Abstract:
:Glucose metabolism and respiration of Candida albicans were compared under conditions which permitted either maximal filamentous or maximal yeast growth. Changes in metabolism were monitored by comparing the quantities of ethanol produced, CO2 evolved, and oxygen consumed. Filamenting cultures produced more ethanol and less CO2 than yeasts, with oxygen consumption in the former concomitantly slower than that of the latter. Studies involving cofactors and inhibitors associated with electron transport imply that a transfer of electrons away from flavoprotein is required for maintenance of yeast morphology. Conditions consistent with a buildup of reduced flavoprotein, however, favored filament formation. These changes were expressed metabolically as a shift from an aerobic to a fermentative metabolism. The results presented are consistent with hypotheses correlating filament production with changes in carbohydrate metabolism and an interruption of electron transfer within the cell.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Land GA,McDonald WC,Stjernholm RL,Friedman Ldoi
10.1128/IAI.12.1.119-127.1975keywords:
subject
Has Abstractpub_date
1975-07-01 00:00:00pages
119-27issue
1eissn
0019-9567issn
1098-5522journal_volume
12pub_type
杂志文章abstract::Studies of mice suggest that pneumococcal proteins, including PspA, pneumolysin, PsaA, and CbpA, are promising vaccine candidates. To determine whether these proteins are good mucosal immunogens in humans, adenoidal lymphocytes from 20 children who had adenoidectomies were isolated and tested by ELISpot for antigen-sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5363-5369.2002
更新日期:2002-10-01 00:00:00
abstract::An unusual 120-kDa alkaline peptidase contained in a trypomastigote soluble fraction (TSF) of Trypanosoma cruzi is associated with the induction of repetitive Ca2+ transients and subsequent invasion by the parasite of a number of mammalian cell lines, including tissue culture L6E2 myoblasts (B. A. Burleigh and N. W. A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1770-1777.1996
更新日期:1996-05-01 00:00:00
abstract::A method was developed to study the adhesion of Streptococcus pneumoniae to human pharyngeal epithelial cells. Epithelial cells from healthy persons, pneumococcal strains from patients with otitis media, meningitis, or septicemia, and pneumococcal cells from the nasopharynx of healthy carriers were used. Adhesion was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.311-317.1981
更新日期:1981-04-01 00:00:00
abstract::The amino acid sequence of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli 18D has been revised. Amino acids originally assigned to positions 11 and 18, i.e., Tyr and Asn, respectively, were found to occupy positions 18 and 11, respectively. Thus all heat-stable enterotoxins composed of 18 amino a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.834-836.1985
更新日期:1985-03-01 00:00:00
abstract::To characterize the roles of Porphyromonas gingivalis and its components in disease processes, we investigated the cytokine profiles induced by live P. gingivalis, its lipopolysaccharide (LPS), and its major fimbrial protein, fimbrillin (FimA). A cytokine antibody array revealed that human monocyte-derived macrophages...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1204-1214.2006
更新日期:2006-02-01 00:00:00
abstract::Sonicated preparations of capsulated Haemophilus influenzae type b, two of its spontaneous mutants, one containing patches of capsules (class I variant) and the other noncapsulated (class II variant), and a noncapsulated strain of H. influenzae type d were analyzed by crossed-immunoelectrophoresis using unadsorbed ant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1733-1742.1976
更新日期:1976-06-01 00:00:00
abstract::Virulent strains of Pseudomonas aeruginosa are either of a nonmucoid, lipopolysaccharide (LPS)-smooth or mucoid, LPS-rough phenotype, and immunity to these different variants is efficiently mediated by antibodies specific to O antigens or mucoid exopolysaccharide (also called alginate), respectively. In addition to O ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.21-26.1995
更新日期:1995-01-01 00:00:00
abstract::Tuberculosis is one of the most important infectious diseases worldwide. The susceptibility to this disease depends to a great extent on the innate immune response against mycobacteria. Host defense peptides (HDP) are one of the first barriers to counteract infection. Cathelicidin (LL-37) is an HDP that has many immun...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00936-15
更新日期:2015-12-01 00:00:00
