Abstract:
:Nine goats were injected transthoracically with Pasteurella haemolytica A1 to determine if an extracellular bacterial enzyme, neuraminidase, was produced in vivo during infection with this organism. The principal group of goats (n = 9) each received 1 ml of 7.25 x 10(5) live P. haemolytica A1 cells in polyacrylate beads transthoracically in the left lung on days 0 and 21. Six goats were used as negative controls and received 0.3 g of polyacrylate beads subcutaneously in the right flank on days 0 and 21. Serum was obtained from all animals on days -4, 3, 7, 14, 21, 24, and 32. Preimmune serum from all animals showed no detectable antibody to P. haemolytica A1 neuraminidase in an enzyme neutralization assay. None of the sera from the negative control animals possessed a significant antibody concentration in response to the P. haemolytica A1 neuraminidase. On day 32, serum samples from the nine infected animals possessed enzyme neutralizing activity that ranged from 62% to 100%. Anti-neuraminidase antibody could be detected as early as day 14 by the enzyme neutralization assay. These data demonstrate that the enzyme neuraminidase is produced in vivo during an active P. haemolytica A1 lobar infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Straus DC,Purdy CWdoi
10.1128/IAI.62.10.4675-4678.1994subject
Has Abstractpub_date
1994-10-01 00:00:00pages
4675-8issue
10eissn
0019-9567issn
1098-5522journal_volume
62pub_type
杂志文章abstract::Stools from a patient with antibiotic-associated colitis and cecal contents from a hamster with clindamycin-induced enterocolitis were compared in a cytotoxicity assay to determine common properties. Both specimens produced actinomorphic changes in human amnion cells at 10(-7) dilutions. The toxin was acid labile, hea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.526-529.1978
更新日期:1978-05-01 00:00:00
abstract::Secretion of immunoglobulin A1 (IgA1) proteases is a characteristic of Haemophilus influenzae and several other bacterial pathogens causing infectious diseases, including meningitis. Indirect evidence suggests that the proteases are important virulence factors. In this study, we cloned the iga gene encoding immunoglob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3097-3105.1989
更新日期:1989-10-01 00:00:00
abstract::Proliferation of Bordetella pertussis in the lungs of infant mice challenged by the intranasal route was examined. The bacteria rapidly proliferated in the lungs of mice challenged with a sublethal dose of a wild-type strain (BP338) or a filamentous hemagglutinin mutant (BPM409) from 500 at day 0 to 10(7) at day 15. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3445-3447.1990
更新日期:1990-10-01 00:00:00
abstract::Cells infected by Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, display unusual intracellular morphological changes characterized by dilatation of the membranes of the endoplasmic reticulum and outer nuclear envelope. These changes are consistent with those that might be expected to occur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3110-3115.1988
更新日期:1988-12-01 00:00:00
abstract::Routine culture of endothelial cells currently includes the use of heparin, which significantly reduces cell doubling time and increases cell population size. Heparin protects cultured arterial endothelial cells from damage by toxic oxygen metabolites produced by the action of xanthine and xanthine oxidase. Because of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4505-4510.1991
更新日期:1991-12-01 00:00:00
abstract::Studies conducted with F1 and F2 progeny of crosses between strains of inbred mice that differ greatly in their capacity to make an antibody response to type III pneumococcal polysaccharide, dextran B-1355, and lipopolysaccharide from Escherichia coli 0113 have shown that multiple genes influence the magnitude of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.56-61.1984
更新日期:1984-07-01 00:00:00
abstract::Cells in the Brucella spp. are intracellular pathogens that survive and replicate within host monocytes. Brucella maintains persistent infections in animals despite the production of high levels of anti-Brucella-specific antibodies. To determine the effect of antibody opsonization on the ability of Brucella to establi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3702-3713.2005
更新日期:2005-06-01 00:00:00
abstract::The initial encounter of Leishmania cells and cells from the immune system is fundamentally important in the outcome of infection and determines disease development or resistance. We evaluated the anti-Leishmania amazonensis response of naive volunteers by using an in vitro priming (IVP) system and comparing the respo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7453-7460.2001
更新日期:2001-12-01 00:00:00
abstract::B cell activating factor of the tumor necrosis factor family (BAFF) is an essential survival factor for B cells and has been shown to regulate T cell-independent (TI) IgM production. During Ehrlichia muris infection, TI IgM secretion in the spleen was BAFF dependent, and antibody-mediated BAFF neutralization led to an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00998-13
更新日期:2013-12-01 00:00:00
abstract::The infiltration of neutrophils which phagocytose and kill microorganisms is an important defense mechanism against infections of the airways. Bordetella pertussis is a human respiratory pathogen which colonizes ciliated epithelium, causing whooping cough. We have investigated the effects of the peptidoglycan fragment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.639-643.1994
更新日期:1994-02-01 00:00:00
abstract::Schistosoma mansoni eggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00647-09
更新日期:2010-02-01 00:00:00
abstract::Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2416-2419.1987
更新日期:1987-10-01 00:00:00
abstract::Oropharyngeal candidiasis (OPC [thrush]) is an opportunistic infection caused by the commensal fungus Candida albicans. OPC is common in individuals with HIV/AIDS, infants, patients on chemotherapy, and individuals with congenital immune defects. Immunity to OPC is strongly dependent on the interleukin-23 (IL-23)/IL-1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01335-13
更新日期:2014-03-01 00:00:00
abstract::The purpose of these experiments was to study the early response of mouse foot pads to Mycobacterium leprae. To accomplish this, mice were inoculated in both foot pads with large and small numbers of organisms. The animals were sacrificed at intervals from 2 hr to 27 days after inoculation. The microscopical results, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.1.76-85.1973
