Abstract:
:The mechanism(s) of interaction between Mycoplasma pulmonis and eucaryotic cells was studied by adherence to and hemagglutination of erythrocytes. Simple and complex carbohydrates and glycoproteins were unable to inhibit either adherence or hemagglutination, indicating that neither was a lectin activity. Both interactions appeared to be hydrophobic due to their requirement for salt and their sensitivity to temperature. Hemagglutination, but not adherence, was inhibited by both trypsin and glutaraldehyde treatment of the mycoplasma, suggesting that adherence and hemagglutination are qualitatively different. The erythrocyte receptor sites for the two activities were also separable since hemagglutination, but not adherence, required trypsinization of erythrocytes. The hemagglutinin was shown to be an integral mycoplasma component and not a broth contaminant. Once removed, hemagglutinating activity could not be replenished by incubation in serum or broth at 4 degrees C, but could be regenerated during protein synthesis under nonreplicative conditions. Thus, a mycoplasma membrane protein was detected which was capable of interacting with opposing membrane surfaces through hydrophobic interactions. Consequently, a multiphasic model of M. pulmonis-eucaryotic cell interactions was proposed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Minion FC,Cassell GH,Pnini S,Kahane Idoi
10.1128/IAI.44.2.394-400.1984subject
Has Abstractpub_date
1984-05-01 00:00:00pages
394-400issue
2eissn
0019-9567issn
1098-5522journal_volume
44pub_type
杂志文章abstract::Vitamin D(3) (1,25-dihydroxycholecalciferol) induced the phagocyte oxidative burst and intracellular killing of Salmonella enterica serovar Typhimurium in a phosphatidylinositol 3-kinase-dependent manner. The antimicrobial effect was more pronounced for Salmonella SodCI and SodCII mutants, confirming the role of the p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.9.5312-5315.2002
更新日期:2002-09-01 00:00:00
abstract::The bundle-forming pilus (BFP) of enteropathogenic Escherichia coli (EPEC) is an important virulence factor. We examined the role of divergent alleles of bfpA encoding bundlin, the BFP pilin protein, in pilus biogenesis, pilus interactions, and immune responses. We found that the BFP biogenesis machine from an EPEC st...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00009-07
更新日期:2007-10-01 00:00:00
abstract::A Gram-negative, filamentous, rod-shaped bacillus which failed to grow in cell-free media was isolated in apparently pure culture from the bronchial scraping and washing of a laboratory rat suffering from chronic respiratory disease by inoculating embryonated chicken eggs via the allantoic route. None of the embryos d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.2.472-479.1985
更新日期:1985-02-01 00:00:00
abstract::Strains of Corynebacterium ulcerans and Corynebacterium pseudotuberculosis (Corynebacterium ovis) were examined for the production of diphtheria toxin. A majority of C. ulcerans strains (25 of 37) and 1 C. pseudotuberculosis strain (1 of 14) gave a positive Elek test for diphtheria toxin, and for all strains but 1, pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.511-517.1984
更新日期:1984-08-01 00:00:00
abstract::Intravenous injection of rat anti-mouse gamma interferon (IFN-gamma) monoclonal antibody as well as rabbit anti-mouse tumor necrosis factor (TNF) antibody into mice which had received a sublethal infection with Listeria monocytogenes cells resulted in acceleration of listeriosis. Endogenous IFN-gamma seemed to be prod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3331-3337.1989
更新日期:1989-11-01 00:00:00
abstract::The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a signi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7170-7172.2003
更新日期:2003-12-01 00:00:00
abstract::Inoculation of herpes simplex virus on the forehead and/or snout of hairless mice resulted in a significantly lower mortality rate than inoculation of the skin in the lumbosacral area. Latent herpes simplex virus infections were detected in all forehead-inoculated and in 90% of snout-inoculated mice. Phosphonoacetic a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.130-135.1978
更新日期:1978-04-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the human nasopharynx, and yet is also an opportunistic pathogen of the upper and lower respiratory tracts. Host microenvironments influence gene expression patterns, likely critical for NTHi persistence. The host sequesters iron as a mechanism ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01227-12
更新日期:2013-04-01 00:00:00
abstract::Amebic erythrophagocytosis is characteristic of invasive amebiasis, and mutants deficient in erythrocyte ingestion are avirulent. We sought to understand the molecular mechanisms underlying erythrocyte phagocytosis by Entamoeba histolytica. Following adherence to amebae, erythrocytes became round and crenulated, and p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3422-3430.2005
更新日期:2005-06-01 00:00:00
abstract::The lipooligosaccharide (LOS) present in the outer membrane of Haemophilus ducreyi is likely a virulence factor for this sexually transmitted pathogen. An open reading frame in H. ducreyi 35000 was found to encode a predicted protein that had 87% identity with the protein product of the gmhA (isn) gene of Haemophilus ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::We have previously shown that individuals infected with Chlamydia trachomatis can develop a robust antibody response to a chlamydia-secreted protein (designated chlamydial proteasome/protease-like activity factor [CPAF]). We now report that sera collected from these infected individuals neutralized the proteolytic act...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4414-4419.2005
更新日期:2005-07-01 00:00:00
abstract::Infection of resistant or susceptible mice with Trichuris muris provokes mesenteric lymph node responses which are polarized towards Th2 or Th1, respectively. These responses are well documented in the literature. In contrast, little is known about the local responses occurring within the infected intestine. Through m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4025-4033.2005
更新日期:2005-07-01 00:00:00
