Abstract:
:Bacterial strains of Haemophilus species and Streptococcus pneumoniae were examined for synthesis of the enzyme immunoglobulin A1 (IgA1) protease. Of 36 H. influenzae strains examined, 35 produced IgA1 protease; strains included all six capsular types, unencapsulated variants of types b and d, and untypable H. influenzae. Eight Haemophilus strains (non-H. influenzae) were studied, and two produced IgA1 protease. All 10 strains of S. pneumoniae produced IgA1 protease; these strains included 9 different capsular polysaccharide types and 1 untypable strain. Both IgA1 proteases cleaved myeloma IgA1 and secretory IgA but not myeloma IgA2, IgM, or IgG as determined by immunoelectrophoresis. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that both enzymes cleaved IgA1 myeloma sera, but not IgA2, into two fragments. The apparent molecular weight of the cleaved fragments was dependent both on the apparent molecular weight of the cleaved fragments was dependent both on the specific IgA1 protease assayed and the specific IgA1 substrate utilized. It is postulated that both carbohydrate variation between the IgA1 substrates studied and the ability of S. pneumoniae glycosidases to cleave carbohydrates from glycoprotein offer an explanation for the different fragment sizes observed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Male CJdoi
10.1128/IAI.26.1.254-261.1979subject
Has Abstractpub_date
1979-10-01 00:00:00pages
254-61issue
1eissn
0019-9567issn
1098-5522journal_volume
26pub_type
杂志文章abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00
abstract::Fifty Borellia burgdorferi strains isolated from humans and ticks in Europe and the United States were analyzed by multilocus enzyme electrophoresis. Eleven genetic loci were characterized on the basis of the electrophoretic mobilities of their products. Ten loci were polymorphic. The average number of alleles per loc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1677-1683.1992
更新日期:1992-04-01 00:00:00
abstract::Toxicity of lipopolysaccharide (LPS) (endotoxin) is, to a large extent, mediated by the activation of monocytes/macrophages and subsequent release of monokines, such as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha). It is known that LPS binds readily to serum lipoproteins and that LPS-lipoprotein co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.2237-2245.1989
更新日期:1989-07-01 00:00:00
abstract::The ability of a pathogen to evade neutrophil phagocytic killing mechanisms is critically important for dissemination and establishment of a systemic infection. Understanding how pathogens overcome these innate defenses is essential for the development of optimal therapeutic strategies for invasive infections. CpsY is...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00027-12
更新日期:2012-05-01 00:00:00
abstract::Formation of compact colonies of Staphylococcus aureus in serum-soft agar is mainly a result of a reaction between protein A and the Fc-part of immunoglobulin G and not a clumping factor-fibrinogen reaction. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.583-586.1972
更新日期:1972-10-01 00:00:00
abstract::The role of a plasmid in the virulence activity of an enteropathogenic Escherichia coli (EPEC) strain belonging to serotype 0111:NM was examined. EPEC strain B171, which is resistant to chloramphenicol, streptomycin, sulfathiazole, and tetracycline, harbors a 54-megadalton plasmid, pYR111, and exhibits localized adher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2052-2056.1987
更新日期:1987-09-01 00:00:00
abstract::Inbred CBA/J mice were used in developing a defined in vivo model for studying host-parasite relationships in cryptococcosis. Mice were infected either intranasally or intraperitoneally with 10(3) viable Cryptococcus neoformans cells. At weekly intervals over a 92-day period, C. neoformans growth profiles in the lungs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Individual strains of Staphylococcus aureus have different capacities to become internalized by osteoblasts. Here we report that the levels of sigma(B) expressed by S. aureus correlate with the capacity of this bacterium to be internalized by osteoblasts. However, sigma(B) is not essential for internalization and does...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4167-4170.2003
更新日期:2003-07-01 00:00:00
abstract::Porphyromonas gingivalis, a gram-negative anaerobic bacterium associated with active lesions of chronic periodontitis, produces several proteinases which are presumably involved in host colonization, perturbation of the immune system, and tissue destruction. The aims of this study were to investigate the degradation o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.8.4351-4356.2004
更新日期:2004-08-01 00:00:00
abstract::Representative strains of serogroup A Neisseria meningitidis were chosen from all major meningitis epidemics worldwide since 1960 and subjected to analysis for the electrophoretic variation of 15 cytoplasmic allozymes and four outer membrane proteins. The 290 strains defined 84 unique electrophoretic types which were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5267-5282.1992
更新日期:1992-12-01 00:00:00
abstract::The pattern of meningococcal surface structure expression in different microenvironments following bloodstream invasion in vivo is not known. We used immunohistochemistry to determine the expression of capsule, type IV pili, and PorA by meningococci residing in the skin lesions of children with purpura fulminans. All ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5193-5201.2002
更新日期:2002-09-01 00:00:00
abstract::Several group B streptococcal products have been previously found to stimulate human monocytes to produce tumor necrosis factor alpha. In order to identify the receptors involved in these responses, monocytes were stimulated with purified group- or type-specific carbohydrates or lipoteichoic acid in the presence of an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.994-998.2000
更新日期:2000-02-01 00:00:00
abstract::Nineteen of 22 female mice (CF1 strain) inoculated intragastrically with Treponema hyodysenteriae developed cecal and colonic lesions consisting of catarrhal inflammation, edema, and occasional hemorrhage. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.757-760.1979
更新日期:1979-08-01 00:00:00
abstract::Immune cytolysis was measured by release of absorbed radioactive chromium from infected cells that were incubated with antiviral antibody and complement. The presence of virus-specific antigens detected in this manner on the surface of several types of cells infected with Japanese encephalitis (JE) virus did not corre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.2.330-333.1975
