Abstract:
:Monoclonal antibodies were raised against a bactericidal protein fraction that was purified from an extract of bovine neutrophil granules and that was previously shown (A. Savoini, R. Marzari, L. Dolzani, D. Serranò, G. Graziosi, R. Gennaro, and D. Romeo, Antimicrob. Agents Chemother. 26:405-407, 1984) to inhibit bacterial DNA synthesis. One of these antibodies, BP97, was covalently linked to Affi-Gel 10 and was used for immunoaffinity chromatography of granule extracts. Elution of the bound proteins, followed by reversed-phase high-performance liquid chromatography, generated several peptides whose molecular weights fell in the range of 1,600 to 64,000 and which reacted to BP97 but not to other mouse monoclonal or polyclonal antibodies. The reaction to BP97 appeared to be specific, inasmuch as a full panel of cationic oligo- or polypeptides was not recognized by this monoclonal antibody. Among the purified granule polypeptides, the more cationic ones (with molecular weights of 4,300 to 8,000) inhibited the growth of Escherichia coli at a MIC of 12 to 50 micrograms/ml. In addition, a 1,600-molecular-weight, highly cationic peptide also inhibited the growth of Staphylococcus aureus and Staphylococcus epidermidis at MICs of 3 to 8 micrograms/ml.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Marzari R,Scaggiante B,Skerlavaj B,Bittolo M,Gennaro R,Romeo Ddoi
10.1128/IAI.56.8.2193-2197.1988subject
Has Abstractpub_date
1988-08-01 00:00:00pages
2193-7issue
8eissn
0019-9567issn
1098-5522journal_volume
56pub_type
杂志文章abstract::Ribosomes of strain NOR-7 of group B Neisseria meningitidis were isolated by a procedure that included treatment of the cells with sodium dodecyl sulfate, disruption in a French pressure cell, and differential centrifugation. These preparations consisted of 66% ribonucleic acid and 24% protein and sedimented as a sing...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.355-363.1972
更新日期:1972-09-01 00:00:00
abstract::Interleukin-1 receptor (IL1R)-associated kinase 4 (IRAK4) is a member of the IRAK family and has an important role in inducing the production of inflammatory mediators. This kinase is downstream of MyD88, an adaptor protein essential for Toll-like receptor (TLR) function. We investigated the role of this kinase in IRA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00328-12
更新日期:2012-12-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted obligate intracellular rickettsial agent, causes human monocytic ehrlichiosis. In recent reports, we described substantial advances in developing random and targeted gene disruption methods to investigate the functions of E. chaffeensis genes. We reported earlier that the Hima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00316-20
更新日期:2020-09-18 00:00:00
abstract::Brucella abortus endotoxin preparations, containing approximately 5 to 6% protein, induce strong immune and adjuvant immunoglobulin G (IgG) responses as compared with Escherichia coli endotoxin preparations, with equivalent amounts of protein, which induce responses in which IgM antibody predominates. Using an enzyme-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.728-734.1986
更新日期:1986-12-01 00:00:00
abstract::Lipid extracts derived from a group A Streptococcus, when preincubated with tumor cells before inoculation, were shown to prevent completely the development of Ehrlich ascites tumor in mice. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.5.705-706.1971
更新日期:1971-05-01 00:00:00
abstract::Urease from ureaplasmas was purified by immunoaffinity chromatography, and the N-terminal amino acid sequence was determined for two of the three subunits. These sequences were used to design primers for a polymerase chain reaction (PCR) that amplified most of the gene coding for one of the subunits. By using a novel ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2463-2469.1991
更新日期:1991-07-01 00:00:00
abstract::Intrakingdom cell-to-cell communication and interkingdom cell-to-cell communication play essential roles in the virulence of enterohemorrhagic Escherichia coli (EHEC). Four signals, autoinducer 2 (AI-2), AI-3, and the human hormones epinephrine and norepinephrine, are important in this communication. The effect of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00550-07
更新日期:2007-10-01 00:00:00
abstract::Moraxella catarrhalis expresses surface receptor proteins that specifically bind host transferrin (Tf) and lactoferrin (Lf) in the first step of the iron acquisition pathway. Acute- and convalescent-phase antisera from a series of patients with M. catarrhalis pulmonary infections were tested against Tf and Lf receptor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3793-3799.1999
