Abstract:
:Porphyromonas gingivalis is reportedly capable of stimulating the expression of host cell matrix metalloproteinases (MMP), contributing to tissue destruction. However, the impact of this bacterium on specific molecules remains to be determined. In this study, we evaluate the effect of P. gingivalis on regulation of MMP-9 expression in human gingival epithelial cells (HGEC). Various inocula of P. gingivalis were added to cultures of HGEC. The effects of live bacteria, heat-killed bacteria, and outer membrane extract were analyzed. MMP-9 secretion by HGEC was evaluated by enzyme-linked immunosorbent assay. For inocula smaller than one bacterium per cell, the quantity of MMP-9 secreted by HGEC was increased in comparison to control conditions. For inocula from 2.5 to 250 bacteria per cell, an inhibition of MMP-9 secretion in a dose-response fashion was observed, with a maximum reduction (ranging from 80 to 95% in five experiments) at 50 bacteria per cell. Gelatin zymograms confirmed the decrease in MMP-9 secretion. A band of 83 kDa, corresponding to activated enzyme, was present for inocula of 0.5 to 50 bacteria. Inhibition took place without any alteration of epithelial cell viability. Heat-killed bacteria and outer membrane extract also provoked proenzyme activation but did not inhibit MMP-9 secretion. These results demonstrate a direct effect of P. gingivalis on HGEC, suggesting a specific action on the collagen renewal process at the interface between the epithelium and connective tissue.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Fravalo P,Ménard C,Bonnaure-Mallet Mdoi
10.1128/IAI.64.12.4940-4945.1996subject
Has Abstractpub_date
1996-12-01 00:00:00pages
4940-5issue
12eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::Autotransporters of gram-negative bacteria are single-peptide secretion systems that consist of a functional N-terminal alpha-domain ("passenger") fused to a C-terminal beta-domain ("translocator"). How passenger proteins are translocated through the outer membrane has not been resolved, and at present essentially thr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.3851-3859.2005
更新日期:2005-07-01 00:00:00
abstract::The reproducibility of an experimental result is a fundamental assumption in science. Yet, results that are merely confirmatory of previous findings are given low priority and can be difficult to publish. Furthermore, the complex and chaotic nature of biological systems imposes limitations on the replicability of scie...
journal_title:Infection and immunity
pub_type: 社论
doi:10.1128/IAI.00908-10
更新日期:2010-12-01 00:00:00
abstract::The dispersed gene family 1 (DGF-1) is the fifth largest gene family in the Trypanosoma cruzi genome, with over 500 members (11). Many of the predicted DGF-1 protein products have several transmembrane domains and N-glycosylation and phosphorylation sites and were thought to localize in the plasma membrane. Here, we r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00780-09
更新日期:2010-01-01 00:00:00
abstract::The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00911-12
更新日期:2012-12-01 00:00:00
abstract::Thrombospondin-related anonymous protein (TRAP) of the malaria parasite Plasmodium falciparum shares two sequence motifs with other proteins which possess adhesive properties. Recently, findings indicate that TRAP is an antigen which contributes to antisporozoite immunity. We have cloned and expressed the TRAP coding ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3490-3495.1993
更新日期:1993-08-01 00:00:00
abstract::Susceptibility to Coccidioides spp. varies widely in humans and other mammals and also among individuals within a species. Among strains of mice with various susceptibilities, immunohistopathology revealed that C57BL/6 mice were highly susceptible to the disease following intranasal infection, DBA/2n mice were interme...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00885-08
更新日期:2008-12-01 00:00:00
abstract::Quorum-sensing systems are critical regulators of the expression of virulence factors of various organisms, including Pseudomonas aeruginosa. Las and Rhl are two major quorum-sensing components, and they are regulated by their corresponding autoinducers, N-3-oxododecanoyl homoserine lactone (3-oxo-C(12)-HSL) and N-but...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5785-5793.2003
更新日期:2003-10-01 00:00:00
abstract::Lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age-groups is urgent. Concerns ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00585-20