abstract::Neutrophil extracellular traps (NETs) are produced by neutrophils as an innate immune defense mechanism to trap and kill microbial pathogens. NETs are comprised of ejected chromatin that forms a lattice structure enmeshed with numerous antimicrobial proteins. In addition to forming the structural backbone of NETs, ext...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00403-18
更新日期:2018-08-22 00:00:00
abstract::Cytomegalovirus (CMV) infection is common after renal transplantation, and cytomegaloviruria occurs in about two-thirds of the recipients. These observations suggest that the allografts may be a site of latent infection with CMV and its reactivation may be the source of viruria. To investigate this possibility, 130 ki...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.699-703.1978
更新日期:1978-02-01 00:00:00
abstract::The effector mechanism, which operates against challenge parasites in the lungs of C57BL/6 mice vaccinated once with irradiated cercariae of Schistosoma mansoni, is mediated by CD4+ T helper lymphocytes. However, adoptive transfer of immunity from vaccinated donors to naive recipients by using sensitized T cells has n...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.42-48.1997
更新日期:1997-01-01 00:00:00
abstract::Our previous studies demonstrated that Mycobacterium bovis bacillus Calmette-Guérin (BCG) can directly interact with human NK cells and induce the proliferation, gamma interferon production, and cytotoxic activity of such cells without the need for accessory cells. Thus, the aim of the present study was to identify th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00870-07
更新日期:2008-04-01 00:00:00
abstract::Purified group A streptococcal peptidoglycan-polysaccharide (PG-PS) fragments were either de-O-acylated, or acetylated and then de-O-acylated to yield N-acetylated PG-PS. Native PG-PS was poorly degraded, N-acetylated PG-PS was extensively degraded, and de-O-acylated PG-PS was only slightly degraded by hen egg white l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.1.16-23.1987
更新日期:1987-01-01 00:00:00
abstract::The porcine immunoglobulins M (IgM), A (IgA), and G (IgG) were isolated and purified and some of the properties of the porcine milk IgA were examined. Monospecific antisera which were prepared against these immunoglobulins in rabbits were then used to absorb a particular class of immunoglobulin from sow serum, colostr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.600-609.1972
更新日期:1972-10-01 00:00:00
abstract::Capsular glucuronoxylomannans (GXM) of Cryptococcus neoformans var. neoformans isolates from patients with recurrent cryptococcal meningitis were analyzed by 1H nuclear magnetic resonance spectroscopy and for reactivity with factor sera (Iatron, Tokyo, Japan). For each patient the initial and relapse isolates had prev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1899-1905.1995
更新日期:1995-05-01 00:00:00
abstract::Improvement of antibody responses to polysaccharides through their linkage to proteins is thought to be mediated by protein-specific T helper (Th) cells. To investigate whether the carrier protein of a conjugate could be substituted by a Th epitope, Streptococcus pneumoniae type 17F polysaccharide (PS) was bromoacetyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.961-968.1995
更新日期:1995-03-01 00:00:00
abstract::Brucella species can cause brucellosis, a zoonotic disease that causes serious livestock economic losses and represents a public health threat. The mechanism of virulence of Brucella spp. is not yet fully understood. Therefore, it is crucial to identify new molecules that serve as virulence factors to better understan...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02790-14
更新日期:2015-04-01 00:00:00
abstract::Severe combined immunodeficient beige mice were inoculated orally and intraperitoneally with a bovine strain of Mycobacterium paratuberculosis to explore their potential as laboratory animal models in the study of paratuberculosis (Johne's disease). Control animals were similarly inoculated with heat-killed M. paratub...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4074-4079.1992
更新日期:1992-10-01 00:00:00
abstract::Human granulocytic anaplasmosis (HGA) is caused by the obligate intracellular bacterium Anaplasma phagocytophilum. The critical role of gamma interferon (IFN-gamma) for induction of severe inflammatory histopathology, even in the absence of a significant bacterial load, was previously demonstrated in a murine model of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00098-07
更新日期:2007-06-01 00:00:00
