Abstract:
:The bactericidal activity of guinea pig and human polymorphonuclear leukocytes (PMNs) against a smooth-intermediate strain (45/0) and a rough strain (45/20) of Brucella abortus has been examined. After incubation for 120 min, guinea pig PMNs incubated with either the smooth strain 45/0 or the rough strain 45/20 exhibited no bactericidal activity against the former and caused only a 34% decrease in viability of the latter. Human PMNs were more bactericidal than guinea pig PMNs to both strains; however, the killing of strain 45/20 by human PMNs was less than that observed in control experiments with S. aureus strain 502A. Both strains of B. abortus readily associated with guinea pig and human PMNs, and the bacteria were apparently ingested without stimulation of the hexose monophosphate pathway. Lysates (10 micrograms/ml, pH 5.5), prepared from guinea pig or human granules, were not particularly toxic to either strain unless supplemented with H2O2 and a halide (I- or Cl-). An oxygen-dependent killing system appeared to be lethal against both strains of B. abortus, with I- being more active than Cl- in the presence of H2O2 and granule lysate. The data suggest that degranulation after ingestion of Brucella by phagocytes does not occur due to the lack of a proper stimulus or possibly the baccilli actively inhibit the degranulation process thereby protecting the microbe from killing systems normally effective against extracellular parasites.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kreutzer DL,Dreyfus LA,Robertson DCdoi
10.1128/IAI.23.3.737-742.1979keywords:
subject
Has Abstractpub_date
1979-03-01 00:00:00pages
737-42issue
3eissn
0019-9567issn
1098-5522journal_volume
23pub_type
杂志文章abstract::Pneumolysin is an important virulence factor of Streptococcus pneumoniae, interacting with the membranes of host cells to elicit a multitude of inflammatory responses. We used cDNA microarrays to identify genes which are responsive to S. pneumoniae in a pneumolysin-dependent and -independent fashion. The THP-1 human m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2087-2094.2003
更新日期:2003-04-01 00:00:00
abstract::Subpopulations of spleen cells from scrapie virus-infected mice were used to determine the average virus content of infected cells in vivo at a time when virus was rapidly increasing in titer in lymphoreticular tissues. Comparison of the mean lethal doses of lysed to intact cells indicated averages of 2 to 6 infectiou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.6.918-921.1973
更新日期:1973-06-01 00:00:00
abstract::Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00139-16
更新日期:2016-06-23 00:00:00
abstract::An immunosuppressed rat model of Pneumocystis carinii pneumonia is described that utilizes simple, noninvasive intratracheal (i.t.) inoculation of cryopreserved parasites and results in development of severe P. carinii pneumonia within 5 weeks. This is an improvement over the most commonly used models of P. carinii pn...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1589-1597.1992
更新日期:1992-04-01 00:00:00
abstract::The mechanism of adsorption of the Streptococcus mutans enzymes responsible for the synthesis of insoluble dextran-levan to the S. mutans cell-wall binding sites has been studied. Certain characteristics of these binding sites are presented. The adsorption of these enzymes to the cell surface occurred rapidly without ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.419-429.1974
更新日期:1974-02-01 00:00:00
abstract::Human genital Chlamydia infection is a major public health concern due to the serious reproductive system complications. Chlamydia binds several receptor tyrosine kinases (RTKs) on host cells, including the epidermal growth factor receptor (EGFR), and activates cellular signaling cascades for host invasion, cytoskelet...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00819-19
更新日期:2020-03-23 00:00:00
abstract::In this report, we demonstrate that the variable antigens present on the surface of Plasmodium knowlesi-infected erythrocytes could not be found on the surface of merozoites. A number of technical problems had to be solved to make such a comparative study possible, including the purification of merozoites by affinity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.275-284.1981
更新日期:1981-07-01 00:00:00
abstract::Streptococcus pneumoniae type 14 capsular polysaccharide-bovine serum albumin (S14PS-BSA) conjugates were prepared by water-soluble-carbodiimide-mediated condensation with or without the use of N-hydroxy-sulfosuccinimide. The immunogenicities of the capsular polysaccharide (S14PS) and of the conjugates were studied in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1078-1083.1989
