Abstract:
:We previously demonstrated the suppression of cell-mediated immunity to Listeria monocytogenes by Pseudomonas aeruginosa-induced, macrophage-like cells. The present study was undertaken to evaluate the mechanism for this suppression. P. aeruginosa supernatant was shown to activate macrophages by the criteria of increased bactericidal capacities and increased attachment to glass surfaces. Acquired cellular resistance to L. monocytogenes could also be inhibited by macrophages from L. monocytogenes-pretreated mice. The depression of acquired immunity by P. aeruginosa- or L. monocytogenes-activated macrophages did not appear to be due to a reduction of antigenic stimulus after nonspecific macrophage activation. In contrast, our findings suggest that suppression is mediated by activated macrophages through a prostaglandin-dependent mechanism. In vivo administration of aspirin blocked the immunosuppressive effect of P. aeruginosa- or L. monocytogenes-activated cells. Moreover, the suppressive activity of supernatants of macrophages from Listeria-infected mice was reversed when indomethacin was present during supernatant generation. Finally, prostaglandin E1 treatment in vivo profoundly inhibited the induction of cell-mediated immunity to L. monocytogenes. The possible role and mechanism of prostaglandin in suppressing cellular immunity to intracellular bacteria are discussed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Petit JC,Richard G,Burghoffer B,Daguet GLdoi
10.1128/IAI.49.2.383-388.1985subject
Has Abstractpub_date
1985-08-01 00:00:00pages
383-8issue
2eissn
0019-9567issn
1098-5522journal_volume
49pub_type
杂志文章abstract::The viaB locus coding for the Vi antigen of Salmonella typhi Ty2 was cloned on a 40.6-kilobase fragment into the cosmid vector pHC79. The live, oral, attenuated Vi-negative S. typhi Ty21a vaccine strain was transformed with the recombinant cosmid encoding the viaB locus. Homologous recombination of the viaB locus into...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3863-3868.1989
更新日期:1989-12-01 00:00:00
abstract::Helicobacter pylori infection is thought to be involved in the development of several gastric diseases. Two H. pylori virulence factors (vacuolating cytotoxin A and cytotoxin-associated gene A) reportedly interact with lipid rafts in gastric epithelial cells. The role of Toll-like receptor (TLR)-mediated signaling in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05856-11
更新日期:2012-05-01 00:00:00
abstract::We tested the hypothesis that the intensity of specific antichlamydial T cell-mediated immunity in the genital tract of female guinea pigs infected intravaginally with the chlamydial agent of guinea pig inclusion conjunctivitis would determine the resistance or susceptibility to reinfection after a primary chlamydial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.4.1346-1351.1991
更新日期:1991-04-01 00:00:00
abstract::After intranasal inoculation, Brucella melitensis chronically infects the mononuclear phagocyte system in BALB/c mice, but it causes no apparent illness. Adaptive immunity, which can be transferred by either T cells or antibody from immune to naive animals, confers resistance to challenge infection. The role of innate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5314-5320.2000
更新日期:2000-09-01 00:00:00
abstract::Analyses of chemical composition in whole cells of Rickettsia tsutsugamushi were performed and compared with those of the other rickettsiae and gram-negative bacteria. The results indicated that R. tsutsugamushi does not contain detectable amounts of 3-deoxy-D-mannooctulosonic acid, heptose, muramic acid, or glucosami...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2290-2292.1987
更新日期:1987-09-01 00:00:00
abstract::Lipopolysaccharide (LPS) is one of the most potent trigger substances for monocytes and macrophages causing secretion of tumor necrosis factor alpha (TNF-alpha) and other inflammatory mediators. The nature of the nuclear factors involved in human TNF-alpha gene regulation is still unknown. Nuclear factor kappa B (NF-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1529-1534.1995
更新日期:1995-04-01 00:00:00
abstract::The rfb locus, determining biosynthesis of O8-specific lipopolysaccharide side chains, was transferred to a rough mutant of Escherichia coli; recombinants producing a complete lipopolysaccharide were more resistant to the complement-mediated bactericidal action of human serum than the rough recipient. Inheritance of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.29.1.278-280.1980
