Abstract:
:Severe combined immunodeficient (SCID)-beige mice inoculated with the intracellular parasite Eimeria papillata produced significantly more oocysts during primary infections than inoculated immunodeficient SCID mice. Therefore, the addition of the beige mutation, which detrimentally affects neutrophil and natural killer (NK) cell functions, enhanced the parasites' ability to reproduce within the small intestine. To identify which of these two cell types is responsible for a protective immune response during primary infection, the following groups of mice were inoculated: (i) SCID mice depleted of neutrophils with antigranulocyte monoclonal antibody (RB6-8C5), (ii) C57BL/6 mice depleted of NK cells with the anti-NK-1.1 monoclonal antibody (PK136), and (iii) transgenic Tg epsilon26++ mice (T and NK cell deficient). To identify the mechanisms of immunity during primary and secondary infections, gamma interferon (IFN-gamma) knockout and perforin knockout mice were inoculated. Oocyst output was found to be significantly higher during primary infection for mice depleted of NK cells by administration of anti-NK-1.1 antibodies, for Tg epsilon26++ mice, and for IFN-gamma knockout mice. During secondary infections, only perforin knockout mice produced significantly more oocysts compared to control mice. Our observations suggest that NK cells inhibit E. papillata oocyst output during primary infection by the production of IFN-gamma and that this inhibition is independent of perforin. Immunity to reinfection does not require IFN-gamma but appears to be mediated, at least in part, by a perforin-dependent mechanism.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Schito ML,Barta JRdoi
10.1128/IAI.65.8.3165-3170.1997subject
Has Abstractpub_date
1997-08-01 00:00:00pages
3165-70issue
8eissn
0019-9567issn
1098-5522journal_volume
65pub_type
杂志文章abstract::Toxin B from Clostridium difficile is cytopathic in vitro for various types of cells, including polymorphonuclear cells, lymphocytes, and monocytes. Since intestine lamina propria is rich in macrophages, we studied the effect of toxin B on human monocytes and on human macrophages generated in vitro by long-term cultur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.1082-1090.1993
更新日期:1993-03-01 00:00:00
abstract::Gender is a significant factor in determining the susceptibility to and severity of pulmonary diseases in both humans and animals. Murine respiratory mycoplasmosis (MRM), due to Mycoplasma pulmonis infection, is an excellent animal model for evaluation of the role of various host factors on the development of acute or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2865-2871.2001
更新日期:2001-05-01 00:00:00
abstract::The outer membrane of pathogenic Leptospira species grown in culture media contains lipopolysaccharide (LPS), a porin (OmpL1), and several lipoproteins, including LipL36 and LipL41. The purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. Ha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.853-861.1999
更新日期:1999-02-01 00:00:00
abstract::Tuberculosis is rising in the developing world due to poor health care, human immunodeficiency virus type 1 infection, and the low protective efficacy of the Mycobacterium bovis BCG vaccine. A new vaccination strategy that could protect adults in the developing world from tuberculosis could have a huge impact on publi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3814-3816.2005
更新日期:2005-06-01 00:00:00
abstract::Bacteroides fragilis with enterotoxin-like activity (BFEL) was isolated from the feces of 24- to 48-h-old lambs with acute diarrheal disease on three different sheep ranches in the Northern Rocky Mountain region of the United States. The lamb intestinal loop test was used to enrich for the bacterium before its initial...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.241-244.1984
更新日期:1984-05-01 00:00:00
abstract::The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1314-1321.1992
更新日期:1992-04-01 00:00:00
abstract::Black melanin-like pigments are produced by several neurotropic fungi, including Cryptococcus neoformans. Pigment production is associated with virulence. In media containing phenolic substrates such as L-dopa, C. neoformans cells become black as a result of pigment accumulation. Pigmented and nonpigmented C. neoforma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.3131-3136.1995
更新日期:1995-08-01 00:00:00