更新日期:1989-04-01 00:00:00
abstract::A zone of extracellular digestion of the mucin layer around Candida albicans blastoconidia was observed by transmission electron microscopy in the jejunum of mice inoculated intragastrically (G. T. Cole, K. R. Seshan, L. M. Pope, and R. J. Yancey, J. Med. Vet. Mycol. 26:173-185, 1988). This observation prompted the hy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.11.4514-4519.1996
更新日期:1996-11-01 00:00:00
abstract::We used inhibitors of receptor-mediated endocytosis to study the mechanisms of infectivity, especially the uptake mechanism, of Chlamydia trachomatis for cultured cells. The effect of methylamine and monodansylcadaverine on the different stages of the chlamydial growth cycle in McCoy cells was examined. There was a do...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.534-541.1983
更新日期:1983-05-01 00:00:00
abstract::Porphyromonas gingivalis is a major etiologic agent of periodontitis, a chronic inflammatory disease that ultimately results in the loss of the supporting tissues of the teeth. Previous work has demonstrated the usefulness of avirulent Salmonella enterica serovar Typhimurium strains as antigen delivery systems for pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.732-739.2000
更新日期:2000-02-01 00:00:00
abstract::Clostridium perfringens delta toxin was selectively cytotoxic for various rabbit leukocyte populations. The sensitivity of these populations to the toxin varied, depending on the tissue from which they were derived, from 28% (appendix) to 41% (bone marrow) and 32% (spleen). Macrophages were uniformly killed by delta t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.860-864.1982
更新日期:1982-12-01 00:00:00
abstract::We recently determined that passive transfer of serum directed against a synthetic peptide called LB1 or a recombinant fusion protein immunogen [LPD-LB1(f)(2,1,3)] could prevent otitis media after challenge with a homologous nontypeable Haemophilus influenzae (NTHI) isolate. NTHI residing in the nasopharynx was rapidl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2756-2765.2000
更新日期:2000-05-01 00:00:00
abstract::Porphyromonas gingivalis is reportedly capable of stimulating the expression of host cell matrix metalloproteinases (MMP), contributing to tissue destruction. However, the impact of this bacterium on specific molecules remains to be determined. In this study, we evaluate the effect of P. gingivalis on regulation of MM...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.4940-4945.1996
更新日期:1996-12-01 00:00:00
abstract::Glucan-binding protein B (GbpB) from Streptococcus mutans has been shown to induce protective immunity to dental caries in experimental models. Having recently sequenced the gbpB gene, our objective in this study was to identify immunogenic regions within the GbpB sequence for use in subunit vaccines. Potential region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1179-1184.2003
更新日期:2003-03-01 00:00:00
abstract::We have previously shown that infection by Trypanosoma cruzi, a parasitic protozoan, is reduced by injection of CD40 ligand (CD40L)-transfected 3T3 fibroblasts (D. Chaussabel, F. Jacobs, J. de Jonge, M. de Veerman, Y. Carlier, K. Thielemans, M. Goldman, and B. Vray, Infect. Immun. 67:1929-1934, 1999). This prompted us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6552-6561.2005
更新日期:2005-10-01 00:00:00
abstract::Cell-mediated immunity is critical in host resistance against the pathogenic fungus Histoplasma capsulatum. To explore the role of L3T4+ T cells in protection of mice against H. capsulatum infection, we examined the effect of in vivo treatment with anti-L3T4 monoclonal antibody (MAb) GK1.5 on the course of murine diss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1685-1691.1988
更新日期:1988-07-01 00:00:00
abstract::The naturally antibiotic-resistant bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a disease with stubbornly high mortality and a complex, protracted treatment regimen. The worldwide incidence of melioidosis is likely grossly underreported, though it is known to be highly endemic in northern...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00938-17
更新日期:2018-04-23 00:00:00
