Abstract:
OBJECTIVES:Contrast-enhanced ultrasound imaging is increasingly being used in the clinic for assessment of tissue vascularity. The purpose of our study was to evaluate the effect of different contrast administration parameters on the in vivo ultrasound imaging signal in tumor-bearing mice using a maximum intensity persistence (MIP) algorithm and to evaluate the reliability of in vivo MIP imaging in assessing tumor vascularity. The potential of in vivo MIP imaging for monitoring tumor vascularity during antiangiogenic cancer treatment was further evaluated. MATERIALS AND METHODS:In intraindividual experiments, varying contrast microbubble concentrations (5 × 10⁵, 5 × 10⁶, 5 × 10⁷, 5 × 10⁸ microbubbles in 100 μL saline) and contrast injection rates (0.6, 1.2, and 2.4 mL/min) in subcutaneous tumor-bearing mice were applied and their effects on in vivo contrast-enhanced ultrasound MIP imaging plateau values were obtained using a dedicated small animal ultrasound imaging system (40 MHz). Reliability of MIP ultrasound imaging was tested following 2 injections of the same microbubble concentration (5 × 10⁷ microbubbles at 1.2 mL/min) in the same tumors. In mice with subcutaneous human colon cancer xenografts, longitudinal contrast-enhanced ultrasound MIP imaging plateau values (baseline and at 48 hours) were compared between mice with and without antiangiogenic treatment (antivascular endothelial growth factor antibody). Ex vivo CD31 immunostaining of tumor tissue was used to correlate in vivo MIP imaging plateau values with microvessel density analysis. RESULTS:In vivo MIP imaging plateau values correlated significantly (P = 0.001) with contrast microbubble doses. At 3 different injection rates of 0.6, 1.2, and 2.4 mL/min, MIP imaging plateau values did not change significantly (P = 0.61). Following 2 injections with the same microbubble dose and injection rate, MIP imaging plateau values were obtained with high reliability with an intraclass correlation coefficient of 0.82 (95% confidence interval: 0.64, 0.94). In addition, in vivo MIP imaging plateau values significantly correlated (P = 0.01; R² = 0.77) with ex vivo microvessel density analysis. Tumor volumes in treated and nontreated mice did not change significantly (P = 0.22) within 48 hours. In contrast, the change of in vivo MIP imaging plateau values from baseline to 48 hours was significantly different (P = 0.01) in treated versus nontreated mice. CONCLUSIONS:Contrast-enhanced ultrasound MIP imaging allows reliable assessment of tumor vascularity and monitoring of antiangiogenic cancer therapy in vivo, provided that a constant microbubble dose is administered.
journal_name
Invest Radioljournal_title
Investigative radiologyauthors
Pysz MA,Foygel K,Panje CM,Needles A,Tian L,Willmann JKdoi
10.1097/RLI.0b013e3181f9202dsubject
Has Abstractpub_date
2011-03-01 00:00:00pages
187-95issue
3eissn
0020-9996issn
1536-0210journal_volume
46pub_type
杂志文章abstract:RATIONALE AND OBJECTIVES:Interstitial laser photocoagulation (ILP) causes localized tissue necrosis. This study was performed to determine 1) whether the changes seen on computed tomography (CT) correspond to the necrosis pathologically, and 2) which CT technique best shows the necrosis. METHODS:Eighteen Wistar rats h...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-199312000-00014
更新日期:1993-12-01 00:00:00
abstract:OBJECTIVES:The objective of this study was to evaluate the use of dielectric pads for improving high spatial resolution imaging of the inner ear at 7 T. MATERIALS AND METHODS:Two sets of dielectric pads were designed using electromagnetic simulations and implemented using a deuterated suspension of barium titanate. Th...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/RLI.0000000000000026
更新日期:2014-05-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:In contrast-enhanced dual-energy subtraction imaging 2 images acquired postcontrast media administration at different energies are subtracted to highlight structures hidden in the absence of contrast media. X-ray spectra of the newly developed digital full-field mammography units (GE Senographe...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/01.RLI.0000077124.24140.bd
更新日期:2003-09-01 00:00:00
abstract:OBJECTIVE:Cardiac magnetic resonance imaging (MRI) at 3.0 T has recently become available and potentially provides a significant improvement of tissue contrast in T1-weighted imaging techniques relying on Gd-based contrast enhancement. Imaging at high-field strength may be especially advantageous for methods relying on...
