Abstract:
:A hemagglutinin with an M(r) of 67,000 (pMGA) from Mycoplasma gallisepticum S6 was purified by using monoclonal antibody affinity chromatography. Purified pMGA was treated with a number of enzymes, the resultant peptides were purified, and their amino acid sequence was determined by using an Applied Biosystems (model 471A) protein sequencer. The DNA sequence encoding two peptides was used to dictate the sequences of synthetic oligonucleotides which were used to screen a library of EcoRI-cut M. gallisepticum DNA in pUC18. A clone reactive to both probes was isolated and found to contain a recombinant insert of 10 kb. The clone was mapped by using restriction endonucleases and fragments subcloned into pUC18 for DNA sequencing. Analysis of part of the DNA sequence revealed an open reading frame containing 1,941 nucleotides which encoded 647 amino acids. The amino terminus was preceded by a putative leader sequence of 25 amino acids. A promoter region preceding the putative start codon GUG was also located. This gene would encode a mature protein of 67,660 Da. There were a number of differences between the predicted amino acid sequence and that determined by direct peptide sequencing. Also, two tryptic peptides of pMGA were not found in the DNA sequence. This suggested that the cloned gene did not encode pMGA but did encode a homolog (pMGA1.2). Furthermore, downstream of pMGA1.2 was a region of DNA encoding a leader sequence followed by an amino acid sequence with high homology to that encoded by the pMGA1.2 gene. The presence within M. gallisepticum of a family of pMGA genes is inferred from the DNA sequence and Southern transfer data. A possible role for this gene family in immune evasion is discussed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Markham PF,Glew MD,Whithear KG,Walker IDdoi
10.1128/IAI.61.3.903-909.1993subject
Has Abstractpub_date
1993-03-01 00:00:00pages
903-9issue
3eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::Trehalose 6,6'-dimycolate (TDM) plays important roles in the development of granulomatous inflammation during infection with Mycobacterium spp., Rhodococcus spp., etc. To reveal the augmenting effect of TDM on vascular endothelial growth factor (VEGF) production and neovascularization, we investigated murine granuloma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2043-2052.2000
更新日期:2000-04-01 00:00:00
abstract::Streptococcus sanguis 804 (NCTC 10904) was grown ih batch culture at constant pH. and the glucosyltransferase activity of the supernatant was assayed over a 40-h growth period. The optimum pH for enzyme production was 7.0 to 7.2. During growth of the culture, three reproducible phases of enzyme activity were observed....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.50-58.1977
更新日期:1977-01-01 00:00:00
abstract::The susceptibility of a few strains of mice to a subcutaneous injection of Leishmania tropica major, the causative agent of cutaneous leishmaniasis in humans, was studied. The infection in six strains (CBA, AKR/J, AKR/cu, C57BL/6, A/J, and C3H) remained cutaneous, and the animals recovered within 3 to 4 months. In con...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.611-614.1979
更新日期:1979-11-01 00:00:00
abstract::A canine distemper virus (CDV), DESIGNATED R252, originally recovered from a dog with demyelinating encephalomyelitis was shown to reproduce this disease in gnotobiotic dogs with a high incidence as compared to other CDV strains, which produced an acute fatal infection. In this investigation, R252 was propagated for t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.835-844.1975
更新日期:1975-04-01 00:00:00
abstract::Bactericidal activity and binding of a 57,000-dalton cationic antimicrobial neutrophil granule protein (CAP57) are determined by the presence on bacteria of O-antigen polysaccharide chains and the availability of negatively charged groups in the lipid A region, the inner core region, or both regions of lipopolysacchar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1589-1592.1988
更新日期:1988-06-01 00:00:00
abstract::Individual strains of Staphylococcus aureus have different capacities to become internalized by osteoblasts. Here we report that the levels of sigma(B) expressed by S. aureus correlate with the capacity of this bacterium to be internalized by osteoblasts. However, sigma(B) is not essential for internalization and does...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4167-4170.2003
更新日期:2003-07-01 00:00:00
abstract::Athymic nude mice (nu/nu), heterozygous litter mates (nu/+), and thymic reconstituted homozygous animals (nu/nu TxR) were infected with Trypanosoma rhodesiense. A reduced parasitemia and prolonged survival were observed in the nu/nu animals after infection and cure or after immunization with irradiated organisms. Thes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.3.714-720.1978
