Abstract:
:The protective efficacy of antibodies to the Staphylococcus aureus type 5 capsular polysaccharide (CP5) was examined in a modified model of catheter-induced endocarditis. Rats were catheterized by surgically passing a polyethylene catheter through the right carotid artery and aortic valve into the left ventricle. The following day, the rats were injected by the intraperitoneal (i.p.) route with immunoglobulin G (IgG) purified from nonimmunized rabbits or from rabbits immunized with a conjugate vaccine composed of CP5 and CP8 linked covalently to recombinant Pseudomonas aeruginosa exotoxoid A. One day after passive immunization, the animals were challenged i.p. with one of three serotype 5 S. aureus isolates (strain Reynolds, Lowenstein, or VP) or nontypeable strain 521. Protection was evaluated by comparing quantitative cultures of blood, endocardial vegetations, and kidneys from control and immune animals. For experiments performed with S. aureus Reynolds and Lowenstein, rats given capsular antibodies (645 microg of CP5-specific IgG) showed a significantly (P < 0.05) lower prevalence of endocarditis than rats injected with nonimmune IgG. Similarly, quantitative cultures of the blood, kidneys, and aortic valve vegetations revealed that fewer S. aureus cells were recovered from rats given capsule-specific IgG than from rats administered nonimmune IgG. Rats challenged with strain VP were protected with 1.145 mg of CP5-specific IgG. Capsular antibodies did not protect against infection elicited by a nontypeable strain. These results demonstrate that capsular antibodies elicited by immunization with a polysaccharide-protein conjugate vaccine protect experimental animals against serotype 5 S. aureus infection in a modified model of endocarditis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lee JC,Park JS,Shepherd SE,Carey V,Fattom Adoi
10.1128/IAI.65.10.4146-4151.1997subject
Has Abstractpub_date
1997-10-01 00:00:00pages
4146-51issue
10eissn
0019-9567issn
1098-5522journal_volume
65pub_type
杂志文章abstract::Human lactoferrin is an iron-binding glycoprotein that is particularly prominent in exocrine secretions and leukocytes and is also found in serum, especially during inflammation. It is able to sequester iron from microbes and has immunomodulatory functions, including inhibition of both complement activation and cytoki...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3372-3381.2001
更新日期:2001-05-01 00:00:00
abstract::Chromatographic analysis, coupled to mass spectrometry with a high-resolution mass spectrometer, of materials isolated from skin lesions of patients with lepromatous leprosy allows the recognition of characteristic mycobacterial products, mycolic acids. This finding indicates that the "noncultivable" bacteria responsi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.236-239.1974
更新日期:1974-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.568-575.1999
更新日期:1999-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.810-816.1989
更新日期:1989-03-01 00:00:00
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doi:10.1128/IAI.01078-08
更新日期:2009-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1993-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00132-06
更新日期:2006-08-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2008-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.254-257.1980
更新日期:1980-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.825-827.1987
更新日期:1987-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.869-878.1979
更新日期:1979-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.567-572.1998
更新日期:1998-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.183-188.1977
更新日期:1977-10-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2002-05-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2006-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00080-20
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1998-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1113-1120.2002
更新日期:2002-03-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1983-07-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2002-07-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2009-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00