abstract::Salmonella pathogenicity island 12 (SPI-12) of Salmonella enterica serovar Typhimurium is a 15-kb region that encompasses genes STM2230 to STM2245 and encodes a remnant phage known to contribute to bacterial virulence. In mouse infection experiments and replication assays in macrophages, we demonstrated a role for fou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00067-13
更新日期:2013-07-01 00:00:00
abstract::Infection with tissue-migrating helminths is frequently associated with intense granulocyte infiltrations. Several host-derived factors are known to mediate granulocyte recruitment to the tissues, but less attention has been paid to how parasite-derived products trigger this process. Parasite-derived chemotactic facto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.4007-4018.2001
更新日期:2001-06-01 00:00:00
abstract::CD8+ T cells are essential for protection against mycobacteria, as is clearly demonstrated by the fatal outcome of experimental infection of beta-2 microglobulin knockout mice. However, the mechanisms and antigens (Ags) leading to CD8+ T-cell activation and regulation have been poorly characterized. Here we show that,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.676-684.1997
更新日期:1997-02-01 00:00:00
abstract::The viaB locus coding for the Vi antigen of Salmonella typhi Ty2 was cloned on a 40.6-kilobase fragment into the cosmid vector pHC79. The live, oral, attenuated Vi-negative S. typhi Ty21a vaccine strain was transformed with the recombinant cosmid encoding the viaB locus. Homologous recombination of the viaB locus into...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3863-3868.1989
更新日期:1989-12-01 00:00:00
abstract::Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease in cattle. The complex, multifaceted interaction of M. avium subsp. paratuberculosis with its host includes dampening the ability of infected cells to respond to stimuli that promote M. avium subsp. paratuberculosis clearance. By dis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00785-12
更新日期:2013-01-01 00:00:00
abstract::Some species of the genus Mycoplasma code for the arginine deiminase pathway (ADI), which enables these bacteria to produce ATP from arginine by the successive reaction of three enzymes: arginine deiminase (ArcA), ornithine carbamoyltransferase (ArcB), and carbamate kinase (ArcC). It so far appears that independently ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00441-13
更新日期:2013-10-01 00:00:00
abstract::A strain of a nutritionally deficient streptococcus was shown to undergo morphological aberrations according to pyridoxal concentrations in the growth medium. Filamentous rod-shaped cells, observed by electron microscopy, predominated in the presence of decreasing concentrations. Multiple invaginations in the outer ce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.414-417.1983
更新日期:1983-10-01 00:00:00
abstract::Ambient pH signaling involves a cascade of conserved Rim or Pal products in ascomycetous yeasts or filamentous fungi, respectively. Recent evidences in the fungi Aspergillus nidulans, Saccharomyces cerevisiae, Yarrowia lipolytica, and Candida albicans suggested that components of endosomal sorting complexes required f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.7977-7987.2005
更新日期:2005-12-01 00:00:00
abstract::Vibrio cholerae is the causal organism of the diarrheal disease cholera. The rugose variant of V. cholerae is associated with the secretion of an exopolysaccharide. The rugose polysaccharide has been shown to confer increased resistance to a variety of agents, such as chlorine, bioacids, and oxidative and osmotic stre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1967-1974.2000
更新日期:2000-04-01 00:00:00
abstract::Commercial fluid used for peritoneal lavage in peritonitis and in peritoneal dialysis suppressed the activity of peripheral blood leukocytes as measured by chemiluminescence, phagocytosis, and bacterial killing. Suppression was found to be due to the low pH and high osmolality of the fluid. The pH was adjusted to noni...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.130-135.1981
更新日期:1981-07-01 00:00:00
abstract::The Yersinia enterocolitica gsrA gene is a stress protein gene which was originally identified as essential for protecting cells under both extracellular environmental stress and intracellular stress in macrophages due to phagocytosis. The gsrA gene was shown to be a member of the htrA class of genes and to possess a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2190-2196.1997