更新日期:1978-06-01 00:00:00
abstract::Infantile gastroenteritis virus (orbi-group) recovered from stools of infants with acute nonbacterial gastroenteritis was administered per os to germfree and conventional piglets. Virus was found subsequently in stools and in the mucosal epithelial cells of the small intestine of these animals. Some animals developed ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.6.1276-1280.1975
更新日期:1975-12-01 00:00:00
abstract::Bioluminescent reporter assays have been widely used to study the effect of Toxoplasma gondii on host gene expression. In the present study, we extend these studies by engineering novel reporter cell lines containing a gamma-activated sequence (GAS) element driving firefly luciferase (FLUC). In RAW264.7 macrophages, T...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00077-16
更新日期:2016-06-23 00:00:00
abstract::The tetraspanins are a superfamily of transmembrane proteins with diverse functions and can form extended microdomains within the plasma membrane in conjunction with partner proteins, which probably includes receptors for bacterial adhesins. Neisseria meningitidis, the causative agent of meningococcal disease, attache...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01354-10
更新日期:2011-06-01 00:00:00
abstract::Humoral and cell-mediated immune responses to varicella-zoster (V-Z) virus were assessed in patients during and after V-Z infections. Ongoing V-Z infections was associated with minimal cellular immunity but not necessarily with poor humoral immunity. Recovery from V-Z infection was associated with a vigorous cellular ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.170-174.1979
更新日期:1979-07-01 00:00:00
abstract::The antigenicities of Mycobacterium bovis strain BCG and an isoniazid (INH) - resistant mutant were compared. Sonic extracts of heat-killed, washed bacteria were injected into rabbits, and antisera were produced (anti-BCG and anti-BCG-R). The sonic extracts were also subjected to ultracentrifugation, and test antigens...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.4.524-529.1971
更新日期:1971-04-01 00:00:00
abstract::Exoenzyme Y (ExoY) is a type III secretion system effector found in 90% of the Pseudomonas aeruginosa isolates. Although it is known that ExoY is a soluble nucleotidyl cyclase that increases the cytoplasmic levels of nucleoside 3',5'-cyclic monophosphates (cNMPs) to mediate endothelial Tau phosphorylation and permeabi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00239-17
更新日期:2017-08-18 00:00:00
abstract::Previous studies have reported that human vascular endothelial cells lack the membrane-bound lipopolysaccharide (LPS) receptor, CD14 (mCD14). By optimizing assay conditions, including the selection of anti-CD14 monoclonal antibody, we now demonstrate that human umbilical vein endothelial cells (HUVEC) express CD14 on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.479-485.2001
更新日期:2001-01-01 00:00:00
abstract::YopE is a virulence factor that is secreted into host cells infected by Yersinia species. The YopE C-terminal domain has GTPase-activating protein (GAP) activity. The YopE N-terminal domain contains an epitope that is an immunodominant CD8(+) T cell antigen during primary infection of C57BL/6 mice with Yersinia pseudo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01687-14
更新日期:2014-07-01 00:00:00
abstract::Local gingival immunization was attempted in an effort to confine the immune response to the oral cavity and bypass the systemic immune response. A low-molecular-weight (3.8K) streptococcal antigen (SA) I/II was applied 10 times over a period of 1 year to the gingival crevices of rhesus monkeys. The antigen was mainta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.682-687.1986
更新日期:1986-06-01 00:00:00
abstract::Infection with Trypanosoma cruzi, the agent of Chagas' disease, may induce antibodies and T cells reactive with self antigens (autoimmunity). Because autoimmunity is generally thought to develop during the chronic phase of infection, one hypothesis is that autoimmunity develops only after long-term, low-level stimulat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5643-5649.2001
更新日期:2001-09-01 00:00:00
abstract::Culture fluids after growth of Mycobacterium bovis BCG on Sauton medium contain actively secreted proteins and proteins released by bacterial lysis. BCG culture fluids and sonicates of Mycobacterium tuberculosis and Mycobacterium paratuberculosis were tested after separation by gel filtration and sodium dodecyl sulfat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.289-296.1998
更新日期:1998-01-01 00:00:00