abstract::Antimicrobial factors were analyzed in samples of whole saliva from 31 children, aged 0.8 to 3.8 years. When compared with the adult reference group, the children displayed similar levels of lysozyme, salivary peroxidase, and hypothiocyanite (OSCN-), whereas the amounts of immunoglobulins (isotypes A, G, and M), lacto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.49-53.1986
更新日期:1986-01-01 00:00:00
abstract::Outer membrane protein F (porin) was purified by extraction from polyacrylamide gels of cell envelope proteins of the Pseudomonas aeruginosa PAO1 strain. Rats were immunized intramuscularly with 25 micrograms of protein F on days 1 and 14 and then challenged on day 28 via intratracheal inoculation of bacterium-contain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1017-1022.1988
更新日期:1988-05-01 00:00:00
abstract::Carbon storage regulator A (CsrA) is an RNA binding protein that has been characterized in many bacterial species to play a central regulatory role by modulating several metabolic processes. We recently showed that a homolog of CsrA in Borrelia burgdorferi (CsrA(Bb), BB0184) was upregulated in response to propagation ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00882-10
更新日期:2011-02-01 00:00:00
abstract::The tetraspanins are a superfamily of transmembrane proteins with diverse functions and can form extended microdomains within the plasma membrane in conjunction with partner proteins, which probably includes receptors for bacterial adhesins. Neisseria meningitidis, the causative agent of meningococcal disease, attache...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01354-10
更新日期:2011-06-01 00:00:00
abstract::Encapsulated cells of Cryptococcus neoformans are potent activators of the alternative complement pathway. Previous studies found that monoclonal antibodies (MAbs) specific for the major capsular polysaccharide, termed glucuronoxylomannan (GXM), can markedly suppress the ability of the capsule to accumulate C3 from no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1547-1553.1998
更新日期:1998-04-01 00:00:00
abstract::Light and electron microscopy with histochemical staining were used to estimate exopolysaccharide production by strains of viridans streptococci recovered from patients with endocarditis. Six strains were selected for study because they represented a wide range of in vitro polysaccharide production. By light microscop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.1.359-367.1984
更新日期:1984-01-01 00:00:00
abstract::The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T-cell and B-cell epitopes as the constituents of a single immunogen. Here we report on the design, chemical synthesis, and immunogenicity of three Plasmodium falciparum MAP vaccines that incorporated antig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-10
更新日期:2010-11-01 00:00:00
abstract::The importance of bacterial phospholipases during serum-mediated killing of Escherichia coli was examined by using wild-type DR+ DS+ and an isogenic phospholipase-deficient mutant DR- DS-. No difference in serum sensitivity was observed when the parental DR+ DS+ and mutant DR- DS- strains were exposed to various conce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.183-188.1977
更新日期:1977-10-01 00:00:00
abstract::We determined the heavy (H)- and light (L)-chain variable (V) region nucleotide and translated amino acid sequences of the human immunoglobulin M(kappa) monoclonal antibody (MAb) 5E1, which is specific for the polysaccharide capsule of Escherichia coli K1 and Neisseria meningitidis group B (poly[alpha(2-->8)-N-acetyln...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1776-1786.1994
更新日期:1994-05-01 00:00:00
abstract::Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that whe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05048-11
更新日期:2011-09-01 00:00:00
abstract::Temperate bacteriophage can transfer toxin-encoding genes between bacteria, often resulting in acquired pathogenicity. However, little is known regarding the effects of the eukaryotic host on the phage-pathogen interaction. Using Streptococcus pyogenes as a model, we demonstrate, both in vitro and in vivo, that the eu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3782-3786.2003
更新日期:2003-07-01 00:00:00
abstract::Immunoglobulin G (IgG)- and IgM-specific antibody titers against Salmonella typhi Ty2 porins have been measured in 30 paired typhoid sera by enzyme-linked immunosorbent assay. These studies have found that IgG serum titers of acute and convalescent sera were 625 and 5,000 times higher, respectively than the control se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.209-212.1986
更新日期:1986-04-01 00:00:00