Abstract:
:A lectin with strong hemagglutinating activity toward erythrocytes of several animal species was isolated from an 18-h culture supernatant of a diarrheagenic strain, V2, of non-O1 Vibrio cholerae. The hemagglutinin (HA) was purified free of lipopolysaccharide by salt fractionation followed by gel filtration, hydrophobic interaction chromatography, and, finally, gel filtration in the presence of urea and deoxycholate. The purification procedure resulted in an HA preparation with 80-fold enhancement of specific activity. The HA consisted of noncovalently bound subunits of Mr 62,000 and behaved essentially as a single component with pI 6.0. Nonpolar and acidic amino acids contributed 46 and 24%, respectively, to the total amino acid residues. Electron micrographs of the HA showed it to consist of large, nonstoichiometric aggregates' of disklike molecules of 10-nm diameter. Inhibition of the HA by the glycoproteins fetuin, asialofetuin, and mucin, but not by ovalbumin and simple sugars, suggested the specific requirement of complex carbohydrates for binding. Rabbit antisera to the purified HA inhibited the hemagglutinating activities of the crude cell-free HA preparations, but not cell-associated HA activities of the parent (V2) or of other O1 and non-O1 V. cholerae strains. This suggested that the released and cell-associated HA activities were mediated by antigenically distinct components. Immunoblotting experiments showed that the antisera recognized a polypeptide component of Mr 62,000 in the cell envelope preparations of the parent and several other V. cholerae O1 and non-O1 strains. These data suggested that the HA was a nonfimbrial lectin of somatic origin with no protease activity and was apparently distinct from V. cholerae HAs described so far.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Banerjee KK,Ghosh AN,Dutta-Roy K,Pal SC,Ghose ACdoi
10.1128/IAI.58.11.3698-3705.1990subject
Has Abstractpub_date
1990-11-01 00:00:00pages
3698-705issue
11eissn
0019-9567issn
1098-5522journal_volume
58pub_type
杂志文章abstract::Malaria toxin causes hypoglycemia and induction of tumor necrosis factor. Extracts of parasitized erythrocytes which were coeluted and copurified with one of the two subtypes of mammalian insulin-mimetic inositolphosphoglycans similarly induced fibroblast proliferation in the absence of serum. In addition, induction o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3438-3441.1996
更新日期:1996-08-01 00:00:00
abstract::Mycobacterial growth inhibitory factor (MycoIF), found in supernatant fluids of mouse spleen cell cultures that have been stimulated in vitro with homologous antigen, inhibited the intracellular multiplication of virulent tubercle bacilli within normal mouse peritoneal macrophages in vitro. Antigenically stimulated H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.833-840.1975
更新日期:1975-10-01 00:00:00
abstract::Binding of radiolabeled human fibrinogen and aggregated beta-microglobulin was measured in 60 strains of beta-hemolytic group B streptococci. Positive fibrinogen binding was detected in seven of the strains. Six of the group B strains showed an uptake of aggregated beta 2-microglobulin. Four individual strains carried...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.856-861.1981
更新日期:1981-03-01 00:00:00
abstract::In an effort to relate the protein profile to virulence, proteins from the cellular fractions and from culture supernatants of Streptococcus suis capsular type 2 strains from different geographical origins were compared by using Western blots (immunoblots). The protein profiles of the cellular fractions were similar f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1975-1979.1995
更新日期:1995-05-01 00:00:00
abstract::Actinobacillus pleuropneumoniae can use porcine transferrin as the sole source of iron. Two proteins with molecular masses of approximately 60 kDa (TfbA) and 110 kDa have been shown to specifically bind porcine transferrin; from the TfbA protein, three isoforms from A. pleuropneumoniae serotypes 1, 5, and 7 have been ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3846-3850.1995
更新日期:1995-10-01 00:00:00
abstract::Randomly bred guinea pigs were infected with Listeria monocytogenes using the intracardial, intravenous and intraperitoneal routes of infection. Doses of Listeria ranged from 5 to 1,000 x the 50% lethal dose based on the 50% lethal dose for intracardially injected Listeria. A complete necropsy was performed on all ani...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.916-924.1977
更新日期:1977-03-01 00:00:00
