Characterization of Stra8 in Southern catfish (Silurus meridionalis): evidence for its role in meiotic initiation.

abstract：BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...

journal_title：BMC molecular biology

authors： Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo P

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...

journal_title：BMC molecular biology

authors： Liddiard K,Welch JS,Lozach J,Heinz S,Glass CK,Greaves DR

更新日期：2006-11-29 00:00:00

abstract：BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...

journal_title：BMC molecular biology

authors： Bondino HG,Valle EM

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that...

journal_title：BMC molecular biology

authors： Garcia AC,Gitaí DL,Humann FC,Paçó-Larson ML,Monesi N

更新日期：2011-08-01 00:00:00

abstract：BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...

journal_title：BMC molecular biology

authors： Frandsen RJ,Andersson JA,Kristensen MB,Giese H

更新日期：2008-08-01 00:00:00

abstract：BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...

journal_title：BMC molecular biology

authors： He H,Cheng M,Yang X,Meng J,He A,Zheng X,Li Z,Guo P,Pan Z,Zhan X

更新日期：2009-10-25 00:00:00

abstract：BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...

journal_title：BMC molecular biology

authors： Thiel G,Al Sarraj J,Stefano L

更新日期：2005-01-19 00:00:00

abstract：BACKGROUND:Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. RESULTS:Insertion of a TEV protease cleavage site into Cdc23 allows i...

journal_title：BMC molecular biology

authors： Yang X,Gregan J,Lindner K,Young H,Kearsey SE

更新日期：2005-06-07 00:00:00

abstract：BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...

journal_title：BMC molecular biology

authors： O'Brochta DA,Stosic CD,Pilitt K,Subramanian RA,Hice RH,Atkinson PW

更新日期：2009-12-14 00:00:00

abstract：BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...

journal_title：BMC molecular biology

authors： Jin H,Vacic V,Girke T,Lonardi S,Zhu JK

更新日期：2008-01-14 00:00:00

abstract：BACKGROUND:Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regula...

journal_title：BMC molecular biology

authors： Calvo E,Grzenda A,Lomberk G,Mathison A,Iovanna J,Urrutia R

更新日期：2014-05-25 00:00:00

abstract：BACKGROUND:Among the three functions of DNA, mammalian replication and transcription can be subject to epigenetic imprinting specified by the parental origin of chromosomes, and although there is suggestive indication that this is also true for meiotic recombination, no definitive evidence has yet been reported. RESUL...

journal_title：BMC molecular biology

authors： Ng SH,Madeira R,Parvanov ED,Petros LM,Petkov PM,Paigen K

更新日期：2009-05-13 00:00:00

abstract：BACKGROUND:The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably the most abundant class of enhancers contains A/U-rich sequences. We have tested the influence of SD sequence length and the presence of enhancers on the efficiency o...

journal_title：BMC molecular biology

authors： Vimberg V,Tats A,Remm M,Tenson T

更新日期：2007-10-31 00:00:00

abstract：BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...

journal_title：BMC molecular biology

authors： Dedman AM,Majeed Y,Tumova S,Zeng F,Kumar B,Munsch C,Bateson AN,Wittmann J,Jäck HM,Porter KE,Beech DJ

更新日期：2011-07-12 00:00:00

abstract：BACKGROUND:Alternative splicing is often subjected to complex regulatory control that involves many protein factors and cis-acting RNA sequence elements. One major challenge is to identify all of the protein players and define how they control alternative expression of a particular exon in a combinatorial manner. The M...

journal_title：BMC molecular biology

authors： Fleming VA,Geng C,Ladd AN,Lou H

更新日期：2012-12-10 00:00:00

abstract：BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...

journal_title：BMC molecular biology

authors： Ozber N,Baris I,Tatlici G,Gur I,Kilinc S,Unal EB,Kavakli IH

更新日期：2010-09-14 00:00:00

abstract：BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...

