Abstract:
:Reverse transcriptase PCR analyses have demonstrated that open reading frames (ORFs) PM0298, PM0299, and PM0300 of the animal pathogen Pasteurella multocida constitute a single transcriptional unit. By cloning and overexpression studies in Escherichia coli cells, the product of ORF PM0300 was shown to bind hemoglobin in vitro; this ORF was therefore designated hgbA. In vitro and in vivo quantitative assays demonstrated that the P. multocida hgbA mutant bound hemoglobin to the same extent as the wild-type strain, although the adsorption kinetics was slightly slower for the hgbA cells. In agreement with this, the virulence of P. multocida hgbA cells was not affected, suggesting that other functional hemoglobin receptor proteins must be present in this organism. On the other hand, P. multocida mutants defective in PM0298 and PM0299 could be isolated only when a plasmid containing an intact copy of the gene was present in the cells, suggesting that these genes are essential for the viability of this bacterial pathogen. By adapting the recombinase-based expression technology in vivo to P. multocida, we also demonstrated that the transcriptional PM0298-PM0299-hgbA unit is iron regulated and that its expression is triggered in the first 2 h following infection in a mouse model. Furthermore, hybridization experiments showed that the hgbA gene is widespread in P. multocida strains regardless of their serotype or the animal from which they were isolated.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Bosch M,Garrido ME,Llagostera M,Pérez De Rozas AM,Badiola I,Barbé Jdoi
10.1128/iai.70.11.5955-5964.2002keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
5955-64issue
11eissn
0019-9567issn
1098-5522journal_volume
70pub_type
杂志文章abstract::Shiga toxin-producing Escherichia coli (STEC) is a food-borne pathogen with a low infectious dose that colonizes the colon in humans and can cause severe clinical manifestations such as hemolytic-uremic syndrome. The urease enzyme, encoded in the STEC chromosome, has been demonstrated to act as a virulence factor in o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00210-12
更新日期:2012-08-01 00:00:00
abstract::An attenuated Salmonella typhi Ty21a vaccine was administered to 18 infants and toddlers (less than or equal to 24 months old) to determine its safety and immunogenicity. The vaccination (10(9) CFU per dose, three doses) was well tolerated. However, after the vaccination there was no evidence of a humoral or cellular ...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1128/IAI.59.11.4291-4293.1991
更新日期:1991-11-01 00:00:00
abstract::Here we describe a protocol for purifying Borrelia burgdorferi from feeding ticks by velocity centrifugation and Percoll density gradient centrifugation. The purified spirochetes were motile and 10- to 20-fold purer than the bacteria in crude tick homogenates. The purified bacteria were present in sufficient quantity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3536-3541.2001
更新日期:2001-06-01 00:00:00
abstract::Campylobacter rectus is a putative periodontopathogen which expresses a proteinaceous surface layer (S-layer) external to the outer membrane. S-layers are considered to play a protective role for the microorganism in hostile environments. The S-layer proteins from six different C. rectus strains (five human isolates a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.478-483.1997
更新日期:1997-02-01 00:00:00
abstract::A total of 224 strains were serogrouped by coagglutination (COA) and serotyped by protein I enzyme-linked immunosorbent assay (ELISA). Of these strains, 61 were from patients with disseminated gonococcal infection, 21 were from patients with pelvic inflammatory disease, and 115 were from patients with uncomplicated go...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.229-239.1982
更新日期:1982-01-01 00:00:00
abstract::An important role for immunoglobulin M (IgM) during early acute virulent Toxoplasma gondii infection was identified using IgM-/- mice that lack surface and secretory IgM but maintain normal B-cell functionality and isotype class switching. Following intraperitoneal inoculation with the virulent RH strain, IgM-/- mice ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.12.8060-8068.2005
更新日期:2005-12-01 00:00:00
abstract::It is well established that activating-type Fc receptors for immunoglobulin G (FcgammaR), such as FcgammaRI and FcgammaRIII, are essential for inducing inflammatory responses. On the other hand, a unique inhibitory FcgammaR, FcgammaRIIB, inhibits intracellular signaling upon engagement of immunoglobulin G-immune compl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5048-5052.2005
更新日期:2005-08-01 00:00:00