abstract::The purpose of the present study was to analyze Chlamydia pneumoniae-induced, antigen-specific, cell-mediated immunity. Peripheral blood mononuclear cells of four persons infected with C. pneumoniae Kajaani 6 and 17 healthy volunteers were stimulated with antigen composed of whole elementary bodies of C. pneumoniae Ka...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2196-2199.1993
更新日期:1993-05-01 00:00:00
abstract::Natural bactericidal resistance to Mycobacterium bovis BCG is under the control of a single gene, designated Bcg. Lung granuloma formation in susceptible (Bcgs) and resistant (Bcgr) mice was studied in two sets of Bcg-congenic systems, namely, the BALB/c (Bcgs)-C.D2 (BALB/c.Bcgr) pair and the B10.A (Bcgs)-B10.Ar (Bcgr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1210-1216.1990
更新日期:1990-05-01 00:00:00
abstract::Sterile sonic-treated materials of pneumococci grown in media containing N-acetylmannosamine as the carbohydrate source were lethal for mice. Purification of the toxic factor led to its identification as the enzyme neuraminidase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.115-117.1970
更新日期:1970-07-01 00:00:00
abstract::We report the immunizing capacity of Salmonella enteritidis AL1192, a strain that has been cured of a 36-megadalton plasmid, to protect ddY mice against subsequent challenge with virulent salmonellas. This strain, which was given subcutaneously at a dose of 10(6) organisms, provided significant protection against oral...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.586-587.1985
更新日期:1985-11-01 00:00:00
abstract::Decay-accelerating factor (DAF), a complement regulatory protein, also serves as a receptor for Dr adhesin-bearing Escherichia coli. The repeat three of DAF was shown to be important in Dr adhesin binding and complement regulation. However, Dr adhesins do not bind to red blood cells with the rare polymorphism of DAF, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4485-4493.2002
更新日期:2002-08-01 00:00:00
abstract::Klebsiella pneumoniae strains typically express both smooth lipopolysaccharide (LPS) with O antigen molecules and capsule polysaccharide (K antigen) on the surface. A single mutation in a gene that codes for a UDP galacturonate 4-epimerase (uge) renders a strain with the O-:K- phenotype (lack of capsule and LPS withou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.54-61.2004
更新日期:2004-01-01 00:00:00
abstract::The concept that relates low virulence of certain alphaviruses to low viremia and efficient vascular clearance of virus was tested in guinea pigs. Previously published studies with hamsters suggested that virulent strains maintain high viremias primarily because they are cleared inefficiently from the blood. In the pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.356-360.1977
更新日期:1977-08-01 00:00:00
abstract::Intra-abdominal polymicrobial infections cause significant morbidity and mortality. An experimental mouse model of Candida albicans-Staphylococcus aureus intra-abdominal infection (IAI) results in 100% mortality by 48 to 72 h postinoculation, while monomicrobial infections are avirulent. Mortality is associated with r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01059-15
更新日期:2015-10-19 00:00:00
abstract::Susceptibility to the lethal toxicity of endotoxin in mice pretreated with emulsified trehalose-6,6'-dimycolate was enhanced by (i) increasing the oil concentration while keeping the Tween concentration constant; and (ii) decreasing the Tween concentration, while keeping the oil concentration constant. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.571-572.1979
更新日期:1979-05-01 00:00:00
abstract::Outer membrane porin genes of Salmonella typhimurium, including ompC, ompF, and tppB, are regulated by the products of ompB, a two-component regulatory locus encoding OmpR and EnvZ. S. typhimurium ompR mutants are attenuated in mice, but to date no one has studied the intracellular trafficking of S. typhimurium porin-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1806-1811.1998
更新日期:1998-04-01 00:00:00
abstract::Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the present study, we used a set of isogenic Candida strains lacking one or more of the drug efflux pumps Cdr1p, Cdr2p, and Mdr1p to determine their role in FLU resistance of b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4333-4340.2003
更新日期:2003-08-01 00:00:00
abstract::The effect of a deficiency in the C5 component of complement on the pathophyisology of infection with the fungal pathogen Candida albicans was studied by using the A/J inbred mouse strain and the BcA17 congenic mouse strain. Acute infection caused by intravenous injection of C. albicans blastospores is associated with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00159-06
更新日期:2006-08-01 00:00:00