Abstract:
BACKGROUND:The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two regions that can independently induce transcription when fused to a heterologous DNA binding domain. When expressed in Saccharomyces cerevisiae, each of these regions of E1A also acts as a strong transcriptional activator. This allows yeast to be used as a model system to study mechanisms by which E1A stimulates transcription. RESULTS:Using 81 mutant yeast strains, we have evaluated the effect of deleting components of the ADA, COMPASS, CSR, INO80, ISW1, NuA3, NuA4, Mediator, PAF, RSC, SAGA, SAS, SLIK, SWI/SNF and SWR1 transcriptional regulatory complexes on E1A dependent transcription. In addition, we examined the role of histone H2B ubiquitylation by Rad6/Bre1 on transcriptional activation. CONCLUSION:Our analysis indicates that the two activation domains of E1A function via distinct mechanisms, identify new factors regulating E1A dependent transcription and suggest that yeast can serve as a valid model system for at least some aspects of E1A function.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Yousef AF,Brandl CJ,Mymryk JSdoi
10.1186/1471-2199-10-32subject
Has Abstractpub_date
2009-04-17 00:00:00pages
32issn
1471-2199pii
1471-2199-10-32journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that...
journal_title:BMC molecular biology
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更新日期:2009-05-16 00:00:00
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journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-6-5
更新日期:2005-03-04 00:00:00
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更新日期:2008-01-23 00:00:00
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更新日期:2009-06-05 00:00:00
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pub_type: 杂志文章
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