解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:Small nucleolar RNAs (snoRNAs) function in guiding 2'-O-methylation and pseudouridylation of ribosomal RNAs (rRNAs) and small nuclear RNAs (snRNAs). In recent years, more and more snoRNAs have been found to play novel roles in mRNA regulation, such as pre-mRNA splicing or RNA editing. In our previous study, ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-019-0128-9
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Physical exercise can improve brain function by altering brain gene expression. The expression mechanisms underlying the brain's response to exercise still remain unknown. miRNAs as vital regulators of gene expression may be involved in regulation of brain genes in response to exercise. However, as yet, very...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-019-0120-4
更新日期:2019-01-15 00:00:00
abstract:BACKGROUND:Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we descr...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-018-0110-y
更新日期:2018-07-27 00:00:00
abstract:BACKGROUND:Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression of α smooth muscle actin (α-SMA) that belongs to the family of the six conserved acti...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-017-0089-9
更新日期:2017-04-27 00:00:00
abstract:BACKGROUND:Oxidative stress can induce cell injury in vascular endothelial cells, which is the initial event in the development of atherosclerosis. Although quantitative real-time polymerase chain reaction (qRT-PCR) has been widely used in gene expression studies in oxidative stress injuries, using carefully validated ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-017-0086-z
更新日期:2017-04-05 00:00:00
abstract:BACKGROUND:Single-stranded DNA-binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA. Human single-stranded DNA-binding protein 1 (hSSB1) is one such protein, with described roles in genome stability maintenance and transcriptional regulation. A...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0077-5
更新日期:2016-12-09 00:00:00
abstract:BACKGROUND:Ataxia telangiectasia mutated (ATM) and TRRAP proteins belong to the phosphatidylinositol 3-kinase-related kinase family and are involved in DNA damage repair and chromatin remodeling. ATM is a checkpoint kinase that is recruited to sites of DNA double-strand breaks where it phosphorylates a diverse range of...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0075-7
更新日期:2016-11-04 00:00:00
abstract:BACKGROUND:The majority of protein isoforms arise from alternative splicing of the encoding primary RNA transcripts. To understand the significance of single splicing events, reliable techniques are needed to determine their incidence. However, existing methods are labour-intensive, error-prone or of limited use. RESU...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0060-1
更新日期:2016-03-15 00:00:00
abstract:BACKGROUND:Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70% of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified using a proteome analysis, the venom gland transcriptome is currently lacking. RESULTS:We constructed a cDNA library from the v...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0059-7
更新日期:2016-03-05 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are short non-protein-coding RNA species that have a regulatory function in modulating protein translation and degradation of specific mRNAs. MicroRNAs are estimated to target approximately 60% of all human mRNAs and are associated with the regulation of all physiological processes. Simila...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0057-9
更新日期:2016-02-09 00:00:00
abstract:BACKGROUND:The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no information is available about Y RNAs in Chinese hamster cells, which have already been used to detect repli...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0053-5
更新日期:2016-01-05 00:00:00
abstract:BACKGROUND:ALL1-fused from chromosome 1q (AF1q), originally considered as an oncogenic factor, has been implicated in neuronal development; however, its upstream regulatory mechanisms in neural system remained elusive. RESULTS:Our study showed that REST (RE1 silencing transcription factor), a key transcription factor ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0043-7
更新日期:2015-09-05 00:00:00
abstract:BACKGROUND:ATPase/Helicases and nucleases play important roles in homologous recombination repair (HRR). Many of the mechanistic details relating to these enzymes and their function in this fundamental and complicated DNA repair process remain poorly understood in archaea. Here we employed Sulfolobus islandicus, a hype...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0030-z
更新日期:2015-02-14 00:00:00
abstract:BACKGROUND:Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS:Rat RGC-5 cells were cultured in plates and H2O2 was added to induce o...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-014-0025-1
更新日期:2014-11-18 00:00:00
abstract:BACKGROUND:Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regula...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-15-10
更新日期:2014-05-25 00:00:00
abstract:BACKGROUND:Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purificatio...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-15-7
更新日期:2014-04-03 00:00:00
abstract:BACKGROUND:Pseudo-nitzschia multiseries Hasle (Hasle) (Ps-n) is distinctive among the ecologically important marine diatoms because it produces the neurotoxin domoic acid. Although the biology of Ps-n has been investigated intensely, the characterization of the genes and biochemical pathways leading to domoic acid bios...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-14-25