journal_title：BMC molecular biology

authors： Di Lisi R,Picard A,Ausoni S,Schiaffino S

更新日期：2007-09-17 00:00:00

abstract：BACKGROUND:MicroRNAs (miRNAs) are short non-protein-coding RNA species that have a regulatory function in modulating protein translation and degradation of specific mRNAs. MicroRNAs are estimated to target approximately 60% of all human mRNAs and are associated with the regulation of all physiological processes. Simila...

journal_title：BMC molecular biology

authors： Tonge DP,Gant TW

更新日期：2016-02-09 00:00:00

abstract：BACKGROUND:Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as their expression is a...

journal_title：BMC molecular biology

authors： Teste MA,Duquenne M,François JM,Parrou JL

更新日期：2009-10-30 00:00:00

abstract：BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...

journal_title：BMC molecular biology

authors： Mickle KL,Ramanathan S,Rosebrock A,Oliva A,Chaudari A,Yompakdee C,Scott D,Leatherwood J,Huberman JA

更新日期：2007-12-19 00:00:00

abstract：BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...

journal_title：BMC molecular biology

authors： Rutledge RG,Stewart D

更新日期：2008-10-30 00:00:00

abstract：BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...

journal_title：BMC molecular biology

authors： Gervais AL,Gaudreau L

更新日期：2009-03-04 00:00:00

abstract：BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...

journal_title：BMC molecular biology

authors： Yan B,Li D,Gou K

更新日期：2010-08-13 00:00:00

abstract：BACKGROUND:The human Sex Hormone Binding Globulin (SHBG) gene, located at 17p13.1, comprises, at least, two different transcription units regulated by two different promoters. The first transcription unit begins with the exon 1 sequence and is responsible for the production of plasma SHBG by the hepatocytes, while the ...

journal_title：BMC molecular biology

authors： Pinós T,Barbosa-Desongles A,Hurtado A,Santamaria-Martínez A,de Torres I,Morote J,Reventós J,Munell F

更新日期：2009-06-17 00:00:00

abstract：BACKGROUND:Oxidative stress can induce cell injury in vascular endothelial cells, which is the initial event in the development of atherosclerosis. Although quantitative real-time polymerase chain reaction (qRT-PCR) has been widely used in gene expression studies in oxidative stress injuries, using carefully validated ...

journal_title：BMC molecular biology

authors： Li T,Diao H,Zhao L,Xing Y,Zhang J,Liu N,Yan Y,Tian X,Sun W,Liu B

更新日期：2017-04-05 00:00:00

abstract：BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...

journal_title：BMC molecular biology

authors： Zhang SC,MacDonald KA,Baguma-Nibasheka M,Geldenhuys L,Casson AG,Murphy PR

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:Traumatic brain injury models are widely studied, especially through gene expression, either to further understand implied biological mechanisms or to assess the efficiency of potential therapies. A large number of biological pathways are affected in brain trauma models, whose elucidation might greatly benef...

journal_title：BMC molecular biology

authors： Rhinn H,Marchand-Leroux C,Croci N,Plotkine M,Scherman D,Escriou V

更新日期：2008-07-08 00:00:00

abstract：BACKGROUND:Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we descr...

journal_title：BMC molecular biology

authors： Brzek A,Cichocka M,Dolata J,Juzwa W,Schümperli D,Raczynska KD

更新日期：2018-07-27 00:00:00

abstract：BACKGROUND:Duchenne Muscular Dystrophy (DMD) is an X-linked genetic disorder that results in the production of a dysfunctional form of the protein, dystrophin. The mdx5cv mouse is a model of DMD in which a point mutation in exon 10 of the dystrophin gene creates an artificial splice site. As a result, a 53 base pair de...

journal_title：BMC molecular biology

authors： Maguire K,Suzuki T,DiMatteo D,Parekh-Olmedo H,Kmiec E

更新日期：2009-02-23 00:00:00

abstract：BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...

journal_title：BMC molecular biology

authors： Ordinario EC,Yabuki M,Handa P,Cummings WJ,Maizels N

更新日期：2009-10-28 00:00:00