更新日期:1973-01-01 00:00:00
abstract::Chronic pulmonary infection with Pseudomonas aeruginosa is a feature of cystic fibrosis (CF) and other chronic lung diseases. Cytokines of the interleukin-17 (IL-17) family have been proposed as important in the host response to P. aeruginosa infection through their role in augmenting antibacterial immune responses, a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00717-16
更新日期:2016-11-18 00:00:00
abstract::Mice immunized with infections of 500, 5,000, or 20,000 oocysts of E. falciformis var. pragensis were reinfected with 20,000 and 100,000 oocysts at 20 and 38 days, respectively, after the initial infection. After the first challenge infection, none of the immunized mice showed clinical signs of coccidiosis; a few mice...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.1.108-114.1979
更新日期:1979-01-01 00:00:00
abstract::Cyclic dimeric GMP (c-di-GMP), a bacterial second messenger, is known to regulate bacterial biofilm and sessility. Replication of an obligatory intracellular pathogen, Ehrlichia chaffeensis, is characterized by formation of bacterial aggregates called morulae inside membrane-bound inclusions. When E. chaffeensis matur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05320-11
更新日期:2011-10-01 00:00:00
abstract::In order to evaluate during experimental Trypanosoma brucei infections the potential role of tumor necrosis factor alpha (TNF-alpha) in the host-parasite interrelationship, C57BL/6 TNF-alpha knockout mice (TNF-alpha-/-) as well as C57BL/6 wild-type mice were infected with pleomorphic T. brucei AnTat 1.1 E parasites. I...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.3128-3132.1999
更新日期:1999-06-01 00:00:00
abstract::Scrub typhus immunogens were prepared by exposing infected yolk sac suspensions of Rickettsia tsutsugamushi to various doses of gamma radiation. Mouse lethality was abolished at doses greater than 200 krads, whereas immunogenicity of the suspensions, as shown by mouse protection tests, was diminished relatively little...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.124-131.1977
更新日期:1977-01-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme disease, has a complex genome consisting of a linear chromosome and up to 21 linear and circular plasmids. These plasmids encode numerous proteins critical to the spirochete's infectious cycle and many hypothetical proteins whose functions and requirements are unknown....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-09
更新日期:2010-06-01 00:00:00
abstract::Virulence of the fungal pathogen Aspergillus fumigatus is in part based on the saprophytic lifestyle that this mold has evolved. A crucial function for saprophytism resides in secreted proteases that allow assimilation of proteinaceous substrates. The impact of extracellular proteolytic activities on the pathogenesis ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00425-09
更新日期:2009-09-01 00:00:00
abstract::Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella abortus (BCSP31). Recombinant S. choleraesuis chi 3781 stably maintained the pBA31-R7 plasmid and continued to express t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.610-618.1993
更新日期:1993-02-01 00:00:00
abstract::Cell-mediated immunity plays an important role in immunity to the pathogenic fungus Cryptococcus neoformans. However, the antigen specificity of the T-cell response to C. neoformans remains largely unknown. In this study, we used two approaches to determine the antigen specificity of the T-cell response to C. neoforma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-06
更新日期:2006-08-01 00:00:00
abstract::Fusobacterium nucleatum is closely associated with human periodontal diseases and may also be a causative agent in other infections, such as pericarditis, septic arthritis, and abscesses of tonsils and liver. Initiation and outcome of infective diseases depend critically on the host cell signaling system altered by th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.1171-1179.2005
更新日期:2005-02-01 00:00:00
abstract::Administration of free muramyl tripeptide phosphatidylethanolamide (MTPPE) or liposome-encapsulated MTPPE (LE-MTPPE) in a twofold-lower dose at 24 h before bacterial inoculation resulted in clearance of intravenously inoculated Klebsiella pneumoniae by tissue macrophages, whereas in control mice, bacteria were not eff...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4891-4897.1992
更新日期:1992-11-01 00:00:00
abstract::The mig gene of Streptococcus dysgalactiae, a major bovine mastitis pathogen, encodes two plasma protein-binding receptors, alpha2-macroglobulin (alpha2-M) and immunoglobulin G (IgG). In this study, the mig gene from one S. dysgalactiae isolate was cloned and expressed in Escherichia coli. The IgG receptor region enco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.10.6030-6037.2001
更新日期:2001-10-01 00:00:00
abstract::A high-molecular-weight, immunogenic form of the lipopolysaccharide O side chain of Pseudomonas aeruginosa Fisher immunotype 4 (type 4, International Antigenic Typing System 1, Lanyi O:6) was isolated and characterized. Analysis by nuclear magnetic resonance spectroscopy confirmed the structural similarity of this hig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.426-431.1989
更新日期:1989-02-01 00:00:00
abstract::Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacterial proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tube...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5628-5635.2005
更新日期:2005-09-01 00:00:00
abstract::PspA is an antigenically variable surface protein of Streptococcus pneumoniae that appears to be essential for full pneumococcal virulence. In addition, monoclonal antibodies to PspA protect mice against infection with specific strains of pneumococci virulent for mice. In this study, we have isolated the 43-kDa N-term...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1285-1289.1991
更新日期:1991-04-01 00:00:00
abstract::A murine model system was established to study immune responses to the Pnu-Imune vaccine, which is made up of 23 different pneumococcal capsular polysaccharides. In this animal model, antibody-forming cell responses to 21 of 23 individual polysaccharides in the vaccine were detected. The Pnu-Imune vaccine elicited goo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2329-2336.1992
更新日期:1992-06-01 00:00:00