Abstract:
:Recent reports have demonstrated that Bordetella pertussis has invasive behavior in vivo and in vitro. In this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants deficient in specific virulence factors to enter and survive intracellularly in human macrophages in vitro. Uptake of virulent B. pertussis was dose dependent and occurred in the absence of serum or specific antibody, with entry occurring via a microfilament-dependent phagocytic process. The virulent wild-type parental strain was internalized and persisted intracellularly over the 3 days of experiments, as determined by transmission electron microscopy and by recovery of viable plate counts. This is the first report of long-term survival of B. pertussis in human macrophages. Avirulent mutants entered macrophages, but at only an average of 1.5% of virulent parental levels, and did not survive intracellularly. Mutants which did not express adenylate cyclase toxin, filamentous hemagglutinin, or pertussis toxin had decreased abilities to enter and to survive inside macrophages. The results suggest that the internalization process, as well as intracellular survival, is virulence dependent and that mutations which inactivate expression of virulence factors may affect both. The ability of B. pertussis to enter and persist inside macrophages may be important not only for survival of the bacteria but also in the pathogenesis of whooping cough.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Friedman RL,Nordensson K,Wilson L,Akporiaye ET,Yocum DEdoi
10.1128/IAI.60.11.4578-4585.1992keywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
4578-85issue
11eissn
0019-9567issn
1098-5522journal_volume
60pub_type
杂志文章abstract::The antigens of Haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a protein A-peroxidase conjugate. The same two 76K and 40K antigens were predominant in whole-bacterium preparations and in outer-membrane-enric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1381-1386.1987
更新日期:1987-06-01 00:00:00
abstract::Susceptibility to the lethal toxicity of endotoxin in mice pretreated with emulsified trehalose-6,6'-dimycolate was enhanced by (i) increasing the oil concentration while keeping the Tween concentration constant; and (ii) decreasing the Tween concentration, while keeping the oil concentration constant. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.571-572.1979
更新日期:1979-05-01 00:00:00
abstract::Three dominant proteins (41, 48, and 49 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in purified preparations of the extracellular aspartyl proteinase (AP) of Candida albicans. All three proteins bound to the specific carboxyl proteinase ligand, pepstatin A, and were assoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2030-2036.1993
更新日期:1993-05-01 00:00:00
abstract::Poly-N-acetyl-glucosamine (PNAG) is a staphylococcal surface polysaccharide influencing biofilm formation that is also under investigation for its vaccine potential. Antibodies that bind to PNAG with either low (<15%) or high (>90%) levels of acetate are superior at opsonic and protective activity compared with antibo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00078-07
更新日期:2007-07-01 00:00:00
abstract::The cytoskeletal lesions associated with enteropathogenic Escherichia coli adhering to cultured HeLa epithelial cells were examined by immunofluorescence microscopy. The microfilament-associated proteins actin, alpha-actinin, talin, and ezrin were localized with adherent enteropathogenic E. coli, whereas tropomyosin, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2541-2543.1992
更新日期:1992-06-01 00:00:00
abstract::The lungs of conventional rats with chronic pneumonia contained Streptobacillus moniliformis and Mycoplasma pulmonis. These organisms singly and in combination failed to produce lung disease when inoculated into specific pathogen-free rats. On the other hand, diseased lung homogenate not containing cultivable organism...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.1.83-91.1972
更新日期:1972-07-01 00:00:00
abstract::The effects of infection with Rowson-Parr virus (RPV), an associated virus present in Friend virus (FV) preparations, on the splenic plaque-forming cell response to sheep red cells in adult mice and on the plaque-forming cell response of peritoneal cells in vitro have been investigated. The results support the hypothe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.2.160-166.1973
更新日期:1973-02-01 00:00:00
abstract::Brucella organisms are intracellular stealth pathogens of animals and humans. The bacteria overcome the assault of innate immunity at early stages of an infection. Removal of polymorphonuclear neutrophils (PMNs) at the onset of adaptive immunity against Brucella abortus favored bacterial elimination in mice. This was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00118-19
更新日期:2019-04-23 00:00:00
abstract::We have previously described (L. M. Vaughan, P. R. Smith, and T. J. Foster, Infect. Immun. 61:2172-2181, 1993) the construction of a kanamycin-resistant aromatic-dependent mutant of Aeromonas salmonicida, the causative agent of furunculosis, and its use as a live vaccine. Here we describe the construction of an unmark...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3863-3869.1996
更新日期:1996-09-01 00:00:00
abstract::Bacterial recognition of host sialic acid-containing receptors plays an important role in microbial colonization of the human oral cavity. The sialic acid-binding adhesin of Streptococcus gordonii DL1 was previously associated with the hsa gene encoding a 203-kDa protein. The predicted protein sequence consists of an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3876-3882.2004
更新日期:2004-07-01 00:00:00
abstract::A 75-kilodalton major protein (75K protein) was purified to homogeneity from the cell lysate fraction and the envelope of Bacteroides gingivalis 381. The 75K protein was originally present in the outer membrane or the outermost part of this organism as a large, stable complex with an apparent molecular weight of about...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3646-3652.1989
更新日期:1989-11-01 00:00:00
abstract::An immunological assay utilizing double-diffusion principles was developed for identification of Pseudomonas aeruginosa mutants deficient in toxin A. Mutations were chemically induced, and mutants were isolated from P. aeruginosa strain PA103. Quantitative assays, both enzymatic and immunological, indicated that five ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Infantile gastroenteritis virus (orbi-group) recovered from stools of infants with acute nonbacterial gastroenteritis was administered per os to germfree and conventional piglets. Virus was found subsequently in stools and in the mucosal epithelial cells of the small intestine of these animals. Some animals developed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.6.1276-1280.1975
