Abstract:
:gammadelta T cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterize gammadelta (T-cell receptor 1(+) [TCR1(+)]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar Enteritidis live vaccine or S. enterica serovar Enteritidis wild-type strain and infected using the S. enterica serovar Enteritidis wild-type strain on day 44 of life. Between days 3 and 71, peripheral blood was examined flow cytometrically for the occurrence of gammadelta T-cell subpopulations differentiated by the expression of T-cell antigens. Three different TCR1(+) cell populations were found to display considerable variation regarding CD8alpha antigen expression: (i) CD8alpha(+high) TCR1(+) cells, (ii) CD8alpha(+dim) TCR1(+) cells, and (iii) CD8alpha(-) TCR1(+) cells. While most of the CD8alpha(+high) TCR1(+) cells expressed the CD8alphabeta heterodimeric antigen, the majority of the CD8alpha(+dim) TCR1(+) cells were found to express the CD8alphaalpha homodimeric form. After immunization, a significant increase of CD8alphaalpha(+high) gammadelta T cells was observed within the CD8alpha(+high) TCR1(+) cell population. Quantitative reverse transcription-PCR revealed reduced interleukin-7 receptor alpha (IL-7Ralpha) and Bcl-x expression and elevated IL-2Ralpha mRNA expression of the CD8alphaalpha(+high) gammadelta T cells. Immunohistochemical analysis demonstrated a significant increase of CD8alpha(+) and TCR1(+) cells in the cecum and spleen and a decreased percentage of CD8beta(+) T cells in the spleen after Salmonella immunization. After infection of immunized animals, immune reactions were restricted to intestinal tissue. The study showed that Salmonella immunization of very young chicks is accompanied by an increase of CD8alphaalpha(+high) gammadelta T cells in peripheral blood, which are probably activated, and thus represent an important factor for the development of a protective immune response to Salmonella organisms in chickens.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Berndt A,Pieper J,Methner Udoi
10.1128/IAI.01128-05subject
Has Abstractpub_date
2006-07-01 00:00:00pages
3967-78issue
7eissn
0019-9567issn
1098-5522pii
74/7/3967journal_volume
74pub_type
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/IAI.65.4.1402-1407.1997
更新日期:1997-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.931-933.1975
更新日期:1975-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.16.1.213-217.1977
更新日期:1977-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.63.12.4812-4817.1995
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1996-04-01 00:00:00
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更新日期:2011-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4110-4116.1991
更新日期:1991-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1974-10-01 00:00:00