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human embryonic stem (hES) cells, differentiation itself introduces chan...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-14-21
更新日期:2013-09-12 00:00:00
abstract:BACKGROUND:RA (retinoic acid) signal pathway has been proved to be required for germ cell meiotic initiation in mammals, aves and amphibians. Stra8 (Stimulated by retinoic acid gene 8) is an important factor in RA signal pathway. However, the role of RA and Stra8 in germ cell meiotic initiation in teleosts is poorly ch...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-14-11
更新日期:2013-05-22 00:00:00
abstract:BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-13-35
更新日期:2012-12-10 00:00:00
abstract:BACKGROUND:The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The present study aimed to identify reference genes as internal controls for gene expression studies by RT-qPC...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-13-22
更新日期:2012-06-29 00:00:00
abstract:BACKGROUND:Small proline rich protein (SPRR) 2A is one of 14 SPRR genes that encodes for a skin cross-linking protein, which confers structural integrity to the cornified keratinocyte cell envelope. New evidence, however, shows that SPRR2A is also a critical stress and wound repair modulator: it enables a variety of ba...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-13-20
更新日期:2012-06-25 00:00:00
abstract:BACKGROUND:Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-12-32
更新日期:2011-08-01 00:00:00
abstract:BACKGROUND:Improving the treatment of renal cell carcinoma (RCC) will depend on the development of better biomarkers for predicting disease progression and aiding the design of appropriate therapies. One such marker may be fatty acid binding protein 7 (FABP7), also known as B-FABP and BLBP, which is expressed normally ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-12-31
更新日期:2011-07-19 00:00:00
abstract:BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-12-30
更新日期:2011-07-12 00:00:00
abstract:BACKGROUND:Members of the Sox gene family isolated from both vertebrates and invertebrates have been proved to participate in a wide variety of developmental processes, including sex determination and differentiation. Among these members, Sox30 had been considered to exist only in mammals since its discovery, and its e...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-98
更新日期:2010-12-11 00:00:00
abstract:BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-69
更新日期:2010-09-14 00:00:00
abstract:BACKGROUND:The L1 cell adhesion molecule (L1CAM) was originally identified as a neural adhesion molecule involved in axon guidance. In many human epithelial carcinomas L1CAM is overexpressed and thereby augments cell motility, invasion and metastasis formation. L1CAM positive carcinomas are associated with bad prognosi...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-64
更新日期:2010-08-27 00:00:00
abstract:BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-58
更新日期:2010-08-13 00:00:00
abstract:BACKGROUND:The Y chromosome of mammals is particularly prone to accumulate genes related to male fertility. However, the high rate of molecular evolution on this chromosome predicts reduced power to the across-species comparative approach in identifying male-specific genes that are essential for sperm production in hum...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-33
更新日期:2010-05-07 00:00:00
abstract:BACKGROUND:Carbonic anhydrases (CAs) are found in many organisms, in which they contribute to several important biological processes. The vertebrate alpha-CA family consists of 16 subfamilies, three of which (VIII, X and XI) consist of acatalytic proteins. These are named carbonic anhydrase related proteins (CARPs), an...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-25
更新日期:2010-03-31 00:00:00
abstract:BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-108
更新日期:2009-12-14 00:00:00
abstract:BACKGROUND:Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as their expression is a...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-99
更新日期:2009-10-30 00:00:00
abstract:BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-98
更新日期:2009-10-28 00:00:00
abstract:BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-97
更新日期:2009-10-25 00:00:00
abstract:BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-95
更新日期:2009-10-16 00:00:00
abstract:BACKGROUND:Retrotransposition is an important evolutionary force for the creation of new and potentially functional intronless genes which are collectively called retrogenes. Many retrogenes are expressed in the testis and the gene products have been shown to actively participate in spermatogenesis and other unique fun...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-74
更新日期:2009-07-25 00:00:00
abstract:BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-72
更新日期:2009-07-23 00:00:00
abstract:BACKGROUND:The human Sex Hormone Binding Globulin (SHBG) gene, located at 17p13.1, comprises, at least, two different transcription units regulated by two different promoters. The first transcription unit begins with the exon 1 sequence and is responsible for the production of plasma SHBG by the hepatocytes, while the ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-59
更新日期:2009-06-17 00:00:00
abstract:BACKGROUND:Modulation of chromatin structure has emerged as a critical molecular device to control gene expression. Histones undergo different post-translational modifications that increase chromatin accessibility to a number of regulatory factors. Among them, histone ubiquitination appears relevant in nuclear processe...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-55
更新日期:2009-06-05 00:00:00