更新日期:1999-08-01 00:00:00
abstract::Chromatographic analysis, coupled to mass spectrometry with a high-resolution mass spectrometer, of materials isolated from skin lesions of patients with lepromatous leprosy allows the recognition of characteristic mycobacterial products, mycolic acids. This finding indicates that the "noncultivable" bacteria responsi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.236-239.1974
更新日期:1974-07-01 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in controlling several aspects of immune responses, including the activation and differentiation of specific T cell subsets and antigen-presenting cells, thought to be relevant in the context of experimental Trypanosoma cruzi infec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00575-16
更新日期:2016-09-19 00:00:00
abstract::The nitrate dissimilation pathway is important for anaerobic growth in Pseudomonas aeruginosa. In addition, this pathway contributes to P. aeruginosa virulence by using the nematode Caenorhabditis elegans as a model host, as well as biofilm formation and motility. We used a set of nitrate dissimilation pathway mutants...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00822-09
更新日期:2009-10-01 00:00:00
abstract::Previous studies indicated that inorganic pyrophosphatase of Ascaris suum (AsPPase) plays an important role in larval survival in the host. Here we describe a precise role for AsPPase in larval molting and development and also describe the potential role of recombinant AsPPase (rAsPPase) in protective immunity to A. s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.1995-2004.2005
更新日期:2005-04-01 00:00:00
abstract::Using a murine respiratory infection model, we have demonstrated previously that infection with Bordetella pertussis or immunization with a whole-cell pertussis vaccine induced antigen-specific Th1 cells, which conferred a high level of protection against aerosol challenge. In contrast, immunization with an acellular ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5295-5301.1996
更新日期:1996-12-01 00:00:00
abstract::Antimicrobial peptides, such as defensins or cathelicidins, are effector substances of the innate immune system and are thought to have antimicrobial properties that contribute to host defense. The evidence that vertebrate antimicrobial peptides contribute to innate immunity in vivo is based on their expression patter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6084-6089.1999
更新日期:1999-11-01 00:00:00
abstract::Helicobacter mustelae, like Helicobacter pylori, possesses two flagellin proteins, FlaA and FlaB. Isogenic mutant strains of H. mustelae have been constructed by disruption of the flaA or flaB gene with a kanamycin resistance cassette or by introduction of both a kanamycin and a chloramphenicol resistance gene to prod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1962-1966.1997
更新日期:1997-05-01 00:00:00
abstract::One-day-old chicks were infected orally with two strains of weakly hemolytic spirochetes isolated from a human and a pig with intestinal spirochetosis. These spirochetosis both colonized birds, attached end-on to their cecal enterocytes, induced watery diarrhea, and significantly depressed growth rates. Cultures of Se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3705-3710.1995
更新日期:1995-09-01 00:00:00
abstract::Species-specific antibody epitopes within several major immunoreactive protein orthologs of Ehrlichia species have recently been identified and molecularly characterized. In this study, dominant B-cell epitopes within the acidic (pI 5.35) ankyrin repeat-containing 200-kDa major immunoreactive protein (gp200) of Ehrlic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00041-07
更新日期:2007-10-01 00:00:00
abstract::We isolated a monoclonal antibody directed against the ferric complex of aerobactin purified from Escherichia coli KH576. This antibody, which we designated MAb AERO1, was identified as an immunoglobulin G, subtype 2. A competitive enzyme-linked immunosorbent assay with MAb AERO1 had a limit of 10 nM for the detection...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.3.768-772.1992
更新日期:1992-03-01 00:00:00
abstract::Pathogenic leptospires have the ability to survive and disseminate to multiple organs after penetrating the host. Several pathogens, including spirochetes, have been shown to express surface proteins that interact with the extracellular matrix (ECM). This adhesin-mediated binding process seems to be a crucial step in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00460-06
