Abstract:
BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in mediating the non-canonical Wnt signaling have been well established. The role of Ror2 in canonical Wnt signaling is not fully understood. RESULTS:Here we report that Ror2 also positively modulates Wnt3a-activated canonical signaling in a lung carcinoma, H441 cell line. This activity of Ror2 is dependent on cooperative interactions with Fzd2 but not Fzd7. In addition, Ror2-mediated enhancement of canonical signaling requires the extracellular CRD, but not the intracellular PRD domain of Ror2. We further provide evidence that the positive effect of Ror2 on canonical Wnt signaling is inhibited by Dkk1 and Krm1 suggesting that Ror2 enhances an Lrp-dependent STF response. CONCLUSION:The current study demonstrates the function of Ror2 in modulating canonical Wnt signaling. These findings support a functional scheme whereby regulation of Wnt signaling is achieved by cooperative functions of multiple mediators.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo Pdoi
10.1186/1471-2199-9-11subject
Has Abstractpub_date
2008-01-23 00:00:00pages
11issn
1471-2199pii
1471-2199-9-11journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Traumatic brain injury models are widely studied, especially through gene expression, either to further understand implied biological mechanisms or to assess the efficiency of potential therapies. A large number of biological pathways are affected in brain trauma models, whose elucidation might greatly benef...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-62
更新日期:2008-07-08 00:00:00
abstract:BACKGROUND:Invitrogen Gateway technology exploits the integrase/att site-specific recombination system for directional cloning of PCR products and the subsequent subcloning into destination vectors. One or three DNA segments can be cloned using Gateway or MultiSite Gateway respectively. A vast number of single-site Gat...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-7-46
更新日期:2006-12-06 00:00:00
abstract:BACKGROUND:Prevalence of fibroproliferative diseases, including chronic kidney disease is rapidly increasing and has become a major public health problem worldwide. Fibroproliferative diseases are characterized by increased expression of α smooth muscle actin (α-SMA) that belongs to the family of the six conserved acti...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-017-0089-9
更新日期:2017-04-27 00:00:00
abstract:BACKGROUND:Cryptochromes (CRYs) are a class of flavoprotein blue-light signaling receptors found in plants and animals, and they control plant development and the entrainment of circadian rhythms. They also act as integral parts of the central circadian oscillator in humans and other animals. In mammals, the CLOCK-BMAL...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-69
更新日期:2010-09-14 00:00:00
abstract:BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prom...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-6-5
更新日期:2005-03-04 00:00:00
abstract:BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-46
更新日期:2009-05-16 00:00:00
abstract:BACKGROUND:hAT elements and V(D)J recombination may have evolved from a common ancestral transposable element system. Extrachromosomal, circular forms of transposable elements (referred to here as episomal forms) have been reported yet their biological significance remains unknown. V(D)J signal joints, which resemble e...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-108
更新日期:2009-12-14 00:00:00
abstract:BACKGROUND:Bothrops colombiensis is a highly dangerous pit viper and responsible for over 70% of snakebites in Venezuela. Although the composition in B. colombiensis venom has been identified using a proteome analysis, the venom gland transcriptome is currently lacking. RESULTS:We constructed a cDNA library from the v...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-016-0059-7
更新日期:2016-03-05 00:00:00
abstract:BACKGROUND:Carbonic anhydrases (CAs) are found in many organisms, in which they contribute to several important biological processes. The vertebrate alpha-CA family consists of 16 subfamilies, three of which (VIII, X and XI) consist of acatalytic proteins. These are named carbonic anhydrase related proteins (CARPs), an...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-25
更新日期:2010-03-31 00:00:00
abstract:BACKGROUND:Transient Receptor Potential Canonical 1 (TRPC1) is a widely-expressed mammalian cationic channel with functional effects that include stimulation of cardiovascular remodelling. The initial aim of this study was to investigate variation in TRPC1-encoding gene transcripts. RESULTS:Extensive TRPC1 transcript ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-12-30
更新日期:2011-07-12 00:00:00
abstract:BACKGROUND:Real-time RT-PCR is the recommended method for quantitative gene expression analysis. A compulsory step is the selection of good reference genes for normalization. A few genes often referred to as HouseKeeping Genes (HSK), such as ACT1, RDN18 or PDA1 are among the most commonly used, as their expression is a...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-99
更新日期:2009-10-30 00:00:00
abstract:BACKGROUND:Sequence periodicity with a period close to the DNA helical repeat is a very basic genomic property. This genomic feature was demonstrated for many prokaryotic genomes. The Escherichia coli sequences display the period close to 11 base pairs. RESULTS:Here we demonstrate that practically only ApA/TpT dinucle...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-5-14
更新日期:2004-08-26 00:00:00
