Circulating gamma delta T cells in response to Salmonella enterica serovar enteritidis exposure in chickens.

Abstract:

:gammadelta T cells are considered crucial to the outcome of various infectious diseases. The present study was undertaken to characterize gammadelta (T-cell receptor 1(+) [TCR1(+)]) T cells phenotypically and functionally in avian immune response. Day-old chicks were orally immunized with Salmonella enterica serovar Enteritidis live vaccine or S. enterica serovar Enteritidis wild-type strain and infected using the S. enterica serovar Enteritidis wild-type strain on day 44 of life. Between days 3 and 71, peripheral blood was examined flow cytometrically for the occurrence of gammadelta T-cell subpopulations differentiated by the expression of T-cell antigens. Three different TCR1(+) cell populations were found to display considerable variation regarding CD8alpha antigen expression: (i) CD8alpha(+high) TCR1(+) cells, (ii) CD8alpha(+dim) TCR1(+) cells, and (iii) CD8alpha(-) TCR1(+) cells. While most of the CD8alpha(+high) TCR1(+) cells expressed the CD8alphabeta heterodimeric antigen, the majority of the CD8alpha(+dim) TCR1(+) cells were found to express the CD8alphaalpha homodimeric form. After immunization, a significant increase of CD8alphaalpha(+high) gammadelta T cells was observed within the CD8alpha(+high) TCR1(+) cell population. Quantitative reverse transcription-PCR revealed reduced interleukin-7 receptor alpha (IL-7Ralpha) and Bcl-x expression and elevated IL-2Ralpha mRNA expression of the CD8alphaalpha(+high) gammadelta T cells. Immunohistochemical analysis demonstrated a significant increase of CD8alpha(+) and TCR1(+) cells in the cecum and spleen and a decreased percentage of CD8beta(+) T cells in the spleen after Salmonella immunization. After infection of immunized animals, immune reactions were restricted to intestinal tissue. The study showed that Salmonella immunization of very young chicks is accompanied by an increase of CD8alphaalpha(+high) gammadelta T cells in peripheral blood, which are probably activated, and thus represent an important factor for the development of a protective immune response to Salmonella organisms in chickens.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Berndt A,Pieper J,Methner U

doi

10.1128/IAI.01128-05

subject

Has Abstract

pub_date

2006-07-01 00:00:00

pages

3967-78

issue

7

eissn

0019-9567

issn

1098-5522

pii

74/7/3967

journal_volume

74

pub_type

杂志文章
  • Pathogenicity of Escherichia coli O157:H7 in the intestines of neonatal calves.

    abstract::Cattle are an important reservoir of Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains, foodborne pathogens that cause hemorrhagic colitis and hemolytic uremic syndrome in humans. EHEC O157:H7 strains are not pathogenic in calves >3 weeks old. Our objective was to determine if EHEC O157:H...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.5.1842-1848.1997

    authors: Dean-Nystrom EA,Bosworth BT,Cray WC Jr,Moon HW

    更新日期:1997-05-01 00:00:00

  • Genetic analysis of virulence plasmid from a serogroup 9 Yersinia enterocolitica strain: role of outer membrane protein P1 in resistance to human serum and autoagglutination.

    abstract::Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (70 kilobases) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of some outer membrane proteins (OMPs). To map the genes enco...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.3.782-786.1985

    authors: Balligand G,Laroche Y,Cornelis G

    更新日期:1985-06-01 00:00:00

  • Phagocytosis of microbial L-forms.

    abstract::Little data are available regarding L-form phagocyte interactions. Due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. Intracellular localization of L-f...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.6.976-981.1972

    authors: Harwick HJ,Barajas L,Montgomerie JZ,Kalmanson GM,Guze LB

    更新日期:1972-06-01 00:00:00

  • Antigenic characterization of an intrinsically unstructured protein, Plasmodium falciparum merozoite surface protein 2.

    abstract::Merozoite surface protein 2 (MSP2) is an abundant glycosylphosphatidylinositol (GPI)-anchored protein of Plasmodium falciparum, which is a potential component of a malaria vaccine. As all forms of MSP2 can be categorized into two allelic families, a vaccine containing two representative forms of MSP2 may overcome the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00665-12

    authors: Adda CG,MacRaild CA,Reiling L,Wycherley K,Boyle MJ,Kienzle V,Masendycz P,Foley M,Beeson JG,Norton RS,Anders RF

    更新日期:2012-12-01 00:00:00

  • Levels of salivary lysozyme, lactoperoxidase, and lactoferrin in diabetic hamsters.