更新日期:2006-11-01 00:00:00
abstract::Leishmania-infected macrophages are potential antigen-presenting cells for CD4+ T lymphocytes, which recognize parasite antigens bound to major histocompatibility complex class II molecules (Ia). However, the intracellular sites where Ia and antigens may interact are far from clear, since parasites grow within the mod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.764-775.1991
更新日期:1991-03-01 00:00:00
abstract::Serologic parameters of kala-azar were evaluated by Western blot analysis. Sera from kala-azar patients with confirmed diagnoses were screened for immunoglobulin G (IgG) and IgG subclass-specific reactivity against Leishmania donovani membrane antigen (LAg). Heterogeneous LAg-specific IgG reactivity with numerous prot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.863-870.2004
更新日期:2004-02-01 00:00:00
abstract::Exposure of PAM cells, a spontaneously transformed mouse cell line, to brain homogenates from mice infected with scrapie caused a relative decrease in total cell yield, which persisted from passage 2 or 3 to passage 18 after treatment. The effect was elicited by each of the eight independent scrapie isolates tested. L...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.1.163-167.1976
更新日期:1976-07-01 00:00:00
abstract::Previous studies showed that Pseudomonas aeruginosa exotoxin A (PA toxin) catalyzes nicotinamide adenine dinucleotide (NAD)-dependent inhibition of protein synthesis in a rabbit reticulocyte lysate and transfer of radioactivity from [14C]adenine-labeled NAD to a protein having the same molecular weight as elongation f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.138-144.1977
更新日期:1977-01-01 00:00:00
abstract::Chlamydia pneumoniae is a causative agent for many respiratory infections and has been associated with cardiovascular diseases in humans. The pathogenicity of C. pneumoniae is thought to depend on its ability to cause persistent infection and to evade host defense. Genome sequence analysis indicates that C. pneumoniae...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.345-349.2002
更新日期:2002-01-01 00:00:00
abstract::Human immunodeficiency virus (HIV)-infected individuals are predisposed to recurrent oral candidiasis, and, although it has been assumed that this is because of deficient mucosal immune responses, this has not been properly established. The present study aimed to compare the concentrations and secretion rates of immun...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.892-896.1994
更新日期:1994-03-01 00:00:00
abstract::Anti-rabbit alveolar macrophage goat serum (AMS), adsorbed of hemolysins and hemagglutinins, exhibited complement-dependent cytotoxicity for the following rabbit target cells: alveolar macrophages, thymocytes, and kidney (RK-13) cells. The intravenous injection of AMS, sufficient to reduce markedly the dermal tubercul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.6.810-814.1970
更新日期:1970-12-01 00:00:00
abstract::We have determined the nucleotide sequence of the 1.4-kilobase DNA fragment containing the plasminogen activator gene (pla) of Yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. The sequence revealed the presence of a 936-base-pair open reading frame that constitutes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1517-1523.1989
更新日期:1989-05-01 00:00:00
abstract::Enterotoxigenic Escherichia coli (ETEC) is a leading diarrheagenic bacterial pathogen among travelers and children in resource-limited regions. Adherence to host intestinal cells mediated by ETEC fimbriae is believed to be a critical first step in ETEC pathogenesis. These fimbriae are categorized into related classes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00246-20
更新日期:2020-10-19 00:00:00
abstract::Chlamydia trachomatis is an obligately intracellular bacterial parasite of eucaryotic cells that undergoes a biphasic life cycle within a parasitophorous vacuole (PV) called an inclusion. The parasitophorous vacuolar membrane (PVM) constitutes a barrier between the replicating bacteria and the nutrient-rich environmen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.1088-1094.1997
更新日期:1997-03-01 00:00:00
abstract::Science has always been a competitive undertaking. Despite recognition of the benefits of cooperation and team science, reduced availability of funding and jobs has made science more competitive than ever. Here we consider the benefits of competition in providing incentives to scientists and the adverse effects of com...
journal_title:Infection and immunity
pub_type: 社论
doi:10.1128/IAI.02939-14
更新日期:2015-04-01 00:00:00