更新日期:1975-12-01 00:00:00
abstract::Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8+ T cells are critical for control of T. cruzi infection, and CD8+ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00275-10
更新日期:2010-07-01 00:00:00
abstract::The mechanism of complement-mediated killing of seven serum-sensitive Pseudomonas aeruginosa strains was examined. All seven strains were sensitive to the bactericidal activity of 20% pooled normal human serum (PNHS) containing magnesium EGTA, which blocks the classical complement pathway (CCP), or 20% PNHS preheated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.3.632-639.1988
更新日期:1988-03-01 00:00:00
abstract::Current evidence indicates that Mycobacterium avium infection in patients with AIDS is acquired mostly through the gastrointestinal (GI) tract and that M. avium binds to and invades GI mucosal cells in vitro. Since M. avium is exposed to specific environmental conditions in the GI tract such as changes in pH, low oxyg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3768-3773.1997
更新日期:1997-09-01 00:00:00
abstract::Antibiotic-resistant Klebsiella pneumoniae isolates constitute a great clinical challenge. One important resistance mechanism in K. pneumoniae is the metallo-β-lactamases (MBLs), which require zinc for their function. Thus, zinc chelation could be a strategy to resensitize K. pneumoniae to β-lactams. However, the pote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00756-19
更新日期:2019-12-17 00:00:00
abstract::A previously undescribed, immunoreactive, membranous spherule outer wall (SOW) fraction produced by Coccidioides immitis (strains 634 and 735) grown in culture was isolated. Both this fraction and intact spherules were reactive with sera from coccidioidomycosis patients, as demonstrated by immunofluorescence microscop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2686-2694.1988
更新日期:1988-10-01 00:00:00
abstract::Complement-fixing antibodies were first detected in mice 7 days after intravenous injection with Mycoplasma arthritidis. Peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. The metabolism-inhibiting antibody response was minimal. On fractionation of mouse sera, only 7S antibod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.4.431-440.1971
更新日期:1971-10-01 00:00:00
abstract::Memory CD8+ T cells are essential for protective immunity against many intracellular pathogens; therefore, stimulation of this population of cells is an important goal of vaccination. We have previously shown that a detoxified derivative of Bacillus anthracis anthrax lethal toxin (LT) can deliver heterologous CD8+ T-c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01208-07
更新日期:2008-03-01 00:00:00
abstract::Nonspecific interactions related to physicochemical properties of bacterial cell surfaces, such as hydrophobicity and electrostatic charge, are known to have important roles in bacterium-host cell encounters. Streptococcus pneumoniae (pneumococcus) expresses multiple, surface-exposed, choline-binding proteins (CBPs) w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.412-415.2002
更新日期:2002-01-01 00:00:00
abstract::This longitudinal prospective study shows that antibodies to the N-terminal block 2 region of the Plasmodium falciparum merozoite surface protein 1 (MSP-1) are associated with protection against clinical malaria in an area of stable but seasonal malaria transmission of Ghana. Antibodies to the block 2 region of MSP-1 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6492-6502.2004
更新日期:2004-11-01 00:00:00
abstract::Murine cerebral malaria (CM) induced by Plasmodium berghei ANKA kills susceptible mice within 24 to 48 h of onset of symptoms and is characterized by the production of inflammatory cytokines in the brain. C57BL/6J mice are sensitive to lethal CM, while A/J mice are resistant. These strains of mice were immunized with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.5972-5979.1998
更新日期:1998-12-01 00:00:00
abstract::Strain TG is a plasmidless exfoliative toxin (ET) producer. Strain ER201 contains a plasmid, whose loss had no apparent effect on ET production. The plasmid of ER201 was almost identical in size to the plasmid from strain UT0007, which carries an ET determinant. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.5.1259-1260.1976
更新日期:1976-11-01 00:00:00
abstract::Bacterial lipopolysaccharide (LPS) is a potent stimulus of cells, yet a target protein for LPS has not been defined. We used two approaches to define LPS-binding sites on cell surfaces: one assay measured binding of LPS-coated erythrocytes (ELPS) to cultured human cells, and a second measured binding of a radiolabeled...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.12.4069-4075.1990
更新日期:1990-12-01 00:00:00
abstract::The Duffy binding protein (DBP) is a vital ligand for Plasmodium vivax blood-stage merozoite invasion, making the molecule an attractive vaccine candidate against vivax malaria. Similar to other blood-stage vaccine candidates, DBP allelic variation eliciting a strain-specific immunity may be a major challenge for deve...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05924-11
更新日期:2012-03-01 00:00:00
abstract::Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3110-3119.2001
更新日期:2001-05-01 00:00:00
abstract::Oophorectomized, estrogen-treated rats were immunized by the intravaginal or intranasal route with a mannoprotein extract (MP) or secreted aspartyl proteinases (Sap) of Candida albicans, with or without cholera toxin as a mucosal adjuvant. Both routes of immunization were equally effective in (i) inducing anti-MP and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2725-2729.2002
更新日期:2002-05-01 00:00:00
abstract::Urinary tract infection (UTI) is the second most common infection in humans, making it a global health priority. Nearly half of all women will experience a symptomatic UTI, with uropathogenic Escherichia coli (UPEC) being the major causative agent of the infection. Although there has been extensive research on UPEC vi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-17
更新日期:2018-02-20 00:00:00
abstract::Streptococcal pyrogenic exotoxin B (SpeB), a conserved cysteine protease expressed by virtually all Streptococcus pyogenes strains, has recently been shown to be an important virulence factor (S. Lukomski, S. Sreevatsan, C. Amberg, W. Reichardt, M. Woischnik, A. Podbielski, and J. M. Musser, J. Clin. Invest. 99:2574-2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.771-776.1998
更新日期:1998-02-01 00:00:00