更新日期:2020-12-14 00:00:00
abstract::The cell surface hydrophobicity of 10 pigmented and 4 nonpigmented clinical Serratia marcescens strains was studied, based on the ability of the strains to adhere to hydrocarbons and to polystyrene. The cell surface hydrophobicity depended greatly on growth temperature; all of the strains tested were adherent followin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.932-935.1986
更新日期:1986-03-01 00:00:00
abstract::Three monoclonal antibodies (MAbs), all of the immunoglobulin G1 subclass, were raised against Cryptococcus neoformans by using the technique of cyclophosphamide ablation of B-cell responses against shared epitopes of the cross-reactive fungus Trichosporon beigelii. MAb 3C2 was reactive against the encapsulated and no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.143-149.1992
更新日期:1992-01-01 00:00:00
abstract::Alternate modalities for the treatment of Mycobacterium tuberculosis are needed due to the rise in numbers of immunosuppressed individuals at risk for serious disease and the increasing prevalence of multidrug-resistant isolates. Interleukin-12 (IL-12) has been shown to improve immune responses against M. tuberculosis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2976-2988.2004
更新日期:2004-05-01 00:00:00
abstract::V beta 10(+) and V beta 14(+) T cells were selectively increased 7 to 14 days following infection in the lungs of naive mice infected with Histoplasma capsulatum. Following secondary challenge of immune mice, V beta 1(+) and V beta 8.1(+) cells were sporadically increased. Elimination of V beta 10(+) and V beta 14(+) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1648-1652.2002
更新日期:2002-03-01 00:00:00
abstract::Salmonella serovars are associated with human diseases that range from mild gastroenteritis to host-disseminated enteric fever. Human infections by Salmonella enterica serovar Typhi can lead to typhoid fever, but this serovar does not typically cause disease in mice or other animals. In contrast, S. enterica serovar T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1005-1013.2000
更新日期:2000-03-01 00:00:00
abstract::The etiology of recurrent vulvovaginal candidiasis in otherwise healthy women of child-bearing age remains an enigma. To date, results from both clinical studies and a murine model of vaginal candidiasis indicate that Candida vaginitis can occur in the presence of Candida-specific Th1-type cell-mediated immunity expre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2403-2408.1995
更新日期:1995-07-01 00:00:00
abstract::The pathogenicities of three proteases from Serratia marcescens, two proteases from Pseudomonas aeruginosa, and one thermolysin from Bacillus stearothermophilus were examined. All proteases tested caused acute liquefactive necrosis of the cornea and descemetocele formation in guinea pig eyes after intrastromal injecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2509-2517.1987
更新日期:1987-10-01 00:00:00
abstract::A clinically isolated strain of polymorphonuclear leukocyte (PMN)-resistant Pseudomonas was found to produce an extracellular substance (PMN inhibitor) that inhibits the phagocytic and killing activities of PMN. The PMN inhibitor was purified from culture filtrates by precipitation with (NH4)2SO4 and chromatography on...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.394-398.1979
更新日期:1979-05-01 00:00:00
abstract::Neisseria meningitidis is the causative agent of meningococcal disease. Onset of meningococcal disease can be extremely rapid and can kill within a matter of hours. However, although a much-feared pathogen, Neisseria meningitidis is frequently found in the nasopharyngeal mucosae of healthy carriers. The bacterial fact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02001-14
更新日期:2014-12-01 00:00:00
abstract::Piglets suckled by dams that had been vaccinated with K88 antigen were significantly more resistant to deaths caused by neonatal diarrhea after challenge with a large dose of a K88-positive enteropathogenic strain of Escherichia coli than piglets suckled by control dams. The factors most likely to be involved in prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.667-676.1976
更新日期:1976-03-01 00:00:00
abstract::The ocular damage in young adult mice produced by purified Pseudomonas aeruginosa exotoxin A was microscopically characterized at 1 and 5 h and at 1, 3, 5, 7, 10, 14, and 21 days after toxin A challenge, using light, transmission, and scanning electron microscopic techniques. Similarly to previously described infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.1025-1035.1981