abstract:BACKGROUND:Improving the treatment of renal cell carcinoma (RCC) will depend on the development of better biomarkers for predicting disease progression and aiding the design of appropriate therapies. One such marker may be fatty acid binding protein 7 (FABP7), also known as B-FABP and BLBP, which is expressed normally ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-12-31
更新日期:2011-07-19 00:00:00
abstract:BACKGROUND:The genes coding for Y RNAs are evolutionarily conserved in vertebrates. These non-coding RNAs are essential for the initiation of chromosomal DNA replication in vertebrate cells. However thus far, no information is available about Y RNAs in Chinese hamster cells, which have already been used to detect repli...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-015-0053-5
更新日期:2016-01-05 00:00:00
abstract:BACKGROUND:Transcription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. Th...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-95
更新日期:2009-10-16 00:00:00
abstract:BACKGROUND:The selection of stable and suitable reference genes for real-time quantitative PCR (RT-qPCR) is a crucial prerequisite for reliable gene expression analysis under different experimental conditions. The present study aimed to identify reference genes as internal controls for gene expression studies by RT-qPC...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-13-22
更新日期:2012-06-29 00:00:00
abstract:BACKGROUND:Gene conversion depends upon the same factors that carry out more general process of homologous recombination, including homologous gene targeting and recombinational repair. Among these are the RAD51 paralogs, conserved factors related to the key recombination factor, RAD51. In chicken and other fowl, gene ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-98
更新日期:2009-10-28 00:00:00
abstract:BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-10
更新日期:2008-01-23 00:00:00
abstract:BACKGROUND:Heart and lung transplantation is frequently the only therapeutic option for patients with end stage cardio respiratory disease. Organ donation post brain stem death (BSD) is a pre-requisite, yet BSD itself causes such severe damage that many organs offered for donation are unusable, with lung being the orga...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-72
更新日期:2009-07-23 00:00:00
abstract:BACKGROUND:Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which check...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-119
更新日期:2007-12-21 00:00:00
abstract:BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-9-70
更新日期:2008-08-01 00:00:00
abstract:BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently f...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-2-2
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Oxidative stress can induce cell injury in vascular endothelial cells, which is the initial event in the development of atherosclerosis. Although quantitative real-time polymerase chain reaction (qRT-PCR) has been widely used in gene expression studies in oxidative stress injuries, using carefully validated ...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-017-0086-z
更新日期:2017-04-05 00:00:00
abstract:BACKGROUND:Duchenne Muscular Dystrophy (DMD) is an X-linked genetic disorder that results in the production of a dysfunctional form of the protein, dystrophin. The mdx5cv mouse is a model of DMD in which a point mutation in exon 10 of the dystrophin gene creates an artificial splice site. As a result, a 53 base pair de...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-15
更新日期:2009-02-23 00:00:00
abstract:BACKGROUND:Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. RESULTS:Rat RGC-5 cells were cultured in plates and H2O2 was added to induce o...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-014-0025-1
更新日期:2014-11-18 00:00:00
abstract:BACKGROUND:The Y chromosome of mammals is particularly prone to accumulate genes related to male fertility. However, the high rate of molecular evolution on this chromosome predicts reduced power to the across-species comparative approach in identifying male-specific genes that are essential for sperm production in hum...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-33
更新日期:2010-05-07 00:00:00
abstract:BACKGROUND:It is not clear how foreign DNA molecules insert into the host genome. Recently, we have produced transgenic mice to investigate the role of the fad2 gene in the conversion of oleic acid to linoleic acid. Here we describe an integration mechanism of fad2 transgene by homologous illegitimate random integratio...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-11-58
更新日期:2010-08-13 00:00:00
abstract:BACKGROUND:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays critical functions in many biological processes, including DNA repair and gene transcription. The main function of PARP-1 is to catalyze the transfer of ADP-ribose units from nicotinamide adenine dinucleotide (NAD+) to a large array of acc...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-8-96
更新日期:2007-10-25 00:00:00
abstract:BACKGROUND:Core canonical histones are required in the S phase of the cell cycle to pack newly synthetized DNA, therefore the expression of their genes is highly activated during DNA replication. In mammalian cells, this increment is achieved by both enhanced transcription and 3' end processing. In this paper, we descr...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/s12867-018-0110-y
更新日期:2018-07-27 00:00:00
abstract:BACKGROUND:Human angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis. R...
journal_title:BMC molecular biology
pub_type: 杂志文章
doi:10.1186/1471-2199-10-97
更新日期:2009-10-25 00:00:00