Abstract:
BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prominent surface protein AP65. The bovine trichomonad, Tritrichomonas foetus, adheres poorly to human VECs. Thus, we established a transfection system for heterologous expression of the T. vaginalis AP65 in T. foetus, as an alternative approach to confirm adhesin function for this virulence factor. RESULTS:In this study, we show stable transfection and expression of the T. vaginalis ap65 gene in T. foetus from an episomal pBS-ap65-neo plasmid. Expression of the gene and protein was confirmed by RT-PCR and immunoblots, respectively. AP65 in transformed T. foetus bound to host cells. Specific mAbs revealed episomally-expressed AP65 targeted to the parasite surface and hydrogenosome organelles. Importantly, surface-expression of AP65 in T. foetus paralleled increased levels of adherence of transfected bovine trichomonads to human VECs. CONCLUSION:The T. vaginalis AP65 adhesin was stably expressed in T. foetus, and the data obtained using this heterologous system strongly supports the role of AP65 as a prominent adhesin for T. vaginalis. In addition, the heterologous expression in T. foetus of a T. vaginalis gene offers an important, new approach for confirming and characterizing virulence factors.
journal_name
BMC Mol Bioljournal_title
BMC molecular biologyauthors
Kucknoor AS,Mundodi V,Alderete JFdoi
10.1186/1471-2199-6-5keywords:
subject
Has Abstractpub_date
2005-03-04 00:00:00pages
5issn
1471-2199pii
1471-2199-6-5journal_volume
6pub_type
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journal_title:BMC molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-07-18 00:00:00
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更新日期:2010-03-31 00:00:00
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pub_type: 杂志文章
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更新日期:2006-11-13 00:00:00
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更新日期:2007-05-21 00:00:00
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更新日期:2007-06-22 00:00:00
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更新日期:2001-01-01 00:00:00
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更新日期:2013-05-22 00:00:00
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