更新日期:1981-12-01 00:00:00
abstract::Resequencing estA3, an allele of the methanol-soluble heat-stable enterotoxin of Escherichia coli showed that the proline triplet 19 is in fact an alanine codon; thus, estA alleles 3 and 4 were shown to be identical. Resequencing has also shown that the carboxy terminus of another allele, estA2, is not the previously ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.645-648.1989
更新日期:1989-02-01 00:00:00
abstract::Suitability of the rhesus monkey (Macaca mulatta) as an experimental host for evaluation of vaccines against airborne infection with Mycobacterium tuberculosis strain H37Rv was investigated. Nonvaccinated monkeys were exposed to estimated doses of 12, 25, or 49 units of H37Rv in a modified Henderson apparatus, and the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.574-582.1970
更新日期:1970-11-01 00:00:00
abstract::gamma delta T cells have variously been implicated in the protection against, and the pathogenesis of, malaria, but few studies have examined the gamma delta T-cell response to malaria in African children, who suffer the large majority of malaria-associated morbidity and mortality. This is unfortunate, since available...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3190-3196.2001
更新日期:2001-05-01 00:00:00
abstract::A chimeric recombinant protein composed of the lktA gene product from Pasteurella haemolytica fused to bovine interleukin-2 (IL-2) was made. The LKT-IL-2 chimera was compared with recombinant bovine IL-2 with regard to the ability to induce proliferative responses and LAK cell activity in bovine peripheral blood monon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.565-570.1992
更新日期:1992-02-01 00:00:00
abstract::Extracytoplasmic proteins were released from Serpulina (Treponema) hyodysenteriae (strain B204) by treatment of whole cells with a nonionic detergent (Tween 20). Centrifugation of the Tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.142-148.1995
更新日期:1995-01-01 00:00:00
abstract::Escherichia coli heat-stable enterotoxin (ST) binds to receptors on rat intestinal cells and brush border membranes (BBM). We devised experiments to examine the reversibility of ST binding. We found that both 125I-labeled ST and native ST were spontaneously dissociable from the BBM receptor. Radiolabeled ST bound to B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.2.329-334.1987
更新日期:1987-02-01 00:00:00
abstract::Although Salmonella enterica serovar Typhimurium can undergo phase variation to alternately express two different types of flagellin subunit proteins, FljB or FliC, no biological function for this phenomenon has been described. In this investigation, we constructed phase-locked derivatives of S. enterica serovar Typhi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3021-3030.2001
更新日期:2001-05-01 00:00:00
abstract::Comparisons of the course of coccidioidomycosis in two strains of inbred mice established that BALB/c mice are significantly more susceptible to pulmonary infection with Coccidioides immitis than are DBA/2 mice. The susceptibility of BALB/c mice does not reside in their inability to mount a delayed-type hypersensitivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.1.13-17.1988
更新日期:1988-01-01 00:00:00
abstract::Although protective immunity in C57BL/6 mice induced by a single dose of the radiation-attenuated schistosome vaccine is believed to be mediated by Th1-type immune responses, we here report that in BALB/c mice protection can also depend upon signaling via the interleukin-4 (IL-4) receptor which conventionally governs ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.228-236.2001
更新日期:2001-01-01 00:00:00
abstract::Staphylococcus aureus infections are an important public health concern due to their increasing incidence and high rates of mortality. The success of S. aureus as a pathogen is highly related to its enormous capacity to evade the host immune response. The critical role of tumor necrosis factor alpha (TNF-α) in the ini...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00593-13
更新日期:2013-11-01 00:00:00
abstract::Aerobic microorganisms were isolated and identified from 9 of 10 malignant tissues aseptically obtained from surgical patients. The organisms isolated are species commonly associated with the flora of the human body. When these cancer-associated organisms were grown in Trypticase soy broth (BBL Microbiology Systems), ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1211-1215.1981
更新日期:1981-06-01 00:00:00