abstract::The goal of this study is to evaluate the contribution of mast cells to Helicobacter pylori immunity in a model of vaccine-induced protection. Mast cell-deficient Kitl(Sl)/Kitl(Sl-d) and control mice were immunized with H. pylori sonicate plus cholera toxin and challenged with H. pylori, and the bacterial loads, infla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00532-09
更新日期:2009-12-01 00:00:00
abstract::Specific proteolytic destruction of the human chemotaxin, C5a, is a property of group A and B streptococcal pathogens. Here we show that virulent group G streptococci from human sources also express C5a peptidase activity. The enzyme responsible for this activity is approximately the same size as and is antigenically ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2305-2310.1991
更新日期:1991-07-01 00:00:00
abstract::Histophilus somni is capable of intracellular survival within professional phagocytic cells, but the mechanism of survival is not understood. The Fic motif within the direct repeat (DR1)/DR2 domains of the IbpA fibrillary network protein of H. somni is cytotoxic to epithelial and phagocytic cells, which may interfere ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00365-18
更新日期:2018-11-20 00:00:00
abstract::We developed a human inhibition monoclonal enzyme-linked immunosorbent assay (HIMELISA) to investigate the human immune response to the lipooligosaccharides (LOS) of Neisseria meningitidis. Monoclonal antibodies (MAb) were used to define seven epitopes on four LOS molecules of a meningococcal strain (126E) previously ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2204-2213.1990
更新日期:1990-07-01 00:00:00
abstract::Chromatographic analysis, coupled to mass spectrometry with a high-resolution mass spectrometer, of materials isolated from skin lesions of patients with lepromatous leprosy allows the recognition of characteristic mycobacterial products, mycolic acids. This finding indicates that the "noncultivable" bacteria responsi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.236-239.1974
更新日期:1974-07-01 00:00:00
abstract::Extracytoplasmic proteins were released from Serpulina (Treponema) hyodysenteriae (strain B204) by treatment of whole cells with a nonionic detergent (Tween 20). Centrifugation of the Tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.142-148.1995
更新日期:1995-01-01 00:00:00
abstract::Purified cell walls representing a wide variety in teichoic acid and peptidoglycan structure prepared from eight different gram-positive bacterial species induced the production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 from human monocytes in the presence of 10% plasma or serum. Significant amounts...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2715-2721.1994
更新日期:1994-07-01 00:00:00
abstract::Toxin A (TcdA) and toxin B (TcdB) are major virulence factors of Clostridium difficile. These two toxins intoxicate cultured cells by similar mechanisms, and TcdB generally is more potent than TcdA in cultured cells. The exact reason for this difference is unclear. Here, we report that the cellular effects of TcdA can...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01577-08
更新日期:2009-06-01 00:00:00
abstract::Borrelia burgdorferi, a tick-borne bacterial pathogen, causes a disseminated infection involving multiple organs known as Lyme disease. Surface proteins can directly participate in microbial virulence by facilitating pathogen dissemination via interaction with host factors. We show here that a fraction of the B. burgd...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05671-11
更新日期:2012-01-01 00:00:00
abstract::We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We mea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.971-977.1981
更新日期:1981-03-01 00:00:00
abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
abstract::The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry and survival of two Streptococcus iniae serotypes. Efficient invasion by S. iniae occurred in both cells, but only the type II strain persisted in pronephros phagocytes for at least 48 h. Ex vivo ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.5.2318-2325.2003
更新日期:2003-05-01 00:00:00
abstract::Free-living amoebae of the genus Acanthamoeba can cause severe and chronic infections in humans, mainly localized in immune privileged sites, such as the brain and the eye. Monocytes/macrophages are thought to be involved in Acanthamoeba infections, but little is known about how these facultative parasites influence t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00345-16
