Abstract:
:Porphyromonas gingivalis is a major etiologic agent of periodontitis, a chronic inflammatory disease that ultimately results in the loss of the supporting tissues of the teeth. Previous work has demonstrated the usefulness of avirulent Salmonella enterica serovar Typhimurium strains as antigen delivery systems for protective antigens of pathogens that colonize or cross mucosal surfaces. In this study, we constructed and characterized a recombinant S. enterica serovar Typhimurium avirulent vaccine strain which expresses hemagglutinin A and carries no antibiotic resistance markers. HagA, a major virulence-associated surface protein, is a potentially useful immunogen that contains an antigenic epitope which, in humans, elicits an immune response that is protective against subsequent colonization by P. gingivalis. The hagA gene, including its promoter, was cloned into a balanced-lethal Salmonella vector and transferred to the vaccine strain. Heterologous expression of HagA was demonstrated in both Escherichia coli JM109 and S. enterica serovar Typhimurium vaccine strain chi4072. The HagA epitope was present in its native configuration as determined by immunochemistry and immunoelectron microscopy. Purified recombinant HagA was recognized by sera from mice immunized with the S. enterica serovar Typhimurium vaccine strain. The HagA-specific antigen of the vaccine was also found to be recognized by serum from a periodontal patient. This vaccine strain, which expresses the functional hemagglutinin protein, induces a humoral immune response against HagA and may be useful for developing a protective vaccine against periodontal diseases associated with P. gingivalis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kozarov E,Miyashita N,Burks J,Cerveny K,Brown TA,McArthur WP,Progulske-Fox Adoi
10.1128/iai.68.2.732-739.2000keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
732-9issue
2eissn
0019-9567issn
1098-5522journal_volume
68pub_type
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更新日期:1973-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1974-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1996-03-01 00:00:00
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更新日期:1978-02-01 00:00:00
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更新日期:2020-10-19 00:00:00
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journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2000-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1980-03-01 00:00:00