    abstract::In an attempt to clarify the mechanism(s) of increased susceptibility to oral infection in diabetics, we examined the levels of salivary antibacterial factors, including lysozyme, lactoperoxidase, and lactoferrin, in diabetic hamsters whose condition was induced with streptozotocin. Saliva was collected from these ham...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.2.389-394.1985

    authors: Muratsu K,Morioka T

    更新日期:1985-05-01 00:00:00

  • Opportunist mycobacteria express ligands that stimulate production of human V gamma 9V delta 2 T lymphocytes.

    abstract::Human gamma delta T cells are known to respond at high frequencies to pathogenic mycobacteria. Here we show that opportunistic strains of mycobacteria share with pathogenic mycobacteria the ability to trigger at high frequencies human V gamma 9V delta 2 T-cell-receptor-positive T lymphocytes. Stimulating ligands were ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.7.2753-2757.1992

    authors: Häcker G,Kromer S,Heeg K,Ivanyi J,Wagner H,Pfeffer K

    更新日期:1992-07-01 00:00:00

  • Leukotoxin confers beta-hemolytic activity to Actinobacillus actinomycetemcomitans.

    abstract::Actinobacillus actinomycetemcomitans is the etiologic agent of localized aggressive periodontitis, a rapidly progressing oral disease that occurs in adolescents. A. actinomycetemcomitans can also cause systemic disease, including infective endocarditis. In early work on A. actinomycetemcomitans workers concluded that ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.74.4.2015-2021.2006

    authors: Balashova NV,Crosby JA,Al Ghofaily L,Kachlany SC

    更新日期:2006-04-01 00:00:00

  • Localization of electron-dense tracers during entry of Rickettsia tsutsugamushi into polymorphonuclear leukocytes.

    abstract::The invasion of Rickettsia tsutsugamushi, Gilliam strain, into guinea pig polymorphonuclear leukocytes (PMNs) and the localization and distribution of tracers were followed during the process by electron microscopy. The seven tracers used were: cationized ferritin, ferritin, thorium dioxide (ThO2), carbon particles, l...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.30.1.231-243.1980

    authors: Rikihisa Y,Ito S

    更新日期:1980-10-01 00:00:00

  • Rickettsial infectious antibody complexes: detection by antiglobulin plaque reduction technique.

    abstract::Rickettsia rickettsii and R. australis formed infectious antibody complexes when reacted with homologous convalescent serum. The addition of antiglobulin to these complexes showed neutralization of plaques proportional to the concentration of rickettsial immune serum. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.9.5.966-968.1974

    authors: Kenyon RH,McManus AT

    更新日期:1974-05-01 00:00:00

  • Host defense against cholera toxin is strongly CD4+ T cell dependent.

    abstract::This study investigates the role of CD4+ T cells in host defense against cholera enterotoxin-induced diarrhea. Antitoxin immunoglobulin A formation and gut protection against cholera toxin (CT) following oral immunizations with CT were evaluated in normal mice and mice that had been depleted of CD4+ T cells by in vivo...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.10.3630-3638.1991

    authors: Hörnqvist E,Goldschmidt TJ,Holmdahl R,Lycke N

    更新日期:1991-10-01 00:00:00

  • Antibody-mediated protection against infection with Helicobacter pylori in a suckling mouse model of passive immunity.

    abstract::Studies of active immunization against Helicobacter pylori indicate that antibodies play a minor role in immunity. There is also evidence, however, that the translocation of antibodies in the stomach may be insufficient to achieve functional antibody levels in the gastric lumen. We have used a suckling mouse model of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00547-09

    authors: Gorrell RJ,Robins-Browne RM

    更新日期:2009-11-01 00:00:00

  • Production of a complete binary toxin (actin-specific ADP-ribosyltransferase) by Clostridium difficile CD196.

    abstract::A Clostridium difficile isolate was found to produce an actin-specific ADP-ribosyltransferase (CDT) homologous to the enzymatic components of Clostridium perfringens iota toxin and Clostridium spiroforme toxin (M. R. Popoff, E. J. Rubin, D. M. Gill, and P. Boquet, Infect. Immun. 56:2299-2306, 1988). The CDT locus from...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.4.1402-1407.1997

    authors: Perelle S,Gibert M,Bourlioux P,Corthier G,Popoff MR

    更新日期:1997-04-01 00:00:00

  • Inhibition of hemolytic activity of the thermostable direct hemolysin of Vibrio parahaemolyticus by ganglioside.

    abstract::The hemolytic activity of the thermostable direct hemolysin produced by Vibrio parahaemolyticus was inhibited by a ganglioside mixture. The ganglioside component which inhibited the hemolysin was not FM1 ganglioside. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.12.4.931-933.1975

    authors: Takeda R,Honda T,Sakural J,Otomo N

    更新日期:1975-10-01 00:00:00

  • Virulence of Pseudomonas aeruginosa in a murine model of gastrointestinal colonization and dissemination in neutropenia.

    abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.4.2262-2272.2005

    authors: Koh AY,Priebe GP,Pier GB

    更新日期:2005-04-01 00:00:00

  • Protective role of complement in experimental Escherichia coli endocarditis.

    abstract::Fourteen strains of Escherichia coli were tested for ability to cause infective endocarditis in rabbits prepared by prior placement of an intracardiac catheter. Strains that were resistant to the bactericidal action of serum caused E. coli endocarditis in 91.4% of rabbits, whereas serum-sensitive strains usually faile...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.16.1.213-217.1977

    authors: Durack DT,Beeson PB

    更新日期:1977-04-01 00:00:00

  • Agonists of Toll-like receptors 3, 4, 7, and 9 are candidates for use as adjuvants in an outer membrane vaccine against Neisseria meningitidis serogroup B.

    abstract::The bacterium Neisseria meningitidis is the causative agent of meningitis and sepsis. A generally effective vaccine against N. meningitidis serogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion of N. meningitidis wild-ty...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00846-07

    authors: Fransen F,Boog CJ,van Putten JP,van der Ley P

    更新日期:2007-12-01 00:00:00

  • Prevention of endotoxin-induced acute lethality in Propionibacterium acnes-primed rabbits by an antibody to leukocyte integrin beta 2 with concomitant reduction of cytokine production.

    abstract::Acute lethality was induced in rabbits by the sequential injection of Propionibacterium acnes and lipopolysaccharide (LPS). P. acnes induced the infiltration of inflammatory cells into the liver lobules during the early phase, and LPS in the late phase caused death in association with pathological changes mimicking he...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.12.4812-4817.1995

    authors: Ikeda N,Mukaida N,Kaneko S,Fujioka N,Su S,Nariuchi H,Unoura M,Harada K,Nakanuma Y,Kobayashi K

    更新日期:1995-12-01 00:00:00

  • Periplasmic superoxide dismutase in meningococcal pathogenicity.

    abstract::Meningococcal sodC encodes periplasmic copper- and zinc-cofactored superoxide dismutase (Cu,Zn SOD) which catalyzes the conversion of the superoxide radical anion to hydrogen peroxide, preventing a sequence of reactions leading to production of toxic hydroxyl free radicals. From its periplasmic location, Cu,Zn SOD was...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.1.213-217.1998

    authors: Wilks KE,Dunn KL,Farrant JL,Reddin KM,Gorringe AR,Langford PR,Kroll JS

    更新日期:1998-01-01 00:00:00

  • Pathogenicity of stable L-phase variants of Staphylococcus aureus: failure to colonize experimental endocarditis in rabbits.

    abstract::Endocarditis was induced in the rabbit by the placement of a polyethylene catheter in the right heart. The catheter was filled with stable L-phase variants of Staphylococcus aureus to determine if the variant form would colonize the damaged endocardium and produce further tissue injury similar to that produced by the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.7.5.725-730.1973

    authors: Linnemann CC Jr,Watanakunakorn C,Bakie C

    更新日期:1973-05-01 00:00:00

  • Immunodiffusion analysis of membranes of Thermoplasma acidophilum.

    abstract::Isolates of Thermoplasma acidophilum were divided into serological groups based on immunodiffusion studies of solubilized-membrane preparations. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.1.280-281.1974

    authors: Bohlool BB,Brock TD

    更新日期:1974-07-01 00:00:00

  • Expression of the Haemophilus influenzae transferrin receptor is repressible by hemin but not elemental iron alone.

    abstract::The absolute requirement for elemental iron and the porphyrin nucleus for growth of Haemophilus influenzae led us to investigate the role of iron and hemin in regulation of expression of the H. influenzae transferrin receptor. H. influenzae type b strain H1689 was grown in brain heart infusion broth supplemented with ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.10.4033-4037.1993

    authors: Morton DJ,Musser JM,Stull TL

    更新日期:1993-10-01 00:00:00

  • Effect of cimetidine and antacid on gastric microbial flora.

    abstract::The effect of a standard regimen of cimetidine on the gastric flora of 20 male volunteers was studied in a double-blind manner and compared with the effects of a standard antacid regimen. Postprandial microbial titers in gastric aspirates were significantly higher at 4, 8, and 16 weeks of therapy in subjects taking an...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1128/IAI.36.2.518-524.1982

    authors: Snepar R,Poporad GA,Romano JM,Kobasa WD,Kaye D

    更新日期:1982-05-01 00:00:00

  • Avirulent uracil auxotrophs based on disruption of orotidine-5'-monophosphate decarboxylase elicit protective immunity to Toxoplasma gondii.