更新日期:2016-09-19 00:00:00
abstract::Our previous animal studies showed that maternal Porphyromonas gingivalis infection in a subcutaneous chamber is associated with hepatic and uterine translocation, as well as systemic induction of maternal inflammatory responses, both of which were associated with fetal growth restriction (FGR). However, P. gingivalis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5163-5168.2003
更新日期:2003-09-01 00:00:00
abstract::Western blot (immunoblot) analysis of sera from cattle vaccinated with Brucella abortus S19 exhibit an elevated serologic response to Hsp62, the GroEL homolog (BaGroEL). Serologic screening of individual cows vaccinated with B. abortus S19 revealed no correlation between the immune response to BaGroEL and protection a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.10.4396-4400.1996
更新日期:1996-10-01 00:00:00
abstract::In this study, the efficacy of bactericidal/permeability-increasing protein (BPI) was assessed in a rat model of chronic otitis media with effusion. BPI injection prevented disturbance of the mucociliary clearance system of the middle ear. Hence, it is postulated that BPI can be a new therapy for chronic otitis media ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2992-2994.2000
更新日期:2000-05-01 00:00:00
abstract::The effects of treatment with live or inactivated Shope fibroma virus on host cell deoxyribonucleic acid (DNA) synthesis were determined. The incorporation of (3)H-thymidine into nuclear DNA was suppressed by both active and inactivated virus, although live virus was more effective. During the early phase of infection...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.4.532-538.1973
更新日期:1973-04-01 00:00:00
abstract::Pathogenic Leptospira spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized Leptospira interrogans catalase (KatE), the only annotated catalase found within pathogenic Leptospira species, by assessing its role in resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00466-12
更新日期:2012-11-01 00:00:00
abstract::Inbred BALB/c mice were either immunized with Triton X-100-extracted antigens of blood-stage Plasmodium yoelii or infected with P. yoelii and cured in three successive schedules. Whereas the immunized BALB/c became only partially protected from subsequent challenge infection with blood-stage P. yoelii, the convalescen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.3909-3916.1991
更新日期:1991-11-01 00:00:00
abstract::Most Helicobacter pylori strains express the BabA adhesin, which binds to ABO/Leb blood group antigens on gastric mucin and epithelial cells and is found more commonly in strains that cause peptic ulcers or gastric cancer, rather than asymptomatic infection. We and others have previously reported that in mice, gerbils...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00094-17
更新日期:2017-05-23 00:00:00
abstract::The objective of this study was to determine (i) if complementation of ureB-negative Helicobacter pylori restores colonization and (ii) if urease is a useful reporter for promoter activity in vivo. Strains used were M6, M6DeltaureB, and 10 recombinant derivatives of M6 or M6DeltaureB in which urease expression was und...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.771-778.2002
更新日期:2002-02-01 00:00:00
abstract::A synthetic medium capable of supporting growth of Vibrio parahaemolyticus is described. Growth yields and generation times were comparable to growth in a complex medium, although Kanagawa hemolysin was undetectable in the synthetic medium. Upon the addition of single amino acids to this synthetic medium, only D-trypt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
更新日期:1982-07-01 00:00:00
abstract::Immunochemical analyses of soluble antigens derived from microaerophilous stationary phase cultures of Babesia bovis demonstrated that at least three parasite antigens were released in vitro. These antigens have molecular weights within the range of 37,000 to 40,000, fast electrophoretic mobility in the albumin and al...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.358-361.1981
更新日期:1981-01-01 00:00:00
abstract::Internalization of Staphylococcus aureus in bovine endothelial cells (BEC) is increased by tumor necrosis factor alpha stimulation and NF-κB activation. Because the phosphoinositide-3-kinase (PI3K)-Akt signaling pathway also modulates NF-κB activity, we considered whether the internalization of S. aureus by BEC is ass...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05303-11
更新日期:2011-11-01 00:00:00