journal_title:Investigative radiology
pub_type: 临床试验,杂志文章
doi:10.1097/01.rli.0000233321.82194.09
更新日期:2006-09-01 00:00:00
abstract::The effect of intravenous urography (IVU) on the excretion rate and precipitation of Tamm-Horsfall glycoprotein (THG) has been evaluated in 19 patients. Urine samples were collected immediately before the administration of contrast medium (U1) and then during the period of the excretion urogram (U2). The urinary THG: ...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-198501000-00014
更新日期:1985-01-01 00:00:00
abstract:PURPOSE:We sought to develop a robotic system for computed tomography (CT)-guided biopsy to validate the feasibility, accuracy, and efficacy of the system using phantom tests. MATERIALS AND METHODS:Ten peas (mean diameter 9.9+/-0.4 mm) embedded within a gel phantom were selected for biopsy. Once the best access was de...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/01.rli.0000155285.05672.cf
更新日期:2005-04-01 00:00:00
abstract::Capacity-limited elimination of sodium iopanoate and a reproducible apparent transport maximum (Tm) were demonstrated in nonoperated dogs having an intact enterohepatic circulation and normal endogenous bile salt pool. Using a multiple infusion technique, estimation of apparent Tm of iopanoate in the liver was possibl...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-197601000-00007
更新日期:1976-01-01 00:00:00
abstract::Using real-time ultrasound, the motions of the tongue, hyoid bone, and larynx were monitored and timed during swallowing in ten normal subjects. A well-defined propulsive wave of the tongue was observed during swallowing a 5-cc water bolus. The body of the hyoid bone was identified on the ultrasound scan as a high ech...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-198403000-00003
更新日期:1984-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To evaluate different vein graft-coated stent systems in the endovascular treatment of experimental arteriovenous fistulae (AVF) in a canine model. METHODS:Bilateral carotid-to-external jugular vein AVF were created. Two balloon-expandable tantalum stents (Strecker stent), two self-expanding n...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-199901000-00004
更新日期:1999-01-01 00:00:00
abstract::Observer performance in radiologic interpretation has traditionally been based upon Receiver Operating Characteristic (ROC) analysis. A statistical method that evaluates the reproducibility or consistency of radiologic interpretation is presented. Since this method is not based upon pathologic verification, it cannot ...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-198411000-00024
更新日期:1984-11-01 00:00:00
abstract:UNLABELLED:Szapiro D, Ghaye B, Willems V, et al. Evaluation of CT time-density curves of lower-limb veins. Invest Radiol 2001;36:164-169. RATIONALE AND OBJECTIVES:To evaluate time-density curves of the lower-limb veins for optimization of CT venography. METHODS:Fifty patients referred for chest CT were randomized int...
journal_title:Investigative radiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004424-200103000-00005
更新日期:2001-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The basic characteristics of BR1, a novel echo contrast agent based on stabilized sulfur hexafluoride (SF6) microbubbles have been evaluated. METHODS:The authors determined the physicochemical properties (bubble concentration, bubble size distribution, resistance to pressure, and stability) an...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-199508000-00001
更新日期:1995-08-01 00:00:00
abstract:OBJECTIVES:Ferumoxytol, an intravenous iron supplement, can be used in off-label mode as a contrast agent in magnetic resonance imaging. The aim of this study was to assess whether ferumoxytol can be used as a marker of inflammation in animal models of acute and chronic inflammatory kidney diseases. MATERIAL AND METHO...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/RLI.0000000000000209
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:To investigate three-echo T2*-corrected Dixon magnetic resonance imaging (MRI) for noninvasively estimating hepatic fat content (HFC) compared with biopsy. MATERIALS AND METHODS:One hundred patients (50 men, 50 women; mean age, 57.7±14.2 years) underwent clinically indicated liver core biopsy (102 valid tiss...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/RLI.0b013e31822b124c
更新日期:2011-12-01 00:00:00
abstract::The retropsoas recess, a previously undescribed normal anatomic variant, is reported. In most individuals, the space posterior to the belly of the psoas muscle contains fat within the posterior pararenal space. However, a retrospective review of 250 consecutive CT examinations of the abdomen revealed 11 cases (4.4%) i...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-198707000-00004
更新日期:1987-07-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the feasibility of a prototype volume-interpolated breath-hold examination (VIBE) sequence using compressed sensing (VIBECS) for rapid multiphase arterial magnetic resonance imaging (MRI) at different temporal resolution during free-breathing in comparison with a convention...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/RLI.0000000000000459
更新日期:2018-10-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To present the results of two studies conducted to evaluate the pharmacokinetics and safety of iomeprol in healthy volunteers and in patients with various degrees of renal impairment. METHODS:In these two open-label, single-dose, phase I studies, a 50-mL dose of iomeprol 400 was administered i...