更新日期:1980-07-01 00:00:00
abstract::The formation of water-insoluble glucan by extracellular glucosyltransferase from Streptococcus mutans 6715 found to be greatly stimulated by various mono- or divalent cations. An enzyme preparation, obtained by ethanol fractionation, was able to catalyze the formation of water-insoluble glucan from sucrose in the pre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.23.3.564-570.1979
更新日期:1979-03-01 00:00:00
abstract::Mortality associated with acute lung injury (ALI) induced by lipopolysaccharide (LPS) remains high in humans, warranting improved treatment and prevention strategies. ALI is characterized by the expression of proinflammatory mediators and extensive neutrophil influx into the lung, followed by severe lung damage. Under...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7247-7256.2004
更新日期:2004-12-01 00:00:00
abstract::The possibility that soluble products of Schistosoma mansoni eggs might participate in the pathogenesis of hepatic fibrosis in schistosomiasis was investigated. Both crude saline extracts of eggs (soluble egg antigen [SEA]) and a partially purified SEA fraction contained activity which stimulated guinea pig and human ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.1.103-108.1982
更新日期:1982-10-01 00:00:00
abstract::Vibrio cholerae O1 has two biotypes, El Tor and Classical, and the latter is now presumed to be extinct in nature. Under carbohydrate-rich growth conditions, El Tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-BD), which prevents accumulation of organic acids from mixed acid ferment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01237-10
更新日期:2011-08-01 00:00:00
abstract::As reported elsewhere, an enterotoxigenic strain of Escherichia coli serotype O25:K98:NM was epidemiologically incriminated as the etiological agent in a shipboard outbreak of diarrheal illness. This enterotoxigenic E. coli strain and possibly other enteric isolates were found to produce heat-labile toxin and not heat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.793-797.1979
更新日期:1979-06-01 00:00:00
abstract::Helicobacter mustelae is a urease-rich bacterium associated with gastritis in ferrets. The ureases of H. mustelae and Helicobacter pylori, a bacterium implicated in human gastritis, share many characteristics. Helicobacter sp. ureases appear to be unique among bacterial enzymes in exhibiting submillimolar Km values an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.3343-3345.1991
更新日期:1991-09-01 00:00:00
abstract::Proteolysis is a common microbial virulence mechanism that enables the destruction of host tissue and evasion from host defense mechanisms. Antimicrobial peptides, also known as host defense peptides, are effector molecules of the innate immunity that demonstrate a broad range of antimicrobial and immunoregulatory act...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00648-09
更新日期:2009-12-01 00:00:00
abstract::Escherichia coli strains expressing the K1 capsule are a major cause of sepsis and meningitis in human neonates. The development of these diseases is dependent on the expression of a range of virulence factors, many of which remain uncharacterized. Here, we show that all but 1 of 34 E. coli K1 neonatal isolates carrie...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00716-15
更新日期:2015-09-01 00:00:00
abstract::We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flage...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::Mucosal exposure to Escherichia coli elicits an inflammatory response in the urinary tract. Interleukin-6 (IL-6) is secreted into the urine, and polymorphonuclear leukocytes (PMNLs) are recruited to the site of infection. This study analyzed the ability of mucosally administered bacterial components to activate IL-6 a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.12.4357-4362.1991
更新日期:1991-12-01 00:00:00
abstract::Cryptococcus neoformans is a facultative intracellular opportunistic pathogen and the leading cause of fungal meningitis in humans. In the absence of a protective cellular immune response, the inhalation of C. neoformans cells or spores results in pulmonary infection. C. neoformans cells produce a polysaccharide capsu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00088-09
更新日期:2009-07-01 00:00:00
abstract::Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.751-755.1988
更新日期:1988-04-01 00:00:00