    abstract::The orotidine-5'-monophosphate decarboxylase (OMPDC) gene, encoding the final enzyme of the de novo pyrimidine biosynthesis pathway, was deleted using Toxoplasma gondii KU80 knockouts to develop an avirulent nonreverting pyrimidine auxotroph strain. Additionally, to functionally address the role of the pyrimidine salv...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00287-10

    authors: Fox BA,Bzik DJ

    更新日期:2010-09-01 00:00:00

  • Oral vaccination of weaned rabbits against enteropathogenic Escherichia coli-like E. coli O103 infection: use of heterologous strains harboring lipopolysaccharide or adhesin of pathogenic strains.

    abstract::To test the importance of lipopolysaccharide (LPS) and adhesin as major antigens in vaccination against rabbit enteropathogenic Escherichia coli (EPEC)-like E. coli O103 infection, we used two nonpathogenic wild-type strains to immunize rabbits at weaning. One of these strains (C127) harbors the O103 LPS but does not ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.7.2702-2709.1992

    authors: Milon A,Esslinger J,Camguilhem R

    更新日期:1992-07-01 00:00:00

  • Immunogenicity of liposomes and iscoms containing the major outer membrane protein of Neisseria gonorrhoeae: influence of protein content and liposomal bilayer composition.

    abstract::The influences of Neisseria gonorrhoeae protein IB content and bilayer composition of liposomes and protein content of iscoms on immunogenicity were investigated. Changes in the protein content of liposomes did not influence the immunoglobulin G response, whereas the response was lowered when the amount of protein in ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.6.1661-1664.1988

    authors: Kersten GF,van de Put AM,Teerlink T,Beuvery EC,Crommelin DJ

    更新日期:1988-06-01 00:00:00

  • Delayed mortality and attenuated thrombocytopenia associated with severe malaria in urokinase- and urokinase receptor-deficient mice.

    abstract::We explored the role of urokinase and tissue-type plasminogen activators (uPA and tPA), as well as the uPA receptor (uPAR; CD87) in mouse severe malaria (SM), using genetically deficient (-/-) mice. The mortality resulting from Plasmodium berghei ANKA infection was delayed in uPA(-/-) and uPAR(-/-) mice but was simila...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.7.3822-3829.2000

    authors: Piguet PF,Da Laperrousaz C,Vesin C,Tacchini-Cottier F,Senaldi G,Grau GE

    更新日期:2000-07-01 00:00:00

  • Salivary, nasal, genital, and systemic antibody responses in monkeys immunized intranasally with a bacterial protein antigen and the Cholera toxin B subunit.

    abstract::Previous attempts to induce mucosal antibodies in rhesus monkeys by enteric immunization have resulted in only modest and short-lived responses, dominated by immunoglobulin M (IgM) antibodies in the plasma. In this study, two groups of rhesus monkeys were immunized intranasally three times at 2-week intervals with a b...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.4.1272-1283.1996

    authors: Russell MW,Moldoveanu Z,White PL,Sibert GJ,Mestecky J,Michalek S M

    更新日期:1996-04-01 00:00:00

  • Necessary and sufficient role for T helper cells to prevent fungal dissemination in allergic lung disease.

    abstract::Mucosal immune responses to fungal infection range from T helper type 2 (Th2) cell-directed allergic inflammation to Th1-predominant neutrophilic inflammation, but the mechanisms directing these divergent mucosal immune outcomes and the role of T cells in host defense against mucosal fungal infections are not known. H...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.05209-11

    authors: Porter PC,Roberts L,Fields A,Knight M,Qian Y,Delclos GL,Han S,Kheradmand F,Corry DB

    更新日期:2011-11-01 00:00:00

  • Isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of Actinobacillus pleuropneumoniae serotype 7.

    abstract::Actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). The phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal DNA. A genomic fragment encoding the cytolysin and its flanking sequences was cloned ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.11.4110-4116.1991

    authors: Anderson C,Potter AA,Gerlach GF

    更新日期:1991-11-01 00:00:00

  • Immune responses in infections with coccidia: macrophage activity.

    abstract::Peritoneal exudate cells from chickens immunized with two species of coccidia, Eimeria tenella or Eimeria maxima, were examined for their capacity to phagocytose stages of the parasite in vitro. True phagocytosis of the sporozoite stage is difficult to estimate because of its ability to invade cells, but may be evalua...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.4.862-871.1974

    authors: Rose ME

    更新日期:1974-10-01 00:00:00