C for 30 min were lysed by subsequent addition of guinea pig complement. Trypanosomes incubated in antibody at 37 degrees C before complement treatment remained intact. Parasites bearing adsorbed antibody also remained int...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.617-627.1979
更新日期:1979-06-01 00:00:00
abstract::Nonviable Mycobacterium tuberculosis strain Jamaica suspended in oil-droplet emulsions was used to enhance resistance of mice against encephalomyocarditis virus (EMCV). The mycobacteria-injected mice were significantly resistant to 50,000 50% lethal doses of EMCV. Similar concentrations of virus in plasma of normal an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.740-745.1978
更新日期:1978-12-01 00:00:00
abstract::The plasmid-encoded structural gene cofA necessary for the production of the major pilin subunit of pilus colonization factor antigen III (CFA/III) of human enterotoxigenic Escherichia coli was identified, and the nucleotide sequence of the gene was determined. cofA consists of 714 nucleotides encoding a 238-amino-aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.724-728.1995
更新日期:1995-02-01 00:00:00
abstract::Trypanosome-activated mouse peritoneal macrophages phagocytized and digested Trypanosoma brucei in vitro and in vivo, but in the absence of specific antiserum and complement the degree of phagocytosis was minimal. Ultrastructurally, the parasites attached to the macrophage by their flagella, and ingestion proceeded fl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.972-982.1978
更新日期:1978-03-01 00:00:00
abstract::Q fever has been associated with granulomatous changes in clinical biopsy material obtained from liver and bone marrow. Local reactions to skin testing have been described in previously sensitized humans, but histological studies of such reactions have not been reported. We note that delayed hypersensitivity reactions...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.1.388-393.1983
更新日期:1983-01-01 00:00:00
abstract::Group A streptococcal pyrogenic exotoxins A, B, and C (also known as scarlet fever toxins and erythrogenic toxins) were evaluated for relatedness to another streptococcus-derived lymphocyte mitogen, blastogen A. Streptococcal pyrogenic exotoxin A and blastogen A were immunologically cross-reactive and shared the same ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1865-1867.1989
更新日期:1989-06-01 00:00:00
abstract::Six strains of Rickettsia rickettsii from Montana and North Carolina were examined in an effort to identify rickettsial constituents associated with virulence. Fever responses, scrotal reactions, and mortalities of male guinea pigs inoculated intraperitoneally with 1,000 PFU of rickettsial strains revealed that the tw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.559-564.1984
更新日期:1984-06-01 00:00:00
abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4400-4406.1999
更新日期:1999-09-01 00:00:00
abstract::The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.547-550.2001
更新日期:2001-01-01 00:00:00
abstract::A preparation procedure for scanning electron microscopy was developed by which gononcoccal colonies can be studied directly on the agar surface. After glutaraldehyde fixation directly in petri dishes, small agar pieces were cut out and dehydrated stepwise in increasing concentrations of ethanol. The blocks were there...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.3.630-637.1975
更新日期:1975-09-01 00:00:00
abstract::A GroEL-like protein from Bordetella pertussis was purified. This protein was found to have the tetradecameric subunit structure typical of the GroEL family of proteins and to contain epitopes similar to those of other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1417-1422.1991
更新日期:1991-04-01 00:00:00
abstract::Vibrio cholerae V86 El Tor Inaba caused experimental cholera in germfree ICR suckling mice. Sucklings born of gnotobiotic mothers monoassociated with the same strain were completely protected against live oral homologous challenge. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.296-298.1981
更新日期:1981-10-01 00:00:00
abstract::The predominant gram-positive bacteria in 47 fecal specimens from 10 healthy men were studied by microscopic and cultural counts, by the characterization and tentative identification of isolates, and by the use of fluorescein isothiocyanate (FITC)-conjugated globulins prepared using some of the isolates. Gram-positive...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.4.719-729.1974
更新日期:1974-04-01 00:00:00
abstract::The bacterium Listeria monocytogenes causes meningoencephalitis in humans. In rodents, listeriosis is associated with granulomatous lesions in the liver and the spleen, but not with meningoencephalitis. Here, infant rats were infected intracisternally to generate experimental listeric meningoencephalitis. Dose-depende...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.4086-4093.2001
更新日期:2001-06-01 00:00:00
