Deletion of the cruciform binding domain in CBP/14-3-3 displays reduced origin binding and initiation of DNA replication in budding yeast.

abstract：BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...

journal_title：BMC molecular biology

authors： Ordinario EC,Yabuki M,Handa P,Cummings WJ,Maizels N

更新日期：2009-10-28 00:00:00

abstract：BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...

journal_title：BMC molecular biology

authors： Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo P

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...

journal_title：BMC molecular biology

authors： Skern R,Frost P,Nilsen F

更新日期：2005-04-26 00:00:00

abstract：BACKGROUND:Physical exercise can improve brain function by altering brain gene expression. The expression mechanisms underlying the brain's response to exercise still remain unknown. miRNAs as vital regulators of gene expression may be involved in regulation of brain genes in response to exercise. However, as yet, very...

journal_title：BMC molecular biology

authors： Zhao Y,Zhang A,Wang Y,Hu S,Zhang R,Qian S

更新日期：2019-01-15 00:00:00

abstract：BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...

journal_title：BMC molecular biology

authors： Dedman AM,Majeed Y,Tumova S,Zeng F,Kumar B,Munsch C,Bateson AN,Wittmann J,Jäck HM,Porter KE,Beech DJ

更新日期：2011-07-12 00:00:00

abstract：BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...

journal_title：BMC molecular biology

authors： Gervais AL,Gaudreau L

更新日期：2009-03-04 00:00:00

abstract：BACKGROUND:Invitrogen Gateway technology exploits the integrase/att site-specific recombination system for directional cloning of PCR products and the subsequent subcloning into destination vectors. One or three DNA segments can be cloned using Gateway or MultiSite Gateway respectively. A vast number of single-site Gat...

journal_title：BMC molecular biology

authors： Magnani E,Bartling L,Hake S

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...

journal_title：BMC molecular biology

authors： Liddiard K,Welch JS,Lozach J,Heinz S,Glass CK,Greaves DR

更新日期：2006-11-29 00:00:00

abstract：BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...

journal_title：BMC molecular biology

authors： Mickle KL,Ramanathan S,Rosebrock A,Oliva A,Chaudari A,Yompakdee C,Scott D,Leatherwood J,Huberman JA

更新日期：2007-12-19 00:00:00

abstract：BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...

journal_title：BMC molecular biology

authors： Di Lisi R,Picard A,Ausoni S,Schiaffino S

更新日期：2007-09-17 00:00:00

abstract：BACKGROUND:cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol described by Hubank and Schatz. RESULTS:In the post-hybridization mix, the 5'-ends of homoduplexes of inte...

journal_title：BMC molecular biology

authors： Kuvbachieva AA,Goffinet AM

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:Previous studies suggest that human RAD9 (hRad9), encoding a DNA damage checkpoint molecule, which is frequently amplified in epithelial tumor cells of breast, lung, head and neck cancer, participates in regulation of the tumor suppressor p53-dependent transactivation of pro-survival P21WAF1. This study exam...

journal_title：BMC molecular biology

authors： Ishikawa K,Ishii H,Murakumo Y,Mimori K,Kobayashi M,Yamamoto K,Mori M,Nishino H,Furukawa Y,Ichimura K

更新日期：2007-05-21 00:00:00

abstract：BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...

journal_title：BMC molecular biology

authors： Rutledge RG,Stewart D

更新日期：2008-10-30 00:00:00

abstract：BACKGROUND:Modulation of chromatin structure has emerged as a critical molecular device to control gene expression. Histones undergo different post-translational modifications that increase chromatin accessibility to a number of regulatory factors. Among them, histone ubiquitination appears relevant in nuclear processe...

journal_title：BMC molecular biology

authors： Pinato S,Scandiuzzi C,Arnaudo N,Citterio E,Gaudino G,Penengo L

更新日期：2009-06-05 00:00:00

abstract：BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...

journal_title：BMC molecular biology

authors： Zhang SC,MacDonald KA,Baguma-Nibasheka M,Geldenhuys L,Casson AG,Murphy PR

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...

journal_title：BMC molecular biology

authors： Ozber N,Baris I,Tatlici G,Gur I,Kilinc S,Unal EB,Kavakli IH

更新日期：2010-09-14 00:00:00

abstract：BACKGROUND:Sequence periodicity with a period close to the DNA helical repeat is a very basic genomic property. This genomic feature was demonstrated for many prokaryotic genomes. The Escherichia coli sequences display the period close to 11 base pairs. RESULTS:Here we demonstrate that practically only ApA/TpT dinucle...

journal_title：BMC molecular biology

authors： Hosid S,Trifonov EN,Bolshoy A

更新日期：2004-08-26 00:00:00

abstract：BACKGROUND:Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference ("housekeeping") gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping ...

journal_title：BMC molecular biology

authors： McCurley AT,Callard GV

更新日期：2008-11-12 00:00:00

abstract：BACKGROUND:Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regula...

journal_title：BMC molecular biology

authors： Calvo E,Grzenda A,Lomberk G,Mathison A,Iovanna J,Urrutia R

更新日期：2014-05-25 00:00:00

abstract：BACKGROUND:Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purificatio...

journal_title：BMC molecular biology

authors： Voss C,Schmitt B,Werner-Simon S,Lutz C,Simon W,Anderl J

更新日期：2014-04-03 00:00:00

abstract：BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...

journal_title：BMC molecular biology

authors： Thiel G,Al Sarraj J,Stefano L

更新日期：2005-01-19 00:00:00

abstract：BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

journal_title：BMC molecular biology

authors： Fleming VA,Geng C,Ladd AN,Lou H

更新日期：2012-12-10 00:00:00

abstract：BACKGROUND:The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably the most abundant class of enhancers contains A/U-rich sequences. We have tested the influence of SD sequence length and the presence of enhancers on the efficiency o...

journal_title：BMC molecular biology

authors： Vimberg V,Tats A,Remm M,Tenson T

更新日期：2007-10-31 00:00:00

abstract：BACKGROUND:Cyclic AMP-dependent protein kinase (PKA) plays a central role in regulation of energy metabolism. Upon stimulation of testicular Sertoli cells by follicle stimulating hormone (FSH), glycolysis is activated to increase the production of nutrients for the germ cells, and a new regulatory subunit of cAMP-depen...

journal_title：BMC molecular biology

authors： Dahle MK,Taskén K,Taskén KA

更新日期：2002-06-21 00:00:00

abstract：BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...

journal_title：BMC molecular biology

authors： Jin H,Vacic V,Girke T,Lonardi S,Zhu JK

更新日期：2008-01-14 00:00:00

abstract：BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...

journal_title：BMC molecular biology

authors： Kivistik PA,Kivi R,Kivisaar M,Hõrak R

更新日期：2009-05-16 00:00:00

abstract：BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...

journal_title：BMC molecular biology

authors： Frandsen RJ,Andersson JA,Kristensen MB,Giese H

更新日期：2008-08-01 00:00:00

abstract：BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently f...

journal_title：BMC molecular biology

authors： Li Y,Podsypanina K,Liu X,Crane A,Tan LK,Parsons R,Varmus HE

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human embryonic stem (hES) cells, differentiation itself introduces chan...

journal_title：BMC molecular biology

authors： Vossaert L,O'Leary T,Van Neste C,Heindryckx B,Vandesompele J,De Sutter P,Deforce D

更新日期：2013-09-12 00:00:00

abstract：BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...

journal_title：BMC molecular biology

authors： Passmore M,Nataatmadja M,Fraser JF

更新日期：2009-07-23 00:00:00