更新日期:1975-02-01 00:00:00
abstract::Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots. Protein profiles of the C. pneumoniae isolates appeared to be the same and were distinct from the other Chlamydia spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.93-97.1990
更新日期:1990-01-01 00:00:00
abstract::Borrelia burgdorferi, an agent of Lyme disease, establishes persistent infection in immunocompetent animals and humans. Although the infection in humans can be cleared by antibiotic therapy, persistence in reservoir animals is necessary for the maintenance of the bacterium in the natural reservoir host⇔tick vector inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05122-11
更新日期:2011-08-01 00:00:00
abstract::The urease gene of Staphylococcus saprophyticus CCM883 was cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned DNA sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. Introduction of the cloned genes into a ure...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.2998-3002.1989
更新日期:1989-10-01 00:00:00
abstract::Diverse pathogens have evolved to survive and replicate in the endosomes or phagosomes of the host cells and establish persistent infection. Ehrlichiae are Gram-negative, intracellular bacteria that are transmitted by ticks. Ehrlichiae reside in the endosomes of the host phagocytic or endothelial cells and establish p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02101-14
更新日期:2014-10-01 00:00:00
abstract::Haemophilus ducreyi is resistant to killing by normal serum antibody and complement. We discovered an H. ducreyi outer membrane protein required for expression of serum resistance and termed it DsrA (for "ducreyi serum resistance A"). The dsrA locus was cloned, sequenced, and mutagenized. An isogenic mutant (FX517) of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1608-1619.2000
更新日期:2000-03-01 00:00:00
abstract::Toxoplasma gondii is an obligate intracellular parasite that resides in the cytoplasm of its host in a unique membrane-bound vacuole known as the parasitophorous vacuole (PV). The membrane surrounding the parasite is remodeled by the dense granules, secretory organelles that release an array of proteins into the vacuo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00782-08
更新日期:2008-11-01 00:00:00
abstract::Cutaneous leishmaniasis (CL) caused by Leishmania braziliensis is characterized by a strong Th1 response that leads to skin lesion development. In areas where L. braziliensis transmission is endemic, up to 15% of healthy subjects have tested positive for delayed-type hypersensitivity to soluble leishmania antigen (SLA...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02404-14
更新日期:2015-03-01 00:00:00
abstract::A lectin with strong hemagglutinating activity toward erythrocytes of several animal species was isolated from an 18-h culture supernatant of a diarrheagenic strain, V2, of non-O1 Vibrio cholerae. The hemagglutinin (HA) was purified free of lipopolysaccharide by salt fractionation followed by gel filtration, hydrophob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.11.3698-3705.1990
更新日期:1990-11-01 00:00:00
abstract::Fourteen freshly isolated strains of Streptococcus sanguis were obtained from the dental plaque of five healthy adults. Whole saliva was collected concomitant with the plaque isolates from the five subjects, and a second whole saliva sample was collected 10 weeks later. All possible combinations of the first five sali...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1104-1110.1979
更新日期:1979-12-01 00:00:00
abstract::The recently introduced choleragen-induced rat foot edema model has been employed as a bioassay for evaluating the immunogenicity of three purified preparations containing cholera exo-enterotoxin antigen, choleragen, choleragenoid, and Formalin-treated choleragen (formagen). The results indicated that choleragen evoke...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.1.5.468-473.1970
更新日期:1970-05-01 00:00:00
abstract::The coaggregation-defective (COG-) mutant Actinomyces viscosus T14V(PK455), which is unable to participate in lactose-sensitive adherence, and its COG+ parent were compared to structurally define the mutational loss of cell-associated lectin activity. Immunoelectrophoretic comparisons of crude extracts or isolated fim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.759-765.1983
更新日期:1983-05-01 00:00:00
abstract::In experimental visceral leishmaniasis, in which the tissue macrophage is the target, in vivo responsiveness to conventional chemotherapy (pentavalent antimony [Sb]) requires a T-cell-dependent mechanism. To determine if this mechanism involves gamma interferon (IFN-gamma)-induced activation and/or specific IFN-gamma-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.288-293.2000
更新日期:2000-01-01 00:00:00
abstract::Representative strains of oral streptococci were tested for Fc immunoglobulin G (IgG) reactivity by two different techniques, agglutination of rabbit-IgG sensitized sheep erythrocytes and uptake of human and rabbit 125I-radiolabeled IgG. None of the S. mutans serotypes a through e reacted with the Fc region of either ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.851-855.1981
更新日期:1981-12-01 00:00:00
abstract::Outbred Swiss mice were inoculated intraperitoneally or intravenously with one 90 to 100% lethal dose of Escherichia coli O:18, Proteus mirabilis, or Klebsiella pneumoniae. After carefully timed intervals, aminoglycoside antibiotics were begun at dosages nnd intervals predetermined to constitute optimal therapy. With ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.2.538-557.1979
更新日期:1979-08-01 00:00:00
abstract::Gonococci from opaque colonies have cell wall outer membrane proteins that are lacking from organisms which form transparent colonies. These "colony opacity-associated" proteins are among a group of "minor" proteins that exhibit heat modification of their apparent subunit molecular sizes, are easily extracted by deoxy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.54-64.1980
更新日期:1980-04-01 00:00:00
abstract::The production of delta-toxin is supposed to be responsible for various pathophysiological effects during infection with Staphylococcus aureus. We compared the effects of delta-toxin with the structurally related bee venom toxin melittin on granulocyte functions and inflammatory mediator release. Delta-toxin and melit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1653-1659.1990
更新日期:1990-06-01 00:00:00