更新日期:1997-06-01 00:00:00
abstract::Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02790-14
更新日期:2015-04-01 00:00:00
abstract::Alpha-2,3-sialyltransferase (Lst) is expressed on the outer membrane of Neisseria gonorrhoeae and Neisseria meningitidis and sialylates surface lipooligosaccharide (LOS), facilitating resistance to complement-mediated killing. The enzyme is constitutively expressed from a single gene (lst) and does not undergo antigen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2637-2650.2006
更新日期:2006-05-01 00:00:00
abstract::The antigenicity of Erysipelothrix rhusiopathiae T28 (serotype 2) was investigated. Antigens were solubilized from the cell surface with detergents. By means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting it was shown that the molecular weight of the main antigenic component--a nonprot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.818-822.1986
更新日期:1986-06-01 00:00:00
abstract::The 54-kDa extracellular metalloprotease ZapA is an important virulence factor of uropathogenic Proteus mirabilis. While ZapA has the ability to degrade host immunoglobulins (Igs), the dramatic attenuation of virulence in ZapA mutants suggests that this enzyme may have a broader spectrum of activity. This hypothesis w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5159-5167.2004
更新日期:2004-09-01 00:00:00
abstract::Escherichia coli O157:H7 is an important pathogen of humans. Cattle are most frequently identified as the primary source of infection, and therefore, reduction in E. coli O157:H7 prevalence in cattle by vaccination represents an attractive strategy for reducing the incidence of human disease. H7 flagella have been imp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01452-07
更新日期:2008-06-01 00:00:00
abstract::YopE is a virulence factor that is secreted into host cells infected by Yersinia species. The YopE C-terminal domain has GTPase-activating protein (GAP) activity. The YopE N-terminal domain contains an epitope that is an immunodominant CD8(+) T cell antigen during primary infection of C57BL/6 mice with Yersinia pseudo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01687-14
更新日期:2014-07-01 00:00:00
abstract::Because of the unavailability of a suitable antigen, specific in vitro lymphocyte reactivity has not been investigated in persons who have had systemic blastomycosis. Twelve persons who had recovered from blastomycosis were evaluated, and all were found to exhibit strongly positive and apparently specific in vitro cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.485-490.1981
更新日期:1981-08-01 00:00:00
abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2182-2191.2003
更新日期:2003-04-01 00:00:00
abstract::We have previously shown that infection by Trypanosoma cruzi, a parasitic protozoan, is reduced by injection of CD40 ligand (CD40L)-transfected 3T3 fibroblasts (D. Chaussabel, F. Jacobs, J. de Jonge, M. de Veerman, Y. Carlier, K. Thielemans, M. Goldman, and B. Vray, Infect. Immun. 67:1929-1934, 1999). This prompted us...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6552-6561.2005
更新日期:2005-10-01 00:00:00
abstract::The role of macrophages in protecting mice from murine cytomegalovirus (MCMV) was studied in Swiss, CBA/J, and C57BL/6J mice. CBA/J mice were more resistant to virus than were C57BL/6J mice at all ages tested. Prior treatment of adult Swiss mice with 60 mg of silica, a dose selectively toxic to macrophages, increased ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1383-1390.1974
更新日期:1974-12-01 00:00:00
abstract::Colony variants of Neisseria gonorrhoeae differ in their interactions with eucaryotic cells. When gonococci were cultivated with HeLa cell monolayers, the opacity phenotype (Op) became increasingly dominant in the subpopulation of organisms which adhered to the HeLa cells. Once bound, Op organisms displayed very low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.154-160.1986
更新日期:1986-10-01 00:00:00
abstract::Ring-necked pheasant virus, an avian leukosis virus, when injected into 10-day old chick embryos, caused angiosarcomas in the lungs of infected chickens within a short time. Angiosarcomas appeared as localized foci of proliferating cells in the lungs as early as 2 weeks posthatch, and by 6 weeks, the lungs of the infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.310-319.1983
更新日期:1983-04-01 00:00:00