abstract::An immunosuppressed rat model of Pneumocystis carinii pneumonia is described that utilizes simple, noninvasive intratracheal (i.t.) inoculation of cryopreserved parasites and results in development of severe P. carinii pneumonia within 5 weeks. This is an improvement over the most commonly used models of P. carinii pn...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1589-1597.1992
更新日期:1992-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5379-5385.1999
更新日期:1999-10-01 00:00:00
abstract::Serotypes A and B of the relapsing fever spirochete Borrelia turicatae produce different disease manifestations in infected mice. Whereas serotype B causes more severe arthritis and reaches higher densities in the blood of mice than serotype A, serotype A invades the central nervous system earlier than serotype B duri...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4637-4645.1999
更新日期:1999-09-01 00:00:00
abstract::Borrelia burgdorferi, the Lyme disease pathogen, dramatically alters its protein profile when it is transmitted between ticks and mammals. Several differentially expressed proteins have been shown to be critical for the enzootic cycle of B. burgdorferi. In this study, we demonstrated that expression of the surface lip...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00372-10
更新日期:2010-07-01 00:00:00
abstract::Legionnaires' disease is clinically manifested as severe pneumonia caused by Legionella pneumophila. However, the dendritic cell (DC)-centered immunological framework of the host defense against L. pneumophila has not been fully delineated. For this study, we focused on a potent chemoattractant for lymphocytes, fracta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5350-5357.2005
更新日期:2005-09-01 00:00:00
abstract::Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family E...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.12.5384-5387.1993
更新日期:1993-12-01 00:00:00
abstract::Cell-mediated immunity is pivotal in host resistance to Blastomyces dermatitidis infection. Immunization of mice with the WI-1 adhesin enhances resistance against experimental pulmonary infection but elicits features of a mixed T-helper-cell immune response. Immune mice acquire delayed-type hypersensitivity (DTH) but ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.7172-7174.2000
更新日期:2000-12-01 00:00:00
abstract::Malaria toxin causes hypoglycemia and induction of tumor necrosis factor. Extracts of parasitized erythrocytes which were coeluted and copurified with one of the two subtypes of mammalian insulin-mimetic inositolphosphoglycans similarly induced fibroblast proliferation in the absence of serum. In addition, induction o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3438-3441.1996
更新日期:1996-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1289-1296.2000
更新日期:2000-03-01 00:00:00
abstract::The purpose of these experiments was to study the early response of mouse foot pads to Mycobacterium leprae. To accomplish this, mice were inoculated in both foot pads with large and small numbers of organisms. The animals were sacrificed at intervals from 2 hr to 27 days after inoculation. The microscopical results, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.1.76-85.1973
更新日期:1973-01-01 00:00:00
abstract::Molecular approaches to the rapid analysis of the serotyping antigens of Neisseria meningitidis, the class 2 and 3 outer membrane proteins (OMPs), were developed, evaluated, and used to study 12 antigenic variants of these proteins. A primer set for the polymerase chain reaction (PCR) amplification of the genes encodi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3620-3629.1992
更新日期:1992-09-01 00:00:00
abstract::Transgenic tobacco plants stably expressing recombinant FaeG, which is the major subunit and adhesin of K88ad fimbriae, were obtained. Analysis of sera from immunized mice indicates that in mice, the immunogenicity induced by plant-derived FaeG protein is comparable to that generated with traditional approaches. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5436-5439.2003
更新日期:2003-09-01 00:00:00
abstract::Chromosomal DNA from a uropathogenic strain of Escherichia coli was partially digested with the restriction enzyme EcoRI. The partial digests were ligated into a cosmid containing an ampicillin-resistant determinant and packaged into lambda phage particles. An ampicillin-resistant transductant of E. coli HB101 was fou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.739-744.1982
更新日期:1982-11-01 00:00:00
abstract::The distribution of dengue-2 antigens was studied in infected monkey kidney cells (LLC MK2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. This procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1699-1709.1976
更新日期:1976-06-01 00:00:00
abstract::The possibility that gonococcal energy might play a role in the interaction of Neisseria gonorrhoeae with both phagocytic and nonphagocytic cells was examined. Respiratory chain inhibitors including KCN and amobarbital resulted in reduction in gonococcal association with human neutrophils. Similar results were seen wi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.785-790.1989
更新日期:1989-03-01 00:00:00
abstract::Antiprotein monoclonal antibodies derived from mice inoculated with Rickettsia rickettsii heated at 56 degrees C for 15 min are of two types: one is type specific for epitopes denatured by moderate temperatures, and the other is specific for epitopes resistant to 100 degrees C for 5 min. The heat-resistant epitopes ar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.825-827.1987
更新日期:1987-03-01 00:00:00
abstract::The mucosal and systemic immune responses after primary and booster immunizations with two attenuated live oral vaccine strains derived from a noninvasive (Vibrio cholerae) and an invasive (Salmonella typhi) enteric pathogen were comparatively evaluated. Vaccination with S. typhi Ty21a elicited antibody-secreting cell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3680-3685.1999
更新日期:1999-07-01 00:00:00
abstract::A preparation procedure for scanning electron microscopy was developed by which gononcoccal colonies can be studied directly on the agar surface. After glutaraldehyde fixation directly in petri dishes, small agar pieces were cut out and dehydrated stepwise in increasing concentrations of ethanol. The blocks were there...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.3.630-637.1975
更新日期:1975-09-01 00:00:00