abstract::A lectin with strong hemagglutinating activity toward erythrocytes of several animal species was isolated from an 18-h culture supernatant of a diarrheagenic strain, V2, of non-O1 Vibrio cholerae. The hemagglutinin (HA) was purified free of lipopolysaccharide by salt fractionation followed by gel filtration, hydrophob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3698-3705.1990
更新日期:1990-11-01 00:00:00
abstract::Experimental Helicobacter pylori infection was studied in Mongolian gerbils with fresh human isolates that carry or do not carry cagA (cagA-positive or cagA-negative, respectively), multiply passaged laboratory strains, wild-type strain G1.1, or isogenic ureA, cagA, or vacA mutants of G1.1. Animals were sacrificed 1 t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.4856-4866.1998
更新日期:1998-10-01 00:00:00
abstract::Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella abortus (BCSP31). Recombinant S. choleraesuis chi 3781 stably maintained the pBA31-R7 plasmid and continued to express t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.610-618.1993
更新日期:1993-02-01 00:00:00
abstract::An attenuated Salmonella typhi Ty21a vaccine was administered to 18 infants and toddlers (less than or equal to 24 months old) to determine its safety and immunogenicity. The vaccination (10(9) CFU per dose, three doses) was well tolerated. However, after the vaccination there was no evidence of a humoral or cellular ...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.59.11.4291-4293.1991
更新日期:1991-11-01 00:00:00
abstract::Nitric oxide (NO) produced by inducible NO synthase (iNOS) mediates hypotension in endotoxemia. In this study, NO induction by a toxin-producing Streptococcus pyogenes isolate, H250, and by recombinant streptococcal pyrogenic exotoxin A (rSPEA) has been examined, both in vitro and in vivo. Streptococcal supernatants, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1767-1772.1997
更新日期:1997-05-01 00:00:00
abstract::The type III capsular polysaccharide of group B streptococci (GBS) consists of a linear backbone with short side chains ending in residues of N-acetylneuraminic acid, or sialic acid. The presence of sialic acid on the surface of the organism inhibits activation of the alternative pathway of complement and is thought t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.392-400.1992
更新日期:1992-02-01 00:00:00
abstract::Strain superinfection occurs when a second pathogen strain infects a host already carrying a primary strain. Anaplasma marginale superinfection occurs when the second strain carries a variant repertoire different from that of the primary strain, and the epidemiologic consequences depend on the relative efficiencies of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01518-08
更新日期:2009-04-01 00:00:00
abstract::Strains of Bacteroides fragilis associated with diarrhea in children (termed enterotoxigenic B. fragilis, or ETBF) produce a heat-labile ca. 20-kDa protein toxin (BFT). The purpose of this study was to examine the activity of BFT on polarized monolayers of human intestinal epithelial cells (T84 cells). In Ussing chamb...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3561-3570.1997
更新日期:1997-09-01 00:00:00
abstract::Salmonella infections continue to cause substantial morbidity and mortality throughout the world. However, recent discoveries and new paradigms promise to lead to novel strategies to diagnose, treat, and prevent Salmonella infections. This review provides an update of the Salmonella field based on oral presentations g...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00096-10
更新日期:2010-06-01 00:00:00
abstract::We wished to find a simple, biologically relevant method to evaluate the virulence of dengue viruses for human beings. Since cells of mononuclear phagocyte lineage may be important sites of dengue infection in primates, we evaluated the permissiveness of these cells to dengue virus as a correlate of virus virulence. T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.102-106.1981
更新日期:1981-01-01 00:00:00
abstract::Immunization with live Plasmodium sporozoites under chloroquine prophylaxis (Spz plus CQ) induces sterile immunity against sporozoite challenge in rodents and, more importantly, in humans. Full protection is obtained with substantially fewer parasites than with the classic immunization with radiation-attenuated sporoz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01415-09
更新日期:2010-05-01 00:00:00