Abstract:
:Expression of ace (adhesin to collagen of Enterococcus faecalis), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46 °C. However, the mechanism of ace/Ace regulation under different conditions is still unknown. In this study, we identified a two-component regulatory system GrvRS as the main regulator of ace expression under these stress conditions. Using Northern hybridization and β-galactosidase assays of an ace promoter-lacZ fusion, we found transcription of ace to be virtually absent in a grvR deletion mutant under the conditions that increase ace expression in wild-type OG1RF and in the complemented strain. Moreover, a grvR mutant revealed decreased collagen binding and biofilm formation as well as attenuation in a murine urinary tract infection model. Here we show that GrvR plays a major role in control of ace expression and E. faecalis virulence.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Roh JH,Singh KV,La Rosa SL,Cohen AL,Murray BEdoi
10.1128/IAI.02587-14subject
Has Abstractpub_date
2015-01-01 00:00:00pages
389-95issue
1eissn
0019-9567issn
1098-5522pii
IAI.02587-14journal_volume
83pub_type
杂志文章abstract::Live attenuated bacteria hold great promise as multivalent mucosal vaccines against a variety of pathogens. A major challenge of this approach has been the successful delivery of sufficient amounts of vaccine antigens to adequately prime the immune system without overattenuating the live vaccine. Here we used a live a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02443-14
更新日期:2015-01-01 00:00:00
abstract::The immune mechanism by which hamsters acquire resistance to infection with Treponema pertenue, the causative agent of frambesia, or yaws, has not been elucidated. Serum or cells (spleen or lymph node) obtained from hamsters resistant to frambesial infection were transferred to normal syngenic recipients, who are subs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.430-435.1978
更新日期:1978-08-01 00:00:00
abstract::Complement receptor type 3 (CR3) was initially described as an opsonic receptor. Subsequently, CR3-mediated lectin-sugar recognition mechanisms have been shown to play a major role in the nonopsonic phagocytosis of several pathogens, among them Mycobacterium tuberculosis. Little is known about the binding and signal t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4736-4745.2000
更新日期:2000-08-01 00:00:00
abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth, and these molecules induce an inflammatory response in the human host. The proinflammatory molecules include peptidoglycan monomers, peptidoglycan dimers, and free peptides. These molecules can be released by the actions of lytic transglycosylases o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00833-18
更新日期:2019-01-24 00:00:00
abstract::Mice pretreated intraperitoneally with trehalose-6,6'-dimycolate (cord factor) were protected against an intraperitoneal challenge with Salmonella typhi strain Ty2 or Salmonella typhimurium strain SR 11. The nonspecific resistance to S. typhi and S. typhimurium was still detectable 7 and 14 days, respectively, after a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.5.1125-1129.1976
更新日期:1976-11-01 00:00:00
abstract::Severe combined immunodeficient (SCID)-beige mice inoculated with the intracellular parasite Eimeria papillata produced significantly more oocysts during primary infections than inoculated immunodeficient SCID mice. Therefore, the addition of the beige mutation, which detrimentally affects neutrophil and natural kille...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3165-3170.1997
更新日期:1997-08-01 00:00:00
abstract::The cell surface of Chlamydia psittaci seems important for establishing infection since (i) UV-treated elementary bodies (EB) attach to and are ingested by L cells and (ii) heat or antibody treatment decreases attachment to L cells and promotes the fusion of chlamydiae-containing phagosomes with lysosomes in macrophag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.741-751.1983
更新日期:1983-05-01 00:00:00
abstract::Cryptosporidial infections in severe combined immune deficient (SCID) mice produce a chronic disease state which in the later stages leads to extraintestinal involvement and hepatic dysfunction. To further characterize the infection dynamics in this model and monitor the changes in the hepatic system, a dose titration...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.5.1691-1695.1994
更新日期:1994-05-01 00:00:00
abstract::The regulation of innate immune responses to pathogens occurs through the interaction of Toll-like receptors (TLRs) with pathogen-associated molecular patterns and the activation of several signaling pathways whose contribution to the overall innate immune response to pathogens is poorly understood. We demonstrate a m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00492-07
更新日期:2007-10-01 00:00:00
abstract::Killing of Salmonella minnesota and Salmonella typhimurium S and R strains in serum of nonimmune humans and guinea pigs was drastically reduced in the selective absence of C1q, C1r, Ca2+, C4, or C2, the components of the classical complement pathway. Binding of C1 and C1q to the S form and six different core-deficient...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.935-939.1982
更新日期:1982-09-01 00:00:00
abstract::The production of interleukin-6 (IL-6) and its possible relationship to host resistance and inflammatory response to Pneumocystis carinii infection were examined in mice with severe combined immunodeficiency (SCID mice). IL-6 activity was detected in the serum and lungs of P. carinii-infected mice but not in mice free...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.97-102.1993
更新日期:1993-01-01 00:00:00
abstract::Rats and mice infected with Trypanosoma lewisi and Trypanosoma musculi, respectively, showed hyperreactivity to lipopolysaccharide (LPS) from gram-negative bacteria. Fatal shock could be precipitated with a dose of LPS 100 to 1,000 times less in infected compared with noninfected animals. In trypanosome-infected rats ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.501-506.1984
更新日期:1984-11-01 00:00:00
abstract::Human and rat C-reactive proteins, major acute-phase reactants, bind to sporozoites and inhibit their in vitro development in hepatocytes (A. Nussler, S. Pied, M. Pontet, F. Miltgen, L. Renia, M. Gentilini, and D. Mazier, Exp. Parasitol. 72:1-7, 1991, and S. Pied, A. Nussler, M. Pontet, F. Miltgen, H. Matile, P.-H. La...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.348-349.1993
