Abstract:
:Liver-stage antigen 1 (LSA-1) is a potential vaccine candidate against preerythrocytic stages of malaria. We report here the immunogenicity of linear synthetic constructs delineated as T(H)-cell determinants from the nonrepeat regions of Plasmodium falciparum LSA-1 in murine models and human subjects from areas where malaria is endemic in Rajasthan State, India. Seven peptide constructs (LS1.1 to LS1.7) corresponding to predicted T-cell sites from both the N- and C-terminal regions and peptide LS1R from a repeat region of PfLSA-1 were synthesized to analyze the cellular immune responses. These linear peptides were also tested for humoral responses in order to determine if there were any overlapping B-cell epitopes in the predicted T-cell sites. Most peptides induced cellular responses in peptide-immunized BALB/c and C57BL/6 mice as measured by proliferation and cytokine analysis. Cross-reactive T-cell recognition of P. falciparum-based peptides in Plasmodium berghei-immune animals was evaluated, but only one peptide, LS1.2 (amino acids 1742 to 1760) triggered T-cell proliferation and interleukin-2 and gamma interferon secretion in P. berghei-immune splenocytes of BALB/c and C57BL/6 mice as well as in Thamnomys gazellae (natural host of P. berghei ANKA). In an enzyme-linked immunosorbent assay with the peptides, only one peptide, LS1.1, was recognized by anti-P. berghei liver-stage serum. Three peptides (LS1. 1, LS1.2, and LS1.3) of the eight peptides tested in this study were recognized by a relatively large percentage of P. falciparum-exposed human subjects; the reactivities ranged from approximately 45% for LS1.3 to approximately 60% for LS1.1 and LS1.2. Interestingly, all of the eight putative T-cell determinants were also recognized by the sera collected from malaria patients, although the response was variable in nature. These T(H)- and B-cell epitopes may be of potential value for preerythrocytic antigen-based malaria subunit vaccine formulations.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Joshi SK,Bharadwaj A,Chatterjee S,Chauhan VSdoi
10.1128/iai.68.1.141-150.2000keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
141-50issue
1eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::Cytokine mRNA expression in biopsies of Mycobacterium ulcerans-infected human tissue was investigated using real-time PCR, and the findings were correlated with the clinical stages of disease and histopathologies. A broad range of cytokine mRNAs were detected in 16 early nodules and 28 late-stage ulcers, including tho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2917-2924.2006
更新日期:2006-05-01 00:00:00
abstract::While investigating the requirement for phagosomal alkalinization in the host defense against pulmonary aspergillosis, we observed high morbidity of p47(phox)(-/-) mice infected with pH-insensitive Aspergillus nidulans mutants despite a paucity of fungal growth. Fatal infection also resulted from a normally avirulent ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5204-5207.2005
更新日期:2005-08-01 00:00:00
abstract::Two glucosyltransferase genes from Streptococcus mutans GS-5, gtfB and gtfC, have been previously isolated and sequenced in this laboratory. In the present communication a third gtf gene, gtfD, was isolated and characterized. Isolation of the gene involved a novel procedure utilizing the integration plasmid pVA891. A ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.2079-2085.1989
更新日期:1989-07-01 00:00:00
abstract::Immunization of mice with Plasmodium berghei or Plasmodium yoelii synthetic linear peptide chimeras (LPCs) based on the circumsporozoite protein protects against experimental challenge with viable sporozoites. The immunogenicity of LPCs is significantly enhanced by spontaneous polymerization. To better understand the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00470-08
更新日期:2009-05-01 00:00:00
abstract::Early-onset periodontitis (EOP) is characterized by rapidly progressive alveolar bone loss, chemotactic defects of neutrophils, and significant familial aggregation. We found immature myeloid lineage cells, defined as promyelocytes, in the peripheral blood in patients with EOP. A hematological examination of periphera...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3906-3912.1997
更新日期:1997-09-01 00:00:00
abstract::Splenic lymphocytes from cytomegalovirus-infected mice lost their in vitro proliferative responses to cytomegalovirus antigen within 3 h after in vivo treatment with antilymphocyte globulin and prednisolone. The response was inhibited when the agents were administered separately or together, and inhibition persisted t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::A substance with potent decomplementation activity was isolated from staphylococcal culture supernatants by polyethylene glycol precipitation, DEAE-ion-exchange and Sephacryl chromatography, and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified substance exhibited all the characterist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.47-51.1985
