当前位置： SCI文献检索 > BMC MOLECULAR BIOLOGY期刊下所有文献 > Alternative splicing and differential subcellular localization of the rat FGF antisense gene product.

Alternative splicing and differential subcellular localization of the rat FGF antisense gene product.

Abstract：

BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing of the primary FGF-AS mRNA transcript predicts multiple GFG isoforms in many species including rat. In the present study we focused on elucidating the expression and subcellular distribution of alternatively spliced rat GFG isoforms. RESULTS:RT-PCR and immunohistochemistry revealed tissue-specific GFG mRNA isoform expression and subcellular distribution of GFG immunoreactivity in cytoplasm and nuclei of a wide range of normal rat tissues. FGF-2 and GFG immunoreactivity were co-localized in some, but not all, tissues examined. Computational analysis identified a mitochondrial targeting sequence (MTS) in the N-terminus of three previously described rGFG isoforms. Confocal laser scanning microscopy and subcellular fractionation analysis revealed that all rGFG isoforms bearing the MTS were specifically targeted to mitochondria whereas isoforms and deletion mutants lacking the MTS were localized in the cytoplasm and nucleus. Mutation and deletion analysis confirmed that the predicted MTS was necessary and sufficient for mitochondrial compartmentalization. CONCLUSION:Previous findings strongly support a role for the FGF antisense RNA as a regulator of FGF2 expression. The present study demonstrates that the antisense RNA itself is translated, and that protein isoforms resulting form alternative RNA splicing are sorted to different subcellular compartments. FGF-2 and its antisense protein are co-expressed in many tissues and in some cases in the same cells. The strong conservation of sequence and genomic organization across animal species suggests important functional significance to the physical association of these transcript pairs.

journal_name

BMC Mol Biol

journal_title

BMC molecular biology

authors

Zhang SC,MacDonald KA,Baguma-Nibasheka M,Geldenhuys L,Casson AG,Murphy PR

doi

10.1186/1471-2199-9-10

subject

Has Abstract

pub_date

2008-01-23 00:00:00

pages

issn

1471-2199

pii

1471-2199-9-10

journal_volume

pub_type

杂志文章

在线工具

Alternative splicing and differential subcellular localization of the rat FGF antisense gene product.