journal_title:Investigative radiology
pub_type: 临床试验,杂志文章
doi:10.1097/00004424-200106000-00002
更新日期:2001-06-01 00:00:00
abstract:OBJECTIVES AND RATIONALE:Negative enhancement of implanted liver tumors has been achieved in preclinical studies on manganese dipyridoxal-diphosphate (Mn-DPDP), a new hepatobiliary specific contrast agent for magnetic resonance imaging (MRI). The authors investigated the effects of Mn-DPDP on primary liver cancer and i...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-199306000-00011
更新日期:1993-06-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:Emulsions of perfluorocarbons (PFCs) have been tested as blood substitutes. However, evidence exists that there is long-term retention of some PFCs by the organs of the reticuloendothelial system (RES). The authors investigate organ retention of the blood substitute component, perfluorotripropy...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-199208000-00012
更新日期:1992-08-01 00:00:00
abstract::We measured the amounts of three types of radiographic contrast media (RCM) that could be entrapped in liposomes prepared by the dehydration-rehydration vesicle (DRV) technique. To make DRVs, one initially makes water-containing, small unilamellar vesicles, adds contrast media and lyophilizes the mixture. Upon rehydra...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-198802000-00009
更新日期:1988-02-01 00:00:00
abstract::The Point Spread Function (PSF) in NMR imaging is the result of both the line broadening due to magnet field inhomogeneity and the intrinsic spectrum of the nucleus at resonance. In the case of proton imaging, the line broadening dominates the small chemical shifts and the spectral lines are not resolved. This is not ...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-198503000-00016
更新日期:1985-03-01 00:00:00
abstract:RATIONALE AND OBJECTIVE:The purpose of this study was to investigate the feasibility, efficacy, and safety of ultrasound-guided percutaneous cryotherapy of stage T1 breast cancers. MATERIALS AND METHODS:Thirty patients with biopsy-confirmed breast cancers with tumor diameters of 15 mm or smaller (range, 5-15 mm; media...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/01.rli.0000166935.56971.ff
更新日期:2005-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:To assess the influence of injection rates and volumes on the arrival time of contrast material (CM) in the common carotid artery, the jugular vein and the resulting arterial-venous transit time. Additionally the relationship between injection parameters and the extent of a CM plateau was evalu...
journal_title:Investigative radiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.rli.0000105040.40925.c8
更新日期:2004-02-01 00:00:00
abstract::Intravenous or intra-aortic injections of meglumine iothalamate in dogs indicate a slightly different tissue distribution and excretory pattern of the meglumine cation and the iothalamate anion. Differences in serum and urinary concentrations of these ions suggest some cellular penetration of the meglumine anion. In a...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-197507000-00004
更新日期:1975-07-01 00:00:00
abstract:RATIONALE AND OBJECTIVES:The authors determine the relative effect of soft-tissue compartments, body fat (percent [%Bfat] and weight [Bfat kg]) and fat-free mass (FFM kg), on measurements of ultrasound bone velocity (UBV m/second). METHODS:The authors measured UBV in proximal phalanxes and body fat and fat-free mass b...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-199710000-00005
更新日期:1997-10-01 00:00:00
abstract::We have examined the metabolic behavior of several triiodobenzoic acid derivatives because of the observed mutagenicity and cytotoxicity of 3-amino-5-acetamido-2,4,6-triiodobenzoate (I) and 3,5-diamino-2,4,6-triiodobenzoate (II). Measurements were facilitated using iodine-131 (131I)-labeled compounds. Drug retention i...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::The authors report a randomized, double-blind prospective trial comparing the effects of nonionic with ionic low-osmolar contrast media on erythrocyte aggregation. The study group comprised 40 consecutive patients with pathology necessitating selective angiography. Patients were examined using either ioxaglate 320, io...
journal_title:Investigative radiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004424-199108000-00009
更新日期:1991-08-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine if voxel-based histogram analysis of intravoxel incoherent motion imaging (IVIM) parameters can differentiate various subtypes of renal tumors, including benign and malignant lesions. SUBJECTS AND METHODS:A total of 44 patients with renal tumors who underwent surgery a...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/RLI.0000000000000111
更新日期:2015-03-01 00:00:00
abstract::Recent experimental data underlies the role of hypoxic tubular injury in the pathophysiology of radiocontrast nephropathy. Although systemic transient hypoxemia, increased blood viscosity, and a leftward shift of the oxygen-hemoglobin dissociation curve may all contribute to intrarenal hypoxia, imbalance between oxyge...
journal_title:Investigative radiology
pub_type: 杂志文章,评审
doi:10.1097/00004424-199911000-00004
更新日期:1999-11-01 00:00:00
abstract::Twenty-six patients who had received 43 grafts were studied before and after coronary artery vein bypass surgery. Cine ventriculograms taken in the right anterior oblique projection were analyzed and regional ejection fractions calculated. Single and multiple grafts were considered separately as were thrombosed and pa...
journal_title:Investigative radiology
pub_type: 杂志文章
doi:10.1097/00004424-197705000-00001
更新日期:1977-05-01 00:00:00