更新日期:1985-01-01 00:00:00
abstract::In previous work, we identified xanthine oxidase (XO) as an important enzyme in the interaction between the host and enteropathogenic Escherichia coli(EPEC) and Shiga-toxigenic E. coli(STEC). Many of the biological effects of XO were due to the hydrogen peroxide produced by the enzyme. We wondered, however, if uric ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01389-15
更新日期:2016-03-24 00:00:00
abstract::Three monoclonal antibodies (MAbs), E4, L1-4, and L1-24, to the major outer membrane protein (MOMP) of Chlamydia trachomatis were identified that neutralized in vitro the infectivity of members of the B- and C-related complex as well as the mouse pneumonitis strain. MAbs L1-4, L1-24, and E4 gave a strong signal in an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4147-4153.1991
更新日期:1991-11-01 00:00:00
abstract::Conditions are described for the production of an extracellular cytotoxin or CHO cell-killing factor by Vibrio fluvialis, a recently recognized enteric pathogen. The cell-killing factor was ammonium sulfate precipitable, heat labile, and inactivated by proteases, and had an isoelectric point (estimated by sucrose dens...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.773-777.1984
更新日期:1984-12-01 00:00:00
abstract::The mechanism of activation of intestinal guanylate cyclase by Escherichia coli heat-stable enterotoxin (STa) has been studied by using isolated rat intestinal epithelial cells and purified brush border membrane (BBM) preparations. Inhibitors of prostaglandin biosynthesis, quinacrine and 5,8,11,14-eicosatetraynoic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.493-501.1984
更新日期:1984-05-01 00:00:00
abstract::The adherence of Trichomonas vaginalis NYH 286 to host cells was evaluated by using monolayer cultures of HeLa and HEp-2 epithelial cells and human fibroblast cell lines. Saturation of sites on HeLa cells was achieved, yielding a maximal T. vaginalis NYH 286-to-cell ratio of two. The ability of radiolabeled NYH 286 to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.701-708.1985
更新日期:1985-12-01 00:00:00
abstract::Mouse spleen cells which normally cannot support the in vivo replication of vesicular stomatitis virus (VSV) became susceptible to VSV infection after the intraperitoneal growth of certain syngeneic and allogeneic tumors. After 3 days' growth of P815 tumor cells in syngeneic DBA/2 mice, the viral-permissive state for ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1014-1023.1981
更新日期:1981-06-01 00:00:00
abstract::The invasion of red blood cells (RBCs) by Plasmodium falciparum is dependent on multiple molecular interactions between erythrocyte receptors and parasite ligands. Invasion studies using culture-adapted parasite strains have indicated significant receptor heterogeneity. It is not known whether this heterogeneity refle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.5886-5891.2004
更新日期:2004-10-01 00:00:00
abstract::The innate immune system recognizes pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRR) and transduces downstream signaling to activate the host defense. Here we report that in addition to direct PAMP-PRR interactions, live Candida albicans cells can release soluble factors to ac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01041-09
更新日期:2010-01-01 00:00:00
abstract::Candida albicans is a common opportunistic fungal pathogen and is the leading cause of invasive fungal diseases in immunocompromised individuals. The induction of cell-mediated immunity to C. albicans is one of the main tasks of cells of the innate immune system, and in vitro evidence suggests that integrin α(M)β₂ (CR...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00650-10
更新日期:2011-04-01 00:00:00
abstract::Entamoeba histolytica and its reptilian counterpart and encystation model Entamoeba invadens formed a polarized monopodial morphology when treated with pentoxifylline. This morphology was propelled by retrograde flow of the cell surface resulting from a cyclic sol-gel conversion of cytoplasm and a stable bleb at the l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00903-19
更新日期:2020-07-21 00:00:00
abstract::The interaction of plasma fibronectin with C1q of the complement system has been demonstrated in the past several years. In addition, the antibody-independent binding of C1q to bacteria, as well as the binding of plasma fibronectin to bacteria, is well documented. This study examines whether the binding of C1q to bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.664-669.1985
更新日期:1985-09-01 00:00:00
