Mechanism of hepatic parenchyma-specific contrast of microbubble-based contrast agent for ultrasonography: microscopic studies in rat liver.

Abstract:

OBJECTIVE:The objective of this study was to elucidate the mechanism of hepatic parenchyma-specific contrast of Sonazoid (microbubble contrast agent) using microscopic techniques. MATERIALS AND METHODS:Sonazoid was intravenously injected into rats to investigate the microbubble dynamics and distribution within hepatic microcirculation in exteriorized liver using intravital microscopy and to observe dose dependency of ultrasound hepatic contrast effect. In vitro and in vivo uptake of microbubbles by Kupffer cells was examined using confocal laser scanning microscopy. RESULTS:Intravital observation demonstrated freely flowing microbubbles in the sinusoid and some microbubbles co-localized with Kupffer cells. The microbubbles internalized in Kupffer cells were identified with reflected light by confocal laser scanning microscopy. The percentage of Kupffer cells taking up microbubbles was about 1% at clinical dose at which the homogeneous hepatic contrast was observed. CONCLUSIONS:The hepatic parenchyma-specific contrast by Sonazoid is due to distribution of the microbubbles in Kupffer cells.

journal_name

Invest Radiol

journal_title

Investigative radiology

authors

Watanabe R,Matsumura M,Munemasa T,Fujimaki M,Suematsu M

doi

10.1097/RLI.0b013e31805f2682

subject

Has Abstract

pub_date

2007-09-01 00:00:00

pages

643-51

issue

9

eissn

0020-9996

issn

1536-0210

pii

00004424-200709000-00006

journal_volume

42

pub_type

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