abstract::We investigated the effect of the extracellular protease of Serratia marcescens on human serum constituents such as immunoglobulins, fibronectin, alpha 1-protease inhibitor, alpha 2-macroglobulin, lysozyme, and transferrin. At a very low concentration of Serratia 56-kilodalton protease (56K protease), purified human p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.3.522-529.1986
更新日期:1986-09-01 00:00:00
abstract::Mycoplasma arthritidis causes acute polyarthritis in rats and chronic proliferative arthritis in mice. M. arthritidis-induced arthritis serves as a model for arthritis caused by infectious agents and as a model for examining the role of the superantigen MAM (M. arthritidis T-cell mitogen) in the development of autoimm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.245-249.2005
更新日期:2005-01-01 00:00:00
abstract::To assess the humoral immunological responses at the subclass level in shigellosis, specific antibody responses against Shigella dysenteriae 1 lipopolysaccharide (LPS), S. flexneri Y LPS, invasion plasmid-coded protein antigens (Ipa), and Shiga toxin were analyzed. Antibody responses of 41 patients with S. dysenteriae...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.2054-2061.1995
更新日期:1995-05-01 00:00:00
abstract::We undertook a morphological and immunochemical comparison of purified outer membrane antigens from oral and nonoral isolates of Bacteroides asaccharolyticus. Electron micrographs of thin sections of whole bacteria revealed a compact, electron-dense capsule external to the outer membrane of oral strains. A loose, web-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.589-596.1980
更新日期:1980-02-01 00:00:00
abstract::Altogether 171 serum specimens from 58 patients with heterophil antibody-positive infectious monomucleosis were studied for antibody response to Epstein-Barr virus (EBV). The sera were tested for fluorescent immunoglobulin G (IgG) and IgM gel-precipitating (GP) and complement-fixing (CF) antibodies to EBV. All 58 pati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.42-51.1975
更新日期:1975-01-01 00:00:00
abstract::Human genital Chlamydia infection is a major public health concern due to the serious reproductive system complications. Chlamydia binds several receptor tyrosine kinases (RTKs) on host cells, including the epidermal growth factor receptor (EGFR), and activates cellular signaling cascades for host invasion, cytoskelet...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00819-19
更新日期:2020-03-23 00:00:00
abstract::Candida-associated denture stomatitis (DS) is a persistent and chronic oral infection of the denture-bearing palatal mucosa. DS stems from the ability of the fungal opportunistic pathogen Candida albicans to adhere to denture material and invade palatal tissue. Although DS is the most prevalent form of oral candidiasi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00617-19
更新日期:2019-11-18 00:00:00
abstract::Mice were vaccinated with plasmid DNA (pDNA) encoding antigen 85A (Ag85A), Ag85B, or PstS-3 from Mycobacterium tuberculosis either in saline or formulated for intramuscular injections in VC1052:DPyPE (aminopropyl-dimethyl-myristoleyloxy-propanaminium bromide-diphytanoylphosphatidyl-ethanolamine) (Vaxfectin; Vical, Inc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3681-3688.2002
更新日期:2002-07-01 00:00:00
abstract::PspC was found to bind human complement factor H (FH) by Western blot analysis of D39 (pspC(+)) and an isogenic mutant TRE108 (pspC). We confirmed that PspA does not bind FH, while purified PspC binds FH very strongly. The binding of FH to exponentially growing pneumococci varied among different isolates when analyzed...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3435-3437.2001
更新日期:2001-05-01 00:00:00
abstract::Membrane glycolipids contain the lactose sequence (galactose linked to glucose), and the oligosaccharide is variously extended such that there is a cell-type-specific repertoire. In this study, binding of Pseudomonas aeruginosa M35 to lipid-linked lactose (Gal beta 1-4Glc [structure 1]), lacto-N-neotetraose (Gal beta ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5078-5084.1992
更新日期:1992-12-01 00:00:00
abstract::Lewis (Le) antigens have been implicated in the pathogenesis of atrophic gastritis and gastric cancer in the setting of Helicobacter pylori infection, and H. pylori-induced anti-Le antibodies have been described that cross-react with the gastric mucosa of both mice and humans. The aim of this study was to examine the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.4774-4781.2001
更新日期:2001-08-01 00:00:00
abstract::Studies concerned with the mechanisms responsible for relative resistance or susceptibility of strains of inbred mice to Trypanosoma musculi infections are presented. Treatment with 400 rads of ionizing radiation, silica dust, or trypan blue (reticuloendothelial blocking agents) rendered C3H mice unable to control the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.6.1647-1655.1989
