Abstract:
:The antigenicities of Mycobacterium bovis strain BCG and an isoniazid (INH) - resistant mutant were compared. Sonic extracts of heat-killed, washed bacteria were injected into rabbits, and antisera were produced (anti-BCG and anti-BCG-R). The sonic extracts were also subjected to ultracentrifugation, and test antigens were prepared from the resulting supernatants (BCG and BCG-R). The antigens were labeled with (131)I, and the antisera were tested for their capacity to bind (131)I-BCG and (131)I-BCG-R by precipitating (131)I-antigen-antibody complexes with anti-rabbit gamma globulin. Significant differences were detected between the two test antigens. Immunodiffusion studies with unabsorbed antisera and antisera absorbed with BCG and BCG-R suggested that an antigenic component in BCG is lacking or diminished in BCG-R and that anti-BCG has antibodies not found in anti-BCG-R. These quantitative and qualitative differences were confirmed by inhibition studies. Complete inhibition was achieved in all systems by both test antigens when either was added in sufficient concentration. As expected, BCG-R inhibited the binding of (131)I-BCG-R by anti-BCG-R or anti-BCG (or both) to a greater extent than BCG, and BCG was a better inhibitor in an (131)I-BCG-anti-BCG-R system. However, in an (131)I-BCG-anti-BCG test system, BCG was the better inhibitor only at high concentrations. It was concluded that the test antigens possess different proportions of identical or cross-reacting components and that INH-sensitive organisms possess a component(s) that is diminished or absent in the INH-resistant mutant. Whether this component(s) is responsible for INH-resistance is not known.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Minden P,McClatchy JK,Bardana EJ,Farr RSdoi
10.1128/IAI.3.4.524-529.1971keywords:
subject
Has Abstractpub_date
1971-04-01 00:00:00pages
524-9issue
4eissn
0019-9567issn
1098-5522journal_volume
3pub_type
杂志文章abstract::Random minitransposon mutagenesis was used to identify genes involved in the survival of Bordetella bronchiseptica within eukaryotic cells. One of the mutants which exhibited a reduced ability to survive intracellularly harbored a minitransposon insertion in a locus (ris) which displays a high degree of homology to tw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.4640-4650.1998
更新日期:1998-10-01 00:00:00
abstract::Rabbit endogenous pyrogens were of about the same molecular size, but showed considerable heterogeneity of their isoelectric points. We attempted to show that this heterogeneity was attributable to variable glycosylation of a single polypeptide chain. When peritoneal exudate cells were stimulated to make pyrogens in t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.184-191.1981
更新日期:1981-10-01 00:00:00
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doi:10.1128/IAI.00720-06
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.10.5603-5609.2000
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journal_title:Infection and immunity
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doi:10.1128/IAI.67.1.230-236.1999
更新日期:1999-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1144-1151.1983
更新日期:1983-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00025-07
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.538-557.1979
更新日期:1979-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00237-18
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pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.11.2734-2740.1987
更新日期:1987-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00850-16
更新日期:2017-03-23 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.897-901.1986
更新日期:1986-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1093-1100.1990
更新日期:1990-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.277-281.1985
更新日期:1985-01-01 00:00:00
abstract::Chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. By stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. However, in patient this condition is most typically found in lungs damaged by other factors, such ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2936-2942.1992
更新日期:1992-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.583-586.1972
更新日期:1972-10-01 00:00:00
abstract::Human galectin-3 binds to the surface of Trypanosoma cruzi trypomastigotes and human coronary artery smooth muscle (CASM) cells. CASM cells express galectin-3 on their surface and secrete it. Exogenous galectin-3 increased the binding of T. cruzi to CASM cells. Trypanosome binding to CASM cells was enhanced when eithe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.11.6717-6721.2004
更新日期:2004-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.3.256-263.1971
更新日期:1971-09-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.411-415.1978
更新日期:1978-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.3.1211-1215.1981
更新日期:1981-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.213-217.1998
更新日期:1998-01-01 00:00:00
abstract::Staphylococcus aureus is a pathogen often found in pneumonia and sepsis. In the context of the resistance of this organism to conventional antibiotics, an understanding of the regulation of natural endogenous antimicrobial molecules is of paramount importance. Previous studies have shown that both human and mouse airw...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3609-3617.2005
更新日期:2005-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.791-798.1975
更新日期:1975-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1489-1495.1990
更新日期:1990-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.159-165.1992
更新日期:1992-01-01 00:00:00
abstract::Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The consequences of vaccination were evaluated in terms of the growth of BCG in the lungs and spleen, lung and spleen weigh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Beta-1,2-linked mannosides (beta-Mans) are believed to contribute to Candida albicans virulence. The presence of beta-Mans has been chemically established for two molecules (phosphopeptidomannan [PPM] and phospholipomannan) that are noncovalently linked to the cell wall, where they correspond to specific epitopes. How...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00368-08
更新日期:2008-10-01 00:00:00