abstract::Extracytoplasmic proteins were released from Serpulina (Treponema) hyodysenteriae (strain B204) by treatment of whole cells with a nonionic detergent (Tween 20). Centrifugation of the Tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.142-148.1995
更新日期:1995-01-01 00:00:00
abstract::Factors which control the levels of catalase within yeast cells of Histoplasma capsulatum were studied. Only a small fraction of the total catalase activity could be detected in whole cells. The bulk of the activity was revealed in cell-free extracts or in cells permeabilized with acetone. The formation of the enzyme ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1161-1166.1983
更新日期:1983-03-01 00:00:00
abstract::Recombinant major surface antigen (P30), which was produced as a glutathione S-transferase (EC 2.5.1.18) fusion protein of Toxoplasma gondii, was found to be able to activate macrophages to kill T. gondii in vitro. The macrophage activation was due to P30 in the fusion protein, not to glutathione S-transferase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2851-2852.1991
更新日期:1991-08-01 00:00:00
abstract::Cells infected by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, display unusual intracellular morphological changes characterized by dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope. These changes are consistent with those that might be expected to occur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3110-3115.1988
更新日期:1988-12-01 00:00:00
abstract::As an opportunistic bacterial pathogen, Pseudomonas aeruginosa mainly affects immunocompromised individuals as well as patients with cystic fibrosis. In a previous study, we showed that ExoS of P. aeruginosa, when injected into host cells through a type III secretion apparatus, functions as an effector molecule to tri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3361-3370.2003
更新日期:2003-06-01 00:00:00
abstract::Isolated outer membranes of Borrelia burgdorferi 297 were utilized to obtain partial amino acid sequence information for a low-molecular-weight, outer membrane-associated polypeptide. Degenerate oligonucleotide primers based upon this information were used to amplify a 100-bp probe for detection of the corresponding f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.412-421.1997
更新日期:1997-02-01 00:00:00
abstract::Oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with Campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. Disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. Orally infected animals developed agglutinin titers of 8 to 256 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1848-1852.1990
更新日期:1990-06-01 00:00:00
abstract::Capsular glucuronoxylomannans (GXM) of Cryptococcus neoformans var. neoformans isolates from patients with recurrent cryptococcal meningitis were analyzed by 1H nuclear magnetic resonance spectroscopy and for reactivity with factor sera (Iatron, Tokyo, Japan). For each patient the initial and relapse isolates had prev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1899-1905.1995
更新日期:1995-05-01 00:00:00
abstract::The heat-labile enterotoxin (LT) of Escherichia coli is localized in the periplasm of the bacterial cell. Growth of an LT-producing strain of E. coli in the presence of bile salts, in concentrations within the range found in the human small intestine, caused leakage of LT into the culture medium. This leakage could be...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.1.168-171.1991
更新日期:1991-01-01 00:00:00
abstract::Borrelia burgdorferi synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which ospD is deleted, we d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00714-07
更新日期:2008-05-01 00:00:00
abstract::The results of this study show that recombinant interleukin-8 (IL-8) enhances the intracellular killing of Mycobacterium fortuitum by human granulocytes. This chemokine did not stimulate the phagocytosis of M. fortuitum by granulocytes at various bacterium-to-cell ratios. The killing process was not affected by the NA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3111-3116.1993
更新日期:1993-08-01 00:00:00
abstract::A 2.9-kilobase pair locus in Neisseria meningitidis was identified as containing transcriptionally linked open reading frames encoding TolC- and HlyD-like proteins. Although the meningococcal TolC protein was required for extracellular production of the repeats-in-toxin (RTX) FrpC toxin, it could not functionally repl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01995-06
更新日期:2007-12-01 00:00:00
abstract::The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00027-12
更新日期:2012-05-01 00:00:00
abstract::The combining sites of type 1 fimbrial lectins of various species of enterobacteria were studied by measuring the inhibitory activity of linear and branched oligosaccharides and several glycosides of D-mannose on the agglutination of yeast cells by the organisms. The results showed that all five strains of Escherichia...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1088-1090.1984
更新日期:1984-03-01 00:00:00