更新日期:1993-01-01 00:00:00
abstract::Mortality associated with acute lung injury (ALI) induced by lipopolysaccharide (LPS) remains high in humans, warranting improved treatment and prevention strategies. ALI is characterized by the expression of proinflammatory mediators and extensive neutrophil influx into the lung, followed by severe lung damage. Under...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7247-7256.2004
更新日期:2004-12-01 00:00:00
abstract::Osteoarticular brucellosis is the most common complication in Brucella-infected humans regardless of age, sex, or immune status. The mechanism of bone destruction caused by Brucella species remained partially unknown due to the lack of a suitable animal model. Here, to study this complication, we explored the suitabil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00901-18
更新日期:2019-05-21 00:00:00
abstract::We studied the influence of staphylococcal toxic shock syndrome toxin 1 and streptococcal erythrogenic (pyrogenic) toxin A (ETA) on intact and digitonin-permeabilized human polymorphonuclear granulocytes (PMNs). As was shown by reversed-phase high-performance liquid chromatography analysis, toxic shock syndrome toxin ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.1055-1061.1993
更新日期:1993-03-01 00:00:00
abstract::Chagas' disease is a chronic infection caused by Trypanosoma cruzi and represents an important public health burden in Latin America. Frequently the disease evolves undetectable for decades, while in a significant fraction of the affected individuals it culminates in death by heart failure. Here, we describe a novel m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2350-2357.2004
更新日期:2004-04-01 00:00:00
abstract::ExoU is a potent effector protein that causes rapid host cell death upon injection by the type III secretion system of Pseudomonas aeruginosa. The N-terminal half of ExoU contains a patatin-like phospholipase A(2) (PLA(2)) domain that requires the host cell cofactor superoxide dismutase 1 (SOD1) for activation, while ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00223-10
更新日期:2010-08-01 00:00:00
abstract::Fibronectin binds to Streptococcus pyogenes, and this binding is inhibited by lipoteichoic acid (LTA). Previous studies have shown that LTA can be released from S. pyogenes by treatment with penicillin. Penicillin released LTA from both S. pyogenes and Staphylococcus aureus; however, the binding of fibronectin correla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.2.529-535.1986
更新日期:1986-02-01 00:00:00
abstract::We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken anti-SEB antibodies raised against the holotoxin could protect rhesus monkeys against aerosolized SEB. Although both fra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2278-2281.2002
更新日期:2002-05-01 00:00:00
abstract::The Gram-negative intracellular pathogen Burkholderia pseudomallei is the causative agent of melioidosis, an important cause of sepsis in Southeast Asia. Recognition of pathogen-associated molecular patterns by Toll-like receptors (TLRs) is essential for an appropriate immune response during pathogen invasion. In pati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00409-18
更新日期:2019-07-23 00:00:00
abstract::A murine respiratory infection model was used to study the mechanism of protective immunity to Bordetella pertussis. We found that nude mice, which are deficient in T cells, developed a persistent infection and failed to clear the bacteria after aerosol inoculation. In contrast, normal adult nonimmune mice cleared a r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.399-410.1993
更新日期:1993-02-01 00:00:00
abstract::Actinobacillus actinomycetemcomitans SUNY 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. Internalized organisms also egress from host cells into ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::To characterize the mechanism by which interleukin 10 (IL-10) inhibits Th1 responses to intracellular pathogens, we evaluated the interaction between IL-10 and Mycobacterium tuberculosis-induced gamma interferon (IFN-gamma) production by peripheral blood mononuclear cells from persons across the spectrum of tuberculou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.913-918.1996
更新日期:1996-03-01 00:00:00
abstract::The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping imm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.2.371-376.1994
更新日期:1994-02-01 00:00:00
abstract::Previous studies showed that Pseudomonas aeruginosa exotoxin A (PA toxin) catalyzes nicotinamide adenine dinucleotide (NAD)-dependent inhibition of protein synthesis in a rabbit reticulocyte lysate and transfer of radioactivity from [14C]adenine-labeled NAD to a protein having the same molecular weight as elongation f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.138-144.1977
更新日期:1977-01-01 00:00:00
abstract::The objective of this study was to identify disease-related changes in lymphocyte populations within ileal mucosae of calves with cryptosporidiosis. Groups of five neonatal calves were orally infected at 3 days of age with 10(8) oocysts and maintained in enteric-pathogen-free conditions until clinical disease was esta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.185-190.1997
更新日期:1997-01-01 00:00:00
abstract::Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high proportion of intravenously immunized mice survived virulent challenge, but subcutaneous vaccination was less effective. Vac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.798-803.1978
更新日期:1978-12-01 00:00:00
abstract::Temperate bacteriophage can transfer toxin-encoding genes between bacteria, often resulting in acquired pathogenicity. However, little is known regarding the effects of the eukaryotic host on the phage-pathogen interaction. Using Streptococcus pyogenes as a model, we demonstrate, both in vitro and in vivo, that the eu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3782-3786.2003
更新日期:2003-07-01 00:00:00
abstract::Several studies have demonstrated roles for eosinophils during innate and adaptive immune responses to helminth infections. However, evidence that eosinophils are capable of initiating an immune response to parasite antigens is lacking. The goal of the present in vitro study was to investigate the potential of eosinop...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02067-05
更新日期:2006-06-01 00:00:00