abstract::Several monoclonal antibodies directed against gonococcal pili have been used to investigate the potential contribution of anti-pilus antibodies to host defense against gonococcal infection. Included were two antibodies (SM1 and SM2) which reacted with conserved determinants present on pili from all strains tested and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.621-628.1985
更新日期:1985-09-01 00:00:00
abstract::Infection of C57BL/6 mice with the third-stage larvae of Brugia pahangi results in a rapid expansion of NK1.1(+) T cells in the spleen and draining lymph nodes. NK T cells produced interleukin-4 in the spleen within 24 h of infection, and these cells were CD4(-). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2215-2219.2002
更新日期:2002-04-01 00:00:00
abstract::A gene bank of group A Streptococcus strain Manfredo (M protein serotype 5) was constructed with a bacteriophage lambda vector-Escherichia coli K-12 host system and screened by immunoblotting hybrid phage plaques with antisera raised to purified pep M5 (serotype 5 M protein fragment released from the streptococcal cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.190-197.1985
更新日期:1985-04-01 00:00:00
abstract::Both dextransucrase and mutansynthetase activities have been purified from the culture fluids of Streptococcus mutans GS-5 (serotype c). Although homogeneous dextransucrase preparations normally synthesize little insoluble glucan, essentially all of the glucan synthesized by this enzyme in the presence of 1.5 M (NH4)2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.763-770.1983
更新日期:1983-11-01 00:00:00
abstract::Kinetoplastid membrane protein 11 (KMP-11) from Leishmania donovani is an abundant 11-kDa surface membrane glycoprotein. Lymph node cells from mice of six different H-2 haplotypes immunized with KMP-11 or with L. donovani promastigotes were stimulated to proliferate in vitro KMP-11. Primed purified T cells required an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4893-4899.1994
更新日期:1994-11-01 00:00:00
abstract::Previous studies have suggested that interleukin-4 (IL-4) has a protective effect in host defense to Borrelia burgdorferi infection, both in limiting the severity of arthritis and in controlling spirochete numbers in tissues, and a mapping study revealed suggestive linkage to a cluster of genes on mouse chromosome 11,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5603-5609.2000
更新日期:2000-10-01 00:00:00
abstract::A protein antigen called TR-o was isolated from supernatant of a sonically treated Reiter treponeme. The isolation procedure included anion-exchange chromatography on Whatman DE-52, hydrophobic interaction chromatography on decyl agarose, and finally gel filtration on Ac-A-22 Ultrogel. The fractionations were monitore...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.974-978.1982
更新日期:1982-03-01 00:00:00
abstract::The cell surface of Chlamydia psittaci seems important for establishing infection since (i) UV-treated elementary bodies (EB) attach to and are ingested by L cells and (ii) heat or antibody treatment decreases attachment to L cells and promotes the fusion of chlamydiae-containing phagosomes with lysosomes in macrophag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.741-751.1983
更新日期:1983-05-01 00:00:00
abstract::In mammalian hosts, Leishmania sp. parasites are obligatory intracellular organisms that invade macrophages and dendritic cells (DC), where they reside in endocytic organelles termed parasitophorous vacuoles (PV). Most of the present knowledge of the characteristics of PV harboring Leishmania sp. is derived from studi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1305-1312.2006
更新日期:2006-02-01 00:00:00
abstract::We examined the way the major outer membrane protein OmpA of Salmonella enterica serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs) against this protein. Interaction between OmpA and these MAbs is competitively inhibited with several-hundredfold dilution...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3937-3946.2003
更新日期:2003-07-01 00:00:00
abstract::Incubation of large granular lymphocytes (LGL) with glutaraldehyde-fixed bacteria stimulated in the supernatant the production of interferon (IFN), which proved to be mainly IFN-gamma. Even though IFN-gamma was produced upon exposure of LGL to bacteria, anti-IFN-gamma antibodies failed to interfere with induction of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2767-2773.1991
更新日期:1991-08-01 00:00:00
abstract::Enterococcus faecalis strains are resident intestinal bacteria associated with invasive infections, inflammatory bowel diseases, and colon cancer. Although factors promoting E. faecalis colonization of intestines are not fully known, one implicated pathway is a phosphotransferase system (PTS) in E. faecalis strain OG1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-19
更新日期:2019-06-20 00:00:00