abstract::Little data are available regarding L-form phagocyte interactions. Due to difficulty with more conventional methods, a serum-free technique of surface phagocytosis employing cellulose ester micropore filters, fluorochrome staining, and reflecting fluorescence microscopy was developed. Intracellular localization of L-f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.6.976-981.1972
更新日期:1972-06-01 00:00:00
abstract::Platelets aggregate in response to an adhesin and the platelet aggregation-associated protein (PAAP) expressed on the cell surfaces of certain strains of Streptococcus sanguis. We sought to identify the corresponding PAAP receptor and accessory adhesin binding sites on platelets. Since the adhesion(s) of S. sanguis fo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3628-3633.1995
更新日期:1995-09-01 00:00:00
abstract::The method of Garra and Baygorria, for localized antibody production, has been adapted for obtaining high-titered and monospecific antibodies against the murine toxin of Yersinia pestis. A hollow, perforated plastic golf ball surgically implanted under the skin of rabbits induced the formation of a granuloma. When the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.3.458-463.1974
更新日期:1974-09-01 00:00:00
abstract::Antimicrobial peptides, such as defensins or cathelicidins, are effector substances of the innate immune system and are thought to have antimicrobial properties that contribute to host defense. The evidence that vertebrate antimicrobial peptides contribute to innate immunity in vivo is based on their expression patter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6084-6089.1999
更新日期:1999-11-01 00:00:00
abstract::We have examined the ability of peptidoglycan (PepG) and lipoteichoic acid (LTA) isolated from Staphylococcus aureus to induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and IL-10 in whole human blood and identified the cellular origins of these cytokines. Both PepG and LTA induced t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.3965-3970.2000
更新日期:2000-07-01 00:00:00
abstract::We investigated the ability of using monoclonal antibodies (MAbs) against anthrax protective antigen (PA), an anthrax exotoxin component, to modulate exotoxin cytotoxic activity on target macrophage cell lines. Anthrax PA plays a critical role in the pathogenesis of Bacillus anthracis infection. PA is the cell-binding...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3276-3283.2004
更新日期:2004-06-01 00:00:00
abstract::The 25-kb linear plasmid lp25 and one of the 28-kb linear plasmids (lp28-1) are required for experimental infection in Borrelia burgdorferi, the etiologic agent of Lyme disease. The loss of these plasmids either eliminates infectivity (lp25) or significantly increases the 50% infective dose during a 2-week infection p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4608-4613.2003
更新日期:2003-08-01 00:00:00
abstract::Chlamydia trachomatis infections can lead to severe chronic complications, including trachoma, ectopic pregnancy, and infertility. The only effective approach to disease control is vaccination. The goal of this work was to identify new potential vaccine candidates through a proteomics approach. We constructed a protei...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00626-10
更新日期:2011-01-01 00:00:00
abstract::Mice infected with various strains of Salmonella enteritidis and S. typhimurium were found to be more sensitive to the cell wall lipopolysaccharide (LPS) extracted from certain strains of Salmonella than noninfected mice. This hypersensitivity was induced by those smooth or rough strains which possessed a polysacchari...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.519-524.1971
更新日期:1971-11-01 00:00:00
abstract::Treatment with nontoxic monophosphoryl lipid A (MPL), which was derived from a polysaccharide-deficient, heptoseless Re mutant of Salmonella typhimurium, was found to inactivate suppressor T-cell activity, as evidenced by a decrease in the degree of low-dose immunological paralysis expressed and an increase in the mag...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1076-1083.1988
更新日期:1988-05-01 00:00:00
abstract::Host-driven macrophage apoptosis contributes to innate immunity during bacterial infection. Neisseria meningitidis inhibits apoptosis in a variety of cells, but its impact on macrophage apoptosis is unknown. We demonstrate that N. meningitidis prevents macrophage apoptosis via genes encoding nitric oxide detoxificatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.729-733.2006
更新日期:2006-01-01 00:00:00