更新日期:1989-06-01 00:00:00
abstract::Feline leukemia-sarcoma group-specific antigens were located in human embryo cells infected with feline leukemia and feline sarcoma viruses. This was done by using the fluorescent-antibody and enzyme-labeled antibody techniques at both light and electron microscopic levels. The antigens were found to be exclusively in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.3.400-405.1972
更新日期:1972-03-01 00:00:00
abstract::Toxoplasma gondii induces the expression of proinflammatory cytokines, reorganizes organelles, scavenges nutrients, and inhibits apoptosis in infected host cells. We used a cDNA microarray of 420 annotated porcine expressed sequence tags to analyze the molecular basis of these changes at eight time points over a 72-ho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00386-05
更新日期:2006-07-01 00:00:00
abstract::The humoral and cell-mediated immune responses of murine lymphocytes exposed to lead in vivo and in vitro were investigated. In vivo Pb was administered via the drinking water (0 to 10 mM) for 1 to 10 weeks. In vivo exposure of the mice to Pb did not alter significantly their plaque-forming cell response to sheep eryt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.136-143.1981
更新日期:1981-01-01 00:00:00
abstract::Spleen cell cultures from guinea pigs given legionella pneumophila vaccine in complete Freund adjuvant or as a sublethal infection were inhibited in their migration activity in vitro when incubated with specific antigen. Both direct and indirect migration inhibition assays revealed sensitization of the guinea pigs to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.3.1132-1137.1983
更新日期:1983-09-01 00:00:00
abstract::A major obstacle to studying the functions of particular gene products in the mouse-tick infectious cycle of Borrelia burgdorferi has been an inability to knock out genes in pathogenic strains. Here, we investigated conditions for site-directed mutagenesis in B31 MI, the low-passage-number, infectious B. burgdorferi s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.4.2139-2150.2002
更新日期:2002-04-01 00:00:00
abstract::To develop targeted gene integration in the periodontal pathogen Actinobacillus actinomycetemcomitans, a ColE1-based, spectinomycin-resistant plasmid containing a segment of the leukotoxin gene was electroporated into strain JP2. In all of the stable spectinomycin-resistant transformants that arose, the plasmid had re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2780-2784.1995
更新日期:1995-07-01 00:00:00
abstract::Earlier work had shown that a collection of anaerobic bacteria, in conjunction with facultative anaerobes, may be implanted into germfree mice, thereby rendering the animals "normal" with respect to a variety of parameters tested. The present experiments indicate that a different collection of anaerobic bacteria, isol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.2.119-126.1972
更新日期:1972-08-01 00:00:00
abstract::Immunoprecipitation of radiolabeled antigens with bovine antisera indicated that many Babesia bigemina and Babesia bovis merozoite proteins contain isolate-common epitopes, while at least 16 B. bigemina and 8 B. bovis proteins contain species-cross-reactive epitopes. One immunogenic, isolate-common, and species-specif...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1658-1660.1988
更新日期:1988-06-01 00:00:00
abstract::Menstrually associated toxic shock syndrome (TSS) is attributed primarily to the effects of staphylococcal exotoxin toxic shock syndrome toxin 1 (TSST-1). A region of the 194-amino-acid toxin spanning residues 115 through 144 constitutes a biologically active site. Several point mutations in the TSST-1 gene in that re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.3.793-799.1993
更新日期:1993-03-01 00:00:00
abstract::Screening of a Mycobacterium tuberculosis genomic DNA library in the lambda gt11 expression vector was carried out by using, as probes, sera from tuberculous patients and murine monoclonal antibody H61.3 recognizing a mycobacterial 35-kilodalton protein present only on the M. tuberculosis complex. The recombinant beta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.245-251.1990
更新日期:1990-01-01 00:00:00
abstract::Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. Here we show that colonic epithelial model HCT116 cells respond to Trypanosoma cruzi infection by secreting defensin α-1, which reduces infection. We also report the early effects of defensin α-1 on invasi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01459-12
更新日期:2013-11-01 00:00:00